Acanthamoebicidal activity of periglaucine A and betulinic acid from Pericampylus glaucus (Lam.) Merr. in vitro

Mahboob T 1 , Azlan AM 1 , Shipton FN 2 , Boonroumkaew P 1 , Nor Azman NS 2 , Sekaran SD 3 Show all authors , Ithoi I 1 , Tan TC 1 , Samudi C 3 , Wiart C 4 , Nissapatorn V 5

Affiliations 

  • 1 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 2 School of Pharmacy, Faculty of Science, University of Nottingham Malaysia Campus, Semenyih, 43500 Selangor, Malaysia
  • 3 Department of Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 4 School of Pharmacy, Faculty of Science, University of Nottingham Malaysia Campus, Semenyih, 43500 Selangor, Malaysia. Electronic address: Christophe.Wiart@nottingham.edu.my
  • 5 Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia; School of Allied Health Sciences, Walailak University, 80161 Nakhon Si Thammarat, Thailand. Electronic address: nissapat@gmail.com
Exp Parasitol, 2017 Dec;183:160-166.
PMID: 28916456 DOI: 10.1016/j.exppara.2017.09.002

Abstract

Acanthamoeba species are pathogenic protozoa which account for amoebic keratitis, conjunctivitis and granulomatous amoebic encephalitis. These amoebae form cysts which resist drugs and more effective acanthamoebicidal agents are needed. Medicinal plants could be useful in improving the current treatment strategies for Acanthamoeba infections. In the present study, we examined the amoebicidal effects of Pericampylus glaucus (Lam.) Merr., a medicinal plant used for the treatment of conjunctivitis in Malaysia. Pathogenic Acanthamoeba triangularis were isolated from environmental water samples and treated with different concentrations of fractions obtained from Pericampylus glaucus (Lam.) Merr. as well as main constituents for 24-72 h. Chlorhexidine was used as a reference drug. Ethanol fraction of stem showed significant (p 

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