MyMedR

Fulltext Neisseria meningitidis serotype W135

Wahab ZA, Yahaya H

Med J Malaysia, 2000 Sep;55(3):388.

PMID: 11200725

Matched MeSH terms: Neisseria meningitidis/immunology*

Fulltext Invasive meningococcal disease in Malaysia, Philippines, Thailand, and Vietnam: An Asia-Pacific expert group perspective on current epidemiology and vaccination policies

Thisyakorn U, Carlos J, Chotpitayasunondh T, Dien TM, Gonzales MLAM, Huong NTL, et al.

Hum Vaccin Immunother, 2022 Nov 30;18(6):2110759.

PMID: 36084311 DOI: 10.1080/21645515.2022.2110759

Invasive meningococcal disease (IMD) imposes a significant burden on the global community due to its high case fatality rate (4-20%) and the risk of long-term sequelae for one in five survivors. An expert group meeting was held to discuss the epidemiology of IMD and immunization policies in Malaysia, Philippines, Thailand, and Vietnam. Most of these countries do not include meningococcal immunization in their routine vaccination programs, except for high-risk groups such as immunocompromised people and pilgrims. It is difficult to estimate the epidemiology of IMD in the highly diverse Asia-Pacific region, but available evidence indicate serogroup B is increasingly dominant. Disease surveillance systems differ by country. IMD is not a notifiable disease in some of them. Without an adequate surveillance system in the region, the risk and the burden of IMD might well be underestimated. With the availability of new combined meningococcal vaccines and the World Health Organization roadmap to defeat bacterial meningitis by 2030, a better understanding of the epidemiology of IMD in the Asia-Pacific region is needed.

Matched MeSH terms: Neisseria meningitidis*

Fulltext Neisseria meningitidis isolates with moderate susceptibility to penicillin

Ariza A, Rohani MY

Med J Malaysia, 2004 Oct;59(4):558-9.

PMID: 15779597 MyJurnal

Neisseria meningitidis is one of the important cause of meningitis and has been extremely susceptible to penicillin. Nevertheless, moderately penicillin resistant strains have been reported in some parts of the world. To our knowledge, there is no such report in Malaysia. We report two clinical isolates that were found to have MICs of decreased susceptibility to penicillin by the agar dilution method.

Matched MeSH terms: Neisseria meningitidis/drug effects*; Neisseria meningitidis/isolation & purification

Fulltext Plasmids in penicillinase-producing Neisseria gonorrhoeae in Peninsular Malaysia

Koh CL, Kalaimathee KK, Ngeow YF

Med J Malaysia, 1984 Dec;39(4):269-71.

PMID: 6443581

The plasmid profiles of 66 strains of penicillinase-producing Neisseria gonorrhoeae (PPNG), isolated in Peninsular Malaysia, were determined by agarose gel electrophoresis. All the isolates harboured two common plasmid species, a 4.4 x 10 6 Far-East type R plasmid associated with beta-lactamase production and a 2.6 x 106 plasmid of unknown function. In addition to these two plasmids, 51 (77%) PPNG isolates also carried a 24.5 x 106 conjugative plasmid.

Matched MeSH terms: Neisseria gonorrhoeae/metabolism*

Fulltext Meningococcal disease surveillance in the Asia-Pacific region (2020): The global meningococcal initiative

Aye AMM, Bai X, Borrow R, Bory S, Carlos J, Caugant DA, et al.

J Infect, 2020 11;81(5):698-711.

PMID: 32730999 DOI: 10.1016/j.jinf.2020.07.025

The degree of surveillance data and control strategies for invasive meningococcal disease (IMD) varies across the Asia-Pacific region. IMD cases are often reported throughout the region, but the disease is not notifiable in some countries, including Myanmar, Bangladesh and Malaysia. Although there remains a paucity of data from many countries, specific nations have introduced additional surveillance measures. The incidence of IMD is low and similar across the represented countries (<0.2 cases per 100,000 persons per year), with the predominant serogroups of Neisseria meningitidis being B, W and Y, although serogroups A and X are present in some areas. Resistance to ciprofloxacin is also of concern, with the close monitoring of antibiotic-resistant clonal complexes (e.g., cc4821) being a priority. Meningococcal vaccination is only included in a few National Immunization Programs, but is recommended for high-risk groups, including travellers (such as pilgrims) and people with complement deficiencies or human immunodeficiency virus (HIV). Both polysaccharide and conjugate vaccines form part of recommendations. However, cost and misconceptions remain limiting factors in vaccine uptake, despite conjugate vaccines preventing the acquisition of carriage.

Matched MeSH terms: Neisseria meningitidis*

Serological ecology of Neisseria gonorrhoeae (PPNG and non-PPNG) strains: Canadian perspective

Dillon JR, Bygdeman SM, Sandström EG

Genitourin Med, 1987 Jun;63(3):160-8.

PMID: 3111978

One hundred and thirty eight penicillinase producing Neisseria gonorrhoeae (PPNG) and 239 non-PPNG strains were characterised serologically using a panel of seven monoclonal antibodies directed against protein 1A and seven against protein 1B. An association between serovar and susceptibility to antimicrobial agents, auxotype, and plasmid content was observed. Serogroup WI strains were more sensitive to penicillin, ampicillin, tetracycline, erythromycin, cefoxitin, and cefuroxime. Sixty five (82%) of the 79 WI strains were typed as being serovar Aedgkih, and 47 (72%) of these strains required arginine, uracil, and hypoxanthine for growth (AUH-). Seventy one (44%) of 160 WII/WIII strains were serovar Bacejk, and 42 (59%) of these required proline, citrulline, and uracil for growth (PCU-) and were plasmid free. Serovars Bcgk, Beghjk, Bacjk, and Bajk were associated with resistance to antimicrobial agents. Analysis of PPNG isolates showed a new serovar, Af, which was associated with strains imported from Malaysia and Singapore that required proline and ornithine for growth (Pro-Orn-) and carried the 24.5 megadalton transfer plasmid, the 2.6 megadalton cryptic plasmid, and the 4.5 megadalton penicillinase producing plasmid. Other associations between serovar and geographical location were noted.

Matched MeSH terms: Neisseria gonorrhoeae/classification*; Neisseria gonorrhoeae/drug effects; Neisseria gonorrhoeae/enzymology

Plasmid profiles of Neisseria gonorrhoeae in Peninsular Malaysia

Kalaimathee KK, Koh CL, Ngeow YF

Microbiol. Immunol., 1985;29(10):921-6.

PMID: 3935906

The plasmid profiles of 160 strains of Neisseria gonorrhoeae isolated in Peninsular Malaysia, comprising 80 penicillinase-producing (PPNG) and 80 non-penicillinase-producing (non-PPNG) isolates, were determined. The 80 PPNG isolates were divided into two plasmid groups. All of them harbored two common plasmid species, a 4.4 megadalton (Md) R plasmid previously associated with beta-lactamase production in PPNG strains from the Far East and a 2.6 Md multicopy plasmid of unknown function. In addition to these two plasmids, 60 (75%) PPNG isolates also carried a large 24.5 Md conjugative plasmid. In contrast, the 80 non-PPNG strains were divided into three plasmid groups. All of them possessed the 2.6 Md cryptic plasmid, and 35 (44%) isolates also harbored the 24.5 Md transfer plasmid. Besides these two plasmids, one non-PPNG isolate carried an additional 7.8 Md cryptic plasmid.

Matched MeSH terms: Neisseria gonorrhoeae/enzymology; Neisseria gonorrhoeae/genetics*; Neisseria gonorrhoeae/isolation & purification

Auxotypes and serogroups of tetracycline-resistant Neisseria gonorrhoeae isolated in Malaysia

Koay AS, My R, Cheong YM

J Clin Microbiol, 1996 Jul;34(7):1863-5.

PMID: 8784614

Between 1992 and 1994, 253 tetracycline-resistant Neisseria gonorrhoeae (TRNG) strains were isolated and characterized by auxotype and serogroup (A/S) classes to study TRNG prevalence in different years. TRNG accounted for 28.1, 42.5, and 51.9% of the strains isolated in 1992, 1993, and 1994, respectively, showing a significant increase in each successive year (chi square = 26.7, P < 0.001). There was no significant increase in penicillinase-producing TRNG, which accounted for 53.1, 53.8, and 63.2% of the TRNG isolates. The 253 TRNG isolates belonged to 53 A/S classes. Eighteen A/S classes not observed in 1992 were detected in 1993, and 11 A/S classes not observed in 1992 and 1993 were isolated in 1994, indicating dissemination of the tetracycline resistance gene among the N. gonorrhoeae strains in Malaysia. Its emergence and subsequent rapid spread are alarming. The plasmid is capable of self-transfer (S.A. Morse, S.R. Johnson, J.W. Biddle, and M.C. Roberts, J. Infect. Dis. 155:819-822, 1987), allowing further dissemination of tetracycline resistance.

Matched MeSH terms: Neisseria gonorrhoeae/classification*; Neisseria gonorrhoeae/drug effects*; Neisseria gonorrhoeae/genetics

The laboratory diagnosis of veneral diseases--II--the laboratory diagnosis of gonorrhoea

Jegathesan M

Med J Malaysia, 1974 Sep;29(1):66-9.

PMID: 4282635

Matched MeSH terms: Neisseria gonorrhoeae/growth & development

Fulltext Serogroups and antibiotic susceptibility patterns of Neisseria meningitidis isolated from army recruits in a training camp

Rohani MY, Ahmad Afkhar F, Amir MA, Muhd Amir K, Sahura H, Fairuz A, et al.

Malays J Pathol, 2007 Dec;29(2):91-4.

PMID: 19108400 MyJurnal

Invasive Neisseria meningitidis infection is rare but carries a high mortality rate. The carriage rate in the normal population is around 10% and can be higher in confined populations. A study on the prevalence of carriage of N. meningitidis was conducted among 3195 army recruits after 2 months of intensive training in an army camp. N. meningitidis was isolated from 37.0% of these recruits. Two hundred and ten of N. meningitidis isolates were subjected to serogrouping and 100 to antibiotic sensitivity testing by the disc diffusion method and E-test for penicillin. Ten (4.8%) of 210 Neisseria meningitidis serogrouped belonged to serogroup W135, 3.33% serogroup A and 81.4% belonged to either serogroup X, Y or Z. With the agar disc diffusion method, all the N. meningitidis showed susceptiblity to chloramphenicol, rifampicin, cefotaxime and levofloxacin; 85% of the strains were resistant to cotrimoxazole and 12.5% resistant to penicillin. However, based on minimum inhibitory concentration, none of the Neisseria meningitidis tested was resistant to penicillin.

Matched MeSH terms: Neisseria meningitidis/drug effects*; Neisseria meningitidis/genetics*

Mutations in gyrA and parC in ciprofloxacin-resistant Neisseria gonorrhoeae in Kuala Lumpur, Malaysia

Karunakaran R, Sam IC

J Antimicrob Chemother, 2007 Apr;59(4):803-4.

PMID: 17329273

Matched MeSH terms: Neisseria gonorrhoeae/drug effects*; Neisseria gonorrhoeae/genetics*

Infections due to beta-lactamase-producing Neisseria gonorrhoeae at the University Hospital Medical Center, Kuala Lumpur, Malaysia

Ismail R

Sex Transm Dis, 1987 4 1;14(2):113-5.

PMID: 3112967

The incidence of infections due to beta-lactamase-producing Neisseria gonorrhoeae is increasing in many parts of the world. An epidemiologic survey of infections caused by beta-lactamase-producing strains of N. gonorrhoeae at the University Hospital, Kuala Lumpur, from February 1977 to December 1985 (106 months) showed that the incidence rose from 4.8% (two cases) in 1977 to 49.4% (39 cases) by the end of 1985. The highest incidence of gonococcal infections was found to be in the group aged 20-39 years; the male-to-female ratio was 1.55:1. The mean inhibitory concentrations of benzylpenicillin were 0.12 microgram/ml for non-beta-lactamase-producing strains and 16 micrograms/ml for isolates of N. gonorrhoeae that produce beta-lactamase.

Matched MeSH terms: Neisseria gonorrhoeae/enzymology*; Neisseria gonorrhoeae/isolation & purification

mazE Antitoxin of Toxin Antitoxin System and fbpA as Reliable Targets to Eradication of Neisseria meningitidis

Maleki A, Ghafourian S, Pakzad I, Badakhsh B, Sadeghifard N

Curr Pharm Des, 2018;24(11):1204-1210.

PMID: 29237374 DOI: 10.2174/1381612824666171213094730

BACKGROUND: Neisseria meningitidis is considered as a dangerous pathogen threatening human health. Nowadays, the new drug target is focused. Toxin antitoxin (TA) system is recently identified as an antimicrobial drug target. Also, in N. meningitidis, iron-uptake system could be an interesting target for drug discovery.

METHODS: In this study, fbpA and mazE genes were chosen as new antimicrobial targets and treated with antisense peptide nucleic acid (PNA). Firstly, they were evaluated by bioinformatics and then analyzed by experimental procedures. Secondly, the functionality was evaluated by stress conditions.

RESULTS: Our results interestingly demonstrated that when fbpA and mazE loci of N. meningitidis were targeted by antisense PNA, 8 µM concentration of fbpA-PNA as well as 30 µM concentration of mazE-PNA inhibited the growth of N. meningitides and were found to be bacteriostatic, whereas 10 μM concentration of fbpA-PNA showed bacteriocidal activity.

CONCLUSION: Our findings demonstrated the bactriocidal activity of fbpA-PNA and bacteriostatic activity of mazEPNA. Therefore, mazE and fbpA genes should be potent antimicrobial targets but further analysis including in vivo analysis should be performed.

Matched MeSH terms: Neisseria meningitidis/drug effects*; Neisseria meningitidis/genetics

Phenotypic and Genotypic Characterization of Neisseria gonorrhoeae Isolates from New Zealand with Reduced Susceptibility to Ceftriaxone

Jamaludin N, Gedye K, Collins-Emerson J, Benschop J, Nulsen M

Microb Drug Resist, 2019 Sep;25(7):1003-1011.

PMID: 31021281 DOI: 10.1089/mdr.2018.0111

Aim:
To characterize mutations in penA, mtrR, ponA, and porBIB, considered target genes for antimicrobial resistance, in Neisseria gonorrhoeae isolates with elevated minimum inhibitory concentrations (MICs) of ceftriaxone cultured from patients in New Zealand.
Results:
Out of 28 isolates supplied by the Institute of Environmental Science and Research Limited (ESR), Porirua, New Zealand, 14 were found to show reduced susceptibility to ceftriaxone (MIC of 0.06 mg/L) according to criteria used by the ESR and the Australian Gonococcal Surveillance Programme (AGSP) when tested in our laboratory. Rates of resistance to ciprofloxacin, azithromycin, penicillin, and tetracycline were 100% (28/28), 7% (2/28), 36% (10/28), and 25% (7/28), respectively. Ten different penA (Penicillin binding protein 2 [PBP2]) sequences were observed. The most common mosaic penA M-1 resembled mosaic penA XXXIV, which has been associated with ceftriaxone treatment failures in other countries. Four semimosaic PBP2 sequences were observed and may be novel PBP sequences, while four out of five nonmosaic PBP2 sequences were similar to PBP2 sequences reported in Australia. Twenty-one isolates harbored mutations in all 4 genes (penA, mtrR, porBIB, and ponA), and 13 of these exhibited reduced susceptibility to ceftriaxone.
Conclusion:
Mutations in penA, mtrR, porBIB, and ponA observed in this study may have contributed to reduced susceptibility to ceftriaxone among New Zealand gonococcal isolates. Over half (16/22) of mosaic penA sequences from the gonococcal isolates resembled penA XXXIV.

Matched MeSH terms: Neisseria gonorrhoeae/drug effects*; Neisseria gonorrhoeae/genetics*; Neisseria gonorrhoeae/isolation & purification

Fulltext Disseminated Multidrug Resistant Neisseria gonorrhoea infection in a patient with vasculitic skin rash

Lee KS, Zaid M, Ong ELC

Rev Soc Bras Med Trop, 2023;56:e02892023.

PMID: 37792836 DOI: 10.1590/0037-8682-0289-2023

Matched MeSH terms: Neisseria gonorrhoeae

Fulltext Excellent outcome of primary Neisseria meningitidis keratoconjunctivitis

Daud J, Ishak SR, Deris ZZ, Hitam WH

Asian Pac J Trop Biomed, 2011 Oct;1(5):419-20.

PMID: 23569805 DOI: 10.1016/S2221-1691(11)60092-0

Infectious conjunctivitis is a very common presentation to medical professional and ophthalmologist all over the world. Although its typically self-limiting and treatable in almost all of the cases, but we need to be aware of the rare and potentially life threatening if the cause is not promptly identified and treated accordingly. In our case report, we highlighted the rare case of Neisseria meningitidis as a primary cause of keratoconjunctivitis. Neisseria meningitidis is a rare etiology of keratoconjunctivitis and its ocular presentations are quite similar with other bacterial or viral infection. The infection may potentially fatal if systemic invasion occurred, however with immediate and proper treatment the outcome is satisfactory. Early diagnosis and proper antibiotic treatment are critical to prevent systemic spread of the infection. Public health intervention is needed to prevent outbreak of the disease.

Matched MeSH terms: Neisseria meningitidis*

Fulltext Identification of Neisseria gonorrhoeae as the causative agent in a case of culture-negative dermatitis-arthritis syndrome using real-time PCR

Kimmitt PT, Kirby A, Perera N, Nicholson KG, Schober PC, Rajakumar K, et al.

J Travel Med, 2008;15(5):369-71.

PMID: 19006515 DOI: 10.1111/j.1708-8305.2008.00240.x

Sexually transmitted infections (STIs) are an increasingly common and important cause of a fever in a returning traveler. Systemic complications of STIs, human immunodeficiency virus seroconversion illness, and secondary syphilis are diagnoses that can easily be missed. We present a case of culture-negative disseminated gonococcal infection presenting with fever, malaise, polyarthralgia, arthritis, and a rash that developed following orogenital contact and was diagnosed using real-time polymerase chain reaction. This technology has major potential to improve the speed and sensitivity of diagnosis and consequent management of patients with this syndrome.
Study site: United Kingdom (patient had recent travel to Thailand and Malaysia)

Matched MeSH terms: Neisseria gonorrhoeae/isolation & purification

Rectal microscopy in men - expensive, time consuming and inappropriate

Waters L, Worthen E, O'mahony C

Int J STD AIDS, 2006 Oct;17(10):710.

PMID: 17059644

Matched MeSH terms: Neisseria gonorrhoeae/drug effects*

Multi-resistant Neisseria gonorrhoeae in Kuala Lumpur, Malaysia

Sam IC, Ngeow YF

Int J STD AIDS, 2006 Oct;17(10):710-1.

PMID: 17059643

Matched MeSH terms: Neisseria gonorrhoeae/isolation & purification*

Invasive meningococcal disease in the University of Malaya Medical Centre, Kuala Lumpur, Malaysia