Aptamer-antibody dual probes on single-walled carbon nanotube bridged dielectrode: Comparative analysis on human blood clotting factor

Yao J 1 , Li S 2 , Zhang L 3 , Yang Y 3 , Gopinath SCB 4 , Lakshmipriya T 5 Show all authors , Zhou Y 6

Affiliations 

  • 1 Emergency Department, The Affiliated Hospital of Qingdao University, Qingdao 266000, PR China
  • 2 Department of Radiology, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province 250013, PR China
  • 3 Department of Hematology, Dezhou People's Hospital, 1751 Xinhu Road, Dezhou, Shandong Province 253014, PR China
  • 4 School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Malaysia; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
  • 5 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
  • 6 Department of Hematology, Jinan Central Hospital Affiliated to Shandong University, No. 105, Jiefang Road, Lixia District, Jinan, Shandong Province 250013, PR China. Electronic address: zywhizxyy@sina.com
Int J Biol Macromol, 2020 May 15;151:1133-1138.
PMID: 31743722 DOI: 10.1016/j.ijbiomac.2019.10.156

Abstract

Haemophilia is a blood clotting disorder known as 'Christmas disease' caused when the blood has defect with the clotting factor(s). Bleeding leads various issues, such as chronic pain, arthritis and a serious complication during the surgery. Identifying this disease is mandatory to take the necessary treatment and maintains the normal clotting. It has been proved that the level of factor IX (FIX) is lesser with haemophilia patient and the attempt here is focused to quantify FIX level by interdigitated electrode (IDE) sensor. Single-walled carbon nanotube (SWCNT) was utilized to modify IDE sensing surface. On this surface, dual probing was evaluated with aptamer and antibody to bring the possible advantages. The detection limit with antibody was found to be 1 pM, while aptamer shows 100 fM. Further, a fine-tuning was attempted with sandwich pattern of aptamer-FIX-antibody and antibody-FIX-aptamer and compared. Specific elevation of detection with 10 folds was noticed and displayed the detection at 100 f. in both sandwich patterns. In addition, FIX was detected in the diluted human serum by aptamer-FIX-antibody sandwich, it was found that FIX detected from the dilution factor 1:640. A novel demonstration is with higher discrimination against other clotting factors, XI and VII.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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