Knockout of CTNNB1 by CRISPR-Cas9 technology inhibits cell proliferation through the Wnt/β-catenin signaling pathway

Guan L 1 , Zhu S 1 , Han Y 2 , Yang C 1 , Liu Y 1 , Qiao L 1 Show all authors , Li X 1 , Li H 1 , Lin J 3

Affiliations 

  • 1 College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China
  • 2 College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, China
  • 3 College of Life Science and Technology, Xinxiang Medical University, Xinxiang, China. linjtlin@126.com
Biotechnol Lett, 2018 Mar;40(3):501-508.
PMID: 29249062 DOI: 10.1007/s10529-017-2491-2

Abstract

OBJECTIVE: To study the effects of CTNNB1 gene knockout by CRISPR-Cas9 technology on cell adhesion, proliferation, apoptosis, and Wnt/β-catenin signaling pathway.

RESULTS: CTNNB1 gene of HEK 293T cells was knocked out by CRISPR-Cas9. This was confirmed by sequencing and western blotting. Methylthiazolyl-tetrazolium bromide assays indicated that deletion of β-catenin significantly weakened adhesion ability and inhibited proliferation rate (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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