Affiliations 

  • 1 Genetic Medicine Research Centre; Universiti Putra Malaysia; Selangor, Malaysia; Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia
  • 2 Medical Genetics Laboratory; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia
  • 3 Department of Medical Microbiology and Parasitology; Faculty of Medicine and Health Sciences; Universiti Putra Malaysia; Selangor, Malaysia
Hum Vaccin Immunother, 2013 Oct;9(10):2222-7.
PMID: 24051430 DOI: 10.4161/hv.25325

Abstract

Polymeric carriers in the form of cellulose acetate phthalate (CAP) and alginate (ALG) microspheres were used for encapsulation of plasmid DNA for oral mucosal immunization. Access into the intestinal mucosa by pVAX1 eukaryotic expression plasmid vectors carrying gene-coding sequences, either for the cholera enterotoxin B subunit (ctxB) immunostimulatory antigen or the green fluorescent protein (GFP), delivered from both types of microsphere carriers were examined in orally immunized BALB/c mice. Demonstration of transgene protein expression and IgA antibody responses at local mucosal sites suggest immunological response to a potential oral DNA vaccine formulated within the microsphere carriers.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.