Affiliations 

  • 1 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Consortium of Malaysia IPT Halal Institutes, Ministry of Higher Education, Complex E, Federal Government Administrative Centre, 62604 Putrajaya, Malaysia. Electronic address: hafisyuswan@upm.edu.my
  • 2 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • 3 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Consortium of Malaysia IPT Halal Institutes, Ministry of Higher Education, Complex E, Federal Government Administrative Centre, 62604 Putrajaya, Malaysia
  • 4 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Consortium of Malaysia IPT Halal Institutes, Ministry of Higher Education, Complex E, Federal Government Administrative Centre, 62604 Putrajaya, Malaysia
  • 5 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Consortium of Malaysia IPT Halal Institutes, Ministry of Higher Education, Complex E, Federal Government Administrative Centre, 62604 Putrajaya, Malaysia
  • 6 Laboratory of Halal Services, Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Consortium of Malaysia IPT Halal Institutes, Ministry of Higher Education, Complex E, Federal Government Administrative Centre, 62604 Putrajaya, Malaysia
Food Chem, 2021 Feb 01;337:127762.
PMID: 32777563 DOI: 10.1016/j.foodchem.2020.127762

Abstract

Gelatin and collagen are considered halal-critical ingredients as they are typically derived from either bovine or porcine animals. Current analytical methods for determining the sources of gelatin and collagen suffer from limitations in terms of robustness and false positives in peptide matching. Thus, the aim of this study was to investigate the utility of monitoring hydroxyproline, a signature amino acid for gelatin and collagen, for identifying potentially haram foodstuffs. To determine the hydroxyproline profiles among animal- and plant-based samples, one-way univariate analysis of variance followed by pair-wise comparison was used to establish statistical significance. Multivariate chemometric analysis through principal component analysis revealed a discrete distribution pattern among 59 samples due to hydroxyproline variability. Finally, inter- and intra-laboratory comparisons demonstrated the validity and robustness of hydroxyproline determination according to ISO 17025. Thus, this preliminary identification technique will aid the identification of potentially haram foodstuffs.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.