• 1 a School of Life Sciences, Northwestern Polytechnical University , Xi'an , P.R. China
  • 2 c The Key Laboratory of Biomedical Information Engineering of Ministry of Education , School of Life Science and Technology, Xi'an Jiaotong University , Xi'an , P.R. China
  • 3 e Department of Biomedical Engineering, Faculty of Engineering , University of Malaya , Lembah Pantai , Kuala Lumpur , Malaysia
Crit Rev Biotechnol, 2016 Apr 14.
PMID: 27075621 DOI: 10.3109/07388551.2016.1164664


In recent years, paper-based point-of-care testing (POCT) has been widely used in medical diagnostics, food safety and environmental monitoring. However, a high-cost, time-consuming and equipment-dependent sample pretreatment technique is generally required for raw sample processing, which are impractical for low-resource and disease-endemic areas. Therefore, there is an escalating demand for a cost-effective, simple and portable pretreatment technique, to be coupled with the commonly used paper-based assay (e.g. lateral flow assay) in POCT. In this review, we focus on the importance of using paper as a platform for sample pretreatment. We firstly discuss the beneficial use of paper for sample pretreatment, including sample collection and storage, separation, extraction, and concentration. We highlight the working principle and fabrication of each sample pretreatment device, the existing challenges and the future perspectives for developing paper-based sample pretreatment technique.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.