Affiliations 

  • 1 Young Researchers and Elite Club, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran; Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran. Electronic address: Hajian@iaug.ac.ir
  • 2 Department of Chemistry, College of Science, Gachsaran Branch, Islamic Azad University, 75818-63876 Gachsaran, Iran
  • 3 Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
Mater Sci Eng C Mater Biol Appl, 2015 Apr;49:769-775.
PMID: 25687007 DOI: 10.1016/j.msec.2015.01.072

Abstract

In this study, an electrochemical sensor was fabricated based on gold nanoparticles/ ethylenediamine/ multi-wall carbon-nanotubes modified gold electrode (AuNPs/en/MWCNTs/AuE) for determination of valrubicin in biological samples. Valrubicin was effectively accumulated on the surface of AuNPs/en/MWCNTs/AuE and produced a pair of redox peaks at around 0.662 and 0.578V (vs. Ag/AgCl) in citrate buffer (pH4.0). The electrochemical parameters including pH, buffer, ionic strength, scan rate and size of AuNPs have been optimized. There was a good linear correlation between cathodic peak current and concentration of valrubicin in the range of 0.5 to 80.0μmolL(-1) with the detection limit of 0.018μmolL(-1) in citrate buffer (pH4.0) and 0.1molL(-1) KCl. Finally, the constructed sensor was successfully applied for determination of valrubicin in human urine and blood serum. In further studies, the different sequences of single stranded DNA probes have been immobilized on the surface of AuNPs decorated on MWCNTs to study the interaction of oligonucleotides with valrubicin.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.