Levels of physical activity are usually affected by a high motivational factor. There are many internal and external motivational factors. Less physical activity or a sedentary lifestyle can be defined as movement of the body equal to the minimum rest metabolic rate. It also refers to passive physical behavior such as watching television, reading, using a computer, talking on the phone, driving a car, meditating, or eating. This study was conducted to determine and investigate the motivational factors that influence participation in sports and physical activities. A survey questionnaire based on the "Exercise Motivation Inventory" (EMI-2) and the "International Physical Activity Questionnaire" (IPAQ) was developed to meet the needs of the study. This study looked at the relationship between motivational factors and the level of physical activity and sports participation among university staff, who involved in their daily lives. Findings that showed university staff were more motivated to perform moderate physical activity in daily life. The motives for physical activity are both in terms of appearance and social factors. Inferential analysis indicated no significant difference in terms of the motives for employee engagement in physical activity and status based on weight. Correlation analysis showed a significant relationship between physical activity and body weight among staff. There was no significant relationship between participation motives and physical activity among employees.