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Fulltext Fusarium species isolated from mangrove soil in kampung pantai acheh, balik pulau, pulau pinang, malaysia

Latiffah Z, Mah Kok F, Heng Mei H, Maziah Z, Baharuddin S

Trop Life Sci Res, 2010 Aug;21(1):21-9.
PMID: 24575187 MyJurnal

A total of 33 isolates of Fusarium sp. were isolated from soil samples collected from a mangrove forest in an area in Kampung Pantai Acheh, Balik Pulau, Pulau Pinang, Malaysia. The isolates were isolated using soil dilution, direct isolation and debris isolation techniques. The debris isolation technique yielded the most isolates, with a total of 22 Fusarium isolates. Based on identification using morphological characteristics, three Fusarium species were identified: F. solani, F. oxysporum and F. verticillioides. F. solani (91%) was the most common species recovered from the mangrove soil samples, followed by F. oxysporum (6%) and F. verticillioides (3%).

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Evaluation of the effect of radio frequency interference on global positioning system (GPS) receivers via GPS simulation

Dinesh, S., Faudzi, M.M., Rafidah, M., Shakhira, B.N.I., Robiah, A.S., Shalini, S.S., et al.

ASM Science Journal, 2014;8(1):11-20.
MyJurnal

In this study, Global Positioning System (GPS) simulation was employed to study the effect of radio frequency interference (RFI) on two hand-held GPS receivers; Garmin GPSmap 60CSx (evaluated GPS receiver) and Garmin GPSmap 60CS (reference GPS receiver). Both GPS receivers employed the GPS L1 coarse acquisition (C/A) signal. It was observed that the interference signal power levels required to affect the location fixes of the GPS receivers were significantly high compared to the corresponding GPS signal power levels. The noiselike C/A code structure, which modulated the L1 signal over a 2 MHz bandwidth, allowed for the signal to be received at low levels of interferences. The evaluated GPS receiver had better RFI operability as compared to the reference GPS receiver. This is because the evaluated GPS receiver had higher receiver sensitivity, allowing it to have increased carrier-to-noise density (C/N0) levels for GPS satellites tracked by the receiver. The absence of other error parameters, including ionospheric and tropospheric delays, satellite clock, ephemeris and multipath errors, and unintentional signal interferences and obstructions, resulted in the required minimum jamming power levels in this study to be significantly higher as compared to field evaluations. These minimum jamming power levels vary with location and time. This was due to the GPS satellite constellation being dynamic, causing varying GPS satellite geometry over location and time, resulting in the minimum required GPS jamming power levels being location / time dependent. In general, the lowest minimum jamming power levels were observed for readings with the highest position dilution of precision (PDOP) values, and vice versa.

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Diversity of microfungi in sandy beach soil of teluk aling, pulau pinang

Zakaria L, Yee TL, Zakaria M, Salleh B

Trop Life Sci Res, 2011 May;22(1):71-80.
PMID: 24575210 MyJurnal

A total of 82 isolates of microfungi were isolated from 6 sandy soil samples collected from Teluk Aling beach, Pulau Pinang. The soil microfungi were isolated by using direct isolation, debris isolation and soil dilution techniques. Based on morphological characteristics, seven genera of microfungi were identified namely, Fusarium (42%), Aspergillus (24%), Trichoderma (13%), Curvularia (9%), Colletotrichum (6%), Helminthosporium (4%) and Penicillium (2%). The most common species isolated was Fusarium solani followed by Fusarium semitecum, Aspergillus niger, Trichoderma viride, Curvularia clavata, Curvularia lunata, Helminthosporium velutinum, Colletotrichum sp. and Penicillium chrysogenum. From the present study, it appears that the sandy beach contains a microfungi reservoir comprising of a variety of genera which contributes significantly to the ecological functioning of a marine ecosystem.

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Helminthiasis among primary rural school children in Bachok, Kelantan

Anees, A.H., Zulkifli, A., Azmi, A., Syukri, M.

Malaysian Journal of Public Health Medicine, 2003;3(2):19-22.
MyJurnal

A cross-sectional study on helminthiasis among rural primary schoolchildren aged 9 to 10 years Bachok, Kelaritan was perfumed. A total of 680 schoolchildren participated in the study. Stool specimens were examined for the presence of the ova of Ascaris lumbricoides, Trichuris trichiura and hoolcwonn. The worm load was then measured using the modified Stoll`s volumetric dilution
technique. The overall prevalence of helminthiasis was 77 .2%. Trichuris trichiura were the commonest type of heminth noted - 66.8%, compared with Ascaris lumlrricoides (49.7%) and hookworm (1.8%). Mixed infections with Ascaris lumlyricoides and Trichuris trichiura was the commonest type of infection 249(41 .5%) . For Ascaris lumbncoides, 34.6% had mild and 5 I .3% had moderate worm load while for Trichurb trichiura, 66.5% had mild and 30.8 % had moderate worm load. Only 14.1% and 2.7% 4 of the schoolchildren had a heavy load of Ascaris lumlwicoides and Trichuris trichiura respectively. All the schookhildren with hookworm were only mildly infected. Targeted mass treatment for rural Mahysian schoolchildren is still essential, especially in areas where poverty and malnutrition are still prevalent.

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Cosmos caudatus Kunth. extract reduced number of microflora in oyster mushroom (Pleurotus ostreatus)

Yusoff, N. A. H., Sanuan, F. M., Rukayadi, Y.

International Food Research Journal, 2015;22(5):1837-1842.
MyJurnal

Nowadays consumer is more demand on natural foodstuff instead of synthetic product due to their concern on health. The objective of this study is to investigate the effect of C. caudatus extract on the number of microflora in oyster mushroom at different concentration of C. caudatus extract and exposure time using dilution method. The results showed that the number of microorganisms (Log10 CFU/g) in oyster mushroom in term of Total Plate Count (TPC), Bacillus cereus, Escherichia coli and Staphylococcus aureus were 6.13 ± 0.04, 6.15 ± 0.09, 5.97 ± 0.04, and 6.46 ± 0.00, respectively. The effect of C. caudatus extract on microflora in oyster mushroom at concentrations of 0.00%, 0.05%, 0.5%, and 5.0% with exposure time of 0, 5, 10, and 15 min demonstrated that the reduction number of microflora in oyster mushroom was dependent on the concentration of C. caudatus extract and exposure times. The number of TPC (Log10 CFU/g) in oyster mushroom was significantly reduced after treated with C. caudatus extract at concentration of 0.05% for 15 min; 6.13 ± 0.04 reduced to 2.62 ± 0.07. Moreover, B. cereus (Log10 CFU/g) in oyster mushroom was significantly reduced by treatment of C. caudatus extract at concentration of 0.05% for 5 min; 6.15 ± 0.09 reduced to 3.77 ± 0.15. Meanwhile, the number of E. coli (Log10 CFU/g) in oyster mushroom was significantly reduced at concentration of 0.05% for 10 min; 5.97 ± 0.04 reduced to 3.21 ± 0.13. Lastly, the survival number of S. aureus in oyster mushroom was significantly reduced after treated with C. caudatus extract at concentration of 0.05% for 15 min; 6.46 ± 0.00 reduced to 4.83 ± 0.07. In conclusion, C. caudatus extract has potentiality to be developed as natural sanitizer for rinsing raw food materials such as oyster mushroom.

Matched MeSH terms: Indicator Dilution Techniques
Validation of bioelectrical impedance analysis for total body water assessment against the deuterium dilution technique in Asian children

Liu A, Byrne NM, Ma G, Nasreddine L, Trinidad TP, Kijboonchoo K, et al.

Eur J Clin Nutr, 2011 Dec;65(12):1321-7.
PMID: 21731041 DOI: 10.1038/ejcn.2011.122

To develop and cross-validate bioelectrical impedance analysis (BIA) prediction equations of total body water (TBW) and fat-free mass (FFM) for Asian pre-pubertal children from China, Lebanon, Malaysia, Philippines and Thailand.

Matched MeSH terms: Indicator Dilution Techniques*
Fulltext 4-(2-(1H-Benzo[d]imidazol-2-ylthio)acetamido)-N-(substituted phenyl)benzamides: design, synthesis and biological evaluation

Tahlan S, Ramasamy K, Lim SM, Shah SAA, Mani V, Narasimhan B

BMC Chem, 2019 Dec;13(1):12.
PMID: 31384761 DOI: 10.1186/s13065-019-0533-7

Background: Dihydrofolate reductase (DHFR) is an important target for antimetabolite class of antimicrobials because it participates in purine synthesis. 2-mercaptobenzimidazole (2MBI) has similar structural features as purine nucleotides. Given that benzimidazole and similar heteroaromatics have been broadly examined for their anticancer potential, so, we hereby report the design, synthesis and biological studies (i.e. antimicrobial and anticancer studies) of 2MBI derivatives.
Methodology: The antimicrobial activity of synthesized 2MBI derivatives were evaluated against Gram positive and Gram negative bacterial species as well as fungal species by tube dilution technique whereas their anticancer activity was assessed against human colorectal carcinoma cell line (HCT116) by Sulforhodamine B (SRB) assay. They were also structurally characterized by IR, NMR, MS and elemental analyses.
Results discussion and conclusion: The antimicrobial activity findings revealed that compound N1 (MIC
bs,st,
ca
= 1.27, 2.54, 1.27 µM), N8 (MIC
ec
= 1.43 µM), N22 (MIC
kp,an
= 2.60 µM), N23 and N25 (MIC
sa
= 2.65 µM) exhibited significant antimicrobial effects against tested strains, i.e. Gram-positive, Gram-negative (bacterial) and fungal strains. The anticancer screening results demonstrated that compounds N9, N18 (IC50 = 5.85, 4.53 µM) were the most potent compounds against cancer cell line (HCT116) even more than 5-FU, the standard drug (IC50 = 9.99 µM).

Matched MeSH terms: Indicator Dilution Techniques
Oral Nanoemulsion of Fenofibrate: Formulation, Characterization, and In Vitro Drug Release Studies

Gulati N, Kumar Chellappan D, M Tambuwala M, A A Aljabali A, Prasher P, Kumar Singh S, et al.

Assay Drug Dev Technol, 2021 05 14;19(4):246-261.
PMID: 33989048 DOI: 10.1089/adt.2021.012

Nanoemulsions (NMs) are one of the most important colloidal dispersion systems that are primarily used to improve the solubility of poorly water soluble drugs. The main objectives of this study were, first, to prepare an NM loaded with fenofibrate using a high shear homogenization technique and, second, to study the effect of variable using a central composite design. Twenty batches of fenofibrate-loaded NM formulations were prepared. The formed NMs were subjected to droplet size analysis, zeta potential, entrapment efficiency, pH, dilution, polydispersity index, transmission electron microscopy (TEM), Fourier transform infrared spectrophotometry, differential scanning calorimetry (DSC), and in vitro drug release study. Analysis of variance was used for entrapment efficiency data to study the fitness and significance of the design. The NM-7 batch formulation demonstrated maximum entrapment efficiency (81.82%) with lowest droplet size (72.28 nm), and was thus chosen as the optimized batch. TEM analysis revealed that the NM was well dispersed with droplet sizes <100 nm. Incorporation of the drug into the NM was confirmed with DSC studies. In addition, the batch NM-7 also showed the maximum in vitro drug release (87.6%) in a 0.05 M sodium lauryl sulfate solution. The release data revealed that the NM followed first-order kinetics. The outcomes of the study revealed the development of a stable oral NM containing fenofibrate using the high shear homogenization technique. This approach may aid in further enhancing the oral bioavailability of fenofibrate, which requires further in vivo studies.

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Impact of pre-dilution and flushing on continuous renal replacement therapy

Koh KH

Singapore Med J, 2006 Sep;47(9):785-95.
PMID: 16924361

Infusing the replacement solution before the filter (pre-dilution) and regular flushing have not been accounted for in conventional mathematical equations. Their effects on various continuous renal replacement therapy (CRRT) parameters, such as ultrafiltration fraction and urea clearance, have not been well studied. We incorporated these parameters into mathematical equations to help in understanding and prescribing CRRT.

Matched MeSH terms: Indicator Dilution Techniques
Fulltext Evaluation of antimicrobial effect of azadirachtin plant extract (Soluneem (™)) on commonly found root canal pathogenic microorganisms (viz. Enterococcus faecalis) in primary teeth: A microbiological study

Shah S, Venkataraghavan K, Choudhary P, Mohammad S, Trivedi K, Shah SG

J Indian Soc Pedod Prev Dent, 2016 Jul-Sep;34(3):210-6.
PMID: 27461802 DOI: 10.4103/0970-4388.186741

AIM: The aim of this study is to evaluate the antimicrobial activity of Soluneem ™ when used as an irrigating solution along with other commonly used irrigating solution sodium hypochlorite (NaOCl) against Enterococcus faecalis.

MATERIALS AND METHODS: Microorganism used in this study was E. faecalis (Microbial Type Culture Collection 439). Test substance used was Soluneem ™, which was obtained from Vittal Mallya Scientific Research Foundation (VMSRF), Bengaluru. This study was conducted in a microbiology laboratory (Biocare Research India Pvt., Ltd. Laboratory, Ahmedabad, Gujarat) to evaluate the antimicrobial effect of Soluneem ™ (Azadirachtin) on E. faecalis. Antimicrobial activity testing was performed using the macrobroth dilution method according to the Clinical Laboratory Standards Institute guidelines. All determinations were performed thrice.

RESULTS: Minimum bactericidal concentration (MBC) was seen as 2.6% for Soluneem ™ while the same was seen at 0.1% for NaOCl. Independent sample t-test was carried out to compare the MBC of Soluneem ™ and NaOCl, which showed that there was no statistically significant difference between them, i.e., 2.6% Soluneem ™ was as effective as 0.1% NaOCl.

CONCLUSION: Soluneem ™ showed antimicrobial activity against E. faecalis at various concentrations. It was also found that the efficacy of Soluneem ™ at 2.6% concentration and above was relatively similar to that of gold standard irrigating solution (NaOCl) on inhibition of E. faecalis.

Matched MeSH terms: Indicator Dilution Techniques