The regulation of Cytochrome f by mannose treatment in broccoli and its relationship with programmed cell death in tobacco BY-2 cells

Zhao S 1 , Chen J 1 , Cao S 2 , Wang H 1 , Chen H 3 , Wei Y 1 Show all authors , Chen Y 1 , Shao X 1 , Xu F 4

Affiliations 

  • 1 College of Food Science and Engineering, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo, 315800, China
  • 2 College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, 315100, China
  • 3 Food Science Institute, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Postharvest Preservation and Processing of Vegetables (Co-construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Hangzhou, 310021, China
  • 4 College of Food Science and Engineering, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo University, Ningbo, 315800, China. Electronic address: xufeng1@nbu.edu.cn
Plant Physiol Biochem, 2024 Mar;208:108480.
PMID: 38437751 DOI: 10.1016/j.plaphy.2024.108480

Abstract

It is well established that programmed cell death (PCD) occurred in broccoli during postharvest senescence, but no studies have been conducted on the regulation of broccoli cytochrome f by mannose treatment and its relationship with PCD. In this study, we treated broccoli buds with mannose to investigate the changes in color, total chlorophyll content, gene expression related to chlorophyll metabolism, chloroplast structure, and cytochrome f determination during postharvest storage. In addition, to investigate the effect of cytochrome f on PCD, we extracted cytochrome f from broccoli and treated Nicotiana tabacum L. cv Bright Yellow 2 (BY-2) cells with extracted cytochrome f from broccoli at various concentrations. The results showed that cytochrome f can induce PCD in tobacco BY-2 cells, as evidenced by altered cell morphology, nuclear chromatin disintegration, DNA degradation, decreased cell viability, and increased caspase-3-like protease production. Taken together, our study indicated that mannose could effectively delay senescence of postharvest broccoli by inhibiting the expression of gene encoding cytochrome f which could induce PCD.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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