Displaying publications 1 - 20 of 22 in total

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  1. Cao SG, Sim KY, Goh SH
    Nat Prod Lett, 2001;15(5):291-7.
    PMID: 11841112
    Two new biflavonoids, pyranoamentoflavone 7-methyl ether (1) and pyranoamentoflavone 4'-methyl ether (2), have been isolated from the leaves of Calophyllum venulosum. The structures of these two new compounds were elucidated by spectroscopic data.
  2. Gui F, Zhou J, Sun X, Lu J, Cao S
    PLoS One, 2025;20(1):e0316138.
    PMID: 39746108 DOI: 10.1371/journal.pone.0316138
    Extending product life is one of the effective ways to reduce the waste of resources. However, many unsatisfactory products are scrapped because of a lack of adequate performance. The product should be improved and upgraded innovatively, and the existing upgradable products may create more economic benefits for the longer product life cycles. This paper proposed a product innovative design and product upgrade employing an Extenics-TRIZ Integrated requirement-function-principle-structure (RFPS) model, which aims at complex requirement flexibility with easy-to-use design process when the product needs a redesign. Here, the requirement flexibility refers to the ability of a design object to adapt its design levels. There are two design strategies: the extension analysis methods are utilized to map the top-level requirements to functions, principles, and structures requirements, and then the TRIZ is used to handle the design problems according to the objects on different levels. This design knowledge is summarized as RFPS, and it can be reused in computer-aided innovation further. A case study for a cutting table is illustrated to the innovation and upgrade, and it indicates the effectiveness for designers to implement the design methodology.
  3. Cao S, Liu J, Wang Z, Geok SK
    Front Physiol, 2024;15:1391394.
    PMID: 38784117 DOI: 10.3389/fphys.2024.1391394
    Background: Evidence suggests that functional training (FT) positively impacts physical fitness and sports performance. However, a systematic review addressing the effects of FT on basketball players remains absent. This systematic review aims to explore the influence of FT on physical fitness and skill-related performance in basketball players. Methods: We searched six databases: Web of Science, Scopus, PubMed, China National Knowledge Infrastructure (CNKI), EBSCOhost, and Google Scholar. The search utilized a combination of keywords related to FT, physical fitness, and basketball. The Eligibility Criteria of Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) guidelines were followed in this systematic review. Results: 11 studies were ultimately included in this review, collectively recruiting 333 basketball players. These studies demonstrated that FT significantly improved muscle strength, linear speed, cardiovascular endurance, flexibility, balance, and muscular endurance. However, the effects of FT on power, change-of-direction speed, and basketball-related performance were inconsistent. Most studies showed FT significantly improves these three variables, but a small number of studies did not find positive effects of FT using specific tests including standing long jump, Sargent jump, touch high, lane agility, lateral shuffle, dribbling line drill, and free-throw tests. Conclusion: FT is an effective training method for enhancing physical fitness including muscle strength, linear speed, cardiovascular endurance, flexibility, balance, and muscular endurance. However, the effects of FT on power, change-of-direction speed, and basketball-related performance were divergent. Some tests were not improved after FT potentially due to the short program lengths and training session durations, varied athletic levels of players examined, and different foci of the FT exercises administered. The collective evidence suggests FT programs, especially the specific exercises prescribed, should be tailored to the desired training objectives. More studies investigating the effects of FT on physical fitness and basketball-related performance with established tests are encouraged in the future to expand the current evidence base. Systematic Review Registration: https://inplasy.com/, Identifier INPLASY202360072.
  4. Cao S, Liu J, Geok SK, Sun H, Wang X
    PLoS One, 2024;19(12):e0306815.
    PMID: 39739709 DOI: 10.1371/journal.pone.0306815
    OBJECTIVE: The detrimental effects of mental fatigue (MF) have been established in sports, such as soccer, volleyball, and basketball. Mindfulness interventions are considered a promising method to help players counteract MF, but whether it could improve basketball tactical performance after MF in competition is not clear. This study aims to investigate the effect of brief mindfulness intervention on basketball tactical performance under MF.

    METHOD: This study employed a cluster randomized controlled trial (cRCT) design. It involved 54 male basketball players aged 18 to 24 from three universities. The participants were randomly assigned to one of three groups: control group (CG), mental fatigue group (MFG), and mental fatigue-mindfulness group (MF-MG). Players in the MFG and MF-MG underwent a 30-minute Stroop task to induce MF. Subsequently, players in the MF-MG engaged in a 30-minute audio mindfulness intervention. Basketball tactical performance was assessed in the small side games (SSG).

    RESULTS: There were no significant differences in total tactics observed across groups and over time. However, when examining specific tactical sub-variables, significant differences were found in ball reversal, dribble penetration into the key area, and off-ball screen between the CG and MFG in the post-test. Furthermore, significant differences were noted in ball reversal, dribble penetration into the key area, on-ball screen, and off-ball screen between the MFG and MF-MG in the post-test.

    CONCLUSION: The basketball tactical performances, particularly in areas such as ball reversal, dribble penetration into the key area, on-ball screen, and off-ball screen, were negatively impacted by MF. Notably, the brief mindfulness intervention effectively restored these performance aspects. This suggests that coaches and trainers should place increased emphasis on players' mental well-being and consider incorporating brief mindfulness interventions into their training programs. More studies that investigate mindfulness intervention on the comprehensive aspects of basketball performance should be focused on in the future.

  5. Cao S, Li Z, Wang Z, Geok SK, Liu J
    J Sports Sci Med, 2025 Mar;24(1):31-51.
    PMID: 40046212 DOI: 10.52082/jssm.2025.31
    This review aims to evaluate the effectiveness of HIIT on basketball players' physical fitness and skill-related performance. This study adhered to the PRISMA guidelines and included randomized controlled trials (RCTs) that investigated the effects of HIIT on basketball players. The databases searched included Web of Science, Scopus, PubMed, and SPORTDiscus (up to 4 March 2024). The meta-analysis used a random-effects model, with effect sizes (ES) calculated for various performance outcomes. A total of 15 studies, with a low risk of bias or some concerns of bias, including 369 players (130 females, 239 males) at the developmental level, national level, and international level, were included in the systematic review, with 7 of these included in the meta-analysis. The systematic review indicated that HIIT significantly improved cardiovascular endurance, power, change of direction (COD) ability, linear sprint, and basketball skill-related performance. However, the effects on certain physical aspects such as VO2max, the Yo-Yo intermittent recovery test level 1 (Yo-Yo IR 1), jump tests, ball throw test, 20-m COD sprint test, T-test, 20-m linear sprint, and basketball-specific skills such as shooting accuracy and passing were inconsistent. The meta-analysis revealed a very large effect on the Yo-Yo IR 1 (ES = 2.32; p = 0.000), a moderate effect on VO2max (ES = 0.90; p = 0.000), T-test performance (ES = 0.91; p = 0.000), and CMJ height (ES = 0.76; p = 0.000), and a small effect on the 20-m sprint test (ES = 0.59; p = 0.006). HIIT appears to be an effective training method for improving general physical fitness and certain basketball-specific skills, particularly endurance, power, and agility. However, its impact on more skill-specific aspects, such as shooting accuracy and passing, requires further investigation. Coaches should consider supplementing HIIT with targeted skill training and carefully plan its timing, ideally incorporating HIIT during pre-season or off-season periods for optimal effectiveness. Further research is needed to explore the differential effects of HIIT across various age groups and playing levels.
  6. Cao S, Geok SK, Roslan S, Sun H, Lam SK, Qian S
    Front Psychol, 2021;12:819081.
    PMID: 35082736 DOI: 10.3389/fpsyg.2021.819081
    Mental fatigue (MF) is a psycho-biological state that impairs sports-related performances. Recently, it has been proved that MF can affect basketball performance. However, a systematic overview detailing the influences of MF on basketball performance is still lacking. This study aims to investigate the effects of MF on the physical, technical, tactical, and cognitive performance of basketball. We used the databases of PubMed, Web of Science, SPORTDiscus, Scopes, and CKNI for articles published up to 31 May 2021. The articles included in this study were projected to test whether MF influences basketball athlete performance. Only experimental design studies were selected, and the control condition was without MF. Finally, seven articles fit the inclusion criteria. The results imply that MF impairs the technical aspects of basketball (free throws, three-point shots, and total turnover) and the players' cognitive [take-the-first (TTF) heuristics and decision-making] performance, which results in athletes not using their techniques skillfully and being unable to make practical decisions during critical points in the game. In addition to that, the influences of MF on physical and tactical performance have not been studied. Further studies should look into comprehensive research on the influences of MF on basketball performance, especially on a player's physical and tactical performance. Systematic Review Registration: [https://inplasy.com/] [INPLASY2021100017].
  7. Lou J, Wu C, Wang H, Cao S, Wei Y, Chen Y, et al.
    Food Chem, 2023 May 15;408:135185.
    PMID: 36525725 DOI: 10.1016/j.foodchem.2022.135185
    The effect of melatonin treatment on the carotenoid metabolism in broccoli florets during storage was explored. The results indicated that 100 µmol/L of melatonin maintained the sensory quality of broccoli florets, which retarded the increase of the L* value and the decrease of the H value. Melatonin treatment increased the activities of tryptophan decarboxylase (TDC), tryptamine 5-hydroxylase (T5H), serotonin N-acetyltransferase (SNAT) and N-acetylserotonin methyltransferase (ASMT), leading to the enrichment of endogenous melatonin content in broccoli florets. Meanwhile, the treatment inhibited the concentrations of β-carotene, β-cryptoxanthin, zeaxanthin and lutein, which was beneficial in delaying the yellowing of broccoli. In addition, a series of carotenoid biosynthetic genes such as BoPSY, BoPDS, BoZDS, BoLCYβ and BoZEP was also suppressed by melatonin. Further analysis revealed that the lower carotenoid content and the down-regulated BoNCED expression in treated broccoli resulted in less accumulation of abscisic acid precursors, inhibiting abscisic acid production during the yellowing process.
  8. Hou Y, Ren H, Wang K, Cao S, Zheng Y, Wei Y, et al.
    J Food Sci Technol, 2022 Feb;59(2):552-561.
    PMID: 35185175 DOI: 10.1007/s13197-021-05039-y
    Effect of fresh-cut procedure on the accumulation of GABA in carrots via γ-aminobutyric acid (GABA) shunt and polyamines degradation pathway was investigated. Results showed that fresh-cut processing enhanced glutamate decarboxylase (GAD) activity and expression levels of DcGAD1 and DcGAD2, while reduced GABA transaminase (GABA-T) activity and DcGABA-T1 expression level, which induced the more glutamate (Glu) conversion to GABA. Polyamines (PAs) in shredded carrots were significantly lower than the whole, due to the elevated activities of diamine oxidase (DAO), polyamine oxidase (PAO) and aminoaldehyde dehydrogenase (AMADH) and DcPAO expression level, which indicated that the polyamines degradation pathway was activated and more PAs were converted to GABA. These results suggested that fresh-cut procedure can induce the accumulation of GABA through activating GABA shunt and polyamines degradation pathway. Besides, fresh-cut processing treatment did not have much adverse effect on the organoleptic quality of carrots.

    SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at (10.1007/s13197-021-05039-y).

  9. Chen H, Cao S, Chen J, Wang H, Wei Y, Chen Y, et al.
    J Plant Physiol, 2024 Sep;300:154297.
    PMID: 38945071 DOI: 10.1016/j.jplph.2024.154297
    Programmed cell death (PCD) is a genetically regulated process of cell suicide essential for plant development. The 'malate valve' is a mechanism that ensures redox balance across different subcellular compartments. In broccoli, the BomMDH1 gene encodes malate dehydrogenase in mitochondria, a critical enzyme in the 'malate circulation' pathway. This study investigates the functional role of BomMDH1 in malate (MA)-induced apoptosis in bright yellow-2 (BY-2) suspension cells. Findings revealed that transgenic cells overexpressing BomMDH1 showed enhanced viability under MA-induced oxidative stress compared to wild-type (WT) cells. Overexpression of BomMDH1 also reduced levels of reactive oxygen species (ROS), hydrogen peroxide (H2O2), and malondialdehyde (MDA), while increasing the expression of antioxidant enzyme genes such as NtAPX, NtAOX1a, NtSOD, and NtMDHAR. Additionally, treatment with salicylhydroxamic acid (SHAM), a characteristic inhibitor of mitochondrial respiration, further improved the anti-apoptotic activity of BY-2 cells. Overall, these results highlighted the function of the BomMDH1 gene and the potential of SHAM treatment in mitigating oxidative stress in BY-2 suspension cells.
  10. Zhang J, Zhao J, Zuo X, You W, Ru X, Xu F, et al.
    Food Chem, 2024 Jun 15;443:138545.
    PMID: 38306904 DOI: 10.1016/j.foodchem.2024.138545
    The effects of exogenous glutamate treatment on the quality attributes, γ-aminobutyric acid (GABA) shunt, phenylpropanoid pathway, and antioxidant capacity of fresh-cut carrots were investigated. Results showed that glutamate treatment suppressed the increases in lightness and whiteness values, inhibited the degradation of total carotenoids and maintained better flavor and taste in fresh-cut carrots. Moreover, glutamate treatment rapidly promoted the activities of glutamate decarboxylase and GABA transaminase, thus improving the GABA content. It also significantly enhanced the activities of phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, and 4-coumarate coenzyme A ligase and promoted the accumulation of total phenolics as well as the main individual phenolic compounds, including chlorogenic and caffeic acid. In addition, glutamate application activated the reactive oxygen system-related enzyme including peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase activities to maintain higher antioxidant capacity in fresh-cut carrots. These results demonstrated that exogenous glutamate treatment maintained better nutritional quality and alleviated color deterioration by accelerating the accumulation of GABA and phenolics and enhancing the antioxidant capacity in fresh-cut carrots.
  11. You W, Zhang J, Ru X, Xu F, Wu Z, Jin P, et al.
    Plant Physiol Biochem, 2024 Jan;206:108217.
    PMID: 38039581 DOI: 10.1016/j.plaphy.2023.108217
    The effect of calcium chloride (CaCl2) treatment on γ-aminobutyric acid (GABA) accumulation in fresh-cut cantaloupe and the involved mechanisms were investigated. The result showed that 1% (w/v) CaCl2 treatment increased GABA content and activities of glutamate decarboxylase (GAD) and succinate semialdehyde dehydrogenase (SSADH), while decreased glutamate (Glu) content and GABA transaminase (GABA-T) activities in fresh-cut cantaloupe. CmCML11 and CmCAMTA5 expressions of CaCl2-treated fruit increased by 187.4% and 165.6% than control fruit in the initial 6 h. Besides, expressions of GABA shunt genes, including CmGAD1, CmGAD2, CmGABA-T and CmSSADH were also up-regulated by CaCl2 treatment during early storage. Moreover, acting as a transcriptional activator, CmCAMTA5 could bind to the CG-box in promoters of CmGAD1, CmGABA-T and CmSSADH and activate their transcription. Furthermore, the interaction between CmCML11 and CmCAMTA5 could enhance the transcriptional activation on GABA shunt genes which were regulated by CmCAMTA5. Collectively, our findings revealed that CaCl2 treatment promoted GABA accumulation in fresh-cut cantaloupe via the combined effect of CmCML11 and CmCAMTA5 in the regulation of expressions of CmGAD1, CmGABA-T, and CmSSADH in GABA shunt.
  12. You W, Wang C, Zhang J, Ru X, Xu F, Wu Z, et al.
    Food Chem, 2024 Jul 15;446:138866.
    PMID: 38430769 DOI: 10.1016/j.foodchem.2024.138866
    Fresh-cut potatoes are prone to surface browning and physiological degradation. Chlorogenic acid (CGA), a natural phenolic antioxidant, has demonstrated preservative properties in various postharvest products. However, the underlying mechanisms of its application on maintaining quality remain unclear. Therefore, the effect of exogenous CGA treatment on quality deterioration of potato slices and the mechanisms involved were investigated. Results revealed CGA treatment retarded the browning coloration, suppressed microbial growth and inhibited the declines in starch, and ascorbic acid contents in potato slices. Meanwhile, the treatment activated the phenylpropanoid pathway but decreased the activities of phenolic decomposition-related enzymes such as polyphenol oxidase (PPO) and tyrosinase and downregulated StPPO expression. Moreover, the treated slices exhibited reduced accumulation of reactive oxygen species and increased activity of antioxidant enzymes. Additionally, they displayed enhanced 2,2-diphenyl-1-picrylhydrazyl radicals scavenging capacity and higher ATP levels. Therefore, these findings indicated that CGA treatment was effective for quality maintenance and antioxidant capacity enhancement in fresh-cut potatoes, thereby providing potential strategies for the preservation and processing of fresh-cut produce.
  13. Zhao S, Chen J, Cao S, Wang H, Chen H, Wei Y, et al.
    Plant Physiol Biochem, 2024 Mar;208:108480.
    PMID: 38437751 DOI: 10.1016/j.plaphy.2024.108480
    It is well established that programmed cell death (PCD) occurred in broccoli during postharvest senescence, but no studies have been conducted on the regulation of broccoli cytochrome f by mannose treatment and its relationship with PCD. In this study, we treated broccoli buds with mannose to investigate the changes in color, total chlorophyll content, gene expression related to chlorophyll metabolism, chloroplast structure, and cytochrome f determination during postharvest storage. In addition, to investigate the effect of cytochrome f on PCD, we extracted cytochrome f from broccoli and treated Nicotiana tabacum L. cv Bright Yellow 2 (BY-2) cells with extracted cytochrome f from broccoli at various concentrations. The results showed that cytochrome f can induce PCD in tobacco BY-2 cells, as evidenced by altered cell morphology, nuclear chromatin disintegration, DNA degradation, decreased cell viability, and increased caspase-3-like protease production. Taken together, our study indicated that mannose could effectively delay senescence of postharvest broccoli by inhibiting the expression of gene encoding cytochrome f which could induce PCD.
  14. Cao S, Wang Z, Guo J, Geok SK, Sun H, Liu J
    Front Physiol, 2024;15:1386788.
    PMID: 39027901 DOI: 10.3389/fphys.2024.1386788
    OBJECTIVE: This study aims to analyze the effects of plyometric training (PT) on physical fitness and skill-related performance in female basketball players.

    METHOD: Five databases, including Web of Science, Scopus, PubMed, EBSCOhost, and Google Scholar, were used to select articles published up to 20 December 2023, using a combination of keywords related to PT and female basketball players. The risk of bias and the certainty of evidence in included articles were assessed using the Cochrane risk of bias (RoB2) tool and "The Grading of Recommendations Assessment, Development, and Evaluation" (GRADE).

    RESULTS: Ten studies were included for the systematic review, and eight for the meta-analysis, totalling 246 female basketball players aged 14.5-22.5 years. Most of these players were highly trained. Most of the included studies exhibited concerns regarding the risk of bias. The PT programs lasted 4-8 weeks, conducted 2-3 sessions per week, with sessions lasting 20-90 min and including 29-190 jumps. In the systematic review, most studies showed that PT significantly improved performance in countermovement jump (CMJ), squat jump (SJ), Sargent jump, standing long jump, lateral hop, medicine ball throw, t-Test, Illinois agility, lane agility drill, linear 20-m sprint, stable and dynamic leg balance, dribbling, passing, shooting, and various basketball-specific tests, as well as increased muscle volume and thigh cross-sectional area. However, some studies showed PT to induce no significant changes in performance during CMJ, t-Test, Illinois agility, knee extensor/flexor strength, linear sprint, and single leg balance tests. In the meta-analysis, CMJ height (ES = 0.37; p = 0.036), vertical jump (VJ) peak power (ES = 0.57; p = 0.015), VJ peak velocity (ES = 0.26; p = 0.004), and t-Test performance time (ES = 0.32; p = 0.004) were significantly improved with small effects following PT.

    CONCLUSION: The effect of PT on performance in female basketball players was mixed. Most studies indicated that PT could improve various measures of physical fitness and skill-related performance, but performance remained unchanged in some tests. More studies with established tests are needed to investigate the effect of PT on female basketball players in the future.

    SYSTEMATIC REVIEW REGISTRATION: https://inplasy.com/, Identifier INPLASY2023120078.

  15. Akbar S, Soh KG, Jazaily Mohd Nasiruddin N, Bashir M, Cao S, Soh KL
    Front Physiol, 2022;13:939042.
    PMID: 36213248 DOI: 10.3389/fphys.2022.939042
    Objective: This review study aimed to assess the impact of neuromuscular training (NT) on athletes' physical fitness in sports. Methods: Three independent reviewers conducted a literature search in various databases: EBSCOHOST, PubMed, WOS, Mendeley, Scopus, ProQuest, Science Direct, additional references, and Google Scholar. The methodological quality was examined using Lubans' predetermined methods, and data that included trials were excluded. Results: This review included 18 well-conducted systematic studies from 144 relevant publications. These studies were reviewed and have been given a score of 6. Medium-risk studies were scored 3 or 4, while low-risk studies were scored 5 or 6. None of the studies had a high-risk bias. The NT intervention revealed that balance (n = 10) was the main characteristic of physical fitness that was evaluated, followed by agility (n = 6), muscular strength (n = 4), speed (n = 5), endurance and muscular power (n = 2). Subsequently, most studies used an intervention such as plyometric and strength training exercises to improve agility, balance, and muscular strength among athletes. Conclusion: This review implicated that (NT) focuses on exercises that enhance motor skills which aid athletes in moving their bodies according to their situational needs. The athletes' slower and faster directions influence their agility, muscular strength, and balance, essential for player performance. It is recommended for future research to investigate the effects of neuromuscular training (length of 12-weeks, frequency of 3 days per week and 90-min duration) on physical fitness components (coordination, reaction-time, flexibility, cardiovascular fitness, cardiorespiratory fitness and body composition) that are essential for all ages of male and female athletes in all sports.
  16. Wang X, Soh KG, Deng N, Zhang D, Cao S, Samsudin S
    Heliyon, 2024 Jan 30;10(2):e24087.
    PMID: 38293332 DOI: 10.1016/j.heliyon.2024.e24087
    This study aims to analyse the effects of functional training on muscle strength, jumping, and functional movement screen in wushu athletes.

    METHODS: This study followed the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). A systematic search of electronic databases was also conducted, including EBSCOhost, Scopus, PubMed, Web of Science, CNKI, Google Scholar, and Wanfang. The Physiotherapy Evidence Database (PEDro) scale was an effective indicator to evaluate the quality of studies included in the systematic review.

    RESULTS: This systematic review included 474 participants aged 8-24 years old. The intervention period for most studies was 12 weeks. Among the included studies, 6 focused on muscle strength, 4 on jumping performance, and 11 on functional movement screen.

    CONCLUSION: These articles have been analysed, and the positive impact of functional training interventions on muscle strength, jumping, and functional movement screen of wushu athletes has been verified.

  17. Ru X, You W, Zhang J, Xu F, Wu Z, Jin P, et al.
    Food Chem, 2024 Nov 30;459:140420.
    PMID: 39024869 DOI: 10.1016/j.foodchem.2024.140420
    The effects of γ-aminobutyric (GABA) on enzymatic browning, storage quality, membrane and reactive oxygen species (ROS) metabolism in fresh-cut stem lettuce were investigated. The results illustrated that GABA treatment delayed browning degree, polyphenol oxidase (PPO) activity and the expression of LsPPO. Meanwhile, higher chlorophyll and ascorbic acid contents were exhibited in GABA-treated stem lettuce, as well as the slower microbial propagation. Further investigation revealed that exogenous GABA application declined malondialdehyde content, electrolyte leakage and the enzyme activities of membrane metabolism, and the expression levels of related genes were also downregulated. In addition, GABA treatment scavenged ROS and strengthened the enzyme activities of ROS metabolism, as well as the expression levels of corresponding genes. Taken together, these findings implied that the repressed enzymatic browning and microbial propagation in GABA-treated stem lettuce were due to the inhibition of ROS accumulation, enhancement of membrane stability and increased resistance to oxidation.
  18. Ru X, You W, Zhang J, Xu F, Wu Z, Jin P, et al.
    Int J Biol Macromol, 2024 Dec;283(Pt 2):137729.
    PMID: 39551293 DOI: 10.1016/j.ijbiomac.2024.137729
    GABA is able to increase resistance to biotic and abiotic stresses in fresh-cut fruits and vegetables. Therefore, the objective of this research was to explore the potential regulatory mechanisms of γ-aminobutyric acid (GABA) accumulation in fresh-cut stem lettuce following GABA treatment. The evidence showed that exogenous GABA stimulated the GABA shunt by elevating glutamate levels, the activities of GABA transaminase (GABA-T) and glutamate decarboxylase (GAD). Similarly, GABA stimulated polyamine metabolism by increasing the activities of 4-amino aldehyde dehydrogenase (AMADH), polyamine oxidase (PAO) and diamine oxidase (DAO), as well as elevating free polyamines, arginine and ornithine levels. Subsequently, GABA application up-regulated the expression of GABA shunt genes and polyamine metabolism genes. Additionally, GABA treatment resulted in the down-regulation of LsMYB44 and LsWRKY12 expressions. Notably, LsMYB44 bound to MYB binding sites in the LsGAD, LsGABAT1, LsADC1, LsPAO2, LsALDH7B4 promoters and repressed transcription of these genes. The interaction between LsMYB44 and LsWRKY12 was associated with the transcriptional repression of polyamine metabolism and GABA shunt genes by LsMYB44. In conclusion, LsMYB44 and LsWRKY12 downregulated the transcription of key genes of GABA shunt and polyamine metabolism in fresh-cut lettuce. This downregulation, however, was alleviated by the application of GABA, thereby promoting endogenous GABA accumulation.
  19. Pan X, Soh KG, Jaafar WMW, Soh KL, Deng N, Cao S, et al.
    PLoS One, 2025;20(2):e0310403.
    PMID: 39977446 DOI: 10.1371/journal.pone.0310403
    Mental fatigue, or cognitive fatigue, is a multi-aspect of exhaustion resulting from prolonged engagement in mentally demanding tasks, characterized by diminished energy, mental exhaustion, and distraction, which can adversely impact various aspects of golfers' performance. However, there are still limited systematic reviews on the interaction between mental fatigue and athletes' performance in golf. This study aims to provide a comprehensive analysis of the correlation between mental fatigue and golf and demonstrate the current state of research and characterization of research in the field. The systematic review was conducted using a PRISMA flow chart, with thorough literature searches across PubMed, Web of Science, SPORTDiscus, Scopus, and China National Knowledge Infrastructure (CNKI) databases. The quality of the literature was assessed using Qualsyst. The study summarized findings from 10 articles on the interaction between mental fatigue and athletes' performance in golf. It indicates that mental fatigue is influenced by factors such as duration of play and walking distance, with prolonged golf tasks inducing mental fatigue. Mental fatigue directly affects golf performance, including the overall score for 18 holes, iron club accuracy, drive distance, and especially impacts putting performance. However, the results of these studies are limited and one-sided because studies conducted on driving ranges or in laboratories focus only on putting performance and ignore other golf skills. The study on the effects of mental fatigue on iron accuracy and driving distance was conducted under competitive conditions on an outdoor golf course, making it impossible to eliminate confounding factors. The lack of intervention studies on other specific golf skills may limit a comprehensive understanding of the impact of mental fatigue on golf performance. Trial registration Systematic Review Registration: [https://inplasy.com/][INPLASY202410111].
  20. Ding C, Kim Geok S, Sun H, Roslan S, Cao S, Zhao Y
    PLoS One, 2025;20(1):e0316252.
    PMID: 39752412 DOI: 10.1371/journal.pone.0316252
    INTRODUCTION: Mental fatigue, a psychobiological state induced by prolonged and sustained cognitive tasks, impairs both cognitive and physical performance. Several studies have investigated strategies to counteract mental fatigue. However, potential health risks and contextual restrictions often limit these strategies, which hinder their practical application. Due to its noninvasive and portable nature, music has been proposed as a promising strategy to counteract mental fatigue. However, the effects of music on performance decrements vary with different music styles. Synthesizing studies that systematically report music style and its impact on counteracting performance decrements is crucial for theoretical and practical applications.

    OBJECTIVES: This review aims to provide a comprehensive systematic analysis of different music styles in counteracting mental fatigue and their effects on performance decrements induced by mental fatigue. Additionally, the mechanisms by which music counteracts mental fatigue will be discussed.

    METHODS: A comprehensive search was conducted across five databases-Web of Science, PubMed, SCOPUS, SPORTDiscus via EBSCOhost, and the Psychological and Behavioral Sciences Collection via EBSCOhost-up to November 18, 2023. The selected studies focused solely on music interventions, with outcomes including subjective feelings of mental fatigue, physiological markers, and both cognitive and behavioral performance.

    RESULTS: Nine studies met the predetermined criteria for inclusion in this review. The types of music interventions that counteract mental fatigue include relaxing, exciting, and personal preference music, all of which were associated with decreased subjective feelings of mental fatigue and changes in objective physiological markers. Cognitive performance, particularly in inhibition and working memory tasks impaired by mental fatigue, was countered by both relaxing and exciting music. Exciting music was found to decrease reaction time more effectively than relaxing music in working memory tasks. The physiological marker of steady-state visually evoked potential-based brain-computer interface (SSVEP-BCI) amplitude increased, confirming that exciting music counteracts mental fatigue more effectively than relaxing music. Behavioral performance in tasks such as arm-pointing, the Yo-Yo intermittent test, and the 5 km time-trial, which were impaired by mental fatigue, were counteracted by personal preference music.

    CONCLUSION: Relaxing music, exciting music, and personal preference music effectively counteract mental fatigue by reducing feelings of fatigue and mitigating performance decrements. Individuals engaged in mentally demanding tasks can effectively counteract concurrent or subsequent cognitive performance decrements by simultaneously listening to relaxing or exciting music without lyrics or by using music during recovery from mental fatigue. Exciting music is more effective than relaxing music in counteracting mental fatigue. Personal preference music is effective in counteracting behavioral performance decrements in motor control and endurance tasks. Mentally fatigued individuals could apply personal preference music to counteract subsequent motor control performance decrements or simultaneously listen to it to counteract endurance performance decrements. Future studies should specify and examine the effects of different music genres, tempos, and intensities in counteracting mental fatigue. Additionally, the role of music in counteracting mental fatigue in contexts such as work productivity, traffic accident risk, and sports requires further investigation, along with the underlying mechanisms.

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