Affiliations 

  • 1 Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia
  • 2 Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: suatmoi@um.edu.my
  • 3 Academy of Sciences Malaysia, 50480, Kuala Lumpur, Malaysia
  • 4 Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071, Barcelona, Spain
  • 5 Department of Biomedical Science, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: khchua@um.edu.my
Microb Pathog, 2023 Apr;177:106059.
PMID: 36878334 DOI: 10.1016/j.micpath.2023.106059

Abstract

Aeromonas dhakensis possesses dual flagellar systems for motility under different environments. Flagella-mediated motility is necessary for biofilm formation through an initial attachment of bacteria to the surface, but this has not been elucidated in A. dhakensis. This study investigates the role of polar (flaH, maf1) and lateral (lafB, lafK and lafS) flagellar genes in the biofilm formation of a clinical A. dhakensis strain WT187 isolated from burn wound infection. Five deletion mutants and corresponding complemented strains were constructed using pDM4 and pBAD33 vectors, respectively, and analyzed for motility and biofilm formation using crystal violet staining and real-time impedance-based assays. All mutants were significantly reduced in swimming (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.