Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY

Wong YM 1 , Wu TY 2 , Ling TC 3 , Show PL 4 , Lee SY 4 , Chang JS 5 Show all authors , Ibrahim S 6 , Juan JC 7

Affiliations 

  • 1 Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia
  • 2 Chemical and Sustainable Process Engineering Research Group, School of Engineering, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia
  • 3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 4 Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia
  • 5 Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan
  • 6 Department of Civil Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia
  • 7 Laboratory of Advance Catalysis and Environmental Technology, School of Science, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Malaysia; Nanotechnology and Catalysis Research Centre (NANOCAT), University of Malaya, 50603 Kuala Lumpur, Malaysia. Electronic address: jcjuan@um.edu.my
J Biosci Bioeng, 2018 May;125(5):590-598.
PMID: 29352712 DOI: 10.1016/j.jbiosc.2017.12.012

Abstract

Three newly discovered H2 producing bacteria namely Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY originated from landfill leachate sludge have demonstrated highly efficient H2 production. The maximum H2 production attained from these isolates are in the descending order of strain C. perfringens strain JJC > C. bifermentans strain WYM > Clostridium sp. strain Ade.TY with yield of 4.68 ± 0.12, 3.29 ± 0.11, and 2.87 ± 0.10 mol H2/mol glucose, respectively. The result has broken the conventional theoretical yield of 4 mol H2/mol glucose. These isolates were thermodynamically favourable with Gibbs free energy between -33 and -35 kJ/mol (under process conditions: pH 6, 37 °C and 5 g/L glucose). All three isolates favour butyrate pathway for H2 production with the ratio of acetate and butyrate of 0.77, 0.65 and 0.80 for strain JJC, WYM and Ade.TY, respectively. This study reported provides a new insight on the potential of unique bacteria in H2 production.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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