Affiliations 

  • 1 Department of Anesthesiology, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, Shaanxi, 712000, People's Republic of China
  • 2 Department of Anesthesiology, Xi'an GemFlower Changqing Hospital, Xi'an, Shaanxi, 710200, People's Republic of China
  • 3 The Third Department of Neurology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710038, People's Republic of China
  • 4 Department of Neurology, The Hospital of Xidian Group, Xi'an, Shaanxi, 710077, People's Republic of China
  • 5 Department of Otolaryngology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710038, People's Republic of China
  • 6 Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, Shaanxi, 710038, People's Republic of China
  • 7 Faculty of Chemical Engineering Technology, Universiti Malaysia Perlis (UniMAP), Arau, Perlis, 02600, Malaysia
  • 8 Department of Biological Engineering, College of Engineering, Inha University, Incheon, 402-751, Republic of Korea
  • 9 Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), Kangar, 01000, Malaysia
  • 10 Department of Surgery and Anesthesiology, Xi'an Hospital of Traditional Chinese Medicine, Xi'an, Shaanxi, People's Republic of China
Int J Nanomedicine, 2021;16:2311-2322.
PMID: 33776435 DOI: 10.2147/IJN.S302396

Abstract

Background: Alzheimer's disease (AD) is a neurodegenerative chronic disorder that causes dementia and problems in thinking, cognitive impairment and behavioral changes. Amyloid-beta (Aβ) is a peptide involved in AD progression, and a high level of Aβ is highly correlated with severe AD. Identifying and quantifying Aβ levels helps in the early treatment of AD and reduces the factors associated with AD.

Materials and Methods: This research introduced a dual probe detection system involving aptamers and antibodies to identify Aβ. Aptamers and antibodies were attached to the gold (Au) urchin and hybrid on the carbon nanohorn-modified surface. The nanohorn was immobilized on the sensor surface by using an amine linker, and then a Au urchin dual probe was immobilized.

Results: This dual probe-modified surface enhanced the current flow during Aβ detection compared with the surface with antibody as the probe. This dual probe interacted with higher numbers of Aβ peptides and reached the detection limit at 10 fM with R2=0.992. Furthermore, control experiments with nonimmune antibodies, complementary aptamer sequences and control proteins did not display the current responses, indicating the specific detection of Aβ.

Conclusion: Aβ-spiked artificial cerebrospinal fluid showed a similar response to current changes, confirming the selective identification of Aβ.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.