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  1. Adanan NFA, Ooi FK, Samsudin N
    Malays J Med Sci, 2024 Aug;31(4):174-184.
    PMID: 39247102 DOI: 10.21315/mjms2024.31.4.14
    BACKGROUND: Martial arts training is beneficial for improving physical fitness but the improvements can vary according to the type of martial art performed. This study investigated lung function, aerobic and anaerobic capacity, flexibility, muscular strength and power among male adolescents who were sedentary or who practiced taekwondo or wushu.

    METHODS: A total of 30 male adolescents living in the Kelantan state in Malaysia who were between 14 years old and 20 years old were enrolled and divided into three groups: i) sedentary (control), ii) taekwondo and iii) wushu. Each participant underwent a lung function test, 20 m shuttle run, Wingate anaerobic test and a Sit and Reach test, as well as tests of standing long jump power, handgrip, back and leg strength.

    RESULTS: Relative to the sedentary control group, the wushu group had significantly higher values than the sedentary control group for fat free mass (P = 0.047), explosive leg power (P < 0.001), aerobic capacity (P < 0.001), forced expiratory volume in 1 s (FEV1) (P = 0.021) and Wingate anaerobic capacity (P < 0.05). The taekwondo group also showed significantly greater values than the sedentary control group for explosive leg power (P = 0.018), forced vital capacity (FVC) (P = 0.014) and FEV1 (P < 0.001). The wushu group exhibited significantly higher explosive leg power (P = 0.010) and Wingate anaerobic capacity parameters including mean power (P = 0.001), anaerobic capacity (P < 0.001) and anaerobic power (P = 0.002) than the taekwondo group.

    CONCLUSION: Engagement of male adolescents in wushu and taekwondo was associated with improved physiological profiles compared to those who were in the sedentary control group. Practice of wushu could result in greater explosive leg power and anaerobic capacities than taekwondo.

  2. Samsudin NA, Halim NRA, Sarbon NM
    J Food Sci Technol, 2018 Nov;55(11):4608-4614.
    PMID: 30333657 DOI: 10.1007/s13197-018-3399-0
    The aim of this study is to investigate the effect of pH levels on functional properties of various molecular weights of eel (Monopterus sp.) protein hydrolysate (EPH). The eel was enzymatically hydrolyzed and fractionated through membranes filter (10 kDa, 5 kDa and 3 kDa). The foaming capacity and stability, emulsifying capacity and stability index, water holding capacity and fat binding capacity between pH 2 and 10 were determined. The 5 kDa EPH was found to have the highest foaming capacity at pH 2, pH 4 and pH 6, and foaming stability and emulsifying activity index at all pH levels, except pH 8 and fat binding capacity at pH 2, as compared to 10 kDa and 3 kDa EPH fractions. The 10 kDa EPH had the highest emulsifying stability index and water holding capacity at all pH levels. This study shows that the EPH fractions at low pH level had high foaming and oil binding capacity, while at neutral pH, the fractions had high foaming stability and water holding capacity. These properties are important in making whipped cream, mousse and meringue. In contrast, EPH fractions demonstrated strong emulsifying properties at high pH levels and show potential as an emulsifier for breads, biscuits and frozen desserts.
  3. Farawahida AH, Jinap S, Nor-Khaizura MAR, Samsudin NIP
    PMID: 28871861 DOI: 10.1080/19440049.2017.1375605
    Among the many roles played by small and medium enterprises (SMEs) in the food industry is the production of heritage foods such as peanut sauce. Unfortunately, the safety of peanut sauce is not always assured as the processing line is not controlled. Peanut sauce is usually made of peanuts and chilli, and these commodities are normally contaminated with Aspergillus spp. and aflatoxins (AFs). Hence, the objective of this study was to evaluate the practices related to reduction of AF hazard and the effect of interventions in peanut sauce processing. Peanut samples were collected from each step of peanut sauce processing from a small peanut sauce company according to four designs: (1) control; (2) oil-less frying of chilli powder; (3) addition of retort processing; and (4) combination of oil-less frying of chilli powder and retort processing. Oil-less frying of chilli powder (Design 2) reduced total AFs by 33-41%, retort processing (Design 3) reduced total AFs by 49%, while combination of these two thermal processes (Design 4) significantly reduced total AFs, by 57%. The present work demonstrated that Design 4 yielded the highest reduction of total AFs and is therefore recommended to be employed by SME companies.
  4. Samsudin N, Chua WC, Hasan H, Hassan SA, Deris ZZ
    Malays J Pathol, 2024 Apr;46(1):95-102.
    PMID: 38682849
    Borderline oxacillin-resistant Staphylococcus aureus (BORSA) are mecA-negative strains with oxacillin minimum inhibitor concentration (MIC) close to the resistance breakpoint of ≥ 4μg/mL. Instead of producing penicillin-binding protein with low affinity to methicillin (oxacillin) mediated by mecA gene as in methicillin-resistant S. aureus (MRSA), BORSA strains are characterised by the hyperproduction of β-lactamase enzymes, thus able to break down methicillin. Common laboratory methods to detect MRSA such as cefoxitin disk diffusion alone may fail to detect methicillin resistance due to BORSA. We report five cases of BORSA blood-stream infections in a university teaching hospital. All isolates were found to be susceptible to cefoxitin using disk diffusion, resistant to oxacillin using automated MIC method, and did not harbour mecA gene. All patients were suscessfully treated with anti-MRSA antibiotics, and removal of primary sources were done if identified. A more cost-effective method for screening and diagnosis of BORSA is needed in addition to cefoxitin disk diffusion test, in order to monitor the spread, and to enable routine detection and treatment of this pathogen.
  5. Salarzadeh Jenatabadi H, Bt Wan Mohamed Radzi CWJ, Samsudin N
    PMID: 32708480 DOI: 10.3390/ijerph17145201
    As postpartum obesity is becoming a global public health challenge, there is a need to apply postpartum obesity modeling to determine the indicators of postpartum obesity using an appropriate statistical technique. This research comprised two phases, namely: (i) development of a previously created postpartum obesity modeling; (ii) construction of a statistical comparison model and introduction of a better estimator for the research framework. The research model displayed the associations and interactions between the variables that were analyzed using the Structural Equation Modeling (SEM) method to determine the body mass index (BMI) levels related to postpartum obesity. The most significant correlations obtained were between BMI and other substantial variables in the SEM analysis. The research framework included two categories of data related to postpartum women: living in urban and rural areas in Iran. The SEM output with the Bayesian estimator was 81.1%, with variations in the postpartum women's BMI, which is related to their demographics, lifestyle, food intake, and mental health. Meanwhile, the variation based on SEM with partial least squares estimator was equal to 70.2%, and SEM with a maximum likelihood estimator was equal to 76.8%. On the other hand, the output of the root mean square error (RMSE), mean absolute error (MSE) and mean absolute percentage error (MPE) for the Bayesian estimator is lower than the maximum likelihood and partial least square estimators. Thus, the predicted values of the SEM with Bayesian estimator are closer to the observed value compared to maximum likelihood and partial least square. In conclusion, the higher values of R-square and lower values of MPE, RMSE, and MSE will produce better goodness of fit for SEM with Bayesian estimators.
  6. Chen X, Bailey RP, Yin X, Samsudin N
    Front Psychol, 2024;15:1416783.
    PMID: 39539303 DOI: 10.3389/fpsyg.2024.1416783
    OBJECTIVE: This study examines the relationship between Teacher-Student Relationships and academic grades among Chinese rural high school students, focusing on the moderating role of mental health symptoms and the conditional moderating effect of academic resilience.

    METHOD: A moderated moderation analysis was conducted via Mplus on data collected from a sample of rural Chinese high school students. SEM was used to test the direct and interactive effects of these variables on academic outcomes.

    RESULTS: Teacher-Student Relationships were found to have a significant positive association with students' academic grades. Academic resilience plays a conditional moderating role, with students who have higher levels of resilience better able to maintain their academic performance, even when facing psychological distress. This suggests that resilience can buffer the impact of challenges, enhancing the positive influence of TSRs on academic outcomes.

  7. Wan Mohamed Radzi CWJB, Salarzadeh Jenatabadi H, Samsudin N
    BMC Public Health, 2021 01 27;21(1):27.
    PMID: 33499833 DOI: 10.1186/s12889-020-09999-2
    BACKGROUND: Since the last decade, postpartum depression (PPD) has been recognized as a significant public health problem, and several factors have been linked to PPD. Mothers at risk are rarely undetected and underdiagnosed. Our study aims to determine the factors leading to symptoms of depression using Structural Equation Modeling (SEM) analysis. In this research, we introduced a new framework for postpartum depression modeling for women.

    METHODS: We structured the model of this research to take into consideration the Malaysian culture in particular. A total of 387 postpartum women have completed the questionnaire. The symptoms of postpartum depression were examined using the Edinburgh Postnatal Depression Scale (EPDS), and they act as a dependent variable in this research model.

    RESULTS: Four hundred fifty mothers were invited to participate in this research. 86% of the total distributed questionnaire received feedback. The majority of 79.6% of respondents were having depression symptoms. The highest coefficients of factor loading analysis obtained in every latent variable indicator were income (β = 0.77), screen time (β = 0.83), chips (β = 0.85), and anxiety (β = 0.88). Lifestyle, unhealthy food, and BMI variables were directly affected by the dependent variable. Based on the output, respondents with a high level of depression symptoms tended to consume more unhealthy food and had a high level of body mass indexes (BMI). The highest significant impact on depression level among postpartum women was unhealthy food consumption. Based on our model, the findings indicated that 76% of the variances stemmed from a variety of factors: socio-demographics, lifestyle, healthy food, unhealthy food, and BMI. The strength of the exogenous and endogenous variables in this research framework is strong.

    CONCLUSION: The prevalence of postpartum women with depression symptoms in this study is considerably high. It is, therefore, imperative that postpartum women seek medical help to prevent postpartum depressive symptoms from worsening.

  8. Wan Abdul Rahman WM, Saddki N, Mahmood Z, Hasan R, Samsudin NA
    Med J Malaysia, 2024 Jan;79(1):1-8.
    PMID: 38287750
    INTRODUCTION: Women's important roles within families which include modelling appropriate oral health behaviours require them to have good knowledge and positive attitude in oral health. This study determined knowledge and attitude towards children's oral health among first-time mothers and factors associated with the attributes.

    MATERIALS AND METHODS: A total of 154 first-time mothers in the third trimester of pregnancy who attended two health clinics in the state of Sarawak, Malaysia for antenatal care participated in this cross-sectional study. A structured selfadministered questionnaire was used to measure the variables of interest.

    RESULTS: Most mothers could correctly identify the aetiological factors of dental caries and strategies for preventing the disease in children. However, a substantial portion could not identify certain cariogenic and noncariogenic foods or drinks. Most pregnant women have appropriate attitudes towards children's oral health although some showed unfavourable attitude about care of primary teeth. Women who were older and had attended a talk on children's oral health were more likely to have higher mean knowledge score than their respective counterparts, and higher mean knowledge score was associated with higher mean attitude score.

    CONCLUSION: Most first-time mothers in this study had correct knowledge and favourable attitude about children's oral health, although misunderstandings and misperceptions in several issues were also common. Significant association found between experience of attending oral health talk and oral health knowledge, and between oral health knowledge and attitude, substantiate the importance of an educational intervention program to optimise the mothers' roles in caries prevention in children.

  9. Samsudin N, Noordin R, Anuar NS, Rahumatullah A, Abdullah ADG, Husin A, et al.
    Trop Biomed, 2024 Dec 01;41(4):422-426.
    PMID: 39876498 DOI: 10.47665/tb.41.4.001
    Strongyloidiasis is a parasitic nematode infection mainly caused by Strongyloides stercoralis. Immunocompromised conditions, particularly cancer patients treated with chemotherapy and corticosteroids, have a significant risk of developing Strongyloides hyperinfection. The lack of a gold standard laboratory method to rule out this infection and the insensitivity of microscopic stool examination due to low and intermittent larvae output in stool contribute to the low detection rate of this infection. We present three cases of strongyloidiasis in adults with haematological malignancies and significant eosinophilia in the early course of their cancer. Two patients were diagnosed with a combination of serological and molecular tests, and one was diagnosed serologically. Ivermectin at 200 mcg/kg/day for two days was commenced for all patients; unfortunately, one patient succumbed.
  10. Arushothy R, Ahmad N, Amran F, Hashim R, Samsudin N, Azih CRC
    Int J Infect Dis, 2019 Mar;80:129-133.
    PMID: 30572022 DOI: 10.1016/j.ijid.2018.12.009
    OBJECTIVE: This study was performed to analyze the serotype distribution of Streptococcus pneumoniae causing invasive pneumococcal disease (IPD) in children aged 5 years and under in Malaysia and to assess the antimicrobial resistance.

    METHODS: From 2014 to 2017, a total of 245 invasive S. pneumoniae isolates from children ≤5 years of age were received from hospitals all around Malaysia. All isolates were identified and subjected to serotyping and antimicrobial susceptibility testing.

    RESULTS: Of the 245 isolates, 117 (48.0%) were from children aged <1year, 46 (19.05%) were from children aged 1-2 years, and 82 (33.0%) were from children aged 2-5 years. The most common serotypes were 14 (26.9%), 6B (19.6%), 19A (11.8%), 6A (10.6%), and 19F (6.9%) and vaccine coverage was 88.2% for PCV13, 64.1% for PCV10, and 63.3% for PCV7. Resistance to penicillin was 0.2% for non-meningitis cases and 22.2% for meningitis cases; erythromycin resistance was reported in 42.9%, co-trimoxazole in 35.9%, and tetracycline in 42.9%.

    CONCLUSIONS: Serotypes 14, 6B, 19A, 6A, and 19F were the most common serotypes isolated from children with IPD in Malaysia during this pre-vaccination era. The lack of reports on the serotype distribution has limited action for the implementation of PCV in the national immunization programme (NIP). The information from this study may benefit future policies for the introduction of PCV in the Malaysian NIP and ultimately may reduce the morbidity and mortality among children in Malaysia.

  11. Peter Mshelia L, Selamat J, Iskandar Putra Samsudin N, Rafii MY, Abdul Mutalib NA, Nordin N, et al.
    Toxins (Basel), 2020 07 28;12(8).
    PMID: 32731333 DOI: 10.3390/toxins12080478
    Climate change is primarily manifested by elevated temperature and carbon dioxide (CO2) levels and is projected to provide suitable cultivation grounds for pests and pathogens in the otherwise unsuitable regions. The impacts of climate change have been predicted in many parts of the world, which could threaten global food safety and food security. The aim of the present work was therefore to examine the interacting effects of water activity (aw) (0.92, 0.95, 0.98 aw), CO2 (400, 800, 1200 ppm) and temperature (30, 35 °C and 30, 33 °C for Fusarium verticillioides and F. graminearum, respectively) on fungal growth and mycotoxin production of acclimatised isolates of F. verticillioides and F. graminearum isolated from maize. To determine fungal growth, the colony diameters were measured on days 1, 3, 5, and 7. The mycotoxins produced were quantified using a quadrupole-time-of-flight mass spectrometer (QTOF-MS) combined with ultra-high-performance liquid chromatography (UHPLC) system. For F. verticillioides, the optimum conditions for growth of fumonisin B1 (FB1), and fumonisin B2 (FB2) were 30 °C + 0.98 aw + 400 ppm CO2. These conditions were also optimum for F. graminearum growth, and zearalenone (ZEA) and deoxynivalenol (DON) production. Since 30 °C and 400 ppm CO2 were the baseline treatments, it was hence concluded that the elevated temperature and CO2 levels tested did not seem to significantly impact fungal growth and mycotoxin production of acclimatised Fusarium isolates. To the best of our knowledge thus far, the present work described for the first time the effects of simulated climate change conditions on fungal growth and mycotoxin production of acclimatised isolates of F. verticillioides and F. graminearum.
  12. Samsudin N, Hashim YZH, Arifin MA, Mel M, Salleh HM, Sopyan I, et al.
    Cytotechnology, 2017 Aug;69(4):601-616.
    PMID: 28337561 DOI: 10.1007/s10616-017-0071-x
    Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 µg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93°-49.34°) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL.
  13. Arifin MA, Mel M, Swan SY, Samsudin N, Hashim YZH, Salleh HM
    Prep Biochem Biotechnol, 2022;52(2):181-196.
    PMID: 34010098 DOI: 10.1080/10826068.2021.1923031
    The aim of this study was to develop gelatin coated polystyrene (PS) microcarriers with good cell adhesion and proliferation properties. PS microspheres, prepared using oil-in water (o/w) solvent evaporation method, were loaded with oxygen containing functional groups using an ultraviolet/ozone (UVO3) system. Using water-soluble carbodiimide chemistry, gelatin was subsequently immobilized on UVO3 treated PS microspheres. The amount of immobilized gelatin was found to be directly proportional to the surface carboxyl (COOH) concentration on PS microspheres. Face Centered Central Composite Design (FCCD) was employed to optimize the process conditions of UVO3 treatment to maximize the surface COOH concentration on PS microspheres for allowing higher gelatin immobilization. Statistical results revealed that, the optimized process conditions were ozone flow rate of ∼64,603 ppm, exposure time of ∼60 minutes and sample amount of 5.05 g. Under these conditions, the surface COOH concentration on PS microspheres was ∼1,505 nmol/g with the corresponding amount of immobilized gelatin was ∼2,725 µg/g. Characterization analyses strongly suggest that the optimized UVO3 treatment and successive gelatin immobilization have successfully improved surface wettability and dispersion stability of PS microspheres. Moreover, gelatin coated PS microcarriers were also proven as able to support the growth of CHO-K1 cells in high cell density culture.
  14. Mohamed NA, Alanzi ARA, Azizan NA, Azizan SA, Samsudin N, Jenatabadi HS
    Front Psychol, 2023;14:1060963.
    PMID: 36910750 DOI: 10.3389/fpsyg.2023.1060963
    INTRODUCTION: Depression and obesity are the main threat among women which have been considered by many research scholars in psychology studies. In their analysis for measuring and estimating obesity and depression they were involving statistical functions.

    METHODS: Regression, Analysis of Variance (ANOVA), and in the last two decades Structural Equation Modeling are the most familiar statistical methods among research scholars. Taguchi algorism process is one the statistical methods which mostly have been applying in engineering studies. In this study we are looking at two main objectives. The first one is to introduce Taguchi algorism process and apply it in a case study in psychology area. The second objective is challenging among four statistical techniques include ANOVA, regression, SEM, and Taguchi technique in a same data. To achieve those aims we involved depression and obesity indices with other familiar indicators contain socioeconomic, screen time, sleep time, and usage fitness and nutrition mobile applications.

    RESULTS AND DISCUSSION: Outputs proved that Taguchi technique is able to analyze some correlations which are not achieved by applying ANOVA, regression, and SEM. Moreover, SEM has a special capability to estimate some hidden correlations which are not possible to evaluate them by using ANOVA, regression, and even Taguchi method. In the last, we found that some correlations are significant by SEM, however, in the same data with regression those correlation were not significant. This paper could be a warning for psychology research scholars to be more careful with involving statistical methods for measuring and estimating of their research variables.

  15. Samsudin N, Bailey RP, Ries F, Hashim SNAB, Fernandez JA
    BMC Sports Sci Med Rehabil, 2024 May 08;16(1):107.
    PMID: 38720395 DOI: 10.1186/s13102-024-00895-5
    CONTEXT: The prevalence of depression among teenagers is a significant issue worldwide, which calls for a thorough investigation of non-drug treatments. This expedited evaluation examines 24 specifically chosen studies to clarify the correlation between physical activity depression symptoms in teenagers, undertaken following PRISMA principles.

    METHODS: A wide range of research methods, including longitudinal studies, surveys, and cross-sectional analyses, were used in different nations to understand the intricate relationship between physical activity, sedentary behaviours, and depression symptoms. The data-gathering methods included standardised questionnaires, accelerometer measurements, and self-report instruments.

    FINDINGS: The review highlights the crucial significance of engaging in physical activity to alleviate depression symptoms. Improved self-esteem consistently acts as a crucial intermediary between participation in physical activity and decreased rates of depression. Engaging in physical activity is a safeguard, particularly for individuals with restricted access to physical activity. In contrast, a sedentary lifestyle greatly increases the probability of developing moderate to severe symptoms of depression. Gender differences are apparent, with females being disproportionately impacted by depression. There are strong connections between engaging in physical activity and reducing symptoms of depression, which can be observed in various situations, such as participating in team sports or engaging in leisure activities.

    CONCLUSION: This study provides insight into the potential of physical activity as a non-pharmacological approach to address adolescent depression. This highlights the significant impact of physical activity, which has important implications for public health programs aimed at improving the mental well-being of adolescents by promoting physical activity. It is crucial to do additional research that considers gender-specific variations and various physical activity circumstances to enhance our comprehension of this important matter.

  16. Othman MK, Yusof Z, Hussin SA, Samsudin N, Muhd Besari AB, W Isa WYH
    JACC Case Rep, 2022 Oct 05;4(19):1288-1291.
    PMID: 36406916 DOI: 10.1016/j.jaccas.2022.07.016
    We present a previously healthy young man with cardiac tamponade. He underwent emergency pericardiocentesis. The pericardial fluid was exudative, and Salmonella sp. was grown on both pericardial and blood cultures. Further investigations revealed that this patient had classical Hodgkin lymphoma, which explains his immunocompromised state predisposing him to this infection. (Level of Difficulty: Advanced.).
  17. Shengyao Y, Xuefen L, Jenatabadi HS, Samsudin N, Chunchun K, Ishak Z
    BMC Psychol, 2024 Jul 12;12(1):389.
    PMID: 38997786 DOI: 10.1186/s40359-024-01886-4
    The main objective of this study is to examine the relationship of emotional intelligence with psychological well-being and academic achievement through positive psychological characteristics among university students in China. The study was conducted with postgraduate and undergraduate students. The integration of emotional intelligence theory and positive psychological theory was used in this study. The introduced framework included emotional intelligence as the main independent variable, self-efficacy, motivation, and resilience as three mediators, and psychological well-being and academic achievement as two dependent variables. A survey was conducted among 518 students, and structural equation modelling was used to analyse the data. The study found that emotional intelligence was positively related to positive psychological characteristics, psychological well-being, and academic achievement, and the effects were stronger among postgraduate students. Also, positive psychological characteristics, which include self-efficacy, motivation, and resilience, mediate the relationship between emotional intelligence and psychological well-being and academic achievement, and the relationship was stronger among postgraduate students. Proper coping strategies and mechanisms can be helpful to improve both psychological well-being and academic achievement at the same time among university students.
  18. Mohamed NA, Alanzi ARA, Azizan AN, Azizan SA, Samsudin N, Salarzadeh Jenatabadi H
    PLoS One, 2024;19(1):e0290376.
    PMID: 38261595 DOI: 10.1371/journal.pone.0290376
    Sustainable construction and demolition waste management relies heavily on the attitudes and actions of its constituents; nevertheless, deep analysis for introducing the best estimator is rarely attained. The main objective of this study is to perform a comparison analysis among different approaches of Structural Equation Modeling (SEM) in Construction and Demolition Waste Management (C&DWM) modeling based on an Extended Theory of Planned Behaviour (Extended TPB). The introduced research model includes twelve latent variables, six independent variables, one mediator, three control variables, and one dependent variable. Maximum likelihood (ML), partial least square (PLS), and Bayesian estimators were considered in this study. The output of SEM with the Bayesian estimator was 85.8%, and among effectiveness of six main variables on C&DWM Behavioral (Depenmalaydent variables), five of them have significant relations. Meanwhile, the variation based on SEM with ML estimator was equal to 78.2%, and four correlations with dependent variable have significant relationship. At the conclusion, the R-square of SEM with the PLS estimator was equivalent to 73.4% and three correlations with the dependent variable had significant relationships. At the same time, the values of the three statistical indices include root mean square error (RMSE), mean absolute percentage error (MPE), and mean absolute error (MSE) with involving Bayesian estimator are lower than both ML and PLS estimators. Therefore, compared to both PLS and ML, the predicted values of the Bayesian estimator are closer to the observed values. The lower values of MPE, RMSE, and MSE and the higher values of R-square will generate better goodness of fit for SEM with a Bayesian estimator. Moreover, the SEM with a Bayesian estimator revealed better data fit than both the PLS and ML estimators. The pattern shows that the relationship between research variables can change with different estimators. Hence, researchers using the SEM technique must carefully consider the primary estimator for their data analysis. The precaution is necessary because higher error means different regression coefficients in the research model.
  19. Chahil JK, Munretnam K, Samsudin N, Lye SH, Hashim NA, Ramzi NH, et al.
    Indian J Clin Biochem, 2015 Apr;30(2):134-9.
    PMID: 25883419 DOI: 10.1007/s12291-013-0414-0
    Genome-wide association studies have discovered multiple single nucleotide polymorphisms (SNPs) associated with the risk of common diseases. The objective of this study was to demonstrate the replication of previously published SNPs that showed statistical significance for breast cancer in the Malaysian population. In this case-control study, 80 subjects for each group were recruited from various hospitals in Malaysia. A total of 768 SNPs were genotyped and analyzed to distinguish risk and protective alleles. A total of three SNPs were found to be associated with increased risk of breast cancer while six SNPs showed protective effect. All nine were statistically significant SNPs (p ≤ 0.01), five SNPs from previous studies were successfully replicated in our study. Significant modifiable (diet) and non-modifiable (family history of breast cancer in first degree relative) risk factors were also observed. We identified nine SNPs from this study to be either conferring susceptibility or protection to breast cancer which may serve as potential markers in risk prediction.
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