Photodynamic inactivation of Burkholderia cepacia by curcumin in combination with EDTA

Hu J 1 , Lin S 1 , Tan BK 2 , Hamzah SS 3 , Lin Y 1 , Kong Z 1 Show all authors , Zhang Y 1 , Zheng B 4 , Zeng S 5

Affiliations 

  • 1 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • 2 Departments of Cardiovascular Sciences, Health Sciences and Leicester Diabetes Centre, College of Life Sciences, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom; Translational and Experimental Medicine, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry CV4 7AL, United Kingdom
  • 3 Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia
  • 4 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: zbdfst@163.com
  • 5 College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; China-Ireland International Cooperation Centre for Food Material Science and Structure Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: zsxfst@163.com
Food Res Int, 2018 09;111:265-271.
PMID: 30007685 DOI: 10.1016/j.foodres.2018.05.042

Abstract

Burkholderia cepacia (B. cepacia) is an aerobic Gram-negative bacillus found in various aquatic environments and can cause food contamination. We investigated the photodynamic antibacterial effects of food additive curcumin combined with EDTA on B. cepacia. We found a ~4-log reduction in B. cepacia viability when photo-irradiated with curcumin at 50 μM by blue LED light (16 mW/cm2) for 30 min with 0.4% (w/v) EDTA. Moreover, the bacterial morphological alterations and the leakage of intracellular contents were observed after photodynamic treatment. There were also obvious genomic DNA cleavage and a general loss of bacterial proteins assigned to large-scale protein degradation after photodynamic inactivation treatment. Collectively, curcumin in combination with EDTA illuminated by blue LED is a potential candidate for photodynamic inactivation of B. cepacia.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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