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  1. Cold-pressed rapeseed (Brassica napus) oil: Chemistry and functionality
    Chew SC
    Food Res Int, 2020 05;131:108997.
    PMID: 32247493 DOI: 10.1016/j.foodres.2020.108997
    Rapeseed oil is the second most abundant produced edible oil in the world with low erucic acid and low glucosinolate. Thus, the quality of rapeseed oil had attracted global attention. Cold-pressed rapeseed oil appeared to be a preferred choice than refined oil as no solvent and less processing involved in the cold-pressing. The methods of cold-pressing and microwave pre-treatment on the extraction yield and bioactive compounds of rapeseed oil have been reviewed in this paper. Cold-pressed rapeseed oil offers health benefits due to its preserved fatty acid profile and bioactive compounds. High phenolic compounds, tocopherols, phytosterols, and carotenoids contents in the cold-pressed rapeseed oil offer health benefits like regulating blood lipid profile, insulin sensitivity, and glycemic control, as well as offer antioxidant and cytotoxic activity. Besides using as edible oil, cold-pressed rapeseed oil find applications in animal feed, chemical, and fuel.
    Matched MeSH terms: Brassica napus/chemistry*
  2. The effects of taste sensitivity and repeated taste exposure on children's intake and liking of turnip (Brassica rapa subsp. rapa); a bitter Brassica vegetable
    Mohd Nor ND, Houston-Price C, Harvey K, Methven L
    Appetite, 2021 02 01;157:104991.
    PMID: 33049340 DOI: 10.1016/j.appet.2020.104991
    Low consumption of vegetables in children is a concern around the world, hence approaches aimed at increasing intake are highly relevant. Previous studies have shown that repeated taste exposure is an effective strategy to increase vegetable acceptance. However, few studies have examined the effect of repeated taste exposure on children varying in bitter taste sensitivity. This study investigated the influence of taste genotypes and phenotypes on the effects of repeated taste exposure to a Brassica vegetable. 172 preschool children aged 3-5 years were recruited into this study. Turnip was selected as the target vegetable and parents completed a questionnaire to ensure unfamiliarity. During the intervention, children were exposed to steamed-pureed turnip for 10 days (once/day). Intake and liking were measured before, during and after the intervention, and a follow-up was done 3 months post-intervention. Taste genotypes (TAS2R38 and gustin (CA6) genotypes) and taste phenotypes (PROP taster status and fungiform papillae density) were determined. There was a significant effect of exposure shown by significant increases in intake (p 
    Matched MeSH terms: Brassica napus*
  3. Identification of a potential structural marker for embryogenic competency in the Brassica napus spp. oleifera embryogenic tissue
    Namasivayam P, Skepper J, Hanke D
    Plant Cell Rep, 2006 Sep;25(9):887-95.
    PMID: 16568254
    The Brassica napus secondary embryogenesis system requires no exogenous growth regulator to stimulate embryo development. It is stable embryogenically over a long period of culture and has a distinct pre-embryogenic stage. This system was used to investigate the morphological and cellular changes occurring in the embryogenic tissue compared to non-embryogenic tissue using various microscopy techniques. A unique ultrastructural feature designated the extracellular matrix (ECM) was observed on the surface of pre-embryogenic embryoids but not on the non-embryogenic individuals. The ECM layer was found to be dominant in the pre-embryogenic stage and reduced to fragments during embryo growth and development in mature embryogenic tissue. This is a novel aspect of the phenotype previously unreported in the Brassica system. This structure might be linked to acquisition of embryogenic competence.
    Matched MeSH terms: Brassica napus/classification; Brassica napus/embryology*; Brassica napus/ultrastructure*
  4. Fulltext Speed breeding is a powerful tool to accelerate crop research and breeding
    Watson A, Ghosh S, Williams MJ, Cuddy WS, Simmonds J, Rey MD, et al.
    Nat Plants, 2018 Jan;4(1):23-29.
    PMID: 29292376 DOI: 10.1038/s41477-017-0083-8
    The growing human population and a changing environment have raised significant concern for global food security, with the current improvement rate of several important crops inadequate to meet future demand 1 . This slow improvement rate is attributed partly to the long generation times of crop plants. Here, we present a method called 'speed breeding', which greatly shortens generation time and accelerates breeding and research programmes. Speed breeding can be used to achieve up to 6 generations per year for spring wheat (Triticum aestivum), durum wheat (T. durum), barley (Hordeum vulgare), chickpea (Cicer arietinum) and pea (Pisum sativum), and 4 generations for canola (Brassica napus), instead of 2-3 under normal glasshouse conditions. We demonstrate that speed breeding in fully enclosed, controlled-environment growth chambers can accelerate plant development for research purposes, including phenotyping of adult plant traits, mutant studies and transformation. The use of supplemental lighting in a glasshouse environment allows rapid generation cycling through single seed descent (SSD) and potential for adaptation to larger-scale crop improvement programs. Cost saving through light-emitting diode (LED) supplemental lighting is also outlined. We envisage great potential for integrating speed breeding with other modern crop breeding technologies, including high-throughput genotyping, genome editing and genomic selection, accelerating the rate of crop improvement.
    Matched MeSH terms: Brassica napus/genetics*
  5. A simple and high efficiency purification of His-tagged turnip yellow mosaic virus-like particle (TYMV-VLP) by nickel ion affinity precipitation
    Tan FH, Ng JF, Mohamed Alitheen NB, Muhamad A, Yong CY, Lee KW
    J Virol Methods, 2023 Sep;319:114771.
    PMID: 37437780 DOI: 10.1016/j.jviromet.2023.114771
    Virus-like particles (VLPs) is one of the most favourable subjects of study, especially in the field of nanobiotechnology and vaccine development because they possess good immunogenicity and self-adjuvant properties. Conventionally, VLPs can be tagged and purified using affinity chromatography or density gradient ultracentrifugation which is costly and time-consuming. Turnip yellow mosaic virus (TYMV) is a plant virus, where expression of the viral coat protein (TYMVc) in Escherichia coli (E. coli) has been shown to form VLP. In this study, we report a non-chromatographic method for VLP purification using C-terminally His-tagged TYMVc (TYMVcHis6) as a protein model. Firstly, the TYMVcHis6 was cloned and expressed in E. coli. Upon clarification of cell lysate, nickel (II) chloride [NiCl2; 15 µM or equivalent to 0.0000194% (w/v)] was added to precipitate TYMVcHis6. Following centrifugation, the pellet was resuspended in buffer containing 1 mM EDTA to chelate Ni2+, which is then removed via dialysis. A total of 50% of TYMVcHis6 was successfully recovered with purity above 0.90. Later, the purified TYMVcHis6 was analysed with sucrose density ultracentrifugation, dynamic light scattering (DLS), and transmission electron microscopy (TEM) to confirm VLP formation, which is comparable to TYMVcHis6 purified using the standard immobilized metal affinity chromatography (IMAC) column. As the current method omitted the need for IMAC column and beads while significantly reducing the time needed for column washing, nickel affinity precipitation represents a novel method for the purification of VLPs displaying poly-histidine tags (His-tags).
    Matched MeSH terms: Brassica napus*
  6. Fulltext The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)
    Ton LB, Neik TX, Batley J
    Genes (Basel), 2020 09 30;11(10).
    PMID: 33008008 DOI: 10.3390/genes11101161
    Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.
    Matched MeSH terms: Brassica napus/genetics*; Brassica napus/growth & development; Brassica napus/physiology
  7. Fulltext Physicochemical properties of canola oil, olive oil and palm olein blends
    Roiaini, M., Ardiannie, T., Norhayai, H.
    International Food Research Journal, 2015;22(3):1227-1233.
    MyJurnal
    Oil blending has been recognized as one of the most potent solution in producing vegetable oils with good storage stabilities and optimum fatty acids compositions. This study was conducted to identify the best oil blends in terms of physicochemical properties between canola, olive and palm olein oil. Canola and olive oils were blended at different ratios of 80:20, 60:40, 50:50, 40:60, and 20:80. Palm olein is stable against rancidity and oxidation thus the above blends were mixed with 20% palm olein. The 80:20 canola: olive blend shows the best properties after being mixed with 20% palm olein compared to other blends. It passed the cold stability test and has significantly the lowest cloud point of 6.0oC (p
    Matched MeSH terms: Brassica napus
  8. Fulltext Comparison of melting behaviors of edible oils using conventional and hyper differential scanning calorimetric scan rates
    Abdulkarim, S.M., Ghazali, H.M.
    International Food Research Journal, 2007;14(1):25-35.
    MyJurnal
    HyperDSC™(fast scan rate) was used to study the melting behavior of canola (CLO), sunflower (SFO), palm olein (PO), rice bran oils (RBO), and cocoa butter (CB), and was compared to the melting behaviors using conventional DSC. There was an increase in sensitivity with increase in scan rate. Slow scan rate (5 to 20C/min) gave low sensitivity, which increased when the scan rates were increased to 50, 100 and 200C/min. Peak resolution was affected by scan rate depending on the sample weight. Increase in the size of sample coupled with the use of fast scan rate decreased the peak resolution. Generally small sample sizes gave better peak resolution. Results of the effect of scan rate on glass transition (Tg) shows that Tg, which is a weak transition especially in crystalline and low amorphous materials was not detected using conventional scan rates (5 to 20oC/min). It was however detected using of hyperDSC™ scan rates (100 to 200oC/min). Increasing the scan rate resulted in an increase in the peak temperature and the elimination of shoulder peaks, which were caused due to the polymorphic behavior of the triacylglycerols in the oils. The increase in peak temperature caused a shift in the peak position towards a higher temperature value. There is a positive correlation between the peak temperature and scan rate. The correlation coefficients (r) for CLO, SFO, PO, RBO and CB were 0.96, 0.95, 0.97, 0.96 and 0.96 respectively.
    Matched MeSH terms: Brassica napus
  9. Effect of Zeolite and Zinc on the biochemical characteristics of canola upon drought stress
    Nasser Shahsavari, Hasnah Mohd. Jais, Amir Hossein Shirani Rad
    Sains Malaysiana, 2014;43:1549-1555.
    The effects of zeolite and zinc foliar applications on the biochemical characteristics of canola cultivars under different moisture regimes were investigated in a study conducted during the 2010 and 2011 growing seasons. The study was completed using a factorial split-plot experiment based on randomized complete block design (RCBD) with three replications at the Seed and Plant Improvement Institute (SPII), Karaj, Iran. The treatments were: irrigation (I): complete (I1) and restricted (I2); zeolite (Z): 0 (Z1) and 15 ton ha-1 (Z2) and Zn: 0, 0.1 and 0.2% concentrations of zinc sulfate (Zn1, Zn2, and Zn3) at the pod formation stage. These treatments were applied during the pod formation stage to the Licord, RGS003 and Opera cultivars. This study showed that although applying Z and Zn had positive effects on the quality of canola, the highest performance and the best results were obtained using a combination of Z and Zn. The combined application of Z and Zn decreased the proline and carbohydrate contents to 44.35 and 34.42%, respectively. Therefore, with the low cost of natural Z and moderate Zn intake, these treatments can be used to enhance the performance of canola, especially in regions frequently subjected to water stress.
    Matched MeSH terms: Brassica napus
  10. Understanding of the Role of Pretreatment Methods on Rapeseed Oil from the Perspective of Phenolic Compounds
    Xu YJ, Jiang F, Song J, Yang X, Shu N, Yuan L, et al.
    J Agric Food Chem, 2020 Aug 19;68(33):8847-8854.
    PMID: 32806128 DOI: 10.1021/acs.jafc.0c03539
    The thermal pretreatment of oilseed prior to oil extraction could increase the oil yield and improve the oil quality. Phenolic compounds are important antioxidants in rapeseed oil. In this study, we investigated the impact of thermal pretreatment method on the rapeseed oil based on phenolic compound levels. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) analysis showed that the phenolic compound contents in the microwave-pretreated oil were higher than those in the oven- and infrared-treated oils. Sinapic acid (SA) and canolol (CA), which are the top two phenolic compounds in rapeseed oil, exerted well 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with IC50 values of 8.45 and 8.80 μmol/L. The cell experiment uncovered that SA and CA have significant biological activities related to rapeseed oil quality, including increase of antioxidant enzymes superoxide dismutase (SOD), alleviation of reactive oxygen species (ROS), and cytotoxicity of HepG2 cells after the intake of excessive oleic acid. Further investigation indicated that SA and CA reduced cell apoptosis rate through Bax-Bcl-2-caspase-3 and p53-Bax-Bcl-2-caspase-3, respectively. Taken together, our findings suggest that microwave pretreatment is the best method to improve the content of phenolic compounds in rapeseed oil compared with oven and infrared pretreatments.
    Matched MeSH terms: Brassica napus/chemistry*
  11. Fulltext Understanding Host-Pathogen Interactions in Brassica napus in the Omics Era
    Neik TX, Amas J, Barbetti M, Edwards D, Batley J
    Plants (Basel), 2020 Oct 10;9(10).
    PMID: 33050509 DOI: 10.3390/plants9101336
    Brassica napus (canola/oilseed rape/rapeseed) is an economically important crop, mostly found in temperate and sub-tropical regions, that is cultivated widely for its edible oil. Major diseases of Brassica crops such as Blackleg, Clubroot, Sclerotinia Stem Rot, Downy Mildew, Alternaria Leaf Spot and White Rust have caused significant yield and economic losses in rapeseed-producing countries worldwide, exacerbated by global climate change, and, if not remedied effectively, will threaten global food security. To gain further insights into the host-pathogen interactions in relation to Brassica diseases, it is critical that we review current knowledge in this area and discuss how omics technologies can offer promising results and help to push boundaries in our understanding of the resistance mechanisms. Omics technologies, such as genomics, proteomics, transcriptomics and metabolomics approaches, allow us to understand the host and pathogen, as well as the interaction between the two species at a deeper level. With these integrated data in multi-omics and systems biology, we are able to breed high-quality disease-resistant Brassica crops in a more holistic, targeted and accurate way.
    Matched MeSH terms: Brassica napus
  12. Phytoaccumulation of heavy metals and protein expression by different vegetables collected from various parts of Khyber Pukhtunkhawa Province, Pakistan
    Jehan Bakht, Laleena Khan, Mohammad Shafi
    Sains Malaysiana, 2016;45:167-176.
    The present study investigates heavy metal uptake and protein expression by different vegetables collected from various districts of Khyber Pakhtunkhawa province of Pakistan. Statistical analysis of the data showed that maximum concentration of Cd, Cr, Ni, Zn were found in radish and spinach, respectively, collected from Peshawar. Maximum Pb and Mg accumulation were found in cauliflower and pea at Swat followed by coriander at Haripur and minimum Pb uptake was noticed in radish taken from Nowshehra. Highest Cu uptake was detected in spinach at Nowshehra. Data regarding Cd, Cr, Pb, Cu, Ni, Mg and Zn concentration in water samples gathered from different sites of KPK indicated that maximum concentration of Cd was observed in Swat. Maximum Cr and Cu concentration were measured in water samples from Peshawar while maximum concentration of Pb and Ni were detected in water samples from Haripur. In case of soil samples, maximum Cd, Cr, Mg and Zn uptake was observed in soil sample at Nowshehra. Maximum Pb and Ni concentration was found in soil samples collected from Peshawar. Cu concentration was observed to be the highest in soil at Swat. Protein profile of different vegetables i.e. cauliflower, radish, carrot, turnip, pea, spinach, coriander and garlic sampled across five different sites showed that uptake of Cd, Cr, Pb, Cu, Ni, Mg and Zn by these vegetables caused the expression of numerous polypeptides.
    Matched MeSH terms: Brassica napus
  13. Fulltext Comparative transcriptome analysis of leaves and roots in response to sudden increase in salinity in Brassica napus by RNA-seq
    Yong HY, Zou Z, Kok EP, Kwan BH, Chow K, Nasu S, et al.
    Biomed Res Int, 2014;2014:467395.
    PMID: 25177691 DOI: 10.1155/2014/467395
    Amphidiploid species in the Brassicaceae family, such as Brassica napus, are more tolerant to environmental stress than their diploid ancestors.A relatively salt tolerant B. napus line, N119, identified in our previous study, was used. N119 maintained lower Na(+) content, and Na(+)/K(+) and Na(+)/Ca(2+) ratios in the leaves than a susceptible line. The transcriptome profiles of both the leaves and the roots 1 h and 12 h after stress were investigated. De novo assembly of individual transcriptome followed by sequence clustering yielded 161,537 nonredundant sequences. A total of 14,719 transcripts were differentially expressed in either organs at either time points. GO and KO enrichment analyses indicated that the same 49 GO terms and seven KO terms were, respectively, overrepresented in upregulated transcripts in both organs at 1 h after stress. Certain overrepresented GO term of genes upregulated at 1 h after stress in the leaves became overrepresented in genes downregulated at 12 h. A total of 582 transcription factors and 438 transporter genes were differentially regulated in both organs in response to salt shock. The transcriptome depicting gene network in the leaves and the roots regulated by salt shock provides valuable information on salt resistance genes for future application to crop improvement.
    Matched MeSH terms: Brassica napus/physiology*
  14. Membrane-bound Δ12 fatty acid desaturase (FAD12); From Brassica napus to E. coli expression system
    Halim NFAA, Ali MSM, Leow ATC, Rahman RNZRA
    Int J Biol Macromol, 2021 Jun 01;180:242-251.
    PMID: 33737181 DOI: 10.1016/j.ijbiomac.2021.03.072
    Fatty acid desaturase catalyzes the desaturation reactions by insertion of double bonds into the fatty acyl chain, producing unsaturated fatty acids. Though soluble fatty acid desaturases have been studied widely in advanced organisms, there are very limited studies of membrane fatty acid desaturases due to the difficulty of generating recombinant desaturase. Brassica napus is a rapeseed, which possesses a range of different membrane-bound desaturases capable of producing fatty acids including Δ3, Δ4, Δ8, Δ9, Δ12, and Δ15 fatty acids. The 1155 bp open reading frame of Δ12 fatty acid desaturase (FAD12) from Brassica napus codes for 383 amino acid residues with a molecular weight of 44 kDa. It was expressed in Escherichia coli at 37 °C in soluble and insoluble forms when induced with 0.5 mM IPTG. Soluble FAD12 has been purified using Ni2+-Sepharose affinity chromatography with a total protein yield of 0.728 mg/mL. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that desaturase activity of FAD12 could produce linoleic acid from oleic acid at a retention time of 17.6 with a conversion rate of 47%. Characterization of purified FAD12 revealed the optimal temperature of FAD12 was 50 °C with 2 mM preferred substrate concentration of oleic acid. Analysis of circular dichroism (CD) showed FAD12 was made up of 47.3% and 0.9% of alpha-helix and β-sheet secondary structures. The predicted Tm value was 50.2 °C.
    Matched MeSH terms: Brassica napus
  15. Fulltext Therapeutic roles of natural remedies in combating hereditary ataxia: A systematic review
    Phang MWL, Lew SY, Chung I, Lim WK, Lim LW, Wong KH
    Chin Med, 2021 Jan 28;16(1):15.
    PMID: 33509239 DOI: 10.1186/s13020-020-00414-x
    BACKGROUND: Hereditary ataxia (HA) represents a group of genetically heterogeneous neurodegenerative diseases caused by dysfunction of the cerebellum or disruption of the connection between the cerebellum and other areas of the central nervous system. Phenotypic manifestation of HA includes unsteadiness of stance and gait, dysarthria, nystagmus, dysmetria and complaints of clumsiness. There are no specific treatments for HA. Management strategies provide supportive treatment to reduce symptoms.

    OBJECTIVES: This systematic review aimed to identify, evaluate and summarise the published literature on the therapeutic roles of natural remedies in the treatment of HA to provide evidence for clinical practice.

    METHODS: A systematic literature search was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Web of Science, PubMed and Science Direct Scopus were thoroughly searched for relevant published articles from June 2007 to July 2020.

    RESULTS: Ten pre-clinical and two clinical studies were eligible for inclusion in this systematic review. We identified the therapeutic roles of medicinal plants Brassica napus, Gardenia jasminoides, Gastrodia elata, Ginkgo biloba, Glycyrrhiza inflata, Paeonia lactiflora, Pueraria lobata and Rehmannia glutinosa; herbal formulations Shaoyao Gancao Tang and Zhengan Xifeng Tang; and medicinal mushroom Hericium erinaceus in the treatment of HA. In this review, we evaluated the mode of actions contributing to their therapeutic effects, including activation of the ubiquitin-proteasome system, activation of antioxidant pathways, maintenance of intracellular calcium homeostasis and regulation of chaperones. We also briefly highlighted the integral cellular signalling pathways responsible for orchestrating the mode of actions.

    CONCLUSION: We reviewed the therapeutic roles of natural remedies in improving or halting the progression of HA, which warrant further study for applications into clinical practice.

    Matched MeSH terms: Brassica napus
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