Affiliations 

  • 1 Collaborative Microelectronic Design Excellence Centre (CEDEC), Sains@USM, Universiti Sains Malaysia, Level 1, Block C, No. 10 Persiaran Bukit Jambul, Bayan Lepas 11900, Malaysia
  • 2 School of Biological Sciences, Universiti Sains Malaysia (USM), Gelugor 11800, Malaysia
  • 3 School of Chemical Sciences, Universiti Sains Malaysia (USM), Gelugor 11800, Malaysia
  • 4 Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia (USM), Kubang Kerian 16150, Malaysia
Biosensors (Basel), 2021 Sep 18;11(9).
PMID: 34562936 DOI: 10.3390/bios11090346

Abstract

Large-scale food-borne outbreaks caused by Salmonella are rarely seen nowadays, thanks to the advanced nature of the medical system. However, small, localised outbreaks in certain regions still exist and could possess a huge threat to the public health if eradication measure is not initiated. This review discusses the progress of Salmonella detection approaches covering their basic principles, characteristics, applications, and performances. Conventional Salmonella detection is usually performed using a culture-based method, which is time-consuming, labour intensive, and unsuitable for on-site testing and high-throughput analysis. To date, there are many detection methods with a unique detection system available for Salmonella detection utilising immunological-based techniques, molecular-based techniques, mass spectrometry, spectroscopy, optical phenotyping, and biosensor methods. The electrochemical biosensor has growing interest in Salmonella detection mainly due to its excellent sensitivity, rapidity, and portability. The use of a highly specific bioreceptor, such as aptamers, and the application of nanomaterials are contributing factors to these excellent characteristics. Furthermore, insight on the types of biorecognition elements, the principles of electrochemical transduction elements, and the miniaturisation potential of electrochemical biosensors are discussed.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.