Displaying publications 21 - 40 of 106 in total

Abstract:
Sort:
  1. Mixed phenotype acute leukaemia with t(9,22), BCR-ABL1: A case report
    Yong WL, Yusof N, Ithnin A, Shuib S, Tumian R, Yousuf R, et al.
    Malays J Pathol, 2020 Dec;42(3):469-476.
    PMID: 33361731
    INTRODUCTION: Mixed phenotype acute leukaemia (MPAL) is a rare entity of acute leukaemia.

    CASE REPORT: Here we report a case of a 39-year-old lady, with an incidental finding of hyperleukocytosis (white blood cells count: 139.2 x 109/L). Her peripheral blood film revealed 36% of blasts and a bone marrow aspiration showed 53% of blasts. Immunophenotyping showed a population of blasts exhibiting positivity of two lineages, myeloid lineage and B-lymphoid lineage with strong positivity of CD34 and terminal deoxynucleotidyl transferase (Tdt). A conventional karyotyping revealed the presence of Philadelphia chromosome. She was diagnosed with MPAL with t(9,22), BCR ABL1, which carried a poor prognosis. She was treated with acute lymphoblastic leukaemia (ALL) chemotherapy protocol coupled with a tyrosine kinase inhibitor and was planned for an allogeneic stem cells transplant.

    CONCLUSION: This MPAL case was diagnosed incidentally in an asymptomatic patient during medical check-up. We highlight this rare case report to raise the awareness about this rare disease. Understanding the pathogenesis of the disease with the underlying genes responsible for triggering the disease, uniform protocols for diagnosis and targeted treatment will help for proper management of these patients.

  2. Analyzing the mediating effect of economic performance on the relationship between green construction practices and health and safety performance in Nigeria
    Onubi HO, Yusof N, Hassan AS, Bahdad AAS
    Environ Sci Pollut Res Int, 2021 Jul;28(27):36598-36610.
    PMID: 33709310 DOI: 10.1007/s11356-021-13334-6
    The adoption of green construction practices (GCP) has been on the increase in recent years as a means of reducing the negative effects of construction on the natural environment. However, GCP have been discovered to expose the construction workers to numerous health and safety (HS) risks, resulting from a decline in safety investment due to the economic burden associated with its adoption. This study explores the means through which GCP influence the HS performance of construction projects through economic performance. To obtain the views of contractors, a survey questionnaire was developed, and data was collected from project managers and site managers of "class A" contractors, with a response rate of 81.55%. The partial least squares structural equation modeling (PLS-SEM) technique was adopted to analyze the data. The results show that the effect of GCP on HS performance is fully mediated by economic performance. The study concludes that for projects that adopt GCP to have high levels of HS performance, they are required to have an optimal economic performance. Efforts should be intensified by the government in providing subsidies, tax waivers, and other incentives for adopters of GCP to ensure the economic performance of their projects since it guarantees high HS performance.
  3. Fulltext Online News Coverage of the Sugar-Sweetened Beverages Tax in Malaysia: Content Analysis
    Mohd Hanim MF, Md Sabri BA, Yusof N
    JMIR Public Health Surveill, 2021 08 18;7(8):e24523.
    PMID: 34406125 DOI: 10.2196/24523
    BACKGROUND: In Malaysia, the Sugar-Sweetened Beverages (SSBs) tax was announced during the parliament's 2019 Budget Speech. The tax was slated to be enforced by April 2019 but was later postponed to July 2019. The announcement has since generated significant media coverage and public feedback.

    OBJECTIVE: This study presents a qualitative and quantitative cross-sectional study using netnography to examine how Malaysian online news articles responded to the SSBs tax after the announcement and postimplementation.

    METHODS: Online news articles published on popular online news platforms from November 2018 to August 2019 were downloaded using NCapture and imported into NVivo for analysis using the inductive approach and thematic content analysis following the initial SSBs implementation announcement.

    RESULTS: A total of 62 news articles were analyzed. Most of the articles positively portrayed the SSBs tax (46.8%) and highlighted its health impacts (76%). There were 7 key framing arguments identified in the articles. The positive arguments revolved around incentivizing manufacturers to introduce healthier products voluntarily, positive health consequences, the tax's impact on government revenue, and the use of the generated revenue toward beneficial social programs. The opposing arguments included increased operating costs to the manufacturer, the increased retail price of drinks, and how the SSBs tax is not a robust solution to obesity. The top priority sector considered in introducing the tax was the health perspective, followed by economic purposes and creating policies such as regulating the food and drinks industry.

    CONCLUSIONS: The majority of online news articles positively reported the implementation of the SSBs tax in Malaysia. This suggests media played a role in garnering support for the health policy. As such, relevant bodies can use negative findings to anticipate and reframe counteracting arguments opposing the SSBs tax.

  4. Amphetamine-type stimulants (ATS) drug classification using shallow one-dimensional convolutional neural network
    Mohd Yusof N, Muda AK, Pratama SF, Carbo-Dorca R
    Mol Divers, 2021 Aug 02.
    PMID: 34338915 DOI: 10.1007/s11030-021-10289-1
    Amphetamine-type stimulants (ATS) drug analysis and identification are challenging and critical nowadays with the emergence production of new synthetic ATS drugs with sophisticated design compounds. In the present study, we proposed a one-dimensional convolutional neural network (1DCNN) model to perform ATS drug classification as an alternative method. We investigate as well as explore the classification behavior of 1DCNN with the utilization of the existing novel 3D molecular descriptors as ATS drugs representation to become the model input. The proposed 1DCNN model is composed of one convolutional layer to reduce the model complexity. Besides, pooling operation that is a standard part of traditional CNN is not applied in this architecture to have more features in the classification phase. The dropout regularization technique is employed to improve model generalization. Experiments were conducted to find the optimal values for three dominant hyper-parameters of the 1DCNN model which are the filter size, transfer function, and batch size. Our findings found that kernel size 11, exponential linear unit (ELU) transfer function and batch size 32 are optimal for the 1DCNN model. A comparison with several machine learning classifiers has shown that our proposed 1DCNN has achieved comparable performance with the Random Forest classifier and competitive performance with the others.
  5. Effect of energy management and waste management on schedule performance: role of technological complexity and project size
    Onubi HO, Yusof N, Hassan AS, Bahdad AAS
    Environ Sci Pollut Res Int, 2022 Apr;29(19):29075-29090.
    PMID: 34993828 DOI: 10.1007/s11356-021-18376-4
    Schedule overrun is one of the greatest hindrances to construction project performance, thereby making schedule management an integral part of construction project management. The aim of this study is to examine how the effect of sustainable energy management (SEM) and sustainable waste management (SWM) on schedule performance (SP) is mediated by technological complexity (TC) and moderated by project size (PS). Data were obtained by means of a questionnaire survey of 168 completed construction projects in Nigeria. The partial least squares structural equation modelling (PLS-SEM) technique was adopted in analysing the collected data. The results show that TC partially mediates the relationship between SWM and SP, while TC fully mediates the relationship between SEM and SP. Also, the findings of the study indicate that the negative effect of SWM on SP will be greater with large PS. This study contributes to previous studies in the area of schedule management, by providing empirical proof to explain the means through which SWM and SEM could lead to SP through TC, and how this would vary depending on PS. The study proffers ways for contractors to improve the SP of their projects considering PS and TC.
  6. Enhanced photodegradation of o-cresol in aqueous Mn(1%)-doped ZnO suspensions
    Abdollahi Y, Abdullah AH, Gaya UI, Zainal Z, Yusof NA
    Environ Technol, 2012 Jun;33(10-12):1183-9.
    PMID: 22856288
    The effective removal of o-cresol is currently both an environmental and economic challenge. ZnO is not only an efficient photocatalyst but is also cost effective, as its photoabsorption can extend from the ultraviolet (UV) to the visible range thereby allowing the use of inexpensive visible light sources, such as sunlight. The principal objective of the present work is to investigate the visible light-driven removal of o-cresol from aqueous solution in the presence of 1.0 wt% Mn-doped ZnO. To measure the efficiency ofphotodegradation, the variables studied included the amount ofphotocatalyst, concentration of o-cresol, pH and irradiation time. The concentration ofo-cresol and residual organic carbon was monitored using a UV-visible spectrophotometer, ultra high-pressure liquid chromatography and a total organic carbon analyser. The optimum conditions under which the photodegradation of o-cresol was most favourable corresponded to 1.5 g/l ZnO, 35 ppm o-cresol and pH 9. The ZnO-1 wt% Mn photoprocess has demonstrated reusability for more than three times, which warrants its scale-up from laboratory- to in industrial-scale application.
  7. Longitudinal study of dental caries increment in Malaysian school children: a 5-year cohort study
    Masood M, Yusof N, Hassan MI, Jaafar N
    Asia Pac J Public Health, 2014 May;26(3):260-7.
    PMID: 22218936 DOI: 10.1177/1010539511420704
    The aim of this 5-year longitudinal cohort study was to assess the prevalence, severity, and trends in caries increment and impact of the School Dental Incremental Care Programme (SDICP). Data were gathered from school dental records as part of the SDICP. A sample of 1830 children were included and checked for caries experience annually using World Health Organization criteria. In total, 95.4% of the children were caries free in 2004, and caries experience declined to 70.5% in 2009 with an average of 4.9% annually. At baseline, the mean DMFT (confidence interval [CI]) was 0.06 (0.05-0.08) and increased to 0.58 (0.53-0.63) in 2009. Children with active caries were 4.4% in 2004, and figures rose to 9.6% in 2009. The FT component increased most rapidly during these 5 years from 0.2% to 25.1%. Overall caries prevalence and increment was low in this study. Proportions of FT component were higher as compared with DT component with low rate of extractions during the latter years of the study.
  8. Fulltext Hemolytic disease of the fetus and newborn caused by anti-D and anti-S alloantibodies: a case report
    Yousuf R, Abdul Aziz S, Yusof N, Leong CF
    J Med Case Rep, 2012;6:71.
    PMID: 22348809 DOI: 10.1186/1752-1947-6-71
    Hemolytic disease of the fetus and newborn is most commonly caused by anti-D alloantibody. It is usually seen in Rhesus D (RhD)-negative mothers that have been previously sensitized. We report here a case of hemolytic disease of the fetus and newborn in a newborn baby caused by anti-D and anti-S alloantibodies, born to a mother who was RhD negative, but with no previous serological evidence of RhD alloimmunization.
  9. Fulltext A rare case of anti-jk3 antibody detected on pre-transfusion investigation
    Yousuf R, Abdul Aziz S, Yusof N, Leong CF
    Indian J Hematol Blood Transfus, 2014 Sep;30(3):208-10.
    PMID: 25114411 DOI: 10.1007/s12288-012-0211-6
    We report a 47-year-old Malay lady, para 4 + 1, with known medical history of hypertension whom presented at Emergency Department with severe anaemia, most likely secondary to menorrhagia caused by uterine fibroids. Her haemoglobin was 5.5 g/dl and she was transfused with three units of packed cell without any adverse reaction, her haemoglobin level increased to 9.8 g/dl. She was then planned for total abdominal hysterectomy and bilateral salpingo-oophorectomy later. Four months later when she came for the elective surgery, her pre transfusion investigations showed blood group as B Rh D positive, with a probable R1R1 phenotype. Her antibody screening was positive in all the three panel cells. Further testings showed a negative Direct Coomb's test and negative autocontrol, antibody identification showed pan-agglutination reaction on all 11 panel cells with enzyme enhancement. Patient's red cell phenotype was Jk(a-b-). Anti-Jk3 was suspected and further confirmed in the reference laboratory by phenotyping as well as negative urea lysis test. This case report highlights an extremely rare but clinically significant anti-JK3 antibody detected during pretransfusion testing. This phenotype is rare in the white population, more commonly seen in various polynesians. Increased awareness among the blood bank personnel regarding the variability of the blood group phenotype and the capricious nature of the Kidd antibodies may contribute to the better management of these patients.
  10. Effects of different doses of gamma irradiation on oxygen and water vapour transmission rate of preserved human amniotic membrane
    Zahari NK, Sheikh Ab Hamid S, Yusof N
    Cell Tissue Bank, 2015 Mar;16(1):55-63.
    PMID: 24647964 DOI: 10.1007/s10561-014-9438-9
    Preserved human amniotic membrane either air dried or glycerol preserved has been used effectively to treat superficial and partial thickness wounds without leaving any obvious hypertrophic scar. The preserved amnion, sterilised by ionising radiation, is known as an effective barrier for heat, fluid and protein loss while adheres nicely on wound. Air drying slightly reduced the oxygen transmission rate (OTR) of the amnion and the value significantly dropped after 15 kGy (p < 0.05). Glycerol preservation significantly reduced (p < 0.05) the OTR indicating less oxygen transmitted through the well structured cells of the amnion. Increase in the OTR with the increasing radiation doses up to 35 kGy possibly due to direct effects of radiation that resulted in large intercellular gaps. Both preservation methods significantly increased (p < 0.05) the water vapour transmission rate (WVTR). However, the low WVTR in the air dried amnion at 15 and 25 kGy was postulated due to cross-linking of collagen. Changes in the biophysical properties can be linked to direct and indirect effects of radiation on collagen bundles. The radiation dose of 25 kGy caused no adverse effect on biophysical properties hence it is still acceptable to sterilize both the air dried and the glycerol preserved amnions.
  11. Fulltext Assessment of dental caries predictors in 6-year-old school children - results from 5-year retrospective cohort study
    Masood M, Yusof N, Hassan MI, Jaafar N
    BMC Public Health, 2012;12:989.
    PMID: 23158416 DOI: 10.1186/1471-2458-12-989
    This was a retrospective cohort study undertaken to assess the rate and pattern of dental caries development in 6-year-old school children followed-up for a period of 5 years, and to identify baseline risk factors that were associated with 5 years caries experience in Malaysian children.
  12. Scanning electron microscopic assessment on surface morphology of preserved human amniotic membrane after gamma sterilisation
    Ab Hamid SS, Zahari NK, Yusof N, Hassan A
    Cell Tissue Bank, 2014 Mar;15(1):15-24.
    PMID: 23187886 DOI: 10.1007/s10561-012-9353-x
    Human amniotic membrane that has been processed and sterilised by gamma irradiation is widely used as a biological dressing in surgical applications. The morphological structure of human amniotic membrane was studied under scanning electron microscopy (SEM) to assess effects of gamma radiation on human amniotic membrane following different preservation methods. The amniotic membrane was preserved by either air drying or submerged in glycerol before gamma irradiated at 15, 25 and 35 kGy. Fresh human amniotic membrane, neither preserved nor irradiated was used as the control. The surface morphology of glycerol preserved amnion was found comparable to the fresh amniotic membrane. The cells of the glycerol preserved was beautifully arranged, homogonous in size and tended to round up. The cell structure in the air dried preserved amnion seemed to be flattened and dehydrated. The effects of dehydration on intercellular channels and the microvilli on the cell surface were clearly seen at higher magnifications (10,000×). SEM revealed that the changes of the cell morphology of the glycerol preserved amnion were visible at 35 kGy while the air dried already changed at 25 kGy. Glycerol preservation method is recommended for human amniotic membrane as the cell morphological structure is maintained and radiation doses lower than 25 kGy for sterilization did not affect the appearance of the preserved amnion.
  13. Measuring organic carbon, nutrients and heavy metals in rivers receiving leachate from controlled and uncontrolled municipal solid waste (MSW) landfills
    Yusof N, Haraguchi A, Hassan MA, Othman MR, Wakisaka M, Shirai Y
    Waste Manag, 2009 Oct;29(10):2666-80.
    PMID: 19564103 DOI: 10.1016/j.wasman.2009.05.022
    Since landfilling is the common method of waste disposal in Malaysia, river water is greatly exposed to the risk of contamination from leachate unless proper leachate management is carried out. In this study, leachates from three different types of landfills, namely active uncontrolled, active controlled and closed controlled, were characterized, and their relationships with river water chemistry were examined monthly for a year. The influence of leachate on river water chemistry from each type of landfill depended on many factors, including the presence of a leachate control mechanism, leachate characteristics, precipitation, surface runoff and the applied treatment. The impact of leachate from an active uncontrolled landfill was the highest, as the organic content, NH(4)(+)-N, Cd and Mn levels appeared high in the river. At the same time, influences of leachate were also observed from both types of controlled landfills in the form of inorganic nitrogen (NH(4)(+)-N, NO(3)(-)-N and NO(2)(-)-N) and heavy metals (Fe, Cr, Ni and Mn). Improper treatment practice led to high levels of some contaminants in the stream near the closed controlled landfill. Meanwhile, the active controlled landfill, which was located near the coastline, was exposed to the risk of contamination resulting from the pyrite oxidation of the surrounding area.
  14. Investigation of highly unsaturated fatty acid metabolism in the Asian sea bass, Lates calcarifer
    Mohd-Yusof NY, Monroig O, Mohd-Adnan A, Wan KL, Tocher DR
    Fish Physiol Biochem, 2010 Dec;36(4):827-43.
    PMID: 20532815 DOI: 10.1007/s10695-010-9409-4
    Lates calcarifer, commonly known as the Asian sea bass or barramundi, is an interesting species that has great aquaculture potential in Asia including Malaysia and also Australia. We have investigated essential fatty acid metabolism in this species, focusing on the endogenous highly unsaturated fatty acid (HUFA) synthesis pathway using both biochemical and molecular biological approaches. Fatty acyl desaturase (Fad) and elongase (Elovl) cDNAs were cloned and functional characterization identified them as ∆6 Fad and Elovl5 elongase enzymes, respectively. The ∆6 Fad was equally active toward 18:3n-3 and 18:2n-6, and Elovl5 exhibited elongation activity for C18-20 and C20-22 elongation and a trace of C22-24 activity. The tissue profile of gene expression for ∆6 fad and elovl5 genes, showed brain to have the highest expression of both genes compared to all other tissues. The results of tissue fatty acid analysis showed that the brain contained more docosahexaenoic acid (DHA, 22:6n-3) than flesh, liver and intestine. The HUFA synthesis activity in isolated hepatocytes and enterocytes using [1-(14)C]18:3n-3 as substrate was very low with the only desaturated product detected being 18:4n-3. These findings indicate that L. calcarifer display an essential fatty acid pattern similar to other marine fish in that they appear unable to synthesize HUFA from C18 substrates. High expression of ∆6 fad and elovl5 genes in brain may indicate a role for these enzymes in maintaining high DHA levels in neural tissues through conversion of 20:5n-3.
  15. Online commentaries of the sugar-sweetened beverages tax in Malaysia: Content analysis
    Mohd Hanim MFB, Md Sabri BA, Yusof N
    Public Health Nurs, 2024;41(1):139-150.
    PMID: 37953703 DOI: 10.1111/phn.13262
    INTRODUCTION: Implementing taxes on sugary drinks, or SSBs, has been a controversial topic in many countries, including Malaysia. This study aimed to examine how Malaysian Facebook users responded to the announcement and implementation of the SSBs tax through netnography.

    METHODS: This cross-sectional study employed qualitative and quantitative methods and used an inductive approach and thematic content analysis to analyze online commentaries on news articles published on popular online news portals from November 2018 to August 2019. Data was collected by downloading the commentaries onto Microsoft Word and importing them into NVivo.

    RESULTS: Of the commentaries analyzed, 60.9% rejected the SSBs tax, and 39.1% favored it. No association was found between the online news articles and the slants of the commentaries.

    CONCLUSION: The results of this study demonstrate a clear divide in public opinion regarding the SSBs tax in Malaysia, with many online readers expressing opposition to the tax despite evidence of the harmful effects of sugar presented in the articles they are commenting on. These findings have implications for policymakers and public health advocates seeking to implement similar taxes in the future.

  16. Assessing sequence heterogeneity in Chlorellaceae DNA barcode markers for phylogenetic inference
    Wong EB, Kamaruddin N, Mokhtar M, Yusof N, Khairuddin RFR
    J Genet Eng Biotechnol, 2023 Oct 18;21(1):104.
    PMID: 37851281 DOI: 10.1186/s43141-023-00550-5
    Phylogenetic inference is an important approach that allows the recovery of the evolutionary history and the origin of the Chlorellaceae species. Despite the species' potential for biofuel feedstock production, their high phenotypic plasticity and similar morphological structures among the species have muddled the taxonomy and identification of the Chlorellaceae species. This study aimed to decipher Chlorellaceae DNA barcode marker heterogeneity by examining the sequence divergence and genomic properties of 18S rRNA, ITS (ITS1-5.8S rRNA-ITS2-28S rRNA), and rbcL from 655 orthologous sequences of 64 species across 31 genera in the Chlorellaceae family. The study assessed the distinct evolutionary properties of the DNA markers that may have caused the discordance between individual trees in the phylogenetic inference using the Robinson-Foulds distance and the Shimodaira-Hasegawa test. Our findings suggest that using the supermatrix approach improves the congruency between trees by reducing stochastic error and increasing the confidence of the inferred Chlorellaceae phylogenetic tree. This study also found that the phylogenies inferred through the supermatrix approach might not always be well supported by all markers. The study highlights that assessing sequence heterogeneity prior to the phylogenetic inference could allow the approach to accommodate sequence evolutionary properties and support species identification from the most congruent phylogeny, which can better represent the evolution of Chlorellaceae species.
  17. Fulltext Parity and housing effects on the behavioural and hypothalamic-pituitary-adrenal axis responses of pregnant ewes
    Yusof NNM, Rutherford KMD, Jarvis S, Valente L, Dwyer CM
    Animal, 2023 Nov;17(11):101006.
    PMID: 37897868 DOI: 10.1016/j.animal.2023.101006
    It is common in many countries for sheep to be housed during winter from mid-gestation until lambing to protect ewes and lambs from adverse conditions and improve late gestation nutritional management. Keeping ewes indoors, however, has its own challenges as the animals may be mixed with unfamiliar conspecifics, have limited floor and feeding space, experience changes to their diet and increased handling by humans. Therefore, the objective of this study was to investigate the effect of variation in housing management (space allowance and social stability) on the behaviour and hypothalamic-pituitary-adrenal (HPA) axis responses of pregnant ewes from mid-to-late gestation (weeks 11-18 of pregnancy). Seventy-seven ewes (41 primiparous, 36 multiparous) were divided into two groups: 'Control' and 'Restricted space and mixed' (RS-Mix), where RS-Mix ewes were allocated half the amount of space (1.27 vs 2.5 m2 for RS-Mix and Control, respectively) and feedface (concentrate feeder space) allowance (36 vs 71 cm per ewe) given to the Control group and were also subjected to two social mixing events. Aggressive behaviour at the feedface and time spent standing, lying, walking, feeding and ruminating were recorded and faecal samples were collected for assessment of faecal glucocorticoid metabolite (FGM) concentrations. Higher aggression was observed in RS-Mix ewes during the first week of observation (P = 0.044), which gradually declined to the same level as Control ewes by the end of the study (P = 0.045). RS-Mix ewes were significantly less likely to be able to freely join the feedface compared to Controls (P = 0.022). No other significant treatment effects on aggressive behaviour or FGM during gestation were found. RS-Mix ewes displayed significantly higher ruminating behaviour at week 18 of gestation compared to Control ewes (P 
  18. A review of: application of synthetic scaffold in tissue engineering heart valves
    Fallahiarezoudar E, Ahmadipourroudposht M, Idris A, Mohd Yusof N
    Mater Sci Eng C Mater Biol Appl, 2015 Mar;48:556-65.
    PMID: 25579957 DOI: 10.1016/j.msec.2014.12.016
    The four heart valves represented in the mammalian hearts are responsible for maintaining unidirectional, non-hinder blood flow. The heart valve leaflets synchronically open and close approximately 4 million times a year and more than 3 billion times during the life. Valvular heart dysfunction is a significant cause of morbidity and mortality around the world. When one of the valves malfunctions, the medical choice is may be to replace the original valves with an artificial one. Currently, the mechanical and biological artificial valves are clinically used with some drawbacks. Tissue engineering heart valve concept represents a new technique to enhance the current model. In tissue engineering method, a three-dimensional scaffold is fabricated as the template for neo-tissue development. Appropriate cells are seeded to the matrix in vitro. Various approaches have been investigated either in scaffold biomaterials and fabrication techniques or cell source and cultivation methods. The available results of ongoing experiments indicate a promising future in this area (particularly in combination of bone marrow stem cells with synthetic scaffold), which can eliminate the need for lifelong anti-coagulation medication, durability and reoperation problems.
  19. Bacillus licheniformis BT5.9 Isolated from Changar Hot Spring, Malang, Indonesia, as a Potential Producer of Thermostable α-amylase
    Ibrahim D, Zhu HL, Yusof N, Isnaeni, Hong LS
    Trop Life Sci Res, 2013 Aug;24(1):71-84.
    PMID: 24575243 MyJurnal
    A total of 34 bacterial isolates were obtained from soil samples collected from Changar Hot Spring, Malang, Indonesia. Of these, 13 isolates produced a zone of hydrolysis in starch-nutrient agar medium and generated various amylases in liquid medium. One isolate was selected as the best amylase producer and was identified as Bacillus licheniformis BT5.9. The improvement of culture conditions (initial medium pH of 5.0, cultivation temperature of 50°C, agitation speed of 100 rpm and inoculum size of 1.7 × 10(9) cells/ml) provided the highest amylase production (0.327 U/ml).
  20. Fulltext Incidence of Red Cell Alloantibody among the Transfusion Recipients of Universiti Kebangsaan Malaysia Medical Centre
    Yousuf R, Abdul Aziz S, Yusof N, Leong CF
    Indian J Hematol Blood Transfus, 2013 Jun;29(2):65-70.
    PMID: 24426338 DOI: 10.1007/s12288-012-0155-x
    Red blood cell alloimmunization is a common complication among the transfusion recipients. In Malaysia, multiple ethnicity causes genetic heterogeneity among the population which in turn can cause a wide variation of antibody. The objective of this study was to analyse the red cell alloantibody detected during the pre-transfusion testing. This was a cross-sectional study done in the blood bank of Universiti Kebangsaan Malaysia Medical Centre during the period of January-December 2010. The data was retrieved from the hospital laboratory information system. A total of 24,263 patients' blood samples were subjected for pre-transfusion testing. Antibody screening was done using an indirect antiglobulin test method. The positive samples were further identified for antibody specificity. Antibody screening tests were positive in 184 patients out of 24,263 samples with the incidence of 0.76 %. Autoantibodies and alloantibodies were detected in 39/184 (21.2 %) and 140/184 (76.1 %) of the patients respectively. In five patients (2.7 %) the antibody specificity remained undetermined. Total 161 alloantibodies were identified. The suspected Anti-Mia alloantibody was observed most frequently (49/161, 30.4 %) followed by anti-E (30/161, 18.6 %) and anti-D (22/161, 13.7 %). Anti-E and anti-c were the most common combination of multiple alloantibodies. In view of the high incidence of suspected Anti-Mia antibodies, more efforts are needed to look into the techniques for confirmation of the Anti-Mia antibodies. Besides that, we suggested that all multiply transfused patients should be phenotyped for the Rh system and to supply Rh phenotype specific blood in order to limit alloimmunization.
Related Terms
    Try searching for something
Filters
Contact Us

Please provide feedback to Administrator (afdal@afpm.org.my)

External Links