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Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan

Ali MS 1 , Isa NM 2 , Abedelrhman FM 1 , Alyas TB 3 , Mohammed SE 4 , Ahmed AE 1 Show all authors , Ahmed ZSA 1 , Lau NS 5 , Garbi MI 6 , Amirul AA 5 , Seed AO 1 , Omer RA 1 , Mohamed SB 7

Affiliations 

  • 1 Department of Bioinformatics and Biostatistics, National University Research Institute- National University, Khartoum, Sudan
  • 2 Department of Cell and Molecular Biology, Faculty of Biotechnology and Bimolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia
  • 3 Tropical Medicine Research Institute, Khartoum, Sudan
  • 4 Department of microbiology, faculty of medical laboratory sciences, National University, Khartoum, Sudan
  • 5 Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Bayan Lepas, Penang, Malaysia
  • 6 Department of Microbiology, Faculty of Medical Laboratory Science, International University of Africa, Khartoum, Sudan
  • 7 Department of Bioinformatics and Biostatistics, National University Research Institute- National University, Khartoum, Sudan. Sofiabashir2002@gmail.com
BMC Microbiol, 2019 06 11;19(1):126.
PMID: 31185900 DOI: 10.1186/s12866-019-1470-2

Abstract

BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnostic assays. The objective of the present study is to perform whole-genome sequencing of MRSA isolated from Sudan using Illumina Next Generation Sequencing (NGS) platform.

RESULTS: The genome of MRSA strain SO-1977 consists of 2,827,644 bp with 32.8% G + C, 59 RNAs and 2629 predicted coding sequences (CDSs). The genome has 26 systems, one of which is the major class in the disease virulence and defence. A total of 83 genes were annotated to virulence disease and defence category some of these genes coding as functional proteins. Based on genome analysis, it is speculated that the SO-1977 strain has resistant genes to Teicoplanin, Fluoroquinolones, Quinolone, Cephamycins, Tetracycline, Acriflavin and Carbapenems. The results revealed that the SO-1977, strain isolated from Sudan has a wide range of antibiotic resistance compared to related strains.

CONCLUSION: The study reports for the first time the whole genome sequence of Sudan MRSA isolates. The release of the genome sequence of the strain SO-1977 will avail MRSA in public databases for further investigations on the evolution of resistant mechanism and dissemination of the -resistant genes of MRSA.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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