Affiliations 

  • 1 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia; Centre for Water Research and Analysis (ALIR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia. Electronic address: farina@ukm.edu.my
  • 2 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia; Centre for Water Research and Analysis (ALIR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
  • 3 School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia; Centre for Water Research and Analysis (ALIR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia; Department of Chemistry, Faculty of Science, Sardar Bahadur Khan Women University, Quetta, Pakistan
  • 4 Centre for Water Research and Analysis (ALIR), Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
Food Chem, 2017 Jun 01;224:55-61.
PMID: 28159293 DOI: 10.1016/j.foodchem.2016.11.113

Abstract

A simple and sensitive analytical method has been developed employing gas chromatography coupled with electron capture detector (GC-ECD), and validated for screening and quantification of 15 pesticide residues at trace levels in cabbage, broccoli, cauliflower, lettuce, celery, spinach, and mustard. The method consists of two steps, first, to determine the significance of each factor by Pareto chart followed by optimization of these significant factors using central composite design (CCD). Minitab statistical software was used for these multivariate experiments for the generation of 2(4-1) design and CCD matrices. The method evaluation was done by external standard calibration with linearity range between 0.5 and 3mg/kg, with correlation coefficient 0.99, limit of detection (LOD) ranges between 0.02 and 4.5ng/g, and limit of quantification (LOQ) ranges between 0.2 and 45ng/g. The average recovery was between 60% and 128%, with RSD 0.2-19.8%. The method was applied on real vegetable samples from Cameron Highlands.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.