Globally, the spread of HIV/AIDS is a major public health challenge because of its pandemic magnitudes. Despite the fact that people of any age are susceptible to HIV, the younger population is more susceptible to be infected because of their lifestyle choices. The purpose of this study was to assess the extent of HIV/AIDS knowledge level among health science undergraduate university students as they play a primary role within the health care system of a country. A cross-sectional study was conducted by using a semi structured, self-administered, especially design questionnaire. Out of total one hundred fifty undergraduate university students, there were 96 males (64%) and 54 females (36%) with the age ranging from 19-25 years. All the students were able to write the acronym of HIV (100%) and AIDS (100%). Common routes of transmission and prevention measure were known by majority of the students. Students stated correctly regarding HIV/AIDS transmission from mother to child through pregnancy (18.02%), delivery (15.31%) and breast feeding (11.30%) which was very low level of knowledge. Most of the medical, dental and physiotherapy students had correct knowledge on high risk populace for HIV/AIDS; like singles, intravenous drug user, multiple sex partners and commercial sexual partner. Regarding the preventive measure as hand washing, use of facial mask and sexual abstinent was correctly mentioned by only 31.34%, 34.02% and 28.01% total students respectively. However other safety measure like condom use, effectiveness of condom use, screening of blood was widely known by the students. However, regarding the knowledge on treatment issue of HIV/AIDS, majority of the students (85.30%) correctly knew that HIV/AIDS was not curable, whereas only 15.31% and 20.01% correctly knew that the treatment was very expensive and available respectively. So, this study focus the educational endeavors that could be broadened to emphasis on its general knowledge on HIV/AIDS.