Affiliations 

  • 1 National Bureau of Fish Genetic Resources (NBFGR), Lucknow, UP, 226002, India. kamal_biotec@yahoo.com
  • 2 Central Institute of Fisheries Education, Versova, Andheri (W), Mumbai, 400061, India
  • 3 Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India
  • 4 National Bureau of Fish Genetic Resources (NBFGR), Lucknow, UP, 226002, India
Fish Physiol. Biochem., 2012 Aug;38(4):1035-1045.
PMID: 22203177 DOI: 10.1007/s10695-011-9588-7

Abstract

Tor tor is an important game and food fish of India with a distribution throughout Asia from the trans-Himalayan region to the Mekong River basin to Malaysia, Pakistan, Bangladesh and Indonesia. A new cell line named TTCF was developed from the caudal fin of T. tor for the first time. The cell line was optimally maintained at 28°C in Leibovitz-15 (L-15) medium supplemented with 20% fetal bovine serum (FBS). The propagation of TTCF cells showed a high plating efficiency of 63.00%. The cytogenetic analysis revealed a diploid count of 100 chromosomes at passage 15, 30, 45 and 60 passages. The viability of the TTCF cell line was found to be 72% after 6 months of cryopreservation in liquid nitrogen (-196°C). The origin of the cell lines was confirmed by the amplification of 578- and 655-bp sequences of 16S rRNA and cytochrome oxidase subunit I (COI) genes of mitochondrial DNA (mtDNA) respectively. TTCF cells were successfully transfected with green fluorescent protein (GFP) reporter plasmids. Further, immunocytochemistry studies confirm its fibroblastic morphology of cells. Genotoxicity assessment of H₂O₂ in TTCF cell line revealed the utility of TTCF cell line as in vitro model for aquatic toxicological studies.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.