Introduction: Collagen and gelatin are essential protein in vertebrates and extensively used in various industries. Methods: In this study, acid-solubilized collagen and gelatin were extracted from the scales of three different species of freshwater fish namely Kelah (Tombroides), Tilapia (Oreochromis niloticus) and Snakehead fish (Channidae) and then further quantified using Bradford assay and separated by molecular weight using SDS-PAGE. Results: The extracted collagen in Tilapia fish scale was found to be the highest with 0.018 of protein absorbance among the other three fish; Kelah fish (0.017) and Snakehead fish (0.011). For gelatin, Snakehead fish scales showed the highest amount of total protein concentration followed by Tilapia and Kelah fish with 0.467, 0.144 and 0.037 μg/μL per g, respectively. Based on the SDS-PAGE results, collagen from all the three freshwater fishes were identified as a type 1 (molecular weight approximately from 95 to 130 kDa) collagen. As for gelatin, only gelatin from Snakehead fish scale was identified to be a type 1(molecular weight approximately from 95 to 130 kDa) while the other two freshwater fishes showed no clear band due to high viscosity of the gelatin produced. Conclusion: It can be said that the fishes investigated in this study have a potential to be the alternative source of collagen and gelatin.