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Clausenidin induces caspase-dependent apoptosis in colon cancer

Waziri PM 1 , Abdullah R 2 , Yeap SK 3 , Omar AR 3 , Kassim NK 4 , Malami I 5 Show all authors , How CW 3 , Etti IC 5 , Abu ML 6

Affiliations 

  • 1 MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia. petermwaziri@gmail.com
  • 2 Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 3 Laboratory of Vaccine and Therapeutics, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 4 Department of Chemistry, Faculty of Science, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 5 MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia
  • 6 Enzyme Technology Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia
BMC Complement Altern Med, 2016 Jul 29;16:256.
PMID: 27473055 DOI: 10.1186/s12906-016-1247-1

Abstract

BACKGROUND: Clausena excavata Burm.f. is a shrub traditionally used to treat cancer patients in Asia. The main bioactive chemical components of the plant are alkaloids and coumarins. In this study, we isolated clausenidin from the roots of C. excavata to determine its apoptotic effect on the colon cancer (HT-29) cell line.
METHOD: We examined the effect of clausenidin on cell viability, ROS generation, DNA fragmentation, mitochondrial membrane potential in HT-29 cells. Ultrastructural analysis was conducted for morphological evidence of apoptosis in the treated HT-29 cells. In addition, we also evaluated the effect of clausenidin treatment on the expression of caspase 3 and 9 genes and proteins in HT-29 cells.
RESULT: Clausenidin induced a G0/G1 cell cycle arrest in HT-29 cells with significant (p 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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