Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Gu Y 1 , Liu L 2 , Guo J 3 , Xiao S 4 , Fang F 5 , Yu X 5 Show all authors , Gopinath SCB 6 , Wu J 5 , Liu X 7

Affiliations 

  • 1 Department of Vascular Surgery, Tianjin First Center Hospital, Tianjin, China
  • 2 Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao City, China
  • 3 Department of Imaging, The Affiliated Hospital of Qingdao University, Qingdao City, China
  • 4 Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao City, China
  • 5 Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao City, China
  • 6 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Perlis, Malaysia
  • 7 Department of Vascular surgery, Yan'an Hospital Affiliated to Kunming Medical University, Kunming City, Yunnan Province, China
Artif Cells Nanomed Biotechnol, 2021 Dec;49(1):30-37.
PMID: 33467925 DOI: 10.1080/21691401.2020.1865992

Abstract

This research is focussed to quantify IGF1 by electroanalytical analysis on InterDigitated electrode surface and characterized by the microscopic observations. For the detection, antibody and aptamer were used to analyze the level of IGF1. The sandwich pattern (aptamer-IGF1-antibody) was designed on the chemically modified IDE surface and reached the limit of detection to 10 fM with 100 folds enhancement in the sensitivity. Different control experiments (absence of IGF1, binding with IGF2 and with non-complementary aptamer) were failed to show the current changes, discriminated the specific detection. A good detection strategy is to complement the currently following imaging systems for AAA.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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