Browse publications by year: 2012

  1. Ng ZX, Kuppusamy UR, Tajunisah I, Fong KC, Chua KH
    J Diabetes Complications, 2012 Sep-Oct;26(5):388-92.
    PMID: 22795339 DOI: 10.1016/j.jdiacomp.2012.05.014
    PURPOSE:
    In this study, we aimed to investigate the possible association between SLC2A1 26177A/G polymorphism and diabetic retinopathy (DR) in Malaysian patients with type 2 diabetes.

    METHODS:
    Genomic DNA was extracted from 211 Malaysian type 2 diabetic patients (100 without retinopathy [DNR], 111 with retinopathy) and 165 healthy controls. A high resolution melting assay developed in this study was used to detect SLC2A1 26177A/G polymorphism followed by statistical analysis.

    RESULTS:
    A statistically significant difference in 26177G minor allele frequency between healthy controls (19.7 %) and total patient group (26.1 %) (p<0.05, Odd ratio = 1.437, 95% Confidence interval = 1.015-2.035) as well as between healthy controls (19.7 %) and DNR patients (27.5%) (p<0.05, Odd ratio = 1.546, 95% Confidence interval = 1.024-2.336) was shown in this study. However, when compared between DR and DNR patients, there was no significant difference (p>0.05).

    CONCLUSIONS:
    This is the first study which shows that SLC2A1 26177G allele is associated with type 2 diabetes in Malaysian population but not with DR.
    MeSH terms: Aged; Alleles; Diabetes Mellitus, Type 2/complications; Diabetes Mellitus, Type 2/genetics*; Diabetes Mellitus, Type 2/metabolism; Diabetic Retinopathy/genetics*; Diabetic Retinopathy/metabolism; Female; Gene Frequency; Heterozygote; Homozygote; Humans; Malaysia; Male; Middle Aged; Nucleic Acid Denaturation; Polymorphism, Single Nucleotide*; Glucose Transporter Type 1/genetics*; Glucose Transporter Type 1/metabolism; Genetic Association Studies; Genotyping Techniques; Real-Time Polymerase Chain Reaction
  2. Ali S, Osman NA, Mortaza N, Eshraghi A, Gholizadeh H, Wan Abas WA
    Clin Biomech (Bristol), 2012 Nov;27(9):943-8.
    PMID: 22795863 DOI: 10.1016/j.clinbiomech.2012.06.004
    The interface pressure between the residual limb and prosthetic socket has a significant effect on an amputee's satisfaction and comfort. Liners provide a comfortable interface by adding a soft cushion between the residual limb and the socket. The Dermo and the Seal-In X5 liner are two new interface systems and, due to their relative infancy, very little are known about their effect on patient satisfaction. The aim of this study was to compare the interface pressure with these two liners and their effect on patient satisfaction.
    MeSH terms: Amputation Stumps/physiopathology*; Equipment Design; Female; Humans; Knee Joint/physiopathology*; Knee Joint/surgery*; Male; Middle Aged; Orthotic Devices*; Pressure; Tibia/physiopathology*; Tibia/surgery; Walking*; Treatment Outcome; Patient Satisfaction; Equipment Failure Analysis
  3. Ahmed MJ, Theydan SK
    Ecotoxicol Environ Saf, 2012 Oct;84:39-45.
    PMID: 22795888 DOI: 10.1016/j.ecoenv.2012.06.019
    Adsorption capacity of an agricultural waste, palm-tree fruit stones (date stones), for phenolic compounds such as phenol (Ph) and p-nitro phenol (PNPh) at different temperatures was investigated. The characteristics of such waste biomass were determined and found to have a surface area and iodine number of 495.71 m2/g and 475.88 mg/g, respectively. The effects of pH (2-12), adsorbent dose (0.6-0.8 g/L) and contact time (0-150 min) on the adsorptive removal process were studied. Maximum removal percentages of 89.95% and 92.11% were achieved for Ph and PNPh, respectively. Experimental equilibrium data for adsorption of both components were analyzed by the Langmuir, Freundlich and Tempkin isotherm models. The results show that the best fit was achieved with the Langmuir isotherm equation with maximum adsorption capacities of 132.37 and 161.44 mg/g for Ph and PNPh, respectively. The kinetic data were fitted to pseudo-first order, pseudo-second order and intraparticle diffusion models, and was found to follow closely the pseudo-second order model for both components. The calculated thermodynamic parameters, namely ΔG, ΔH, and ΔS showed that adsorption of Ph and PNPh was spontaneous and endothermic under examined conditions.
    MeSH terms: Adsorption; Hydrogen-Ion Concentration; Kinetics; Phenols/metabolism*; Seeds/metabolism*; Time Factors; Water Pollutants, Chemical/metabolism*; Water Purification*; Arecaceae/metabolism*
  4. Siddiqui MF, Sakinah M, Singh L, Zularisam AW
    J Biotechnol, 2012 Oct 31;161(3):190-7.
    PMID: 22796090 DOI: 10.1016/j.jbiotec.2012.06.029
    Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling.
    MeSH terms: Membranes, Artificial*; Pressure; Sewage/microbiology; Reproducibility of Results; Agrobacterium tumefaciens/drug effects; Agrobacterium tumefaciens/metabolism; Bioreactors/microbiology; Quorum Sensing/drug effects*; Acyl-Butyrolactones/pharmacology*; Biofouling/prevention & control*
  5. Rostami F, Yazdi SR, Said MA, Shahrokhi M
    Water Sci Technol, 2012;66(5):909-17.
    PMID: 22797216 DOI: 10.2166/wst.2012.213
    Undular hydraulic jumps are characterized by a smooth rise of the free surface, followed by a train of stationary waves. These jumps sometimes occur in natural waterways and rivers. Numerical difficulties are especially distinct when the flow condition is close to the critical value because of the high sensitivity of the near-critical flow field to flow and channel conditions. Furthermore, the free surface has a wavy shape, which may indicate the occurrence of several transitions from supercritical to subcritical states and vice versa (i.e., undular hydraulic jumps). In this study, a flow model is used to predict an undular hydraulic jump in a rectangular open channel. The model is based on the general two-dimensional, Reynolds-averaged, Navier-Stokes flow equations. The resulting set of partial differential equations is solved using the FLOW-3D solver. The results are compared with the experimental data to validate the model. The comparative analysis shows that the proposed model yields good results. Several types of undular hydraulic jumps occurring in different situations are then simulated to prove the potential application of the model.
    MeSH terms: Computer Simulation*; Models, Theoretical*; Water Movements*; Reproducibility of Results
  6. Pezeshkpour S, Khaledi H, Mohd Ali H
    Acta Crystallogr Sect E Struct Rep Online, 2012 Jul 1;68(Pt 7):o2107.
    PMID: 22798784 DOI: 10.1107/S1600536812026335
    In the Schiff base mol-ecule of the title compound, C(22)H(20)N(4)O(2)·C(3)H(7)NO·2H(2)O, the cyclo-hexane ring adopts a chair conformation with the two imine groups linked at the equatorial positions. The two indolin-2-one ring systems make a dihedral angle of 65.63 (5)°. In the crystal, the Schiff base mol-ecules are connected through bifurcated N-H⋯(O,N) hydrogen bonds, forming inversion dimers. The water molecules link the dimers and the dimethylformamide molecules via O-H⋯O, O-H⋯N and N-H⋯O hydrogen bonds. Together with C-H⋯π and π-π [centroid-centroid distance = 3.3889 (10) Å] inter-actions a three-dimensional supra-molecular structure is formed.
  7. Ng CH, Pathy NB, Taib NA, Mun KS, Rhodes A, Yip CH
    Asian Pac J Cancer Prev, 2012;13(4):1111-3.
    PMID: 22799290
    The ER-/PR+ breast tumor may be the result of a false ER negative result. The aim of this study was to investigate whether there is a difference in patient and tumor characteristics of the ER-/PR+ phenotype in an Asian setting. A total of 2629 breast cancer patients were categorized on the basis of their age, ethnicity, tumor hormonal receptor phenotype, grade and histological type. There were 1230 (46.8%) ER+/PR+, 306 (11.6%) ER+/PR-, 122 (4.6%) ER-/PR+ and 972 (37%) ER-/PR-. ER-/PR+ tumors were 2.5 times more likely to be younger than 50 years at diagnosis (OR: 2.52; 95% CI: 1.72-3.67). Compared to ER+/PR+ tumors, the ER-/ PR+ phenotype was twice more likely to be associated with grade 3 tumors (OR:2.02; 95%CI: 1.00-4.10). In contrast, compared to ER-/PR- tumors, the ER-/PR+ phenotype was 90% less likely to be associated with a grade 3 tumor (OR: 0.12; 95%CI:0.05-0.26), and more likely to have invasive lobular than invasive ductal histology (OR: 3.66; 95%CI: 1.47-9.11). These results show that the ER-/PR+ phenotype occurs in a younger age group and is associated with intermediate histopathological characteristics compared to ER+/PR+ and ER-/PR- tumors. This may imply that it is a distinct entity and not a technical artifact.
    MeSH terms: Age Factors; Analysis of Variance; Breast Neoplasms/metabolism*; Breast Neoplasms/pathology*; False Negative Reactions; Female; Humans; Malaysia; Middle Aged; Phenotype; Receptors, Estrogen/metabolism*; Receptors, Progesterone/metabolism*; Multivariate Analysis; Logistic Models; Carcinoma, Ductal, Breast/metabolism*; Carcinoma, Ductal, Breast/pathology*; Carcinoma, Lobular/metabolism*; Carcinoma, Lobular/pathology*; Neoplasm Grading
  8. Cheah YK, Naidu BM
    Asian Pac J Cancer Prev, 2012;13(4):1125-30.
    PMID: 22799293
    OBJECTIVE: The objective of present study is to investigate the determinants of smoking behaviour among adults in Malaysia.
    METHOD: Findings of the Third National Health and Morbidity Survey (NHMS-3) by the Ministry of Health, Malaysia, were used. The sample consisted of 34,539 observations. A logistic regression model was thus applied to estimate the probability to participate in smoking.
    RESULTS: Age, income, gender, marital status, ethnicity, employment status, residential area, education, lifestyle and health status were statistically significant in affecting the likelihood of smoking. Specifically, youngsters, low income earners, males, unmarried individuals, Malays, employed individuals, rural residents and primary educated individuals were more likely to smoke.
    CONCLUSION: In conclusion, socio-demographic, lifestyle and health factors have significant impacts on smoking participation in Malaysia. Based on these empirical findings, several policy implications are suggested.
    Study name: National Health and Morbidity Survey (NHMS-2006)
    MeSH terms: Adult; Age Factors; Cross-Sectional Studies; Diabetes Mellitus/epidemiology; Diet; Female; Health Status; Health Surveys; Humans; Hypercholesterolemia/epidemiology; Hypertension/epidemiology; Malaysia/epidemiology; Male; Middle Aged; Motor Activity; Risk Factors; Rural Population/statistics & numerical data; Sex Factors; Smoking/ethnology; Smoking/epidemiology*; Socioeconomic Factors; Urban Population/statistics & numerical data; Health Behavior*; Confidence Intervals; Logistic Models; Odds Ratio
  9. Mooi LY, Yew WT, Hsum YW, Soo KK, Hoon LS, Chieng YC
    Asian Pac J Cancer Prev, 2012;13(4):1177-82.
    PMID: 22799301
    Protein kinase C (PKC) has been implicated in carcinogenesis and displays variable expression profiles during cancer progression. Studies of dietary phytochemicals on cancer signalling pathway regulation have been conducted to search for potent signalling regulatory agents. The present study was designed to evaluate any suppressive effect of maslinic acid on PKC expression in human B-lymphoblastoid cells (Raji cells), and to identify the PKC isoforms expressed. Effects of maslinic acid on PKC activity were determined using a PepTag assay for non-radioactive detection of PKC. The highest expression in Raji cells was obtained at 20 nM PMA induced for 6 hours. Suppressive effects of maslinic acid were compared with those of four PKC inhibitors (H- 7, rottlerin, sphingosine, staurosporine) and two triterpenes (oleanolic acid and ursolic acid). The IC₅₀ values achieved for maslinic acid, staurosporine, H-7, sphingosine, rottlerin, ursolic acid and oleanolic acid were 11.52, 0.011, 0.767, 2.45, 5.46, 27.93 and 39.29 μM, respectively. Four PKC isoforms, PKC βI, βII, δ, and ζ, were identified in Raji cells via western blotting. Maslinic acid suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner. These preliminary results suggest promising suppressive effects of maslinic acid on PKC activity in Raji cells. Maslinic acid could be a potent cancer chemopreventive agent that may be involved in regulating many downstream signalling pathways that are activated through PKC receptors.
    MeSH terms: Acetophenones/pharmacology; Benzopyrans/pharmacology; Cells, Cultured; Enzyme Inhibitors/pharmacology*; Humans; Isoenzymes/drug effects; Isoenzymes/isolation & purification; Oleanolic Acid/pharmacology; Protein Kinase C/drug effects; Protein Kinase C/metabolism*; Sphingosine/pharmacology; Tetradecanoylphorbol Acetate/pharmacology; Triterpenes/pharmacology*; Anticarcinogenic Agents/pharmacology; 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/pharmacology; Staurosporine/pharmacology; Inhibitory Concentration 50; Precursor Cells, B-Lymphoid/drug effects*; Precursor Cells, B-Lymphoid/enzymology*
  10. Saleh A, Yang YH, Wan Abd Ghani WM, Abdullah N, Doss JG, Navonil R, et al.
    Asian Pac J Cancer Prev, 2012;13(4):1217-24.
    PMID: 22799308
    BACKGROUND AND AIM: Less than 50% of oral cancer cases are diagnosed at early stages of the disease and this is in part due to poor awareness and lack of knowledge on the signs and symptoms of oral cancer. This study sought to measure the baseline awareness of oral cancer in Malaysia and aimed to increase public awareness and knowledge of oral cancer using a mass media campaign.

    METHODS: Baseline awareness and impact of the campaign was measured using self-administered questionnaires sent via email to individuals. The campaign was aired on two national television channels and the reach was monitored through an independent programme monitoring system.

    RESULTS: 78.2% of respondents had heard of oral cancer, and this increased significantly after the campaign. However, the ability to recognize signs and symptoms remains unchanged. We found that the level of awareness differed between the distinct ethnic subgroups and the reach of the campaign was not uniform across all ethnicities.

    CONCLUSION: This substantial study to measure the oral cancer awareness in Malaysia provides important baseline data for the planning of public health policies. Despite encouraging evidence that a mass media campaign could increase the awareness of oral cancer, further research is required to address the acceptability, comprehensiveness and effectiveness. Furthermore, different campaign approaches may be required for specific ethnic groups in a multi-ethnic country such as Malaysia.

    MeSH terms: Adolescent; Adult; Child; Child, Preschool; Female; Health Education*; Health Promotion*; Humans; Health Knowledge, Attitudes, Practice/ethnology*; Malaysia; Male; Middle Aged; Mouth Neoplasms/diagnosis*; Surveys and Questionnaires; Television; Program Evaluation; Cultural Diversity*; Early Detection of Cancer*; Young Adult
  11. Jaafar H, Sharif SE, Murtey MD
    Asian Pac J Cancer Prev, 2012;13(4):1305-10.
    PMID: 22799323
    Breast cancer cells undergo transformation when they spread into surrounding tissues. Studies have shown that cancer cells undergo surface alterations and interact with the surrounding microenvironment during the invasion process. The aim of the present study was to analyse these cancer cell surface alterations and interactions of cancer cells and stroma. Twenty 1-methyl-1-nitrosourea-induced breast cancer samples taken from five rats were fixed in McDowell-Trump fixative and then washed in 0.1 M phosphate buffer. The samples were then treated with osmium tetroxide before being washed in distilled water and subsequently dehydrated through graded ethanols. The dehydrated samples were immersed in hexamethyldisilazane (HMDS), then following removal of excess HMDS, the samples were air dried at room temperature in a dessicator. The dried samples were mounted onto specimen stubs and coated with gold coater before being viewed under a scanning electron microscope. We detected the presence of membrane ruffles on the surface of cancer cells and the formation of unique surface membrane protrusions to enhance movement and adhesion to the surrounding stroma during the process of invasion. Advancing cancer cells demonstrated formation of lamellipodia and invadopodia. The stroma at the advancing edge was desmoplastic with many collagen fibres laid down near the cancer cells. Our data suggest that all of these abnormalities could act as hallmarks of invasiveness for breast cancer.
    MeSH terms: Animals; Breast Neoplasms/chemically induced; Breast Neoplasms/pathology*; Breast Neoplasms/ultrastructure*; Cell Membrane/ultrastructure*; Female; Intercellular Junctions/ultrastructure*; Methylnitrosourea; Microscopy, Electron, Scanning; Rats, Sprague-Dawley; Rats
  12. Wen CT, Hussein SZ, Abdullah S, Karim NA, Makpol S, Mohd Yusof YA
    Asian Pac J Cancer Prev, 2012;13(4):1605-10.
    PMID: 22799375
    Gelam and Nenas monofloral honeys were investigated in this study for their chemopreventive effects against HT 29 colon cancer cells. MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- tetrazolim) assays showed more effective inhibition of colon cancer cells proliferation by Gelam honey with IC₅₀ values of 39.0 mg/ml and 85.5 mg/ml respectively after 24 hours of treatment. Alkali comet assays revealed both honeys increased DNA damage significantly in a dose dependent manner. In addition, annexin V-FITC/PI flow cytometry demonstrated that at IC₅₀ concentrations and above, both Gelam and Nenas honeys induced apoptosis significantlyat values higher than for necrosis (p<0.05). Measurement of prostaglandin E₂ (PGE₂) confirmed that Gelam and Nenas honeys reduced its production in H₂O₂ inflammation-induced colon cancer cells. In conclusion, our study indicated and confirmed that both Gelam and Nenas honeys are capable of suppressing the growth of HT 29 colon cancer cells by inducing apoptosis and suppressing inflammation.
    MeSH terms: Analysis of Variance; Cell Survival; DNA Damage/drug effects; Honey*; Humans; Hydrogen Peroxide; Inflammation/chemically induced; Inflammation/metabolism; Inflammation/prevention & control*; Dinoprostone/metabolism; Anticarcinogenic Agents/pharmacology*; Anticarcinogenic Agents/therapeutic use; Apoptosis/drug effects; HT29 Cells; Inhibitory Concentration 50; Comet Assay; Cell Proliferation/drug effects
  13. Al-Dubai SA, Ganasegeran K, Alabsi AM, Abdul Manaf MR, Ijaz S, Kassim S
    Asian Pac J Cancer Prev, 2012;13(4):1627-32.
    PMID: 22799379
    BACKGROUND: Breast cancer is the most common cancer among women in Malaysia. Barriers for practicing breast self examination (BSE) await exploration.

    OBJECTIVE: To assess the practice of BSE and its correlated factors and particularly barriers amongst urban women in Malaysia.

    METHODS: This cross-sectional study was conducted with 222 Malaysian women using a self-administered questionnaire.

    RESULTS: The mean (SD) age was 28.5 (±9.2) years, 59.0% were university graduates. Of the total, 81.1% were aware of breast cancer and 55% practiced BSE. Amongst 45% of respondents who did not practice BSE, 79.8% did not know how to do it, 60.6% feared being diagnosed with breast cancer, 59.6% were worried about detecting breast cancer, 22% reported that they should not touch their bodies, 44% and 28% reported BSE is embarrassing or unpleasant, 29% time consuming, 22% thought they would never have breast cancer or it is ineffective and finally 20% perceived BSE as unimportant. Logistic regression modeling showed that respondents aged ≥45 years, being Malay, married and having a high education level were more likely to practice BSE (p<0.05).

    CONCLUSION: In this study sample, a significant proportion of respondents was aware of breast cancer but did not practice BSE. Knowledge, psychological, cultural, perception and environmental factors were identified as barriers. BSE practice was associated significantly with socio-demographic factors and socioeconomic status.
    MeSH terms: Adolescent; Adult; Age Factors; Breast Neoplasms/diagnosis*; Breast Neoplasms/ethnology; Breast Neoplasms/psychology; Cross-Sectional Studies; Culture; Educational Status; Fear; Female; Humans; Malaysia; Surveys and Questionnaires; Logistic Models; Breast Self-Examination/psychology*; Marital Status; Young Adult
  14. Oranye NO, Ahmad C, Ahmad N, Bakar RA
    Contemp Nurse, 2012 Jun;41(2):233-41.
    PMID: 22800389 DOI: 10.5172/conu.2012.41.2.233
    The objective structured clinical skills examination (OSCE) has over the years emerged as a method of evaluating clinical skills in most medical and allied professions. Although its validity and objectivity has evoked so much debate in the literature, little has been written about its application in non-traditional education systems such as in distance learning. This study examined clinical skills competence among practising nursing students who were enrolled in a distance learning programme. The study examined the effect of work and years of nursing practice on nurses' clinical skills competence.
    MeSH terms: Adult; Clinical Competence*; Education, Nursing/organization & administration*; Female; Humans; Malaysia; Male; Middle Aged; Nursing*; Professional Competence; Education, Distance*
  15. Liu YZ, Zhao X, Huang YW, Chen Z, Li FC, Gao LD, et al.
    Zhonghua Yu Fang Yi Xue Za Zhi, 2012 Mar;46(3):258-63.
    PMID: 22800599
    To investigate the gene variations of influenza B virus isolated in Hunan province from 2007 to 2010.
    MeSH terms: China/epidemiology; Genes, Viral*; Humans; Influenza, Human/epidemiology; Influenza, Human/virology*; Influenza B virus/genetics*; Influenza B virus/isolation & purification; Phylogeny; RNA, Viral; Sequence Homology
  16. Yong DJ, Iskandar H, Razif MY
    Chin Med J (Engl), 2012 May;125(9):1667-8.
    PMID: 22800842
    The significance of metastastic disease in the cervical lymph nodes has long been appreciated. The rich lymphatics of the upper aerodigestive tract explained the high incidence of cervical metastasis, occasional bilaterally spread. Even with appropriate treatment, cervical recurrences do occur. Nonetheless, with the resurgence of tuberculosis, the differential of tuberculous cervical lymphadenitis should be excluded. Appropriate modalities should be employed in making the appropriate diagnosis possible.
    MeSH terms: Aged; Antitubercular Agents/therapeutic use; Female; Humans; Lymph Nodes/microbiology*; Lymph Nodes/pathology*; Tuberculosis, Lymph Node/diagnosis*; Tuberculosis, Lymph Node/drug therapy
  17. Shameli K, Bin Ahmad M, Jaffar Al-Mulla EA, Ibrahim NA, Shabanzadeh P, Rustaiyan A, et al.
    Molecules, 2012 Jul 16;17(7):8506-17.
    PMID: 22801364 DOI: 10.3390/molecules17078506
    Different biological methods are gaining recognition for the production of silver nanoparticles (Ag-NPs) due to their multiple applications. The use of plants in the green synthesis of nanoparticles emerges as a cost effective and eco-friendly approach. In this study the green biosynthesis of silver nanoparticles using Callicarpa maingayi stem bark extract has been reported. Characterizations of nanoparticles were done using different methods, which include; ultraviolet-visible spectroscopy (UV-Vis), powder X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray fluorescence (EDXF) spectrometry, zeta potential measurements and Fourier transform infrared (FT-IR) spectroscopy. UV-visible spectrum of the aqueous medium containing silver nanoparticles showed absorption peak at around 456 nm. The TEM study showed that mean diameter and standard deviation for the formation of silver nanoparticles were 12.40 ± 3.27 nm. The XRD study showed that the particles are crystalline in nature, with a face centered cubic (fcc) structure. The most needed outcome of this work will be the development of value added products from Callicarpa maingayi for biomedical and nanotechnology based industries.
    MeSH terms: Particle Size; Silver/chemistry*; Spectrophotometry, Ultraviolet; X-Ray Diffraction; Spectroscopy, Fourier Transform Infrared; Plant Stems/chemistry*; Plant Bark/chemistry*; Callicarpa/chemistry*; Metal Nanoparticles/ultrastructure; Metal Nanoparticles/chemistry*; Static Electricity; Green Chemistry Technology/methods*
  18. Ea CK, Hao S, Yeo KS, Baltimore D
    J Biol Chem, 2012 Sep 7;287(37):31207-17.
    PMID: 22801426 DOI: 10.1074/jbc.M112.365601
    Transcriptional homeostasis relies on the balance between positive and negative regulation of gene transcription. Methylation of histone H3 lysine 9 (H3K9) is commonly correlated with gene repression. Here, we report that a euchromatic H3K9 methyltransferase, EHMT1, functions as a negative regulator in both the NF-κB- and type I interferon-mediated gene induction pathways. EHMT1 catalyzes H3K9 methylation at promoters of NF-κB target genes. Moreover, EHMT1 interacts with p50, and, surprisingly, p50 appears to repress the expression of type I interferon genes and genes activated by type I interferons by recruiting EHMT1 to catalyze H3K9 methylation at their promoter regions. Silencing the expression of EHMT1 by RNA interference enhances expression of a subset NF-κB-regulated genes, augments interferon production, and augments antiviral immunity.
    MeSH terms: Animals; Gene Expression Regulation*; Histones/genetics; Histones/immunology; Histones/metabolism; Humans; Interferon Type I/biosynthesis; Interferon Type I/genetics; Interferon Type I/immunology; Methylation; Mice, Mutant Strains; Histone-Lysine N-Methyltransferase/genetics; Histone-Lysine N-Methyltransferase/immunology; Histone-Lysine N-Methyltransferase/metabolism*; Repressor Proteins/genetics; Repressor Proteins/immunology; Repressor Proteins/metabolism*; Virus Diseases/genetics; Virus Diseases/immunology; Virus Diseases/metabolism; Mice; NF-kappa B p50 Subunit/genetics; NF-kappa B p50 Subunit/immunology; NF-kappa B p50 Subunit/metabolism*; HEK293 Cells
  19. Chowdhury SR, Aminuddin BS, Ruszymah BH
    Indian J Exp Biol, 2012 May;50(5):332-9.
    PMID: 22803323
    In the present study in vitro expansion of human keratinocytes by supplementing dermal fibroblasts conditioned medium (DFCM) has been reported. Effect of two different DFCM acquired by culturing fibroblasts in keratinocyte-specific medium (defined keratinocytes serum free medium, DFCM-DKSFM) and fibroblast-specific serum free medium (F12: DMEM nutrient mix, DFCM-FD) have been compared. Growth kinetics of keratinocytes in terms of efficiency of cell attachment, expansion index, apparent specific growth rate and growth potential at the end of culture was evaluated in culture supplemented with DFCM-DKSFM and DFCM-FD in comparison with control i.e. DKSFM only. Results indicated that supplementation of DFCM caused significant increase in keratinocyte attachment. Efficiency of keratinocyte attachment in culture supplemented with bFCM-DKSFM was significantly higher compared to those cultured in DFCM-FD and DKSFM. In addition, the expansion index of keratinocytes in cultures supplemented with DFCM-DKSFM and DFCM-FD were 3.7 and 2.2 times higher than that of control condition even though the apparent growth rate and proliferative potential was found significantly lower. These results suggested that supplementation of DFCM enhanced expansion of keratinocyte by increasing efficiency of cell attachment, and DFCM-DKSFM provided suitable condition for in vitro expansion of keratinocytes compared to DFCM-FD and control condition.
    MeSH terms: Cells, Cultured; Female; Fibroblasts/cytology*; Humans; Middle Aged; Keratinocytes/cytology*; Culture Media, Serum-Free; Culture Media, Conditioned/pharmacology*; Cell Culture Techniques; Dermis/cytology
  20. Yeow TP, Khir AS, Ismail AA, Ismail IS, Kamarul Imran M, Khalid BA, et al.
    Diabet Med, 2012 Nov;29(11):1378-84.
    PMID: 22803824 DOI: 10.1111/j.1464-5491.2012.03741.x
    AIMS: Cardiovascular disease is the foremost cause of mortality in Malaysia but little is known about the prevalence of the metabolic syndrome and its associations with other known cardiovascular risk markers. We undertook a population-based study to examine these.
    METHODS: For the study, 4341 subjects were selected using a multistage stratified sampling method. Subjects were interviewed for personal and past medical history. Biomedical markers and anthropometric indices were measured. The metabolic syndrome was defined using the harmonized criteria. The associations between the metabolic syndrome and cardiovascular risk markers, including high-sensitivity C-reactive protein, microalbuminuria and HbA(1c) were examined.
    RESULTS: The prevalence of the metabolic syndrome was 42.5%. Subjects with the metabolic syndrome are significantly more likely to have higher BMI (> 25 kg/m(2)), HbA(1c) [≥ 42 mmol/mol (6.0%)], LDL (≥ 2.6 mmol/l), elevated albumin:creatinine ratio (> 2.5 μg/mmol creatinine for men, 3.5 μg/mmol creatinine for women) and high-sensitivity C-reactive protein (> 3 mg/l); odds ratio 5.48, 6.14, 1.44, 3.68 and 1.84, respectively, P < 0.001. The presence of an elevated albumin:creatinine ratio and high-sensitivity C-reactive protein are strong predictors for the presence of a higher number of positive criteria of the metabolic syndrome. HbA(1c) > 48 mmol/mol (6.5%) is associated with increased relative risk of elevated albumin:creatinine ratio, high-sensitivity C-reactive protein and LDL (relative risk 3.10, 2.46 and 1.65 respectively, P < 0.001).
    CONCLUSIONS: We confirmed the high prevalence of the metabolic syndrome in Malaysia. Our study revealed a strong relationship between risk markers of elevated BMI, HbA(1c), LDL, albumin:creatinine ratio and high-sensitivity C-reactive protein with the presence of the metabolic syndrome, putting them at a statistically high risk for cardiovascular mortality.
    MeSH terms: Adult; C-Reactive Protein/metabolism*; Creatine/blood*; Cross-Sectional Studies; Female; Hemoglobin A, Glycosylated/metabolism*; Humans; Cholesterol, LDL/blood*; Malaysia/epidemiology; Male; Middle Aged; Predictive Value of Tests; Risk Factors; Biomarkers/blood; Body Mass Index; Prevalence; Myocardial Ischemia/blood*; Myocardial Ischemia/epidemiology; Myocardial Ischemia/prevention & control; Risk Assessment; Metabolic Syndrome X/blood*; Metabolic Syndrome X/epidemiology
External Links