Displaying publications 81 - 100 of 171 in total

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  1. Fulltext Protein Composition and Biomedical Potential of the Skin Secretion of Hylarana erythraea (Schlegel, 1837) (Anura: Ranidae) from Langkawi Archipelago, Kedah, Peninsular Malaysia
    Ong D, Ismail MN, Shahrudin S
    Int J Pept Res Ther, 2021;27(3):2125-2133.
    PMID: 34177403 DOI: 10.1007/s10989-021-10240-w
    The skin secretion of amphibians is known for its high content of bioactive compounds. These bioactive compounds are essential for the advancement of biomedical industries. Four wild green paddy frogs, Hylarana erythraea, were collected from various habitat types within the Langkawi Archipelago. These frogs' skin secretions were collected, extracted, and analysed for their protein compounds together with biomedical potentials using liquid chromatography-mass spectrometry (LC-MS). The total protein concentration of H. erythraea skin secretions was determined as 0.269 mg/mL. Based on the UniProt (Anura) database, we identified 29 proteins. These proteins were categorised as antimicrobial (AMP) (38%), followed by hormone (17%), enzyme (17%), unreviewed proteins (17%), structural proteins (7%), and regulatory proteins (4%). The AMPs identified were from the family of esculentin-1, esculentin-2, brevinin-1, and frenatin-4, while the hormones belonged to the cholecystokinin group. The enzymes detected were adenylate cyclase 9, the suppressor of tumorigenicity 14 protein homolog, and the HGF activator. The structural proteins belonged to toe pad keratin 2 and Krt5.7 proteins, while the single regulatory protein is CCR4-NOT transcription complex subunit 6-like. These proteins have a wide range of biomedical importance, such as wound healings, facilitate digestions, anti-tumours, and anti-cancer effect.
  2. Quality of Life Among Malaysian Parents with Autism Spectrum Disorder Child: The Double ABCX Model Approach
    Ismail MF, Safii R, Saimon R, Rahman MM
    J Autism Dev Disord, 2022 Jan;52(1):113-123.
    PMID: 33635421 DOI: 10.1007/s10803-021-04929-6
    This paper reported the quality of life (QoL) of 260 Malaysian parents with autism spectrum disorder (ASD) and factors which influencing it. About half of these parents reported to having good QoL. The factors identified in the double ABCX model showed that perceived social support and sense of coherence (SOC) were mediated by coping strategies and had a positive direct and indirect effect on parents' QoL. These finding reiterate the importance of social support, coping skills, and strong SOC reflection skills in influencing the QoL of parents with ASD child. We therefore suggest parents to utilize the widely available social and organization supports to gain good QoL, which is also important for the wellbeing of their child.
  3. A laudable strategy to manage bacterial panicle blight disease of rice using biocontrol agents
    Ngalimat MS, Mohd Hata E, Zulperi D, Ismail SI, Ismail MR, Mohd Zainudin NAI, et al.
    J Basic Microbiol, 2023 Nov;63(11):1180-1195.
    PMID: 37348082 DOI: 10.1002/jobm.202300182
    Bacterial panicle blight (BPB) disease is a dreadful disease in rice-producing countries. Burkholderia glumae, a Gram-negative, rod-shaped, and flagellated bacterium was identified as the primary culprit for BPB disease. In 2019, the disease was reported in 18 countries, and to date, it has been spotted in 26 countries. Rice yield has been reduced by up to 75% worldwide due to this disease. Interestingly, the biocontrol strategy offers a promising alternative to manage BPB disease. This review summarizes the management status of BPB disease using biological control agents (BCA). Bacteria from the genera Bacillus, Burkholderia, Enterobacter, Pantoea, Pseudomonas, and Streptomyces have been examined as BCA under in vitro, glasshouse, and field conditions. Besides bacteria, bacteriophages have also been reported to reduce BPB pathogens under in vitro and glasshouse conditions. Here, the overview of the mechanisms of bacteria and bacteriophages in controlling BPB pathogens is addressed. The applications of BCA using various delivery methods could effectively manage BPB disease to benefit the agroecosystems and food security.
  4. The effect of salinity on chlorophyll, proline and mineral nutrients in common weeds of coastal rice fields in Malaysia
    Hakim MA, Juraimi AS, Hanafi MM, Ismail MR, Rafii MY, Aslani F, et al.
    J Environ Biol, 2014 Sep;35(5):855-64.
    PMID: 25204059
    Six weed species (Leptochola chinensis, Echinochloa crus-galli, Echinochloa colona, Jussiaea linifolia, Oryza sativa (weedy rice) and Cyperus iria) were tested for their salt tolerant traits in terms of chlorophyll, proline and mineral nutrients accumulation against different salinity levels (0, 4, 8, 16, 24, 32, and 40 dS m(-1)). Chlorophyll a, b and total chlorophyll content, proline and mineral nutrients accumulation were determined. Salt stress showed prominent effect on all the parameters investigated and there were significant variations between the all weed species. Chlorophyll content, K+, Ca(2+) and Mg(2+) ions in both shoots and roots significantly decreased; while proline and Na+ accumulation significantly increased with increasing salinity up to 40 dS m(-1). In terms of overall performance, Cyperus iria and E. crus-galliwere relatively more tolerant; E. colona and J. linifolia were tolerant; L. chinensis and O. sativa L were salt sensitive, respectively.
  5. Effect of salt stress on morpho-physiology, vegetative growth and yield of rice
    Hakim MA, Juraimi AS, Hanafi MM, Ali E, Ismail MR, Selamat A, et al.
    J Environ Biol, 2014 Mar;35(2):317-26.
    PMID: 24665756
    Selection of salt tolerant rice varieties has a huge impact on global food supply chain. Five Malaysian rice (Oryza sativa L.) varieties, MR33, MR52, MR211, MR219 and MR232 were tested in pot experiment under different salinity levels for their response in term of vegetative growth, physiological activities, development of yield components and grain yield. Rice varieties, BRRI dhan29 and IR20 were used as a salt-sensitive control and Pokkali was used as a salt-tolerant control. Three different salinity levels viz. 4, 8, and 12 dS m(-1) were used in a randomized complete block design with four replications under glass house conditions. Two Malaysia varieties, MR211 and MR232 performed better in terms of vegetative growth (plant height, leaf area plant(-1), number of tillers plant(-1), dry matter accumulation plant(-1)), photosynthetic rate, transpiration rate, yield components, grain yield and injury symptoms. While, MR33, MR52 and MR219 verities were able to withstand salinity stress over salt-sensitive control, BRRI dhan29 and IR20.
  6. Studies on seed germination and growth in weed species of rice field under salinity stress
    Hakim MA, Juraimi AS, Hanafi MM, Selamat A, Ismail MR, Karim SM
    J Environ Biol, 2011 Sep;32(5):529-36.
    PMID: 22319865
    An investigation was made to see the salt tolerance of 10 weed species of rice. Properly dried and treated seeds of weed species were placed on 9 cm diameter petridishes lined with Whatman No. 1 filter paper under 6 salinity regimes, viz. 0 (control), 4, 8, 16, 24 and 32 dS m(-1). The petri dishes were then kept in germinator at 25 +/- 1.0 degrees C and 12 hr light. The number of germinated seeds were recorded daily. The final germination percentage, germination index (GI), seedling vigour index, mean germination time and time for 50% germination were estimated. Root and shoot lengths of the weed seedlings were measured at 20 days after salt application and relative growth values were calculated. Results revealed that salinity decreased final germination percentage, seed of germination as measured by GI, and shoot and root length in all the species. Germination of most of the weed seeds was completely arrested (0) at 32 dS m(-1) salinity except in E. colona (12%) and C. iria (13.9%). The species C. iria, E. colona, J. linifolia and E. crusgalli showed better germination (above 30%) upto 24 dS m(-1) salinity level and were regarded as salt-tolerant weed species. J. linifolia, F. miliacea, L. chinensis and O. sativa L. (weedy rice) were graded as moderately tolerant and S. zeylanica, S. grosus and C. difformis were regarded as least tolerant weed species.
  7. Relative salinity tolerance of warm season turfgrass species
    Uddin KM, Juraimi AS, Ismail MR, Othman R, Rahim AA
    J Environ Biol, 2011 May;32(3):309-12.
    PMID: 22167942
    Fresh water, coupled with soil salinization in many areas has resulted in an increased need forscreening of salt tolerant turf grasses. Relative salinity tolerance of eightwarm season turfgrass species were examined in this study in sand culture. Grasses were grown in a glasshouse, irrigated with either distilled water or saline sea water adjusted to 24, 48 or 72 dSm-1. Salt tolerances of the grasses were assessed on the basis of their shoot and root growth, leaf firing and turf quality. Regression analysis indicated that Zoysiajaponica (Japanese lawn grass) (JG), Stenotaphrum secundatum (St. Augustine) (SA), Cynodon dactylon (satiri) (BS), Zoysia teneuifolia (Korean grass) (KG), Digitaria didactyla (Serangoon grass) (SG), Cynodon dactylon (Tifdwarf) (TD), Paspalum notatum (Bahia grass) (BG) and Axonopus compressus(Pearl blue) (PB) suffered a 50% shoot growth reduction at 36.0, 31.8, 30.9, 28.4, 26.4, 25.7, 20.0 and 18.6 dSm1 of salinity, respectively and a root growth reduction at44.9, 43.7, 33.4, 31.0, 29.5 27.5, 21.5 and 21.4 dSm- of salinity, respectively. Leaf firing and turf quality of the selected species, as a whole, were also found to be affected harmoniously with the change in root and shoot growth. On the basis of the experimental results the selected species were ranked for salinity tolerance as JG>SA>BS>KG>SG >TD>BG>PB.
  8. Fulltext Synthesis and application of functionalized Graphene oxide-silica with chitosan for removal of Cd (II) from aqueous solution
    Azizkhani S, Hussain SA, Abdullah N, Ismail MHS, Mohammad AW
    J Environ Health Sci Eng, 2021 Jun;19(1):491-502.
    PMID: 34150253 DOI: 10.1007/s40201-021-00622-z
    The functionalized graphene oxide by silica and chitosan helped to prepared an adsorbent with high adsorption potential for removing cadmium(II). In this study, the adsorbent was synthesized and the batch system of adsorption method was examined to find the potential of the new adsorbent with the various factors of the concentration, pH, time and temperature. The characterization of adsorbent was analyzed by FT-IR, TEM, Zeta potential and XRD analysis. Regards to the analysis it can be understood that the adsorbent was synthesized successfully. The investigational results were validated and analyzed by applying the 5 models of isotherm and 4 models of kinetic. The Langmuir, Freundlich, Temkin, Harkins-Jura and Dubinin-radushkevich models were used which the Langmuir, Freundlich and Temkin fitted well for removing cadmium(II). The Qmax value was achieved 126.58 mg/g by using the Langmuir model for removing Cd(II) respectively. The pseudo-first-order, pseudo-second-order, Elovich and Intra-particle models were used to validate the kinetic models of the process. The pseudo-second-order and Elovich models were the best fitted kinetic model in this investigation. Thermodynamic parameters of the energy of gibes, the enthalpy, and the entropy were calculated. Generally, the adsorption process was distinguished as an exothermic and spontaneous.
  9. Optimizing timber transportation planning for timber harvesting using bees algorithm in Malaysia
    Jamaluddin J, Kamarudin N, Ismail MH, Ahmad SA
    J Environ Manage, 2023 Apr 20;340:117977.
    PMID: 37086558 DOI: 10.1016/j.jenvman.2023.117977
    Suitable extraction technique and the least cost while reducing the environmental impact is the primary concern in timber transportation planning in undulate topography. Two types of extraction machines with unique characteristics to be applied in timber harvest area in Malaysia is combined for timber harvesting with the aim each machine will extract timber suitable to their ability. A Bees Algorithm (BA) was proposed to find an optimum TTP for timber extraction, forest road, and landing locations with grid cell-sized 10 m × 10 m and attributed with fixed and variable costs. The result shows the log fisher (1351 timbers) as a preferable extraction technique with total cost of RM 86,551.73 than the crawler tractor (206 timbers); the timber extraction route is 2630 m for the log fisher and 9860 m for the crawler tractor with total cost of RM 10,453.03. The model finds a suitable timber extraction technique and estimates the extraction costs. Further studies are required to compare the BA with other optimization methods for better results.
  10. Extraction and identification of biologically important proteins from the medicinal plant God's crown (Phaleria macrocarpa)
    Habib MAH, Ismail MN
    J Food Biochem, 2021 07;45(7):e13817.
    PMID: 34137461 DOI: 10.1111/jfbc.13817
    The fruit and leaf of God's crown (Phaleria macrocarpa) have been traditionally used to treat a wide variety of diseases. However, the proteins of this tropical plant are still heavily understudied. Three protein extraction methods; phenol (Phe), trichloroacetic acid (TCA)-acetone-phenol (TCA-A-Phe), and ultrasonic (Ult) were compared on the fruit and leaf of P. macrocarpa. The Phe extraction method showed the highest percentage of recovered protein after the resolubilization process for both leaf (12.24%) and fruit (30.41%) based on protein yields of the leaf (6.15 mg/g) and fruit (36.98 mg/g). Phe and TCA-A-Phe extraction methods gave well-resolved bands over a wide range of molecular weights through sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Following liquid chromatography-tandem mass spectrometry analysis, proteins identified through the Phe extraction method were 30%-35% enzymatic proteins, including oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases that possess various biological functions. PRACTICAL APPLICATIONS: Every part of God's crown plant is traditionally consumed to treat various illnesses. While plant's benefits are well known and have led to a plethora of health products, the proteome remains mostly unknown. This study compares three protein extraction methods for the leaf and fruit of P. macrocarpa and identifies their proteins thru LC-MS/MS coupled with PEAKS. These method comparisons can be a guide for works on other plants as well. In addition, the proteomics data from this study may shed light on the functional properties of these plant parts and their products.
  11. Fulltext Anthropometry of Malaysian young adults
    Karmegam, Karuppiah, Salit MS, Ismail MY, Ismail N, Tamrin SB, et al.
    J Hum Ergol (Tokyo), 2011 Dec;40(1-2):37-46.
    PMID: 25665206 DOI: 10.11183/jhe.40.37
    This paper presents the results of an anthropometric data collected from polytechnic students in Malaysia. A total of 1032 (595 males and 437 females) students participated in the study. Their ages ranged from 18 to 24 years. A total of 34 anthropometric dimensions were measured. Descriptive statistics such as mean, standard deviation, standard error of mean, coefficient of variation, minimum, maximum and percentile for each parameter were estimated. In addition, the comparison between Malaysia anthropometric data and Thailand (South) anthropometric data were also presented. The results show that there is a total of 12 and 11 (of dimensions parameters) significant differences (p < 0.05) between the male and female adults respectively.
  12. Fulltext Comparison of Optimization-Modelling Methods for Metabolites Production in Escherichia coli
    Lee MK, Mohamad MS, Choon YW, Mohd Daud K, Nasarudin NA, Ismail MA, et al.
    J Integr Bioinform, 2020 May 06;17(1).
    PMID: 32374287 DOI: 10.1515/jib-2019-0073
    The metabolic network is the reconstruction of the metabolic pathway of an organism that is used to represent the interaction between enzymes and metabolites in genome level. Meanwhile, metabolic engineering is a process that modifies the metabolic network of a cell to increase the production of metabolites. However, the metabolic networks are too complex that cause problem in identifying near-optimal knockout genes/reactions for maximizing the metabolite's production. Therefore, through constraint-based modelling, various metaheuristic algorithms have been improvised to optimize the desired phenotypes. In this paper, PSOMOMA was compared with CSMOMA and ABCMOMA for maximizing the production of succinic acid in E. coli. Furthermore, the results obtained from PSOMOMA were validated with results from the wet lab experiment.
  13. Fulltext In silico gene knockout prediction using a hybrid of Bat algorithm and minimization of metabolic adjustment
    Man MY, Mohamad MS, Choon YW, Ismail MA
    J Integr Bioinform, 2021 Aug 04;18(3).
    PMID: 34348418 DOI: 10.1515/jib-2020-0037
    Microorganisms commonly produce many high-demand industrial products like fuels, food, vitamins, and other chemicals. Microbial strains are the strains of microorganisms, which can be optimized to improve their technological properties through metabolic engineering. Metabolic engineering is the process of overcoming cellular regulation in order to achieve a desired product or to generate a new product that the host cells do not usually need to produce. The prediction of genetic manipulations such as gene knockout is part of metabolic engineering. Gene knockout can be used to optimize the microbial strains, such as to maximize the production rate of chemicals of interest. Metabolic and genetic engineering is important in producing the chemicals of interest as, without them, the product yields of many microorganisms are normally low. As a result, the aim of this paper is to propose a combination of the Bat algorithm and the minimization of metabolic adjustment (BATMOMA) to predict which genes to knock out in order to increase the succinate and lactate production rates in Escherichia coli (E. coli).
  14. Fulltext Artificial Bee Colony algorithm in estimating kinetic parameters for yeast fermentation pathway
    Ismail AM, Remli MA, Choon YW, Nasarudin NA, Ismail NN, Ismail MA, et al.
    J Integr Bioinform, 2023 Jun 01;20(2).
    PMID: 37341516 DOI: 10.1515/jib-2022-0051
    Analyzing metabolic pathways in systems biology requires accurate kinetic parameters that represent the simulated in vivo processes. Simulation of the fermentation pathway in the Saccharomyces cerevisiae kinetic model help saves much time in the optimization process. Fitting the simulated model into the experimental data is categorized under the parameter estimation problem. Parameter estimation is conducted to obtain the optimal values for parameters related to the fermentation process. This step is essential because insufficient identification of model parameters can cause erroneous conclusions. The kinetic parameters cannot be measured directly. Therefore, they must be estimated from the experimental data either in vitro or in vivo. Parameter estimation is a challenging task in the biological process due to the complexity and nonlinearity of the model. Therefore, we propose the Artificial Bee Colony algorithm (ABC) to estimate the parameters in the fermentation pathway of S. cerevisiae to obtain more accurate values. A metabolite with a total of six parameters is involved in this article. The experimental results show that ABC outperforms other estimation algorithms and gives more accurate kinetic parameter values for the simulated model. Most of the estimated kinetic parameter values obtained from the proposed algorithm are the closest to the experimental data.
  15. Oncologist-led BRCA counselling improves access to cancer genetic testing in middle-income Asian country, with no significant impact on psychosocial outcomes
    Yoon SY, Wong SW, Lim J, Ahmad S, Mariapun S, Padmanabhan H, et al.
    J Med Genet, 2022 Mar;59(3):220-229.
    PMID: 33526602 DOI: 10.1136/jmedgenet-2020-107416
    BACKGROUND: Identifying patients with BRCA mutations is clinically important to inform on the potential response to treatment and for risk management of patients and their relatives. However, traditional referral routes may not meet clinical needs, and therefore, mainstreaming cancer genetics has been shown to be effective in some high-income and high health-literacy settings. To date, no study has reported on the feasibility of mainstreaming in low-income and middle-income settings, where the service considerations and health literacy could detrimentally affect the feasibility of mainstreaming.

    METHODS: The Mainstreaming Genetic Counselling for Ovarian Cancer Patients (MaGiC) study is a prospective, two-arm observational study comparing oncologist-led and genetics-led counselling. This study included 790 multiethnic patients with ovarian cancer from 23 sites in Malaysia. We compared the impact of different method of delivery of genetic counselling on the uptake of genetic testing and assessed the feasibility, knowledge and satisfaction of patients with ovarian cancer.

    RESULTS: Oncologists were satisfied with the mainstreaming experience, with 95% indicating a desire to incorporate testing into their clinical practice. The uptake of genetic testing was similar in the mainstreaming and genetics arm (80% and 79%, respectively). Patient satisfaction was high, whereas decision conflict and psychological impact were low in both arms of the study. Notably, decisional conflict, although lower than threshold, was higher for the mainstreaming group compared with the genetics arm. Overall, 13.5% of patients had a pathogenic variant in BRCA1 or BRCA2, and there was no difference between psychosocial measures for carriers in both arms.

    CONCLUSION: The MaGiC study demonstrates that mainstreaming cancer genetics is feasible in low-resource and middle-resource Asian setting and increased coverage for genetic testing.

  16. Fulltext Recovery of Palm Oil and Valuable Material from Oil Palm Empty Fruit Bunch by Sub-critical Water
    Ahmad Kurnin NA, Shah Ismail MH, Yoshida H, Izhar S
    J Oleo Sci, 2016;65(4):283-9.
    PMID: 27041513 DOI: 10.5650/jos.ess15209
    Oil palm empty fruit bunch (EFB) is one of the solid wastes produced in huge volume by palm oil mill. Whilst it still contains valuable oil, approximately 22.6 million tons is generated annually and treated as solid waste. In this work, sub-critical water (sub-cw) was used to extract oil, sugar and tar from spikelet of EFB. The spikelet was treated with sub-cw between 180-280°C and a reaction time of 2 and 5 minutes. The highest yield of oil was 0.075 g-oil/g-dry EFB, obtained at 240°C and reaction time of 5 minutes. Astonishingly, oil that was extracted through this method was 84.5% of that obtained through Soxhlet method using hexane. Yield of oil extracted was strongly affected by the reaction temperature and time. Higher reaction temperature induces the dielectric constant of water towards the non-polar properties of solvent; thus increases the oil extraction capability. Meanwhile, the highest yield of sugar was 0.20 g-sugar/g-dry EFB obtained at 220°C. At this temperature, the ion product of water is high enough to enable maximum sub-critical water hydrolysis reaction. This study showed that oil and other valuable material can be recovered using water at sub-critical condition, and most attractive without the use of harmful organic solvent.
  17. Fulltext Coupling Genotyping and Computational Modeling in Prediction of Anti-epileptic Drugs that cause Stevens Johnson Syndrome and Toxic Epidermal Necrolysis for Carrier of HLA-B*15:02
    Teh LK, Selvaraj M, Bannur Z, Ismail MI, Rafia H, Law WC, et al.
    J Pharm Pharm Sci, 2016;19(1):147-60.
    PMID: 27096699 DOI: 10.18433/J38G7X
    PURPOSE: The importance of HLA-B*15:02 genotyping to avoid carbamazepine induced SJS/TEN and molecular modeling to predict the role of HLA-B*15:0 and AEDs induced SJS/TEN are investigated.

    METHODS: DNA was extracted from eighty-six patients. The patients were genotyped by AS-PCR. Computational modeling of the HLA-B*15:02 followed by docking studies were performed to screen 26 AEDs that may induce ADR among HLA-B*15:02 carriers.

    RESULTS: Odd ratio for CBZ induced SJS/TEN and HLA-B*15:02 was 609.0 (95% CI: 23-15873; p=0.0002). Molecular modeling studies showed that acetazolamide, ethosuxiamide, lamotrigine, oxcarbazepine, phenobarbital, phenytoin, primidone and sodium-valproate may induce ADR in HLA-B*15:02 carriers alike CBZ. Conclusion. We confirmed HLA-B*15:02 as a predictor of SJS/TEN and recommend pre-screening. Computational prediction of DIHR is useful in personalized medicine.

  18. Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward
    Habib MAH, Ismail MN
    J Plant Res, 2021 Jan;134(1):43-53.
    PMID: 33108557 DOI: 10.1007/s10265-020-01231-x
    Natural rubber or latex from the Hevea brasiliensis is an important commodity in various economic sectors in today's modern society. Proteins have been detected in latex since the early twentieth century, and they are known to regulate various biological pathways within the H. brasiliensis trees such as the natural rubber biosynthesis, defence against pathogens, wound healing, and stress tolerance. However, the exact mechanisms of the pathways are still not clear. Proteomic analyses on latex have found various proteins and revealed how they fit into the mechanisms of the biological pathways. In the past three decades, there has been rapid latex protein identification due to the improvement of latex protein extraction methods, as well as the emergence of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). In this manuscript, we reviewed the methods of latex protein extraction that keeps on improving over the past three decades as well as the results of numerous latex protein identification and quantitation.
  19. Correction to: Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward
    Habib MAH, Ismail MN
    J Plant Res, 2023 May;136(3):431.
    PMID: 36943551 DOI: 10.1007/s10265-023-01451-x
  20. The detrimental effects of the COVID-19 pandemic on domestic violence against women
    Noman AHM, Griffiths MD, Pervin S, Ismail MN
    J Psychiatr Res, 2021 02;134:111-112.
    PMID: 33383493 DOI: 10.1016/j.jpsychires.2020.12.057
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