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  1. Azra MN, Wong LL, Aouissi HA, Zekker I, Amin MA, Adnan WNW, et al.
    Animals (Basel), 2024 Nov 13;14(22).
    PMID: 39535122 DOI: 10.3390/ani14223259
    The journal retracts the article titled "Crayfish Research: A Global Scientometric Analysis Using CiteSpace" [...].
  2. Younas K, Afzaal M, Saeed F, Shankar A, Kumar Bishoyi A, Khare N, et al.
    J Sci Food Agric, 2024 Oct 30.
    PMID: 39474642 DOI: 10.1002/jsfa.13953
    Each year, approximately million tons of waste is generated from eggshells disposed of in landfills. This waste is challenging to manage because of the odor and microbiological development. However, eggshells have potential applications as a solid byproduct. They can be modified and used in various industries such as metal, polymer and ceramic composites, in the production of biodiesel, heavy metal absorption from wastewater, and even as a biomaterial to substitute bone tissues. Furthermore, eggshells can be used as a cheap adsorbent for the treatment of contaminated waterways. They are also a great source of calcium and fertilizer for plants, animals and humans. Chicken eggshells can even be used as a catalyst for converting waste frying oil into biofuel. This review highlights the challenges and opportunities of eggshell waste valorization in the food industry. © 2024 Society of Chemical Industry.
  3. Xu P, Mageswaran UM, Nisaa AA, Balasubramaniam SD, Rajendran D, Ismail EHBE, et al.
    Int J Gynaecol Obstet, 2025 Apr;169(1):1-8.
    PMID: 39520180 DOI: 10.1002/ijgo.16005
    Human papillomavirus (HPV) is a sexually transmitted virus, whose persistent infection is the main reason for invasive cervical cancer (ICC), which is the fourth most common type of cancer in women, with more than 500 000 new cases every year. After infection, various alterations occur in the host, facilitating the virus's evasion of immune system clearance and promoting its proliferation. Oral probiotic consumption can influence the whole body's immunity, inflammatory reflection, neural, endocrine humoral, metabolic pathways and other organs by adjusting the components of gut microbiota (GM). Some evidence shows there is a tight connection between GM and vaginal microbiota (VM), which is referred to as the gut-vaginal axis. This review investigates the potential role of probiotics in clearing HPV via the gut-vagina axis, emphasizing the effectiveness of Lactobacillus in preventing vaginal diseases and suggesting its potential for HPV clearance. Understanding the role of probiotics in the gut-vagina axis could pave the way for new strategies to reduce and eliminate HPV and related diseases.
  4. Azizman MSA, Hanif MA, Ibrahim N, Azhari AW, Wan Ramli WK, Abdul Jalil A, et al.
    Phys Chem Chem Phys, 2024 Nov 13;26(44):27988-28001.
    PMID: 39485080 DOI: 10.1039/d4cp03248a
    Sulphur dioxide, a toxic gas pollutant, is mainly generated by the combustion of fossil fuels and the smelting of sulphur-bearing mineral ores. Removal of SO2 gas or desulphurisation can be accomplished in industries using a variety of processes; the most efficient is wet flue gas desulphurisation (FGD). However, wet FGD has challenges, such as the requirement for wastewater treatment, excessive water usage, and the necessity for chloride protective coating. Despite having a lesser adsorption capacity than wet FGD, dry FGD can efficiently remove SO2 from the effluent gas stream and avoid the issues associated with wet FGD, provided that the sorbents are modified and regenerable. An alternative dry desulphurisation strategy by using fibrous mesoporous silica (KCC-1) modified with deep eutectic solvents (DES), choline chloride-glycerol (DES1) and choline chloride-ethylene glycol (DES2) is studied in this paper. KCC-1 modified with DES1 is found to increase SO2 adsorption capacity to 4.83 mg g-1, which is 1.73 times greater than unmodified KCC-1 and twice higher than KCC-1 modified with DES2 attributed to the sorbent's high porosity. Increasing reaction temperature and SO2 concentration reduce the adsorption capacity to 1.73 mg g-1 and 2.73 mg g-1, respectively. The Avrami kinetic model and the Toth isotherm model best reflect SO2 adsorption on the modified KCC-1, indicating that SO2 molecules are adsorbed exothermically in multilayer adsorption on a heterogeneous surface through a combination of physical and chemical processes. The higher SO2 adsorption capacity of the modified KCC-1 suggests that choline chloride-glycerol can provide additional sites for SO2 adsorption in dry FGD technology.
  5. Huan NC, Kho SS, Nyanti LE, Ramarmuty HY, Rahim MAA, Ho RL, et al.
    Tuberc Respir Dis (Seoul), 2025 Jan;88(1):181-189.
    PMID: 39542010 DOI: 10.4046/trd.2024.0029
    BACKGROUND: In the absence of significant pleural effusion, conventional medical thoracoscopy (MT) is often not feasible due to the risk of lung injury. Dry MT mitigates these risks by inducing artificial pneumothorax through needle insufflation or blunt dissection. Although the Veress needle is commonly used by surgeons to create pneumoperitoneum before laparoscopic surgeries, its application in dry MT has not been widely reported in recent times.

    METHODS: We report on a series of 31 patients who underwent dry MT with artificial pneumothorax induction using Veress needle under thoracic ultrasonography (TUS) guidance. A procedure was considered technically successful if it met all the following criteria: successful pneumothorax induction, allowing smooth insertion of the semi-rigid thoracoscope; absence of immediate significant procedural-related complications; and no delayed complications such as persistent air leaks, defined as leakage lasting more than 5 days necessitating extended chest tube placement.

    RESULTS: Complete pneumothorax induction was achieved in 25 cases, resulting in an 80.6% technical success rate; however, biopsies were successfully performed in all cases. The most frequent histopathological diagnoses were malignancy (n=9, 29.0%), followed by inflammatory pleuritis (n=8, 25.8%) and tuberculosis (n=8, 25.8%). No procedural complications were reported.

    CONCLUSION: These results indicate that TUS-guided dry MT utilizing a Veress needle is technically feasible and secure when performed by experienced MT practitioners in TUS.

  6. Badaruddin IA, Othman H, Wan Shuaib WMA, Che Yusof MZ, Othman S, Anuar Ali MN, et al.
    Asia Pac J Public Health, 2024 Nov;36(8):806-809.
    PMID: 39318114 DOI: 10.1177/10105395241282621
  7. Ahmad Najib M, Winter A, Mustaffa KMF, Ong EBB, Selvam K, Khalid MF, et al.
    Sci Rep, 2024 Nov 18;14(1):28416.
    PMID: 39557915 DOI: 10.1038/s41598-024-78685-9
    Aptamers have emerged as prominent ligands in clinical diagnostics because they provide various advantages over antibodies, such as quicker generation time, reduced manufacturing costs, minimal batch-to-batch variability, greater modifiability, and improved thermal stability. In the present study, we isolated and characterized DNA aptamers that can specifically bind to the hemolysin E (HlyE) antigen of Salmonella Typhi for future development of typhoid diagnostic tests. The DNA aptamers against Salmonella Typhi HlyE were isolated using systematic evolution of ligands by exponential enrichment (SELEX), and their binding affinity and specificity were assessed utilizing enzyme-linked oligonucleotide assay (ELONA). A total of 11 distinct aptamers were identified, and the binding affinities and species selectivities of the three most probable aptamers were determined. Kd values were obtained in the nanomolar range, with the highest affinity of 83.6 nM determined for AptHlyE97. In addition, AptHlyE11, AptHlyE45 and AptHlyE97 clearly distinguished S. Typhi HlyE from other tested bacteria, such as Salmonella Paratyphi A, Salmonella Paratyphi B, Shigella flexneri, Klebsiella pneumonia and Escherichia coli, therefore displaying desirable specificity. These novel aptamers could be used as diagnostic ligands for the future development of inexpensive and effective point-of-care tests for typhoid surveillance, especially in developing countries of the tropics and subtropics.
  8. Fareed I, Chowdhury ZZ, Bin Johan MR, Salim Newaz KM, Mohd Zubir MN, Nawaz R, et al.
    RSC Adv, 2026 Oct 23.
    PMID: 42921297 DOI: 10.1039/d6ra08056a
    Aluminium oxide (Al2O3) nanocomposites are increasingly emerging in modern engineering. However, there is still a significant gap in understanding how nanoarchitectonic design can be systematically combined with performance measures across different fields. The number of studies has examined individual functionalities; there is still a lack of a clear and integrated framework that connects synthesis strategies with the optimization of material properties for applications in heat transfer, environmental remediation and biomedical systems. This review brings together recent advances by examining how processing methods influence structural development and in turn, affect interfacial interactions and material performance. We examine how different fabrication methods, including sol-gel, hydrothermal synthesis, and emerging cold sintering techniques, influence key material characteristics such as phase composition (α- and γ-Al2O3), dispersion stability and thermal resistance at interfaces. The analysis shows that the shift from simply improving mechanical strength to achieving multifunctional performance depends largely on how the Al2O3 phase is carefully distributed and integrated within the host matrix. The findings also highlight the important role of interface engineering in improving thermal conductivity in nanofluids, accelerating adsorption during wastewater treatment and enhancing controlled bioactivity in drug delivery systems. We also examine the key challenges in moving these materials toward practical applications, particularly nanoparticle agglomeration and potential toxicity. The review further emphasizes the need for cradle-to-gate life-cycle assessments (LCA) to determine whether proposed synthesis methods can genuinely be considered environmentally sustainable. By taking an integrated approach that connects processing, structure, properties and performance, this review highlights data-driven nanoarchitectonic and scalable in situ synthesis as two key priorities for future research. Overall, Al2O3 nanocomposites offer strong potential for developing more sustainable technologies. However, their successful application will depend on better control of interfacial properties, along with careful consideration of environmental safety and the development of standardized methods that can be reliably applied at an industrial scale.
  9. Shadin M, Barua MB, Ali T, Farahim F, Ripon M, Linkon MH, et al.
    Drug Dev Res, 2026 Nov;87(7):e70394.
    PMID: 42853217 DOI: 10.1002/ddr.70394
    Cholinergic dysfunction is a key feature of cognitive impairment, and currently available acetylcholinesterase (AChE) inhibitors provide limited symptomatic benefit and are often associated with adverse effects. This study evaluated the cognitive-protective effects of bavachin (BVC), a prenylated flavonoid from Psoralea corylifolia, and its interaction with Donepezil (DNP) in a scopolamine (SCP)-induced amnesia model. Male Swiss albino mice were pretreated with BVC (10-40 mg/kg), DNP (2.5 mg/kg), or their combination for 7 days prior to SCP challenge, followed by assessment using multiple behavioral paradigms, including novel object recognition, elevated plus maze, passive avoidance, Y-maze, and Morris water maze. BVC attenuated SCP-induced memory deficits in a dose-dependent manner, with the 40 mg/kg dose producing performance statistically comparable to, though not formally demonstrated to be equivalent to, the normal control across tests. Bliss independence analysis of behavioral endpoints indicated predominantly additive interactions, with occasional greater-than-additive trends. Biochemical evaluation demonstrated that BVC inhibits AChE in vitro (IC50 = 27.7 µM) and attenuates SCP-induced elevations of AChE activity ex vivo in the frontal cortex and hippocampus. Combination treatment showed a greater-than-additive interaction in the frontal cortex (ΔBliss = 0.054) and an approximately additive effect in the hippocampus (ΔBliss = 0.013). Molecular docking and molecular dynamics simulations further suggested stable binding of BVC within the active sites of AChE and butyrylcholinesterase (BChE). Collectively, these findings indicate that BVC attenuates cognitive deficits, at least in part, through cholinesterase inhibition, and exhibits additive effects with DNP, supporting its potential as an adjunctive candidate for cognitive disorders.
  10. Wai YZ, Yeoh BN, Chiang WS, Sharum MHB, Kadir NA, Jalil NHBM, et al.
    Open Vet J, 2025 Jun;15(6):2889-2894.
    PMID: 40989619 DOI: 10.5455/OVJ.2025.v15.i6.58
    BACKGROUND: Cataracts are a common ocular condition that affects captive sun bears, yet there is a lack of detailed case reports and ocular studies of this species.

    AIM: The examinations aim to calculate the intraocular lens (IOL) power required to achieve emmetropia following cataract surgery.

    METHODS: Five Bornean sun bears diagnosed with cataracts at the Bornean Sun Bear Conservation Centre in Malaysia underwent ocular examination under general anesthesia. Keratometry and biometry data, including anterior chamber depth, crystalline lens thickness, and axial globe length, were collected using an automated keratometer and B-mode ultrasound. The Retzlaff, Binkhorst, Colenbrander, and Fyodorov formulas were used to estimate the required IOL power.

    RESULTS: The mean corneal keratometric power was 56.94 D, the mean axial globe length was 12.93 mm, and the mean calculated postoperative anterior chamber depth was 4.77 mm. The mean calculated IOL power was +92.96 D. Conclusion: Aphakic cataract surgery were decided following these findings showing no available commercial IOL implants for this species. These findings provide valuable baseline data for optimizing cataract surgery in Bornean sun bears.

  11. Abdullah S, Azhar MS, Mokhtar SA, Hua LC, Fan ESZ, Maktar JF, et al.
    Surg Radiol Anat, 2025 Feb 28;47(1):85.
    PMID: 40021499 DOI: 10.1007/s00276-025-03596-x
    INTRODUCTION: Ultrasound is a primary imaging modality for diagnosing and managing nerve-related injuries, particularly for identifying peripheral nerve locations. However, its accuracy and reliability in surgical applications remain insufficiently explored, potentially limiting its utility in nerve surgery.

    PURPOSE: This study aims to assess the precision of ultrasound in identifying upper limb peripheral nerves by comparing ultrasonographic findings with direct human cadaveric dissection.

    METHOD: Fourteen fresh-frozen upper limb specimens were examined in February 2023. Ultrasound assessments were performed by two experienced musculoskeletal radiologists using three linear probes (15 MHz, 24 MHz, and a 22 MHz hockey stick probe). Seven peripheral nerves were identified and marked using dye injections. Subsequent dissections were conducted under loupe magnification by hand surgeons.

    RESULT: The recurrent motor branch of the median nerve exhibited the lowest discrepancy (mean 1.014 ± 1.459 mm) and the highest accuracy (64.29% of specimens). The greatest discrepancy was observed in the branch of the musculocutaneous nerve to the brachialis (mean 5.114 ± 3.758 mm). The interrater correlation coefficient (ICC) varied across nerve sites, ranging from - 6.298 to 0.795, with the highest ICC observed in the superficial branch of the ulnar nerve (0.795, 0.066).

    CONCLUSION: These findings suggest that ultrasound is a valid and effective tool for identifying peripheral nerve branches in the upper limb, particularly for superficial nerves. Improved accuracy in ultrasound-guided nerve identification may enhance surgical precision and reduce complications in nerve-related procedures.

  12. Alfawaz Altamimi AS, Arockia Babu M, Afzal M, Bishoyi AK, Roopashree R, Saini S, et al.
    Med Oncol, 2025 Mar 18;42(4):114.
    PMID: 40100465 DOI: 10.1007/s12032-025-02647-y
    Natural killer cell-derived exosomes (NK-Exos) hold great promise as immune modulators and immunotherapeutics against cancer due to their intrinsically latent anti-tumor effects. They use these nanosized vesicles to deliver cytotoxic molecules, such as perforin, granzymes, and miRNAs, directly to cancer cells to kill them, avoiding immune suppression. NK-Exos has particular efficacy for treating aggressive breast cancer by modulating the TME to activate the immune response and suppress immunosuppressive factors. Bioengineering advances have extended the therapeutic potential of NK-Exos, which permits precise tumor cell targeting and efficient delivery of therapeutic payloads, including small RNAs and chemotherapeutic agents. In engineered NK-Exos, sensitization of cancer cells to apoptosis, reduction of tumor growth, and resistance to drugs have been demonstrated to be highly effective. When combined, NK-Exos synergizes with radiotherapy, chemotherapy, or checkpoint inhibitors, enhancing therapeutic efficacy, and minimizing systemic toxicity. This review emphasizes the critical role of NK-Exos in breast cancer treatment, their integration into combination therapies, and the need for further research to overcome existing limitations and fully realize their clinical potential.
  13. Redhani MY, Samad R, Pasiga BD, Erwansyah E, Anwar AI, Akbar FH, et al.
    PMID: 42969419 DOI: 10.1177/10105395261494865
    Oral diseases remain a public health burden, while the integration of dental care into Universal Health Coverage (UHC) varies across countries. Evidence on dental health insurance systems in the World Health Organization (WHO) Western Pacific Region (WPR) remains heterogeneous. This scoping review mapped and synthesised evidence on dental health insurance systems and related UHC dimensions across 6 WPR countries. The review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analysis extension for Scoping Reviews (PRISMA-ScR) guidelines, using searches of electronic databases, WHO and government websites, and reference lists for evidence published from 2000 to 2025. Of 1245 database records identified, 29 sources met the inclusion criteria. Substantial variation was observed in insurance architecture, dental benefit scope, financing, access, workforce distribution, equity, and financial protection. Preventive services were commonly represented, whereas restorative and complex services varied considerably. Formal insurance enrolment did not necessarily correspond to comparable levels of effective dental service access or financial protection. The 6 countries accounted for substantial proportions of the global 2023 burden of oral diseases. Dental health insurance arrangements are diverse across WPR countries. Advancing Universal Oral Health Coverage (UOHC) requires consideration of population coverage alongside service coverage, accessibility, equity, and financial protection, while oral disease burden should be interpreted as epidemiological context rather than as an outcome attributable to insurance arrangements.
  14. Bharadwaj HR, Dahiya DS, Dalal P, Fuad M, Dhali A, Sokhal BS, et al.
    Dig Dis Sci, 2026 Mar;71(3):822-837.
    PMID: 41051645 DOI: 10.1007/s10620-025-09432-x
    BACKGROUND: Gastrointestinal (GI) and liver diseases impose a substantial burden on the United Kingdom's health system, ranking among the leading causes of mortality, cancer-related deaths, and hospital admissions. Despite the universal framework of the National Health Service (NHS), profound inequalities persist across socioeconomic, geographic, and ethnic lines.

    OBJECTIVES: This review focuses on colorectal cancer (CRC) and hepatocellular carcinoma (HCC) as exemplar conditions, synthesising current evidence on disparities across the care continuum and exploring strategies to mitigate them.

    METHODS: Current national data and published evidence were reviewed to assess inequities in disease prevalence, access to care, and outcomes across socioeconomic strata, regions, and ethnic groups. The analysis also examined workforce distribution, policy frameworks, and emerging interventions aimed at addressing these disparities.

    RESULTS: Disease prevalence and outcomes are markedly worse in socioeconomically deprived regions; liver disease mortality is more than twice as high in the poorest deciles, and CRC survival is significantly lower among disadvantaged populations. Access to care remains uneven-deprived groups exhibit lower CRC screening uptake, delayed diagnoses, and higher emergency presentation rates. Routine HCC surveillance in at-risk patients is inconsistently delivered. Workforce shortages and maldistribution exacerbate these inequities, with under-resourced regions facing longer waiting times and limited specialist access. Systemic challenges, including social determinants, data deficits, and policy underprioritisation, further hinder progress.

    EMERGING DEVELOPMENTS: Promising advances include the NHS's expansion of community diagnostic centres, targeted workforce investment, national hepatitis C virus (HCV) elimination programmes, and structural interventions such as minimum unit pricing for alcohol. Technological innovations-including non-invasive diagnostics and digital tools-offer additional opportunities to bridge care gaps.

    CONCLUSIONS: Targeted actions, such as implementing primary care FibroScan services, CRC outreach initiatives with GP endorsement, and patient navigation for HCC surveillance, are critical to reducing inequalities and improving outcomes. By addressing upstream determinants and ensuring that innovations reach under-served populations, the UK can progress toward reducing GI health inequalities, improving outcomes, and achieving more uniform digestive health across all communities.

  15. Mujar EK, Matusin S, Yahya NS, Bustamam MSA, Christianus A, Shaari K, et al.
    Fish Physiol Biochem, 2025 May 19;51(3):99.
    PMID: 40388052 DOI: 10.1007/s10695-025-01510-1
    Aquaculture, which involves the cultivation of aquatic animals and plants, is recognised to be an effective and vital resource-efficient food production system for supplying affordable protein to humans. Hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) is a marine carnivorous teleost that has a high economic importance in the marine aquaculture industry particularly in countries in the Asian region. However, the vulnerability of juvenile hybrid grouper to temperature fluctuations stemming from the acceleration of climate change is regarded as one of significant threats to their market supply and a major concern for the aquaculture industry. In the present study, LCMS/MS-based mucus and sera metabolomics were used to investigate the physiological responses of juvenile hybrid grouper via their behavioural response towards temperature fluctuation stress that could be translated as indicators for either resilience or susceptibility to the stressors. Susceptible fish exhibited pronounced behavioural responses, including cessation of feeding and erratic swimming. In contrast, resilient fish displayed relatively stable behaviour, comparable to control fish, with reduced feeding as a primary deviation. A total of 36 mucus metabolites (VIP > 1) were profiled in OPLSDA in juvenile hybrid grouper subjected to temperature fluctuation stress. Among these, 20 and 16 metabolites were significantly altered (Student's t-test, p  1) were profiled, with 38 and 26 significantly altered in the susceptible (SSG) and resilient (SRG) groups, respectively. Comparative analysis between significant and resilient groups resulted in the nomination of hypoxanthine, guanosine, guanine, methionine, DL-malic acid, and glucose as potential biomarkers for susceptibility in juvenile hybrid grouper. Pathway analysis of these metabolite markers revealed that temperature stress mainly affected purine metabolism, TCA cycle, cysteine and methionine metabolism, and pyruvate metabolism. Overall, the findings of this study provide insights into the physiological regulation of juvenile hybrid grouper that are susceptible to temperature changes through their behavioural response, as well as a framework for future research.
  16. Baticulon RE, Esguerra JML, Lazuardy MA, Kho GS, Low SYY, My LQ, et al.
    Neurosurg Focus, 2025 Mar 01;58(3):E13.
    PMID: 40022759 DOI: 10.3171/2024.12.FOCUS24816
    OBJECTIVE: The number of pediatric neurosurgeons worldwide remains inadequate. Opportunities for fellowship training and continuing medical education in pediatric neurosurgery are limited, particularly for neurosurgeons in low- and middle-income countries. This study aimed to describe the setup and conduct of a recurring online meeting to discuss diagnostic and treatment dilemmas in pediatric neurosurgery, organized by a group of pediatric neurosurgeons in Southeast Asia.

    METHODS: Available meeting reports since inception in 2022 and registration data for the year 2024 were analyzed. Meeting recordings were reviewed to describe the cases presented for opinion. A focus group discussion was conducted among core team members to evaluate the virtual meetings.

    RESULTS: From January 2022 to August 2024, the Southeast Asian pediatric neurosurgery group organized 16 online meetings to discuss 50 patients. Based on the report for 12 meetings (75%), the mean number of participants for each session was 45 ± 11, with 91% ± 5% of the attendees being present for at least 15 minutes. Review of 2024 registration data showed that 142 unique participants from 15 countries attended the 5 meetings for this year. Most were neurosurgery residents (65%) and neurosurgery consultants (23%). Of 50 cases presented, the majority were tumors (52%) and craniofacial disorders (16%). Discussions centered on most probable diagnosis (34%), best treatment option (90%), and recommended surgical approach (82%).

    CONCLUSIONS: International case discussions in pediatric neurosurgery are feasible and sustainable online, with the potential to improve service delivery, strengthen the workforce, and grow professional networks. Leadership and time commitment from a core group are essential to ensure the success of these global neurosurgery collaborations.

  17. Balaraman AK, Arockia Babu M, Afzal M, Sanghvi G, M M R, Gupta S, et al.
    Regen Ther, 2025 Mar;28:558-572.
    PMID: 40034540 DOI: 10.1016/j.reth.2025.01.019
    Recently, increasing interest has been in utilizing mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially exosomes, as nanocarriers for miRNA delivery in cancer treatment. Due to such characteristics, nanocarriers are specific: biocompatible, low immunogenicity, and capable of spontaneous tumor accumulation. MSC-EVs were loaded with therapeutic miRNAs and minimized their susceptibility to degradation by protecting the miRNA from accessibility to degrading enzymes and providing targeted delivery of the miRNAs to the tumor cells to modulate oncogenic pathways. In vitro and in vivo experiments suggest that MSC-EVs loaded with miRNAs may inhibit tumor growth, prevent metastasis, and increase the effectiveness of chemotherapy and radiotherapy. However, these improvements present difficulties such as isolation, scalability, and stability of delivered miRNA during storage. Furthermore, the issues related to off-target effects, as well as immunogenicity, can be a focus. The mechanisms of miRNA loading into MSC-EVs, as well as their targeting efficiency and therapeutic potential, can be outlined in this manuscript. For the final part of the manuscript, the current advances in MSC-EV engineering and potential strategies for clinical application have been described. The findings of MSC-EVs imply that they present MSC-EVs as a second-generation tool for precise oncology.
  18. Gupta S, Afzal M, Agrawal N, Almalki WH, Rana M, Gangola S, et al.
    Biogerontology, 2025 Feb 26;26(2):65.
    PMID: 40011269 DOI: 10.1007/s10522-025-10207-0
    Aging and metabolic disorders share intricate molecular pathways, with the Forkhead box O (FOXO)- Sirtuin 1 (SIRT1) axis emerging as a pivotal regulator of cellular stress adaptation, metabolic homeostasis, and longevity. This axis integrates nutrient signaling with oxidative stress defence, modulating glucose and lipid metabolism, mitochondrial function, and autophagy to maintain cellular stability. FOXO transcription factors, regulated by SIRT1 deacetylation, enhance antioxidant defence mechanisms, activating genes such as superoxide dismutase (SOD) and catalase, thereby counteracting oxidative stress and metabolic dysregulation. Recent evidence highlights the dynamic role of reactive oxygen species (ROS) as secondary messengers in redox signaling, influencing FOXO-SIRT1 activity in metabolic adaptation. Additionally, key redox-sensitive regulators such as nuclear factor erythroid 2-related factor 2 (Nrf2) and Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) interact with this pathway, orchestrating mitochondrial biogenesis and adaptive stress responses. Pharmacological interventions, including alpha-lipoic acid (ALA), resveratrol, curcumin and NAD+ precursors, exhibit therapeutic potential by enhancing insulin sensitivity, reducing oxidative burden, and restoring metabolic balance. This review synthesizes current advancements in FOXO-SIRT1 regulation, its emerging role in redox homeostasis, and its therapeutic relevance, offering insights into future strategies for combating metabolic dysfunction and aging-related diseases.
  19. Hafilah Wan Ariffin WN, Sidek LM, Basri H, Idros N, Adrian MT, Abd Ghani NH, et al.
    PLoS One, 2025;20(2):e0311181.
    PMID: 40014607 DOI: 10.1371/journal.pone.0311181
    Climate change poses an escalating threat to the safety of high-hazard embankment dams, increases flood discharge impacting dam overtopping risk by altering the hydrological load of the original dam designed capacity. This paper's primary aims are to evaluate climate change's influence on extreme rainfall events and their impact on dam safety and to assess the overtopping risk of Batu Dam under various climate scenarios. This study focusses on assessing the overtopping risk of Batu Dam in Malaysia, utilizing regional climate model projections from the Coupled Model Intercomparison Project 5 (CMIP5) spanning 2020 to 2100. Three Representative Concentration Pathways (RCPs)-RCP4.5, RCP6.0, and RCP8.5 as the scenario and divide into 3 period of study: early century (2020-2046), mid (2047-2073) and late-century (2074-2100) evaluated with hydrological analysis to access the dam safety. Using the Linear Scaling Method (LSM), we corrected the bias projection rainfall data from three Regional Climate Models (RCMs) for the RCPs. Future Probable Maximum Precipitation (PMP) was estimated using statistical analysis techniques developed by the National Hydraulic Research Institute of Malaysia (NAHRIM). Additionally, Rainfall Intensity-Duration-Frequency (IDF) curves were updated based on climate scenarios outlined in the Hydrological Procedure 2021 and the associated Climate Change Factors. The HEC-HMS hydrological model was employed to simulate PMF and IDF for ARIs ranging from 1 to 100,000 years, providing a comprehensive analysis of risks under future climatic conditions. Across all future climate scenarios, inflow events were projected to exceed the dam design inflow, with RCP8.5 indicating the highest inflow values, particularly later in the century, highlighting probability of overtopping risks. Late-century projections show inflow for ARI 50 under RCP8.5 exceeding PMF by 20%, while mid-century RCP6.0 results indicate a 15% higher inflow for ARI 50000. Early-century RCP4.5 shows a 10% increase for ARI 100000 compared to PMF. The study advocates adaptive dam safety management and flood protection measures. This research provides crucial insights for embankment dam owners, stressing the urgent need to address Batu Dam's vulnerability to extreme flooding amidst climate change and emphasizing proactive measures to fortify critical infrastructure and protect downstream communities.
  20. Tomeh A, Yusof Khan AHK, Abu Zaid Z, Ling KH, Inche Mat LN, Basri H, et al.
    Medicine (Baltimore), 2025 Mar 21;104(12):e41947.
    PMID: 40128024 DOI: 10.1097/MD.0000000000041947
    Collaboration among health and non-health professionals is growing exponentially as we approach the personalized medicine era, where the intervention plan is tailored according to the patient's needs. This collaboration aims to develop highly efficient, patient-centered, holistic approaches, rather than singular interventions. Interdisciplinary rehabilitation is a rising theme to coordinate the efforts of various professionals, with the ultimate goal of increasing rehabilitants' satisfaction and improving their overall quality of life. A typical rehabilitation team may comprise a rehabilitation physician, rehabilitation nurse, occupational therapist, physiotherapist, speech and language therapist, clinical psychologist, social worker, prosthetist, orthotist, rehabilitation engineer, and dietician. The need for inclusion of additional professions in the rehabilitation team is dynamic and varies depending on the population and health condition. Recently, various countries have begun incorporating interdisciplinary rehabilitation models into their healthcare frameworks. For example, the U.S. Veterans Affairs Polytrauma Rehabilitation Centers have set a precedent for integrating interdisciplinary approaches into neurological rehabilitation, while European nations such as Germany and Sweden have successfully implemented stroke and pain rehabilitation programs. Although interdisciplinary rehabilitation has demonstrated effectiveness in improving patient outcomes, further research is required to explore its long-term benefits, cost-effectiveness, and adaptability in resource-limited settings. In this mini-review, we summarize the current evidence on employing interdisciplinary rehabilitation in patients with neurological disorders and highlight the implications for future research and clinical practice.
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