Affiliations 

  • 1 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. umizulaikha.ika@gmail.com
  • 2 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. azahy@upm.edu.my
  • 3 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. norzilakusnin87@gmail.com
  • 4 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. jafar@upm.edu.my
  • 5 School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia. ssuraiya@usm.my
  • 6 NanoMalaysia Berhad, a CLG under the Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Kuala Lumpur 50450, Malaysia. pohshing.ong@nanomalaysia.com.my
  • 7 School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM Bangi 43600, Selangor, Malaysia. nurulhanun@ukm.edu.my
  • 8 Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. siti_fatimahar@upm.edu.my
  • 9 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar 01000, Perlis, Malaysia. mohamadfaris@unimap.edu.my
Sensors (Basel), 2018 Nov 14;18(11).
PMID: 30441776 DOI: 10.3390/s18113926

Abstract

A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of tuberculosis biomarker 10-kDa culture filtrate protein (CFP10). The prepared GP/PANI nanocomposite was characterized using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM). The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and FESEM coupled with energy dispersive X-ray spectroscopy, respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced about five times compared with bare SPGE. Differential pulse voltammetry was used to detect the CFP10 antigen. The GP/PANI-modified SPGE that was fortified with sandwich type immunosensor exhibited a wide linear range (20⁻100 ng/mL) with a low detection limit of 15 ng/mL. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid and disposable, which is suitable for tuberculosis detection in real samples.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.