Affiliations 

  • 1 Vascular Biology Laboratory, AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai 600 044, India. Electronic address: vimalr50@gmail.com
  • 2 Chemical Biology and Nanobiotechnology Laboratory, AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai 600 044, India. Electronic address: rajimaniyam@gmail.com
  • 3 Chemical Biology and Nanobiotechnology Laboratory, AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai 600 044, India
  • 4 Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Tamil Nadu, India
  • 5 Helicobacter Research Laboratory, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
  • 6 Thiruvalluvar University (State University), Serkkadu, Vellore 632 115, Tamil Nadu, India
  • 7 Vascular Biology Laboratory, AU-KBC Research Centre, Anna University, MIT Campus, Chromepet, Chennai 600 044, India
Mater Sci Eng C Mater Biol Appl, 2018 Feb 01;83:187-194.
PMID: 29208278 DOI: 10.1016/j.msec.2017.09.005

Abstract

Copper(II) complex of quercetin Cu+Q, mixed ligand complexes, quercetin-Cu(II)-phenanthroline [Cu+Q(PHt)] and quercetin-Cu(II)-neocuproine [Cu+Q(Neo)] have been synthesized and characterized. From the FT-IR spectroscopic studies, it was evident that C-ring of quercetin is involved in the metal chelation in all the three copper complexes. C-ring chelation was further proven by UV-Visible spectra and the presence of Cu(II) from EPR spectroscopic investigations. These complexes were found to have osteogenic and angiogenic properties, observed through in vitro osteoblast differentiation and chick embryo angiogenesis assay. In osteoblast differentiation, quercetin-Cu(II) complexes treatment increased calcium deposition and alkaline phosphatase activity (ALP) activity at the cellular level and stimulated Runx2 mRNA and protein, ALP mRNA and type 1 collagen mRNA expression at the molecular level. Among the complexes, Q+Cu(PHt) showed more effects on osteoblast differentiation when compared to that of other two copper complexes. Additionally, Q+Cu(Neo) showed more effect compared to Q+Cu. Furthermore, the effect of these complexes on osteoblast differentiation was confirmed by the expression of osteoblast specific microRNA, pre-mir-15b. The chick embryo angiogenesis assay showed that angiogenic parameters such as blood vessel length, size and junctions were stimulated by these complexes. Thus, the present study demonstrated that quercetin copper(II) complexes exhibit as a pharmacological agent for the orthopedic application.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.