Browse publications by year: 2012

  1. Székely C, Shaharom F, Cech G, Mohamed K, Zin NA, Borkhanuddin MH, et al.
    Parasitol Res, 2012 Oct;111(4):1749-56.
    PMID: 22782473
    Tor tambroides, a common and appreciated cyprinid fish of the Tasik Kenyir water reservoir in Malaysia, is one of the species selected for propagation. This fish was first successfully propagated in Malaysia by the Department of Agriculture, Sarawak, Malaysia, and the breeding program continued throughout the country. The gills were frequently infected by a Myxobolus species to be described as Myxobolus tambroides sp. n. The small, 50 to 70 μm, round plasmodia of this species is located intralamellarly. Plasmodia were filled with pyriform myxospores, 9.9 and 7.4 μm wide. In sutural view, the caudal end of the myxospores had a distinctive valvular groove, parallel with the suture. Plasmodia caused deformations on the affected and the neighbouring gill lamellae. The 18S rDNA sequence of M. tambroides sp.n. did not show a close relationship with any other Myxobolus spp., represented in the GenBank. This might be an emerging parasite likely to impact the propagation of this fish.
    MeSH terms: Animals; Cyprinidae/parasitology*; DNA, Ribosomal/genetics; DNA, Ribosomal/chemistry; Fish Diseases/parasitology*; Gills/parasitology; Malaysia; Molecular Sequence Data; Parasitic Diseases, Animal/parasitology*; Phylogeny; RNA, Ribosomal, 18S/genetics; Cluster Analysis; RNA, Protozoan/genetics; DNA, Protozoan/genetics; DNA, Protozoan/chemistry; Sequence Analysis, DNA; Genes, rRNA; Myxobolus/classification*; Myxobolus/cytology; Myxobolus/isolation & purification*
  2. Lee LH, Cheah YK, Nurul Syakima AM, Shiran MS, Tang YL, Lin HP, et al.
    Genet. Mol. Res., 2012;11(2):1627-41.
    PMID: 22782582 DOI: 10.4238/2012.June.15.12
    Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia, Rhizobium, and Staphylococcus. All isolates were studied for capability of producing antimicrobial and antifungal secondary metabolites using high-throughput screening models. Approximately 23 (13/57) and 2% (1/57) of isolates inhibited growth of Candida albicans ATCC 10231(T) and Staphylococcus aureus ATCC 51650(T), respectively. These results indicated that proteobacterium species isolates from Antarctic could serve as potential source of useful bioactive metabolites. Enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprinting produced nine clusters and 13 single isolates, with a high D value of 0.9248. RAPD fingerprinting produced six clusters and 13 single isolates, with a relatively low D value of 0.7776. ERIC-PCR analysis proved to have better discrimination capability than RAPD analysis and generated better clustering for all proteobacterium species isolates. We conclude that ERIC-PCR is a robust, reliable and rapid molecular typing method for discriminating different genera of proteobacteria.
    MeSH terms: DNA, Bacterial/genetics*; DNA Fingerprinting/methods*; Random Amplified Polymorphic DNA Technique; Bradyrhizobium/classification; Bradyrhizobium/genetics; Proteobacteria/classification; Proteobacteria/genetics*
  3. Muthusamy KA, Lian LH, Vairavan N, Chua KH, Waran V
    Genet. Mol. Res., 2012;11(3):2939-45.
    PMID: 22782629
    Studies of genetic mutations that have been used in predicting glioma prognosis have revealed a complex relationship between clinical and genetic factors. Epidermal growth factor (EGF) and the NAT2 gene play a central role in carcinogenesis. An adenine (A) to guanine (G) single nucleotide polymorphism at position 61 in the 5'-untranslated region (5'-UTR) of the EGF gene has been found to be associated with levels of EGF production, and the mutations in the NAT2 gene have been postulated as a risk factor for cancer. We investigated EGF and the NAT2 gene in 13 glioma tissue samples and 12 normal controls. In the EGF 5'-UTR 61G polymorphism, the heterozygote GA was the most common genotype in the glioma patients. In the NAT2 polymorphism at nucleotide position 857G/A, the G allele and the GG genotype were the most prevalent forms in both the glioma and normal samples. We did not find any homozygous AA genotypes in the glioma patients. Based on this preliminary evidence, the EGF 5'-UTR at position 61 and the NAT2 SNP at position 857 polymorphisms are associated with increased risk for glioma.
    MeSH terms: Alleles; Arylamine N-Acetyltransferase/genetics*; Brain Neoplasms/enzymology; Brain Neoplasms/genetics*; Electrophoresis, Agar Gel; Epidermal Growth Factor/genetics*; Gene Frequency/genetics; Glioma/enzymology; Glioma/genetics*; Humans; Malaysia; Nucleotides/genetics; Polymorphism, Restriction Fragment Length; Case-Control Studies; Polymerase Chain Reaction; Genetic Predisposition to Disease*; 5' Untranslated Regions/genetics*; Polymorphism, Single Nucleotide/genetics*; Genetic Association Studies
  4. Halim AS, Emami A, Salahshourifar I, Kannan TP
    Arch Plast Surg, 2012 May;39(3):184-9.
    PMID: 22783524 DOI: 10.5999/aps.2012.39.3.184
    Keloid disease is a fibroproliferative dermal tumor with an unknown etiology that occurs after a skin injury in genetically susceptible individuals. Increased familial aggregation, a higher prevalence in certain races, parallelism in identical twins, and alteration in gene expression all favor a remarkable genetic contribution to keloid pathology. It seems that the environment triggers the disease in genetically susceptible individuals. Several genes have been implicated in the etiology of keloid disease, but no single gene mutation has thus far been found to be responsible. Therefore, a combination of methods such as association, gene-gene interaction, epigenetics, linkage, gene expression, and protein analysis should be applied to determine keloid etiology.
    MeSH terms: Humans; Keloid; Genetic Linkage; Mutation; Neoplasms; Twins, Monozygotic; Precipitating Factors; Prevalence; Genetic Predisposition to Disease; Epigenesis, Genetic
  5. Lua PL, Zakaria NS
    J Altern Complement Med, 2012 Jun;18(6):534-40.
    PMID: 22784340 DOI: 10.1089/acm.2010.0862
    OBJECTIVES: The objective of this study was to compile existing scientific evidence regarding the effects of essential oils (EOs) administered via inhalation for the alleviation of nausea and vomiting.

    METHODS: CINAHL, PubMed, and EBSCO Host and Science Direct databases were searched for articles related to the use of EOs and/or aromatherapy for nausea and vomiting. Only articles using English as a language of publication were included. Eligible articles included all forms of evidence (nonexperimental, experimental, case report). Interventions were limited to the use of EOs by inhalation of their vapors to treat symptoms of nausea and vomiting in various conditions regardless of age group. Studies where the intervention did not utilize EOs or were concerned with only alcohol inhalation and trials that combined the use of aromatherapy with other treatments (massage, relaxations, or acupressure) were excluded.

    RESULTS: Five (5) articles met the inclusion criteria encompassing trials with 328 respondents. Their results suggest that the inhaled vapor of peppermint or ginger essential oils not only reduced the incidence and severity of nausea and vomiting but also decreased antiemetic requirements and consequently improved patient satisfaction. However, a definitive conclusion could not be drawn due to methodological flaws in the existing research articles and an acute lack of additional research in this area.

    CONCLUSIONS: The existing evidence is encouraging but yet not compelling. Hence, further well-designed large trials are needed before confirmation of EOs effectiveness in treating nausea and vomiting can be strongly substantiated.

    MeSH terms: Antiemetics/therapeutic use*; Humans; Nausea/therapy*; Oils, Volatile/therapeutic use*; Science; Vomiting/therapy*; Patient Satisfaction; Evidence-Based Medicine; Aromatherapy*; Ginger*; Mentha piperita*
  6. Goh KL, Yoon BK
    J Dig Dis, 2012 Aug;13(8):389-92.
    PMID: 22788923 DOI: 10.1111/j.1751-2980.2012.00609.x
    Pancreatic cancer is notoriously difficult to diagnose until a late stage when curative options are no longer available. Owing to its relatively low incidence and the lack of sensitivity of current diagnostic tool, screening of pancreatic cancer in the general population is not recommended. However, in high-risk individuals, especially those with well-described genetic syndromes and a strong family history of pancreatic cancer, screening can be carried out. Detection of a lesion of the diameter 
    MeSH terms: Cholangiopancreatography, Endoscopic Retrograde; Humans; Pancreatic Neoplasms/diagnosis*; Pancreatic Neoplasms/genetics; Risk Factors; Biomarkers, Tumor/blood; Risk Assessment; Endosonography; Early Detection of Cancer*
  7. Tan LH, Lum LC, Omar SF, Kan FK
    J Clin Virol, 2012 Sep;55(1):79-82.
    PMID: 22789140 DOI: 10.1016/j.jcv.2012.06.005
    Hemophagocytic syndrome is a potentially fatal disorder. It is being increasingly reported but remained under-recognized in dengue. Most reported cases were in association with plasma leakage and shock but multi-organ impairment was also observed. We describe the time-lines of 6 cases of confirmed dengue with varying severities of hemophagocytosis. All had persistent fever, cytopenia and elevated transaminases with markedly elevated ferritin levels during and beyond the plasma leakage phase. Acute renal failure and central nervous system manifestation were observed in two patients. Morphological hemophagocytosis was demonstrated in three patients. All survivors showed clinical and biochemical resolution of hemophagocytosis indicating its transient nature. Persistence of fever and cytopenia together with multi-organ dysfunction, out of proportion to and beyond the plasma leakage phase should prompt clinicians to consider this phenomenon.
    MeSH terms: Adolescent; Adult; Transaminases/blood; Antibodies, Viral/blood; Dengue/blood; Dengue/pathology*; Female; Ferritins/blood; Fever/blood; Fever/virology; Humans; Leukopenia/blood; Leukopenia/virology; Male; Thrombocytopenia/blood; Thrombocytopenia/virology; Lymphohistiocytosis, Hemophagocytic/blood; Lymphohistiocytosis, Hemophagocytic/virology*
  8. Redzuan NS, Engkasan JP, Mazlan M, Freddy Abdullah SJ
    Arch Phys Med Rehabil, 2012 Dec;93(12):2177-83.
    PMID: 22789773 DOI: 10.1016/j.apmr.2012.06.025
    To evaluate the effectiveness of an intervention using video to deliver therapy at home for patients with stroke.
    MeSH terms: Acute Disease; Aged; Female; Humans; Male; Middle Aged; Videodisc Recording*; Caregivers/psychology*; Stroke/rehabilitation*; Physical Therapy Modalities*; Tertiary Care Centers
  9. Xiang YT, Kreyenbuhl J, Dickerson FB, Ungvari GS, Wang CY, Si TM, et al.
    Aust N Z J Psychiatry, 2012 Dec;46(12):1159-64.
    PMID: 22790175 DOI: 10.1177/0004867412453625
    This study examined the prescribing patterns of several first- (FGAs) and second-generation antipsychotic (SGAs) medications administered to older Asian patients with schizophrenia during the period between 2001 and 2009.
    MeSH terms: Aged; Asia/epidemiology; Cross-Sectional Studies; Demography; Dose-Response Relationship, Drug; Female; Hospitalization/statistics & numerical data; Humans; Health Knowledge, Attitudes, Practice; Male; Practice Patterns, Physicians'/statistics & numerical data*; Schizophrenia/drug therapy*; Schizophrenia/epidemiology; Socioeconomic Factors; Adverse Drug Reaction Reporting Systems/statistics & numerical data*; Health Impact Assessment
  10. Krishnaswamy S, Subramaniam K, Indran T, Low WY
    Asia Pac J Public Health, 2012 Jul;24(4):710-8.
    PMID: 22790355 DOI: 10.1177/1010539512453261
    Disasters, natural or man-made, bring numerous health care challenges. In any crisis, mental health programs are a requirement during both the acute and postemergency phases. In the Asian tsunami on December 26, 2004, some of the northwestern coastal areas of Malaysia, particularly the island of Penang, were affected with devastating effects on the residents. Such disasters can predispose to mental health problems among the affected people. An early mental health intervention program was carried out in Balik Pulau, Penang, an area badly affected by the tsunami. The objective of the intervention program was to identify the victims, counsel them, make referrals if necessary, and provide help and resources to prevent the development of mental health problems. Penang residents identified as tsunami victims by the local health authorities were recruited. A group of health care workers, school teachers, village authorities, and volunteers were trained to carry out the crisis intervention program by health care workers experienced in crisis interventions. A total of 299 adults participated in the crisis intervention program, with follow-up assessments being made 4 to 6 weeks later. At the follow-up assessment, 1% of the victims had a problem and they were then referred for further medical assessment. This indicates that the intervention program in the first 2 weeks after the tsunami disaster with referrals to medical services may have helped stabilize the victims.
    MeSH terms: Adolescent; Adult; Aged; Mental Disorders/prevention & control*; Disasters*; Female; Follow-Up Studies; Humans; Malaysia; Male; Mental Health Services/organization & administration*; Middle Aged; Referral and Consultation; Time Factors; Program Evaluation; Survivors/psychology*; Survivors/statistics & numerical data; Young Adult; Tsunamis*
  11. Isa ZM, Abdulhadi LM
    J Oral Sci, 2012;54(2):159-63.
    PMID: 22790408
    We investigated the relationship of the maxillary central incisors to the incisive papilla in wearers of complete dentures. First, image analyzer software was used to examine the relationship of the midpoint of the incisive papilla to the labial surface of the maxillary central incisors on occlusal photographs of 120 maxillary casts from dentate Malaysian adults. Then, an Alma denture gauge was used to identify the position of the labial surface of the maxillary central incisors in relation to the midpoint of the incisive papilla in complete dentures from 51 patients who requested replacement dentures at the Faculty of Dentistry, University of Malaya. The mean incisor distance to the incisive papilla in dentate adults was 9.59 ± 1.00 mm, while the mean incisor distance to the incisive papilla in complete dentures was 6.34 ± 1.87 mm. Thus, in our sample of edentulous patients, the anterior teeth in complete dentures were positioned approximately 3 mm closer to the incisive papilla, as compared with the position of the central incisors in natural dentition, and did not duplicate the position of the natural anterior teeth.
    MeSH terms: Adult; Cephalometry/methods; China; Denture Design*; Denture, Complete, Upper*; Epithelium/anatomy & histology; Esthetics, Dental; Female; Humans; Image Processing, Computer-Assisted/methods; Incisor/anatomy & histology*; India; Jaw, Edentulous/pathology; Malaysia; Male; Maxilla/anatomy & histology*; Palate/anatomy & histology*; Tooth, Artificial*; Photography, Dental/methods; Young Adult
  12. Talari MK, Abdul Majeed AB, Tripathi DK, Tripathy M
    Chem Pharm Bull (Tokyo), 2012;60(7):818-24.
    PMID: 22790812
    The application of nanomaterials has gained considerable momentum in various fields in recent years due to their high reactivity, excellent surface properties and quantum effects in the nanometer range. The properties of zinc oxide (ZnO) vary with its crystallite size or particle size and often nanocrystalline ZnO is seen to exhibit superior physical and chemical properties due to their higher surface area and modified electronic structure. ZnO nanoparticles are reported to exhibit strong bacterial inhibiting activity and silver (Ag) has been extensively used for its antimicrobial properties since ages. In this study, Ag doped ZnO nanoparticles were synthesized by mechanochemical processing in a high energy ball mill and investigated for antimicrobial activity. The nanocrystalline nature of zinc oxide was established by X-ray diffraction (XRD) studies. It is seen from the XRD data obtained from the samples, that crystallite size of the zinc oxide nanoparticles is seen to decrease with increasing Ag addition. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) data also supported the nanoparticle formation during the synthesis. The doped nanoparticles were subjected to antimicrobial investigation and found that both increase in Ag content and decrease in particle size contributed significantly towards antimicrobial efficiency. It was also observed that Ag doped ZnO nanoparticles possess enhanced antimicrobial potential than that of virgin ZnO against the studied microorganisms of Escherichia coli and Staphylococcus aureus.
    MeSH terms: Anti-Infective Agents/chemical synthesis*; Anti-Infective Agents/pharmacology; Anti-Infective Agents/chemistry; Escherichia coli/drug effects; Microbial Sensitivity Tests; Silver/chemistry*; Staphylococcus aureus/drug effects; Zinc Oxide/chemistry*; Metal Nanoparticles/chemistry*
  13. Willcox MD
    Clin Ophthalmol, 2012;6:919-24.
    PMID: 22791973 DOI: 10.2147/OPTH.S25168
    Pubmed and Medline were searched for articles referring to Pseudomonas keratitis between the years 2007 and 2012 to obtain an overview of the current state of this disease. Keyword searches used the terms "Pseudomonas" + "Keratitis" limit to "2007-2012", and ["Ulcerative" or "Microbial"] + "Keratitis" + "Contact lenses" limit to "2007-2012". These articles were then reviewed for information on the percentage of microbial keratitis cases associated with contact lens wear, the frequency of Pseudomonas sp. as a causative agent of microbial keratitis around the world, the most common therapies to treat Pseudomonas keratitis, and the sensitivity of isolates of Pseudomonas to commonly prescribed antibiotics. The percentage of microbial keratitis associated with contact lens wear ranged from 0% in a study from Nepal to 54.5% from Japan. These differences may be due in part to different frequencies of contact lens wear. The frequency of Pseudomonas sp. as a causative agent of keratitis ranged from 1% in Japan to over 50% in studies from India, Malaysia, and Thailand. The most commonly reported agents used to treat Pseudomonas keratitis were either aminoglycoside (usually gentamicin) fortified with a cephalosporin, or monotherapy with a fluoroquinolone (usually ciprofloxacin). In most geographical areas, most strains of Pseudomonas sp. (≥95%) were sensitive to ciprofloxacin, but reports from India, Nigeria, and Thailand reported sensitivity to this antibiotic and similar fluoroquinolones of between 76% and 90%.
    MeSH terms: Aminoglycosides; Anti-Bacterial Agents; Cephalosporins; Ciprofloxacin; Contact Lenses; Gentamicins; Japan; Keratitis; Malaysia; Nepal; Nigeria; Pseudomonas; Thailand; MEDLINE; Fluoroquinolones
  14. Abdulameer SA, Sulaiman SA, Hassali MA, Subramaniam K, Sahib MN
    Patient Prefer Adherence, 2012;6:435-48.
    PMID: 22791981 DOI: 10.2147/PPA.S32745
    Diabetes mellitus (DM) is a pandemic and chronic metabolic disorder with substantial morbidity and mortality. In addition, osteoporosis (OP) is a silent disease with a harmful impact on morbidity and mortality. Therefore, this systematic review focuses on the relationship between OP and type 2 diabetes mellitus (T2DM). Systematic reviews of full-length articles published in English from January 1950 to October 2010 were identified in PubMed and other available electronic databases on the Universiti Sains Malaysia Library Database. The following keywords were used for the search: T2DM, OP, bone mass, skeletal. Studies of more than 50 patients with T2DM were included. Forty-seven studies were identified. The majority of articles (26) showed increased bone mineral density (BMD), while 13 articles revealed decreased BMD; moreover, eight articles revealed normal or no difference in bone mass. There were conflicting results concerning the influence of T2DM on BMD in association with gender, glycemic control, and body mass index. However, patients with T2DM display an increased fracture risk despite a higher BMD, which is mainly attributable to the increased risk of falling. As a conclusion, screening, identification, and prevention of potential risk factors for OP in T2DM patients are crucial and important in terms of preserving a good quality of life in diabetic patients and decreasing the risk of fracture. Patients with T2DM may additionally benefit from early visual assessment, regular exercise to improve muscle strength and balance, and specific measures for preventing falls. Patient education about an adequate calcium and vitamin D intake and regular exercise is important for improving muscle strength and balance. Furthermore, adequate glycemic control and the prevention of diabetic complications are the starting point of therapy in diabetic patients.
    MeSH terms: Diabetes Mellitus, Type 2*; Osteoporosis*
  15. Khosravi Y, Loke MF, Chua EG, Tay ST, Vadivelu J
    ScientificWorldJournal, 2012;2012:654939.
    PMID: 22792048 DOI: 10.1100/2012/654939
    Carbapenems are the primary choice of treatment for severe Pseudomonas aeruginosa infection. However, the emergence of carbapenem resistance due to the production of metallo-β-lactamases (MBLs) is of global concern. In this study, 90 imipenem- (IPM- or IP-) resistant P. aeruginosa (IRPA) isolates, including 32 previously tested positive and genotyped for MBL genes by PCR, were subjected to double-disk synergy test (DDST), combined disk test (CDT), and imipenem/imipenem-inhibitor (IP/IPI) E-test to evaluate their MBLs detection capability. All three methods were shown to have a sensitivity of 100%. However, DDST was the most specific of the three (96.6%), followed by IP/IPI E-test interpreted based on the single criteria of IP/IPI ≥8 as positive (62.1%), and CDT was the least specific (43.1%). Based on the data from this evaluation, we propose that only IRPA with IP MIC >16 μg/mL and IP/IPI ≥8 by IP/IPI E-test should be taken as positive for MBL activity. With the new dual interpretation criteria, the MBL IP/IPI E-test was shown to achieve 100% sensitivity as well as specificity for the IRPA in this study. Therefore, the IP/IPI E-test is a viable alternative phenotypic assay to detect MBL production in IRPA in our population in circumstances where PCR detection is not a feasible option.
    MeSH terms: Anti-Bacterial Agents/pharmacology; beta-Lactamases/genetics*; beta-Lactamases/isolation & purification*; False Positive Reactions; Humans; Microbial Sensitivity Tests; Phenotype; Pseudomonas aeruginosa/drug effects*; Pseudomonas aeruginosa/enzymology*; Sensitivity and Specificity; Imipenem/pharmacology*; Drug Resistance, Bacterial/genetics*; Genotyping Techniques*
  16. Chelliah KK, Faizah MZ, Dayang AA, Bilkis AA, Shareena I, Mazli M
    Case Rep Radiol, 2012;2012:564036.
    PMID: 22792503 DOI: 10.1155/2012/564036
    Conjoined twins are rare and present a unique challenge to pediatric surgeons and radiologists. An imaging strategy to accurately define anatomic fusion, vascular anomalies, and other associated abnormalities is important for surgical planning and prognostic information. A conjoined female twin with a combined weight of 2.8 kg was born by emergency caeserean. Hence, a computed tomography scan of the thorax and entire abdomen at 1.25 mm slice thickness was performed to delineate the internal structures of the twins. CT-angiography defined specific vascular supply which determined the distribution of shared structures between the twins. An echocardiogram showed four heart chambers with atrioventricular septal defect. To further evaluate the heart chambers, the twin was planned for gated cardiac magnetic resonance imaging. Unfortunately, they succumbed 6 hours apart due to complication of septicemia. Magnetic resonance imaging and CT scan provide excellent anatomic detail, demonstrating organ position, shared viscera, and limited vascular anatomy, whilst angiography defined specific vascular supply, useful in determining the distribution of shared structures between the twins in planning for surgery.
  17. Khayoon WS, Saad B, Lee TP, Salleh B
    Food Chem, 2012 Jul 15;133(2):489-96.
    PMID: 25683424 DOI: 10.1016/j.foodchem.2012.01.010
    A simple and rapid high performance liquid chromatographic with fluorescence detection method for the determination of the aflatoxin B1, B2, G1 and G2 in peanuts, rice and chilli was developed. The sample was extracted using acetonitrile:water (90:10, v/v%) and then purified by using ISOLUTE® multimode solid phase extraction. After the pre-column derivatisation, the analytes were separated within 3.7 min using Chromolith® performance RP-18e (100-4.6mm) monolithic column. To assess the possible effects of endogenous components in the food items, matrix-matched calibration was used for the quantification and validation. The recoveries of aflatoxins that were spiked into food samples were 86.38-104.5% and RSDs were <4.4%. The method was applied to the determination of aflatoxins in peanut (9), rice (5) and chilli (10) samples. Liquid chromatography-tandem mass spectrometry analysis using triple quadruple analyser and operated in the multiple reaction monitoring modes on the contaminated samples was performed for confirmation.
    MeSH terms: Aflatoxins/analysis*; Capsicum/chemistry*; Chromatography, High Pressure Liquid/methods*; Arachis/chemistry*; Oryza/chemistry*; Silicon Dioxide; Aflatoxin B1/analysis*
  18. Sanagi MM, Abbas HH, Ibrahim WA, Aboul-Enien HY
    Food Chem, 2012 Jul 15;133(2):557-62.
    PMID: 25683433 DOI: 10.1016/j.foodchem.2012.01.036
    Dispersive liquid-liquid microextraction method based on solidification of floating organic droplet (DLLME-SFO) was developed for the analysis of triazines. As model compounds four selected triazine herbicides namely, simazine, atrazine, secbumeton and cyanazine were employed to estimate the extraction efficiency. The experimental conditions were comprehensively studied for the DLLME-SFO method. Under the use of 10 μL of 1-undecanol as extraction solvent, 100 μL of acetonitrile as disperser solvent and 5% (w/v) NaCl for 3 min the results demonstrated that the repeatability (RSD%) of the optimised DLLME-SFO method ranged from 0.03% to 5.1% and the linearity in the range of 0.01-100 ppb. Low limits of detection (0.037-0.008 ppb), and good enrichment factors (195-322) were obtained. The DLLME-SFO method applied in water and sugarcane samples showed excellent relative recoveries (95.7-116.9%) with RSDs <8.6% (n=3) for all samples.
    MeSH terms: Herbicides/analysis; Herbicides/isolation & purification*; Triazines/analysis; Triazines/isolation & purification*; Water/chemistry; Water Pollutants, Chemical/analysis; Water Pollutants, Chemical/isolation & purification*; Saccharum/chemistry*; Liquid Phase Microextraction/methods*
  19. Zainal Badari SA, Arcot J, Haron SA, Paim L, Sulaiman N, Masud J
    Ecol Food Nutr, 2012;51(4):265-99.
    PMID: 22794127 DOI: 10.1080/03670244.2012.674445
    Food variety scores (FVS) and dietary diversity scores (DDS) were estimated based on foods consumed weekly by 285 Malaysian households using a food frequency questionnaire. The scoring system of FVS and DDS was based on a scale of 0-7 and 0-6 respectively. The mean household FVS and DDS was 164.1 ± 93 and 6 ± 0.4. The age of respondents (husbands or wives; p < .01), sex (p < .05), and household food expenditure (p < .01) had a significant influence on both FVS and DDS. The food-intake pattern of Malaysian households showed that their typical diets had high protein and energy-based foods.
    MeSH terms: Adult; Age Factors; Aged; Energy Intake*; Diet*; Diet Surveys; Dietary Proteins/administration & dosage*; Family Characteristics*; Female; Food Habits*; Humans; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Sex Factors; Young Adult
External Links