Browse publications by year: 2009

  1. Lim V, Stubbs JW, Nahar N, Amarasena N, Chaudry ZU, Weng SCK, et al.
    Lancet, 2009 Sep 19;374(9694):973.
    PMID: 19762076 DOI: 10.1016/S0140-6736(09)61641-X
    MeSH terms: Forecasting; Humans; Leadership; Physician's Role*; Politics*; Global Health*; Greenhouse Effect*
  2. Rosli Y, Maddess T, Dawel A, James AC
    Clin Neurophysiol, 2009 Dec;120(12):2100-2108.
    PMID: 19846337 DOI: 10.1016/j.clinph.2009.09.006
    OBJECTIVE: To examine the feasibility of a multifocal visual evoked potential (mfVEP) binocularly, using a variant of the multifocal frequency-doubling (FD) pattern-electroretinogram (MFP).

    METHODS: Stimuli were presented in both monocular and dichoptic conditions at eight visual field locations/eye. The incommensurate stimulus frequencies ranged from 15.45 to 21.51 Hz. Five stimulus conditions differing in spatial frequency and orientation were examined for three viewing conditions. The resulting 15 stimulus conditions were examined in 16 normal subjects who repeated all conditions twice.

    RESULTS: Several significant independent effects were identified. Response amplitudes were reduced for dichoptic viewing (by 0.85 times, p<4 x 10(-11)); offset by increases in responses for between eye differences of one octave of spatial frequency: lower (1.15 times, 0.1 cpd); higher (1.29 times, 0.4 cpd), both p<1.8 x 10(-7). Crossed orientations produced significant effects upon response phase (p=0.023) but not amplitude (p=0.062).

    CONCLUSIONS: The results indicated that dichoptic evoked potentials using multifocal frequency-doubling illusion stimuli are practical. The use of crossed orientation, or differing spatial frequencies, in the two eyes reduced binocular interactions.

    SIGNIFICANCE: The results indicate a method wherein several spatial or temporal and frequencies per visual field region can be tested in reasonable time using a multifocal VEP using spatial frequency-doubling stimuli.

    MeSH terms: Adult; Evoked Potentials, Visual/physiology*; Female; Humans; Male; Middle Aged; Pattern Recognition, Visual/physiology*; Photic Stimulation/methods*; Vision, Binocular/physiology; Young Adult
  3. Chew FLM, Subrayan V, Chong PP, Goh MC, Ng KP
    Jpn. J. Ophthalmol., 2009 Nov;53(6):657-659.
    PMID: 20020251 DOI: 10.1007/s10384-009-0722-3
    MeSH terms: Accidents, Traffic; Adult; Antifungal Agents/therapeutic use; Base Sequence; Cladosporium/classification; Cladosporium/genetics; Cladosporium/isolation & purification*; Corneal Diseases/diagnosis; Corneal Diseases/drug therapy; Corneal Diseases/microbiology*; DNA, Fungal/analysis; Eye Injuries/diagnosis; Eye Injuries/drug therapy; Eye Injuries/microbiology*; Humans; Male; Molecular Sequence Data; Mycoses/diagnosis; Mycoses/drug therapy; Mycoses/microbiology*; Natamycin/therapeutic use; Wound Infection/diagnosis; Wound Infection/drug therapy; Wound Infection/microbiology*; Eye Infections, Fungal/diagnosis; Eye Infections, Fungal/drug therapy; Eye Infections, Fungal/microbiology*; Polymerase Chain Reaction; Mycological Typing Techniques; Lacerations/diagnosis; Lacerations/drug therapy; Lacerations/microbiology; Corneal Injuries*
  4. Viprakasit V, Lee-Lee C, Chong QT, Lin KH, Khuhapinant A
    Int. J. Hematol., 2009 Nov;90(4):435-445.
    PMID: 19862602 DOI: 10.1007/s12185-009-0432-0
    Worldwide, thalassemia is the most commonly inherited hemolytic anemia, and it is most prevalent in Asia and the Middle East. Iron overload represents a significant problem in patients with transfusion-dependent beta-thalassemia. Chelation therapy with deferoxamine has traditionally been the standard therapeutic option but its usage is tempered by suboptimal patient compliance due to the discomfort and demands associated with the administration regimen. Therefore, a great deal of attention has been focused on the development of oral chelating agents. Deferiprone, even though available for nearly two decades in Asia with recent encouraging data on cardiac iron removal and long-term efficacy, has serious adverse effects including agranulocytosis and neutropenia which has impeded it from routine clinical practice. A novel oral chelator; deferasirox is effective throughout a 24 h dosing period and both preclinical and clinical data indicate that it successfully removes both hepatic and cardiac iron. In Asia, optimal management of severe thalassemia patients and the availability and access to oral iron chelators still presents a major challenge in many countries. In this regard, the development and implementation of consensus guidelines for management of Asian patients with transfusion-dependent thalassemia will be a major step towards improving and maintaining the continuity of patient care.
    MeSH terms: Asia/epidemiology; Cross-Cultural Comparison; Drug Administration Routes; Humans; Iron Chelating Agents/administration & dosage; Iron Chelating Agents/pharmacology; Iron Chelating Agents/therapeutic use*; Thalassemia/economics; Thalassemia/epidemiology; Thalassemia/therapy*; Chelation Therapy*; Prevalence; Health Care Costs; beta-Thalassemia/economics; beta-Thalassemia/epidemiology; beta-Thalassemia/therapy; Practice Guidelines as Topic; Iron Overload/drug therapy*; Transfusion Reaction
  5. Krishnan S, Rohen JW, Lütjen-Drecoll E, Carmichael SW, Nicholson HD, Trotman P, et al.
    Surg Radiol Anat, 2009 Sep;31 Suppl 1:43-8.
    PMID: 27392490 DOI: 10.1007/BF03371484
  6. Pereda J, Niimi G, Kaul JM, Mishra S, Pangtey B, Peri D, et al.
    Surg Radiol Anat, 2009 Sep;31 Suppl 1:49-93.
    PMID: 27392491 DOI: 10.1007/BF03371485
  7. Aksu F, Topacoglu H, Arman C, Atac A, Tetik S, Hasanovic A, et al.
    Surg Radiol Anat, 2009 Sep;31 Suppl 1:95-229.
    PMID: 27392492 DOI: 10.1007/BF03371486
    Conference abstracts: Malaysia in affiliation
    IN THE GROWING BODY. Marina Yurievna Kapitonova, Syed Baharom Syed Ahmad Fuad, Flossie Jayakaran; Faculty of Medicine, Universiti Teknologi MARA, Shah Alam, Malaysia
    MASTOID NOTCH OF THE SKULL. ISrijit Das, 2Normadiah Kassim, lAzian Latiff, IFarihah Suhaimi, INorzana Ghafar, lKhin Pa Pa Hlaing, lIsraa Maatoq, IFaizah Othman; I Department of Anatomy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia; 2 Department of Anatomy, Universiti Malaya, Kuala Lumpur, Malaysia. das_sri
    CLINICAL IMPLICATIONS. Srijit Das, Azian Latiff, Parihah Suhaimi, Norzana Ghafar, Khin Pa Pa Hlaing, Israa Maatoq, Paizah Othman; Department of Anatomy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
    MESENCHYMAL CELLS. lSeyed Noureddin Nematollahi-Mahani, lMastafa Latifpour, 2Masood Deilami, 3Behzad Soroure-Azimzadeh, lSeyed
    Hasan Eftekharvaghefi, 4Fatemeh Nabipour, 5Hamid
    Najafipour, 6Nouzar Nakhaee, 7Mohammad Yaghoobi, 8Rana Eftekharvaghefi, 9Parvin Salehinejad, IOHasan Azizi; 1 Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran; 2 Department of Cardiosurgery, Hazrat-e Zahra Hospital, Kerman, Iran; 3 Department of Cardiology, Kerman University of Medical Sciences, Kerman, Iran; 4 Department of Pathology, Kerman University of Medical Sciences, Kerman, Iran; 5 Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran; 6 Department of Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran; 7 Department
    of Biotechnology, Research Institute of Environmental Science, International Center for Science, High Technology & Environmental Science, Kerman, Iran; 8 Students Research Center, Kerman University of Medical Sciences, Kerman, Iran; 9 Institute of Bioscience, University Putra Malaysia,
    Kuala Lumpur, Malaysia; 10 Department of Stem Cell, Cell Science Research Center, Royan Institute, ACECR, Tehran, Iran.
  8. Chai, L.C., Fatimah, C.A., Norhisyam, M.S., Rozila, A., Nadzirah, A.S., Natasha, L.H.Y.
    The objective of the present study was to develop a rapid, reliable and yet inexpensive protocol for genomic DNA extraction from frozen and ethanol-preserved Asian green-lipped mussels for random amplified microsatelite (RAM) analysis. The procedure comprised of three major steps: (1) Tissue degradation by boiling in 6% Chelex 100 resin in TE buffer; (2) Protein digestion by Proteinase K; and (3) DNA precipitation by adding 2 volumes of cold absolute ethanol. The entire procedure can be completed within two hours. The resulting RAM profiles were clear and reproducible. Our results demonstrate that the combined protocol of Chelex 100-Proteinase K-ethanol precipitation is a powerful yet economical DNA isolation method for population genetic studies involving a large sample size.
    MeSH terms: Ethanol; DNA; Genetics, Population; Resins, Plant; Resins, Synthetic; Sample Size; Endopeptidase K; Genomics; Bivalvia; Proteolysis
  9. Irwandi, J., Farida, O.
    A study was conducted to quantitate the concentrations of heavy metals, such as Hg, Pb and Cd in eight species of marine fin fish caught off the coast of Langkawi Island in Malaysia, as well as in its waters. The same fish were also used to determine the content of nutritional minerals, such as copper (Cu), zinc (Zn), calcium (Ca), and manganese (Mn).Fish and water samples were collected from four different areas, namely (1) Main Jetty Pulau Tuba (MJPT), (2) Teluk Cempedak Jetty (TCJ), (3) Simpang Tiga Chian Lian (STCL) and (4) Main Jetty Kuah (MJK) around Langkawi Island. Results showed that for the vital elements, all species had higher concentration of Zn compared to other elements. For the toxic elements, lead (Pb) and mercury (Hg) were found to have lower concentration of the mean values than the permissible limits set by FAO/WHO (1984). However, cadmium (Cd) level was slightly higher than the permissible limit but was still acceptable according to the Malaysian Food Regulation (1985). It can be concluded that all fish species studied are safe to be consumed.
    MeSH terms: Animals; Cadmium; Calcium, Dietary; Copper; Fishes; Malaysia; Manganese; Mercury; Minerals; Water; Zinc; Metals, Heavy; Islands
  10. Sujeewa, A.K.W., Norrakiah, A.S., Laina, M.
    Vibrio parahaemolyticus is prevalent in tropical marine environment in all seasons and can cause seafood-borne gastroenteritis. A total of 251 suspected isolates were tested including 60 from frozen shrimp, 50 from cultured live shrimp, 67 from sediments of culture ponds and 74 from water were subjected to polymerase chain reaction (PCR) targeting the toxR gene for confirmation as V. parahaemolyticus. Of the 128 toxR positive isolates, 15% of the isolates from culture environment (from live shrimp, sediments and water) and 7% of frozen shrimp samples were positive for the tdh and trh genes. Since urease production could be a marker of trh but not tdh in V. parahaemolyticus, a total of 189 of the 251 suspected V. parahaemolyticus isolates were tested for urease production and 41% of the isolates were found to be positive for urease production. However not all urease positive strains of V. parahaemolyticus were positive for either tdh or trh genes. Detection of virulent strains in shrimp culture environment in Malaysia suggests a probable risk for health of people consuming raw shrimp.
    MeSH terms: Gastroenteritis; Malaysia; Seasons; Shellfish; Urease; Vibrio parahaemolyticus; Water; Prevalence; Polymerase Chain Reaction; Seafood; Ponds
  11. Abbas F.M.A., Saifullah, R., Azhar, M.E.
    Cavendish (Musa paradisiaca L, cv cavendshii) and Dream (Musa acuminata colla. AAA, cv ‘Berangan’) banana flours were prepared from ripe fruits collected from eleven markets located in Penang, Malaysia. The mineral composition (Na, K, Ca, Mg, Cu, Fe, Mn, Zn) of the flour were analyzed by atomic absorption spectrophotometer and the data obtained were analyzed using logistic regression model. Ripe banana flours were rich source of K and a fair source of other minerals, however logistic regression model identified Mg as an indicator to discriminate between the two types of banana flour affording 100 % correct assignation. Based on this result, mineral analysis may be suggested as a method to authenticate ripe banana flour. This study also presents the usefulness of logistic regression technique for analysis and interpretation of complex data.
    MeSH terms: Flour; Fruit; Malaysia; Minerals; Regression Analysis; Sodium, Dietary; Spectrophotometry, Atomic; Zinc; Logistic Models; Musa
  12. Ting, A.S.Y, DeCosta, J.L.
    Different probiotic strains are incorporated into cultured-milk drinks by respective manufacturers with the common aim of providing health benefits to the consumers. Four common cultured-milk products (brands N, S, V and Y) were evaluated for their quality of probiotic strains used, based on the susceptibility of the probiotics to various pH levels simulated to mimic the gastrointestinal system. Results showed that brands Y and V have higher initial probiotic inoculum compared to brands N and S although probiotics from brands N and S were more tolerant to pH 3. Generally, all probiotic strains preferred higher pH with highest viability of Lactobacillus spp., Bifidobacterium spp., and Streptococcus thermophilus observed at pH 8.1. Our study also discovered that among the four brands tested, brand V contained probiotic strains which are most likely to remain viable after passage through the gastrointestinal system.
    MeSH terms: Animals; Bifidobacterium; Disease Susceptibility; Lactobacillus; Probiotics; Gastrointestinal Tract; Cultured Milk Products; Streptococcus thermophilus
  13. Zulkifli, Y., Alitheen, N.B., Raha, A.R., Yeap, S.K., Marlina, Son, R., et al.
    Vibrio parahaemolyticus is one of the most widely recognized pathogenic Vibrio species due to numerous outbreaks and its’ wide occurrence in marine environment. In this study, 32 isolates of V. parahaemolyticus isolated from cockles were tested for sensitivity to 16 antibiotics and the presence of plasmids. All the isolates were multi-resistance, defined as resistant to atleast three different antibiotics with multiple antibiotic resistance indexes ranging from 0.31 to 0.69, indicating the isolates originate from high risk sources of contamination where antibiotics are often used. In the plasmid profiling test, only 15 isolates (47%) harbored plasmid DNA, which ranged in size from 2.7 to 56.2 kb, separating the isolates into 14 plasmid profiles. Hence, food contaminated with antibiotic resistant V. parahaemolyticus could be a major threat to public health due to the distinct possibility that they can be a significant reservoir of genes encoding antibiotic resistance determinants that can be transferred intra or interspecies. As in many developing countries, raw food hygiene and antimicrobial resistance epidemiology is still in the infancy stage in the locality of the study and thus our data provide a current baseline profile of antimicrobial resistance and plasmid of V. parahaemolyticusfrom cockles in Padang, Indonesia.
    MeSH terms: Animals; Anti-Infective Agents; Anti-Bacterial Agents; Developing Countries; Disease Outbreaks; DNA; Drug Resistance, Microbial; Hygiene; Indonesia; Plasmids; Public Health; Shellfish; Vibrio parahaemolyticus; Cardiidae; Raw Foods
  14. Azizah, A.H., Wee, K.C., Azizah, O., Azizah, M.
    Effect of various cooking methods on antioxidant content and radical scavenging activity of pumpkin was evaluated. Pumpkin (Cucurbita moschata) was boiled and stir-fried for 2, 4 and 6 minutes respectively. Beta-carotene and lycopene were determined using HPLC and total phenolics measured using Folin-Ciocalteu method. The free radical scavenging activity of the samples was determined using 1, 1-diphenyl-2 picrylhydrazyl assay. Interestingly, result of the study showed an increase in both beta-carotene (2 to 4 times) and lycopene (17 to 40 times) content of pumpkin after cooking for 2, 4 and 6 minutes. However, the treatment resulted in 18 to 54% losses of total phenolics content of the pumpkin. Nevertheless, the free radical scavenging activity exhibited by cooked pumpkins was found to be high, in the range of 81.1% to 94.6% with IC50 of 1.41 to 1.62 mg ml-1
    MeSH terms: Antioxidants; Biphenyl Compounds; Carotenoids; Chromatography, High Pressure Liquid; Cooking; Free Radicals; Plant Extracts; beta Carotene; Inhibitory Concentration 50; Cucurbita
  15. Tan, Y.F., Haresh, K.K., Chai, L.C., Son R.
    A study to determine the antibiotic sensitivity pattern and genotyping using RAPD-PCR was performed on 50 C. jejuni isolated from sushi retailed in different supermarkets. With less than half of the isolates susceptible to the antibiotics tested, resistant to two or more antibiotics were observed in most of the isolates. The banding patterns obtained from RAPD-PCR revealed that no predominant clone exists and the bacterial population is rather diverse. Hence, the resistance of the C. jejuni to different classes of antibiotic as well as their diverse genotypes suggests that these C. jejuni isolates were generated from different sources in the contaminated supermarkets where sushi were retailed. Our data showed that C. jejuni can be an important reservoir for resistance genes and that study with comprehensive collections of samples are urgently required to establish better measures to reduce or eliminate the risk from antibiotic resistant and pathogenic bacteria originating from minimally processed ready-to-eat food.
    MeSH terms: Anti-Bacterial Agents; Bacteria; Genotype; Random Amplified Polymorphic DNA Technique; Drug Resistance, Bacterial
  16. Tung Nguyen, C.T., Son, R., Raha, A.R., Lai, O.M., Clemente Michael, W.V.L.
    The ability to detect the presence of transgenes in crop-derived foods depends on the quantity and quality of DNA obtained from a product to be analyzed. The efficiency of DNA extraction protocols differs due to the nature of each food product. In this paper, we described two main DNA extraction protocols and their modifications that have been applied and evaluated for DNA extraction from raw and processed food as well as animal feed. The yield and quality for five categories of food and feed samples namely, raw soybean, raw maize, animal feed, smooth tofu and soymilk are discussed. The statistical interaction analyses showed that the cetyltrimethyl ammonium bromide (CTAB) method was proven to be the best method to extract DNA from raw soybean, maize and animal feed samples which not only obtained high DNA yield of 32.7, 28.4 and 33.4 ng DNA/mg sample respectively, but also produced high quality DNA with the absorbance A260/A280 ratio of 1.9, 1.9 and 2.0, respectively. These DNA were suitable for PCR amplification which produced a 164 bp DNA fragment of the lectin gene from soybean, and a 277 bp DNA fragment of the zein gene from maize. In the processed food category, the Wizard isolation method was found to be the best for the extraction of DNA from smooth tofu and soymilk with the yield of 13.2 and 3.4 ng DNA/mg sample, and the quality of the DNA at the absorbance A260/A280 ratio ranged from 1.9 to 1.7. These DNA were successfully amplified using primers specific to the lectin gene of soybean.
    MeSH terms: Animals; Animal Feed; Cetrimonium Compounds; Zea mays; DNA; Soybeans; Zein; Polymerase Chain Reaction; DNA Primers; Transgenes; Lectins; Soy Milk; Soy Foods
  17. Behjat, T., Russly, A.R., Luqman, C.A., Yus, A.Y., Nor Azowa, I.
    Several blends of cellulose derived from bast part of kenaf (Hibiscus cannabinus L.) plant, with different thermoplastics, low density polyethylene (LDPE) and high density polyethylene (HDPE), were prepared by a melt blending machine. Polyethylene glycol (PEG) was used as plasticizer. Biodegradability of these blends was measured using soil burial test in order to study the rates of biodegradation of these polymer blends. It was found that the cellulose/LDPE and cellulose/HDPE blends were biodegradable in a considerable rate. The bio-composites with high content of cellulose had higher degradation rate. In addition, biodegradability of the bio-composites made up using PEG was superior to those of the bio-composites fabricated without PEG, due to the improved wetting of the plasticizer in the matrix polymer. The results were also supported by the scanning electron microscopy (SEM).
    MeSH terms: Biodegradation, Environmental; Burial; Cellulose; Microscopy, Electron, Scanning; Plasticizers; Polyethylene Glycols; Polymers; Soil; Wettability; Polyethylene; Hibiscus
  18. Phebe, D., Chew, M.K., Suraini, A.A., Lai, O.M., Janna, O.A.
    Red-fleshed pitaya fruit is a potential fruit for betacyanins extraction. However, there is lack of report on profiles and total contents of betacyanins in the peel and flesh. The objectives of this study were to determine colour, total betacyanins content and its separation in the peel and flesh of red-fleshed pitaya fruit harvested at 25, 30 and 35 days after flower anthesis (DAA) and to examine the usefulness of tristimulus colour measurement as predictors of pigment content in red-fleshed pitaya fruits. There were significant relationships between DAA and colour (L*, C* and h° values), and total betacyanins contents of peel and flesh of red-fleshed pitaya fruit. A total of three types betacyanins were separated from peel and flesh of pitaya fruit at 30 and 35 DAA while for 25 DAA, only one type of betacyanins was separated. The total concentration of betacyanins in the fruit peel of 25, 30 and 35 DAA was 0.24, 3.99 and 8.72 mg/mL, respectively. The fruit flesh contains 2.40, 7.93 and 11.70 mg/mL betacyanins at 25, 30 and 35 DAA, respectively, which was higher than peel. The tristimulus measurements can be adequately used to estimate the total betacyanins content of peel and flesh of red-fleshed pitaya fruit instead of tedious pigment extraction methods.
    MeSH terms: Color; Flavoring Agents; Fruit; Cactaceae; Flowers; Betacyanins; Apathy
  19. Yazdan. M,, Jamilah, B., Yaakob C.M., Sharifah, K.
    Effects of breading materials and deep-fat frying on fat uptake, moisture content and fatty acid composition of the black pomfret (Parastromateus niger) fillets. Black pomfret fillets both breaded and non-breaded were deep-fat fried in sunflower oil and palm olein. Fat uptake, moisture content and fatty acid composition in the fillets were determined. Total fat content in the fillets of both breaded and un-breaded fillets increased significantly (p
    MeSH terms: Fatty Acids; Niger; Plant Oils; Seafood
  20. Nor-Khaizura, M.A.R., Zaiton, H, Jamilah, B., Gulam Rusul, R.A.
    Keropok lekor is an important fish product in Malaysia. The customer demands for keropok lekorhave been increasing. This study was conducted to analyze the microbiological quality of keropok lekor in every stage of its processing, namely mincing, mixing, kneading, boiling and cooling. When processing keropok lekor, the boiling of keropok lekor at 100°C for 10 min reduced the Total Plate Counts (4.38±0.47 log10 cfu/g), psychrotrophic counts (2.00 ± 0.00 log10 cfu/g), mesophilic sporeformer counts (1.26 ± 0.34 log10 cfu/g) and total coliform counts (1.71±0.51 log Most Probable Number/g) significantly (p>0.05). However, the microbial counts were found to increase significantly (p
    MeSH terms: Animals; Fish Products; Malaysia; Colony Count, Microbial
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