Determination of alpha-2-MRAP gene polymorphisms in nephrolithiasis patients

Mehde AA 1 , Mehdi WA 2 , Yusof F 3 , Raus RA 3 , Abidin ZAZ 4 , Ghazali H 5 Show all authors , Rahman AA 6

Affiliations 

  • 1 Biomedical, Magnetic and Semi Conductive Materials Application and Research Centre, Sakarya University, Sakarya, Turkey; Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia
  • 2 Biomedical, Magnetic and Semi Conductive Materials Application and Research Centre, Sakarya University, Sakarya, Turkey. Electronic address: wesenm@sakarya.edu.tr
  • 3 Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia
  • 4 Department of Biotechnology, Faculty of Science, International Islamic University Malaysia, Kuantan 25200, Malaysia
  • 5 Department of Urology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia
  • 6 Department of Pathology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia
Int J Biol Macromol, 2017 Dec;105(Pt 1):1324-1327.
PMID: 28760704 DOI: 10.1016/j.ijbiomac.2017.07.167

Abstract

BACKGROUND: The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients.

METHODS: PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined.

RESULTS: The D and I allele frequencies were 57.50% and 42.50% in patients, 77.50% and 22.50% in control, individually. The result showed that II genotype was associated with nephrolithiasis patients group. A significant decrease was observed in serum Alpha-2-MRAP,myeloperoxidase and TAS,while TOS,OSI,MDA,AOPP and uric acid were substantially increased in II and ID when compared to DD genotype in patients with nephrolithiasis.

CONCLUSION: Our results demonstrate for the first time that patients with II genotype had an increased risk of stones. Also, the results demonstrate that I allele of the 5 insertion/deletion polymorphism in the Alpha-2-MRAP gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with II genotype of Alpha-2-MRAP.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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