Displaying publications 1 - 20 of 32 in total

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  1. Zzaman W, Bhat R, Yang TA, Easa AM
    J Sci Food Agric, 2017 Oct;97(13):4429-4437.
    PMID: 28251656 DOI: 10.1002/jsfa.8302
    BACKGROUND: Roasting is one of the important unit operations in the cocoa-based industries in order to develop unique flavour in products. Cocoa beans were subjected to roasting at different temperatures and times using superheated steam. The influence of roasting temperature (150-250°C) and time (10-50 min) on sugars, free amino acids and volatile flavouring compounds were investigated.

    RESULTS: The concentration of total reducing sugars was reduced by up to 64.61, 77.22 and 82.52% with increased roasting temperature at 150, 200 and 250°C for 50 min, respectively. The hydrophobic amino acids were reduced up to 29.21, 36.41 and 48.87% with increased roasting temperature at 150, 200 and 250°C for 50 min, respectively. A number of pyrazines, esters, aldehydes, alcohols, ketones, carboxyl acids and hydrocarbons were detected in all the samples at different concentration range. Formation of the most flavour active compounds, pyrazines, were the highest concentration (2.96 mg kg-1 ) at 200°C for 10 min.

    CONCLUSION: The superheated steam roasting method achieves the optimum roasting condition within a short duration Therefore, the quality of cocoa beans can be improved using superheated steam during the roasting process. © 2017 Society of Chemical Industry.

  2. Bhat R, Goh KM
    Food Chem, 2017 Jan 15;215:470-6.
    PMID: 27542500 DOI: 10.1016/j.foodchem.2016.07.160
    Hand-pressed strawberry juice samples were subjected to sonication treatments (0, 15 and 30min at 20°C, 25kHz frequency). Physicochemical properties (°Brix, pH, water activity, viscosity, titratable acidity, cloud assessment and turbidity), antioxidant compounds and activity (total phenolics, ascorbic acid, anthocyanins, free radical scavenging activity), polyphenoloxidase enzyme activity, browning degree and microbial load were evaluated. Results showed non-significant changes for °Brix, pH, water activity, titratable acidity and colour parameters in sonicated samples compared to control (0min). Sonication treatments resulted in reduced viscosity and increased cloudiness and turbidity. Overall, treatment for 30min showed significant enhancement in bioactive compounds under study. Besides, sonication treatment imparted non-significant changes in polyphenoloxidase activity and in browning degree. However, sonication was incompetent in reducing microbial load. Results generated from this study were encouraging and this is expected to provide platform for future commercial applications on a pilot scale.
  3. Bhat, Rajeev, Nurul Hanida Ismail, Yeoh, T.K.
    MyJurnal
    Goat milk is a highly nutritious and an ideal wholesome food. Today, an increase in demand by
    consumers is witnessed towards consuming goat milk, mainly owed to their rich nutraceutical
    value. In this study, novel goat milk bar was developed by incorporating rose flower extracts
    as an added ingredient (concentration level: 0, 5, 10, 15 and 20%), and by using two different
    types of natural sweeteners (cane sugar and palm sugar). The nutrition bars were evaluated
    for proximate composition, texture properties and sensory qualities. Results obtained were
    encouraging and the new goat milk based nutritional bar formulation with added rose extracts
    certainly paves way for future commercial exploitation of the product. Goat milk, owing to its
    rich nutraceutical value, and rose extracts owing to the dual functions of a natural antioxidant
    and antimicrobial agent, can be beneficial for extending the shelf life of this novel product
    under room or refrigerated temperatures, thus attracting better markets.
  4. Bhat R, Stamminger R
    Food Sci Technol Int, 2015 Jul;21(5):354-63.
    PMID: 24867944 DOI: 10.1177/1082013214536708
    Freshly prepared, hand-pressed strawberry fruit juice was exposed to ultraviolet radiation (254 nm) at room temperature (25 ℃ ± 1 ℃) for 15, 30 and 60 min with 0 min serving as control. Results revealed decrease in pH, total soluble solids and titratable acidity, while colour parameters (L*, a* and b* values) and clarity of juice (% transmittance) increased significantly. All the results corresponded to exposure time to ultraviolet radiation. Bioactive compounds (total phenolics, ascorbic acid and anthocyanins) decreased along with a recorded reduction in polyphenol oxidase enzyme and 1,1-diphenyl-2-picryl hydrazyl radical scavenging activities, which were again dependent on exposure time. Results on the microbial studies showed significant reduction by 2-log cycles in aerobic plate count as well as in total yeast and mould counts. Though negative results were observed for certain parameters, this is the first time it was endeavoured to demonstrate the impact of ultraviolet radiation radiation on freshly prepared, hand-pressed strawberries juice.
  5. Ho LH, Bhat R
    Food Chem, 2015 Feb 1;168:80-9.
    PMID: 25172686 DOI: 10.1016/j.foodchem.2014.07.020
    This review focuses on providing informations on potential uses of durian, an exotic tropical fruit as a source of food, as well as a potential therapeutic agent. Apart from disseminating details on the traditional value, in this review we have focussed on the nutritional composition, presence of bioactive compounds, volatiles, antimicrobials, as well as on the toxicological effects of durian fruit consumption. Durian fruits are enjoyed for their unique taste and organoleptic qualities, but there is also a need to ensure that their potential is exploited for the international market. In addition, in the present socio-economic scenario, tapping the potential of exotic tropical fruit such as durian could benefit the health of consumers as well as support the local population who depend on farming for a livelihood. Overall, it is envisaged that identifying the nutraceutical potential of the edible and non-edible parts of durian fruits can benefit food and pharmaceutical industries.
  6. Thomas, R., Bhat, Rajeev, Kuang, Y.T.
    MyJurnal
    In this study, three popular, regionally grown rice varieties (Bario, brown and white) were compared with three of the most popular and highly marketed imported rice varieties (black, glutinous and basmati rice) in Penang region of Malaysia. Rice samples were evaluated for amino acids, fatty acids, minerals, heavy metals and dietary fiber composition. Overall, amino acids content among all the rice samples were comparable to each other. Results with regard to minerals showed potassium to be high in brown rice (197.41 mg/100g), while magnesium was recorded to be high in black rice (107.21 mg/100g). Heavy metals such as cadmium, nickel, mercury and lead, though present, they were in negligible amounts. Among all the rice varieties investigated, the total saturated fatty acid and unsaturated fatty acid content was highest in black rice (5.89%). The soluble dietary fiber was higher in white rice (16.39%), whereas insoluble dietary fiber was high in brown (16.51%) and black rice (14.49%), respectively. Results generated from this study is anticipated to benefit both the health wary consumers (based on their potential nutritional attributes) as well as the local food industries to choose the best rice variety while developing novel rice based food products.
  7. Bhat R, binti Yahya N
    Food Chem, 2014 Aug 1;156:42-9.
    PMID: 24629936 DOI: 10.1016/j.foodchem.2014.01.063
    Belinjau (Gnetum gnemon L.) seed flour was evaluated for nutritional composition, antioxidant activity and functional properties. Seed flour was found to be rich in protein (19.0g/100g), crude fibre (8.66g/100g), carbohydrates (64.1%), total dietary fibre (14.5%) and encompassed adequate amounts of essential amino acids, fatty acids and minerals. Antioxidant compounds such as total phenols (15.1 and 12.6mgGAE/100g), tannins (35.6 and 16.1mgCE/100g) and flavonoids (709 and 81.6mgCEQ/100g) were higher in ethanolic extracts over aqueous extracts, respectively. Inhibition of DPPH was high in ethanol extracts (48.9%) compared to aqueous extracts (19.7%), whereas aqueous extracts showed a higher FRAP value compared to ethanol extracts (0.98 and 0.61mmolFe(II)/100g, respectively). Results on functional properties revealed acceptable water and oil absorption capacities (5.51 and 1.98g/g, respectively), emulsion capacity and stability (15.3% and 6.90%, respectively), and foaming capacity (5.78%). FTIR spectral analysis showed seed flour to encompass major functional groups such as: amines, amides, amino acids, polysaccharides, carboxylic acids, esters and lipids. As belinjau seed flour possesses a rich nutraceutical value, it has high potential to be used as a basic raw material to develop new low cost nutritious functional foods.
  8. Bhat R, Karim AA
    J Food Sci Technol, 2014 Jul;51(7):1326-33.
    PMID: 24966426 DOI: 10.1007/s13197-012-0652-9
    Developing novel fish gelatin films with better mechanical properties than mammalian gelatin is a challenging but promising endeavor. Studies were undertaken to produce fish gelatin films by combining treatments with different sugars (ribose and lactose) followed 'by' 'and' ultraviolet (UV) radiation, as possible cross-linking agents. Increase in tensile strength and percent elongation at break was recorded, which was more significant in films without sugars that were exposed to UV radiation. Films with added ribose showed decreased solubility after UV treatment and exhibited higher swelling percentage than films with added lactose, which readily dissolved in water. FTIR spectra of all the films showed identical patterns, which indicated no major changes to have occurred in the functional groups as a result of interaction between gelatin, sugars and UV irradiation. The results of this study could be explored for commercial use, depending on industrial needs for either production of edible films or for food packaging purposes.
  9. Tan TC, Cheng LH, Bhat R, Rusul G, Easa AM
    Food Chem, 2014 Jan 1;142:121-8.
    PMID: 24001821 DOI: 10.1016/j.foodchem.2013.07.040
    Composition, physicochemical properties and enzyme inactivation kinetics of coconut water were compared between immature (IMC), mature (MC) and overly-mature coconuts (OMC). Among the samples studied, pH, turbidity and mineral contents for OMC water was the highest, whereas water volume, titratable acidity, total soluble solids and total phenolics content for OMC water were the lowest. Maturity was found to affect sugar contents. Sucrose content was found to increase with maturity, and the reverse trend was observed for fructose and glucose. Enzyme activity assessment showed that polyphenol oxidase (PPO) in all samples was more heat resistant than peroxidase (POD). Compared to IMC and MC, PPO and POD from OMC water showed the lowest thermal resistance, with D83.3°C=243.9s (z=27.9°C), and D83.3°C=129.9s (z=19.5°C), respectively.
  10. Rajan, Nithiya Shanmuga, Bhat,Rajeev, Karim, A.A.
    MyJurnal
    Unripe and ripe kundang fruits (Bouea macrophylla Griffith) is either consumed fresh or is cooked in Malaysia. In this study composition of unripe and ripe fruits (proximate, amino acids profile, minerals and heavy metal contents) were evaluated. Results obtained showed unripe kundang fruit to possess higher moisture, ash, crude lipid, crude fiber and crude protein contents than the ripe fruits. With regard to amino acid contents, unripe fruits had higher content of essential amino acids. The unripe and ripe fruits were found to be rich in essential minerals with potassium (K) to be in abundance. Heavy metals such as cadmium, nickel, mercury, lead and arsenic, were detected in trace amounts (< 5.0 mg/kg) in both unripe and ripe fruits. Through this investigation, it is concluded that both unripe and ripe fruits to posses’ adequate amount of nutritionally important compounds beneficial to human health and can be explored for commercial purposes.
  11. Annegowda HV, Bhat R, Tze LM, Karim AA, Mansor SM
    J Food Sci Technol, 2013 Jun;50(3):535-41.
    PMID: 24425949 DOI: 10.1007/s13197-011-0370-8
    We evaluated the phenolic content and antioxidant capacities of pod and seed extracts (in methanol, ethanol, and water) of an underutilized legume, Clitoria fairchildiana (Howard). The antioxidant capacity of the extracts was determined using the ferric reducing antioxidant potential assay, and the free radical-scavenging capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical-scavenging and ABTS assays. In addition, the total flavonoids, flavonols, and tannin contents were also determined. Overall, the methanol extracts of the pod contained high concentration of phenolics and showed high antioxidant capacities compared to seed extracts. In addition, a positive correlation was found between total phenol and tannin versus antioxidant capacity. Results of the present study indicate pods and seeds of C. fairchildiana to possess rich amount of natural antioxidants, and can be further explored for their possible use as a natural additive in food or in pharmaceutical industries.
  12. Thomas, Rachel, Wan-Nadiah, W.A., Bhat, Rajeev
    MyJurnal
    In the present study, six different rice varieties marketed in Penang, Malaysia (locally grown and imported) were evaluated for proximate composition, physiochemical properties and cooking qualities. Overall, 'Black rice' variety had the highest protein content (8.16%) with lowest fat content (0.07%). Between the various rice varieties investigated, thousand kernel weight varied between 16.97-19.43 g, length/breadth (l/b) ratio was between 2.09-3.75, while bulk density varied between 0.81-0.86 g/ml. Amylose content was highest (27.71%) in white rice (local, medium grain type) with lowest recorded for brown rice variety (3.36%). Results on minimum cooking time showed it to range between 10 to 31.67 minutes with the brown rice cooking the slowest. The water uptake ratio ranged between 2.33 to 3.95 and was low in glutinous rice (2.33), while gruel solid loss (range from 3.17 to 6.43) was lowest in Basmati rice variety (3.17%). The minimum cooking time was found to be negatively correlated with amylose content (r = -0.97). A positive correlation was recorded for both amylose content and l/b ratio in relation to elongation of cooked rice. These results highlight cooking and physiochemical properties of rice to be strongly dependent on their amylose content. Results generated in this study might be able to provide vital information's on identifying 'superior quality of rice' marketed in Penang, based on their proximate composition as well as on their physiochemical and cooking properties.
  13. Voon, H.C., Bhat, Rajeev, Karim, A.A., Rosma, A.
    MyJurnal
    Traditionally, in Chinese medicine, tree peony and apple flower buds are used to prepare herbal decoctions to cure various ailments. As both of these flowers are popular and used regularly, providing scientific evidence on their basic composition is a necessity. Hence, in the present study, we report the chemical composition of these two flower buds. Results revealed tree peony and apple flower buds to have high crude protein (15.73 and 26.30%), fibre (13.11 and 16.51%), and carbohydrate (57.84 and 40.63%) contents. Both the flowers had significant amounts of essential amino acids and unsaturated fatty acids. Essential minerals present in tree peony and apple flowers were potassium (1540.37 and 1125.60 mg/100 g), calcium (462.46 and 449.98 mg/100 g), magnesium (241.51 and 164.23 mg/100 g), sodium (12.75 and 20.06 mg/100 g), and phosphorus (420.00 and 590.00 mg/100 g), respectively. Heavy metals (cadmium, nickel, mercury, lead, and arsenic) were detected in trace amounts (< 0.50 mg/100 g) in both the flower buds. Results obtained indicate that both flowers could be exploited as an additional source of nutraceutical for the development of new functional foods.
  14. Annegowda HV, Bhat R, Min-Tze L, Karim AA, Mansor SM
    J Food Sci Technol, 2012 Aug;49(4):510-4.
    PMID: 23904662 DOI: 10.1007/s13197-011-0435-8
    The present study was conducted to examine the effects of sonication treatments (time intervals of 0, 15, 30, 45 and 60 min.) on phenolics and other antioxidant compounds in starfruits extracted in methanol and water. Overall, methanolic extracts exhibited significantly higher extractability, percentage inhibition of DPPH radicals, ferric reducing antioxidant property (FRAP) value, antioxidant capacity, flavonoids, total phenolics and tannins (p 
  15. Abdul Aziz NA, Wong LM, Bhat R, Cheng LH
    J Sci Food Agric, 2012 Feb;92(3):557-63.
    PMID: 25363645 DOI: 10.1002/jsfa.4606
    Mango is a highly perishable seasonal fruit and large quantities are wasted during the peak season as a result of poor postharvest handling procedures. Processing surplus mango fruits into flour to be used as a functional ingredient appears to be a good preservation method to ensure its extended consumption.
  16. Ewe JA, Wan Abdullah WN, Bhat R, Karim AA, Liong MT
    Ultrason Sonochem, 2012 Jan;19(1):160-73.
    PMID: 21775184 DOI: 10.1016/j.ultsonch.2011.06.013
    This study aimed at utilizing ultrasound treatment to further enhance the growth of lactobacilli and their isoflavone bioconversion activities in biotin-supplemented soymilk. Strains of lactobacilli (Lactobacillus acidophilus BT 1088, L. fermentum BT 8219, L. acidophilus FTDC 8633, L. gasseri FTDC 8131) were treated with ultrasound (30 kHz, 100 W) at different amplitudes (20%, 60% and 100%) for 60, 120 and 180 s prior to inoculation and fermentation in biotin-soymilk. The treatment affected the fatty acids chain of the cellular membrane lipid bilayer, as shown by an increased lipid peroxidation (P<0.05). This led to increased membrane fluidity and subsequently, membrane permeability (P<0.05). The permeabilized cellular membranes had facilitated nutrient internalization and subsequent growth enhancement (P<0.05). Higher amplitudes and longer durations of the treatment promoted growth of lactobacilli in soymilk, with viable counts exceeding 9 log CFU/mL. The intracellular and extracellular β-glucosidase specific activities of lactobacilli were also enhanced (P<0.05) upon ultrasound treatment, leading to increased bioconversion of isoflavones in soymilk, particularly genistin and malonyl genistin to genistein. Results from this study show that ultrasound treatment on lactobacilli cells promotes (P<0.05) the β-glucosidase activity of cells for the benefit of enhanced (P<0.05) isoflavone glucosides bioconversion to bioactive aglycones in soymilk.
  17. Bhat R, Kamaruddin NS, Min-Tze L, Karim AA
    Ultrason Sonochem, 2011 Nov;18(6):1295-300.
    PMID: 21550834 DOI: 10.1016/j.ultsonch.2011.04.002
    Freshly squeezed kasturi lime fruit juice was sonicated (for 0, 30 and 60min at 20°C, 25kHz frequency) to evaluate its impact on selected physico-chemical and antioxidant properties, such as pH, °Brix, titratable acidity, Hunter color values (L(∗), a(∗), b(∗)), ascorbic acid, DPPH radical scavenging activity, total phenolics, antioxidant capacity, flavonoids and flavonols. Additionally, the effect of sonication treatments on the microbial load (TPC, yeast and mold) were also evaluated. Sonication of juice samples for 60min showed enhancement in most of the bioactive compounds compared to samples treated for 30min and control samples (untreated). Significant reductions in the microbial load corresponding to sonication time were also recorded. Results of the present study indicate that sonication may be employed as a suitable technique for kasturi lime juice processing, where antioxidant and other bioactive compound retention or enhancement is desired, along with the achievement of safety and quality standards.
  18. Migeemanathan S, Bhat R, Min-Tze L, Wan-Abdullah WN
    Foodborne Pathog Dis, 2011 Nov;8(11):1235-40.
    PMID: 21819211 DOI: 10.1089/fpd.2011.0945
    The growth and survival of Salmonella typhimurium in goat milk samples at different shifting temperatures were evaluated. The growth of S. typhimurium at lower temperatures (5°C, 10°C, and 15°C) exhibited bacteriostatic effects in milk, whereas at ambient temperature (25°C) and at 45°C, this pathogen luxuriantly grew throughout the 12-h stationary phase. At 50°C this pathogen was found to be thermotolerant and could still thrive in the milk. Overall, shifting temperatures from 37°C to 55°C and 60°C clearly indicated S. typhimurium to have reached complete elimination. The results demonstrated that the adaptation and survival of this pathogen directly depend on temperature stress. It is expected that the results will be useful to dairy industries for implementation of good manufacturing practices with a better hazard analysis critical control point approach to predict the microbial risk assessment and also benefit the consumers.
  19. Aziah AA, Min WL, Bhat R
    Int J Food Sci Nutr, 2011 Sep;62(6):559-67.
    PMID: 21534889 DOI: 10.3109/09637486.2011.562883
    Sponge cake prepared by partial substitution of wheat flour with mango pulp and mango peel flours (MPuF and MPeF, respectively) at different concentrations (control, 5%, 10%, 20% or 30%) were investigated for the physico-chemical, nutritional and organoleptic characteristics. Results showed sponge cake incorporated with MPuF and MPeF to have high dietary fiber with low fat, calorie, hydrolysis and predicted glycemic index compared with the control. Increasing the levels of MPuF and MPeF in sponge cake had significant impact on the volume, firmness and color. Sensory evaluation showed sponge cake formulated with 10% MPuF and 10% MPeF to be the most acceptable. MPeF and MPuF have high potential as fiber-rich ingredients and can be utilized in the preparation of cake and other bakery products to improve the nutritional qualities.
  20. Bhat R, Ameran SB, Voon HC, Karim AA, Tze LM
    Food Chem, 2011 Jul 15;127(2):641-4.
    PMID: 23140712 DOI: 10.1016/j.foodchem.2011.01.042
    Starfruit juice were exposed to ultraviolet (UV-C) light for 0, 30 and 60min at room temperature (25±1°C). On exposure, the titratable acidity significantly decreased, while the decrease in °Brix and pH were not significant. With regard to colorimetric parameters, L(∗) value increased significantly with a subsequent decrease in a(∗) and b(∗) values corresponding to UV treatment time. Except for the ascorbic acid, other antioxidants measured (% DPPH inhibition, total phenols, flavonols, flavonoids and antioxidant capacity) showed enhancement on expsoure to UV (significant at 60min). Microbial studies showed reduction in APC, yeasts and mould counts by 2-log cycle on UV treatments. These results supports the application of UV as a measure of non-thermal and physical food preservation technique for starfruit juice that can be explored commercially to benefit both the producers and consumers.
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