Browse publications by year: 2012

  1. Jayapalan JJ, Ng KL, Razack AH, Hashim OH
    Electrophoresis, 2012 Jul;33(12):1855-62.
    PMID: 22740474 DOI: 10.1002/elps.201100608
    Diagnosis of prostate cancer (PCa) is currently much reliant on the invasive and time-consuming transrectal ultrasound-guided biopsy of the prostate gland, particularly in light of the inefficient use of prostate-specific antigen as its biomarker. In the present study, we have profiled the sera of patients with PCa and benign prostatic hyperplasia (BPH) using the gel- and lectin-based proteomics methods and demonstrated the significant differential expression of apolipoprotein AII, complement C3 beta chain fragment, inter-alpha-trypsin inhibitor heavy chain 4 fragment, transthyretin, alpha-1-antitrypsin, and high molecular weight kininogen (light chain) between the two groups of patients' samples. Our data are suggestive of the potential use of the serum proteins as complementary biomarkers to effectively discriminate PCa from BPH, although this requires further extensive validation on clinically representative populations.
    MeSH terms: Aged; Blood Proteins/analysis; Blood Proteins/metabolism; Blood Proteins/chemistry; Diagnosis, Differential; Glycoproteins/blood; Glycoproteins/metabolism; Glycoproteins/chemistry; Humans; Male; Middle Aged; Prostatic Hyperplasia/blood*; Prostatic Neoplasms/blood*; Biomarkers, Tumor/blood*; Biomarkers, Tumor/chemistry; Electrophoresis, Gel, Two-Dimensional; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Lectins/metabolism; Proteomics/methods*
  2. Lee LI, Chye TT, Karmacharya BM, Govind SK
    Parasit Vectors, 2012;5:130.
    PMID: 22741573 DOI: 10.1186/1756-3305-5-130
    Blastocystis sp. is a common intestinal parasite found in faecal sample surveys. Several studies have implicated human-to-human, zoonotic and waterborne transmissions by Blastocystis sp. However, there has been no study providing evidence interlinking these three transmissions in a community. We have previously shown a high prevalence of Blastocystis sp. subtype 4 amongst village dwellers in Bahunipati, Nepal, and the present study extends the observation to assess if the same subtype of Blastocystis sp. occurs in animals they rear and rivers they frequent.
    MeSH terms: Animals; Cross-Sectional Studies; Feces/parasitology; Humans; Nepal; Ruminants; Rural Population; Swine; Water/parasitology*; Blastocystis/classification*; Blastocystis/isolation & purification*; Rivers/parasitology*
  3. Tehrani RM, Ab Ghani S
    Biosens Bioelectron, 2012 Oct-Dec;38(1):278-83.
    PMID: 22742810 DOI: 10.1016/j.bios.2012.05.044
    A non-enzymatic glucose sensor of multi-walled carbon nanotube-ruthenium oxide/composite paste electrode (MWCNT-RuO(2)/CPE) was developed. The electrode was characterized by using XRD, SEM, TEM and EIS. Meanwhile, cyclic voltammetry and amperometry were used to check on the performances of the MWCNT-RuO(2)/CPE towards glucose. The proposed electrode has displayed a synergistic effect of RuO(2) and MWCNT on the electrocatalytic oxidation of glucose in 3M NaOH. This was possible via the formation of transitions of two redox pairs, viz. Ru(VI)/Ru(IV) and Ru(VII)/Ru(VI). A linear range of 0.5-50mM glucose and a limit of detection of 33 μM glucose (S/N=3) were observed. There was no significant interference observable from the traditional interferences, viz. ascorbic acid and uric acid. Indeed, results so obtained have indicated that the developed MWCNT-RuO(2)/CPE would pave the way for a better future to glucose sensor development as its fabrication was without the use of any enzyme.
    MeSH terms: Electrodes; Glucose/analysis*; Oxidation-Reduction; Sensitivity and Specificity; Reproducibility of Results; Biosensing Techniques/instrumentation; Ruthenium Compounds/chemistry*; Nanotubes, Carbon/chemistry*; Electrochemical Techniques/instrumentation*
  4. Hassan SA, Mijin S, Yusoff UK, Ding P, Wahab PE
    Molecules, 2012 Jun 28;17(7):7843-53.
    PMID: 22743588 DOI: 10.3390/molecules17077843
    The source and quantity of nutrients available to plants can affect the quality of leafy herbs. A study was conducted to compare quality of Cosmos caudatus in response to rates of organic and mineral-based fertilizers. Organic based fertilizer GOBI (8% N:8% P₂O₅:8% K₂O) and inorganic fertilizer (15% N, 15% P₂O₅, 15% K₂O) were evaluated based on N element rates at 0, 30, 60, 90, 120 kg h⁻¹. Application of organic based fertilizer reduced nitrate, improved vitamin C, antioxidant activity as well as nitrogen and calcium nutrients content. Antioxidant activity and chlorophyll content were significantly higher with increased fertilizer application. Fertilization appeared to enhance vitamin C content, however for the maximum ascorbic acid content, regardless of fertilizer sources, plants did not require high amounts of fertilizer.
    MeSH terms: Antioxidants/analysis*; Ascorbic Acid/analysis*; Chlorophyll/analysis; Fertilizers*; Iron/chemistry; Minerals/pharmacology*; Nitrates/analysis*; Organic Chemicals/pharmacology*; Thiobarbiturates/chemistry; Thiocyanates/chemistry; Plant Leaves/drug effects; Plant Leaves/chemistry; Asteraceae/drug effects; Asteraceae/chemistry*
  5. Ibrahim SB, Yashodhara BM, Umakanth S, Kanagasabai S
    BMJ Case Rep, 2012;2012.
    PMID: 22744241 DOI: 10.1136/bcr.02.2012.5850
    A 23-year-old pregnant woman in her second trimester of pregnancy presented with blisters on the face, abdomen and the leg. Based on the clinical presentation and skin biopsy (histopathology and direct immunofluorescence) the diagnosis of pemphigus vulgaris was established. The child born to this patient also had similar skin lesions. The lesions in the mother and the child improved after treatment. The authors report a rare case of pemphigus vulgaris in a pregnant lady and neonatal pemphigus in her child, both of whom were treated successfully.
    MeSH terms: Adult; Antifungal Agents/therapeutic use; Candidiasis/complications; Candidiasis/drug therapy; Female; Glucocorticoids/therapeutic use; Humans; Immunologic Factors/therapeutic use; Immunosuppressive Agents/therapeutic use; Infant, Newborn; Methotrexate/therapeutic use; Pemphigus/complications; Pemphigus/drug therapy*; Pemphigus/genetics; Prednisolone/therapeutic use; Pregnancy; Pregnancy Complications/drug therapy*; Immunoglobulins, Intravenous/therapeutic use; Young Adult
  6. Lee SK, Yeoh HK, Chua AS, Ngoh GC
    Water Sci Technol, 2012;66(3):620-6.
    PMID: 22744694 DOI: 10.2166/wst.2012.216
    The titrimetric method is used for on-site measurement of the concentration of volatile fatty acids (VFAs) in anaerobic treatment. In current practice, specific and interpolated pH-volume data points are used to obtain the concentration of VFA by solving simultaneous equations iteratively to convergence (denoted as SEq). Here, the least squares method (LSM) is introduced as an elegant alternative. Known concentrations of VFA (acetic acid and/or propionic acid) ranging from to 200 to 1,000 mg/L were determined using SEq and LSM. Using standard numbers of data points, SEq gave more accurate results compared with LSM. However, results favoured LSM when all data points in the range were included without any interpolation. For model refinement, unit monovalent activity coefficient (f(m) = 1) was found reasonable and arithmetic averages of dissociation constants and molecular weight of 80 mol% acetic acid were recommended in the model for VFA determination of mixtures. An accurate result was obtained with a mixture containing more VFA (butyric acid and valeric acid). In a typical VFA measurement of real anaerobic effluent, a satisfactory result with an error of 14% was achieved. LSM appears to be a promising mathematical model solver for determination of concentration of VFA in the titrimetric method. Validation of LSM in the presence of other electrolytes deserves further exploration.
    MeSH terms: Models, Chemical; Propionates/analysis*; Reproducibility of Results; Least-Squares Analysis*; Titrimetry/methods*; Acetic Acid/analysis*
  7. Nagaya D, Ramanathan S, Ravichandran M, Navaratnam V
    J Integr Neurosci, 2012 Mar;11(1):117-22.
    PMID: 22744787
    Drug addiction is an important social problem in many countries. Genetic and environmental factors contribute to the predisposition of drug addiction. Genetic variations at the μ opioid receptor (OPRM1) gene locus have been associated with opiate addiction. The present study aims to delineate the frequency of A118G allele of OPRM1 among Malaysian subjects. The frequency of A allele and G allele were 51% and 49%, respectively for addicts and about 73% and 27% respectively for healthy volunteers. The frequency of G allele was 1.77-fold higher in addicts by odds ratio calculation at 95% Cl, which indicate the G allele to be strongly associated with addiction X(2) = 15.31,P < 0.0001; odds ratio 2.51; 95% Cl (1.575-3.994), compared to healthy volunteers. A significant association was observed between A118G polymorphism in μ opioid receptor gene and drug addiction.
    MeSH terms: Genotype; Humans; Malaysia; Male; Receptors, Opioid, mu/genetics*; Substance-Related Disorders/genetics*; Genetic Predisposition to Disease/genetics*; Reverse Transcriptase Polymerase Chain Reaction; Polymorphism, Single Nucleotide*
  8. Ibrahim IR, Ibrahim MI, Al-Haddad MS
    Int J Clin Pharm, 2012 Oct;34(5):728-32.
    PMID: 22744843 DOI: 10.1007/s11096-012-9667-6
    BACKGROUND: Beyond the direct pharmacological effect of medicines, preferences and perceptions toward a particular oral solid dosage form (OSDF) play a crucial role in recovery and may reduce adherence to the prescribed treatment.

    OBJECTIVES: This study conducted to investigate the most preferred OSDF and the degree to which swallowing solid medication is an issue, to assess perceptions of the therapeutic benefits of the OSDF, and to find predictors of the most preferred OSDF.

    METHOD: A cross-sectional study, through convenience sample method, was conducted to survey consumers visiting community pharmacies in Baghdad, Iraq. Data was collected by self-administered and pre-piloted questionnaires, and analyzed using Statistical Package for Social Science. Multiple logistic regression analysis and Chi-square tests were used at alpha level = 0.05.

    RESULTS: A total of 1,000 questionnaire were included in the analysis. Of all respondents, 52.9 % preferred capsule among other OSDF and this preference varied significantly with a number of socio-demographic factors. Ease of swallowing solid medication was the main issue which resulted in preferences for a particular form. A negative perception of the therapeutic benefits of the OSDF was found among 89.1 % of the consumers. Multiple logistic regression analysis indicated that gender, ease of swallowing, and perceptions of the therapeutic benefits of the OSDF were significant predictors of capsule preferences.

    CONCLUSIONS: Given the fact that consumers are the end users of medicines and their preferences may influence response to the treatment, efforts are worthwhile by the prescribers and medicines' manufactures to understand consumers' preferences of a particular dosage form in order to achieve successful therapy outcomes.

    MeSH terms: Administration, Oral; Adolescent; Adult; Aged; Capsules/administration & dosage; Consumer Behavior*; Cross-Sectional Studies; Dosage Forms; Pharmaceutical Preparations/administration & dosage*; Female; Humans; Male; Middle Aged; Perception*; Pilot Projects; Surveys and Questionnaires; Tablets/administration & dosage; Treatment Outcome; Medication Adherence/psychology*; Young Adult
  9. Salleh SH, Hussain HS, Swee TT, Ting CM, Noor AM, Pipatsart S, et al.
    Int J Nanomedicine, 2012;7:2873-81.
    PMID: 22745550 DOI: 10.2147/IJN.S32315
    Auscultation of the heart is accompanied by both electrical activity and sound. Heart auscultation provides clues to diagnose many cardiac abnormalities. Unfortunately, detection of relevant symptoms and diagnosis based on heart sound through a stethoscope is difficult. The reason GPs find this difficult is that the heart sounds are of short duration and separated from one another by less than 30 ms. In addition, the cost of false positives constitutes wasted time and emotional anxiety for both patient and GP. Many heart diseases cause changes in heart sound, waveform, and additional murmurs before other signs and symptoms appear. Heart-sound auscultation is the primary test conducted by GPs. These sounds are generated primarily by turbulent flow of blood in the heart. Analysis of heart sounds requires a quiet environment with minimum ambient noise. In order to address such issues, the technique of denoising and estimating the biomedical heart signal is proposed in this investigation. Normally, the performance of the filter naturally depends on prior information related to the statistical properties of the signal and the background noise. This paper proposes Kalman filtering for denoising statistical heart sound. The cycles of heart sounds are certain to follow first-order Gauss-Markov process. These cycles are observed with additional noise for the given measurement. The model is formulated into state-space form to enable use of a Kalman filter to estimate the clean cycles of heart sounds. The estimates obtained by Kalman filtering are optimal in mean squared sense.
    MeSH terms: Algorithms*; Heart Murmurs/physiopathology; Heart Sounds/physiology; Humans; Phonocardiography/methods*; Signal Processing, Computer-Assisted*; Signal-To-Noise Ratio
  10. Lim TM, Ibrahim MI
    Pharm Pract (Granada), 2012 Jul;10(3):136-42.
    PMID: 24155829
    Drug selection methods with scores have been developed and used worldwide for formulary purposes. These tools focus on the way in which the products are differentiated from each other within the same therapeutic class. Scoring Analytical Tool (SAT) is designed based on the same principle with score and is able to assist formulary committee members in evaluating drugs either to add or delete in a more structured, consistent and reproducible manner.
  11. Zakaria MH, Amin SM, Rahman MA, Arshad A, Christianus A, Siraj SS
    Pak J Biol Sci, 2012 Jul 01;15(13):604-9.
    PMID: 24218929
    The freshwater fish, Probarbus jullieni (Sauvage), locally referred to as "Temoleh", is a high-valued freshwater fish in Malaysia and has both cultural and conservational significance. It is widely distributed in the North-east Asian countries such as Thailand, Cambodia, Vietnam and Malaysia. During the recent past, the natural stocks of P. jullieni have been decreased severely due to habitat degradation and man-induced hazards in aquatic ecosystem. Despite the vast research that has been conducted on various carp species, little attention has been given to P. jullieni. This study reviewed the published information on the status, distribution, reproduction and biodiversity of this commercially important fish species. The findings would greatly be helpful towards the species conservation and aquaculture development of the highly endangered P. jullieni.
    MeSH terms: Animals; Conservation of Natural Resources*; Cyprinidae/physiology*; Malaysia; Reproduction; Ecosystem; Biodiversity
  12. Tan AN, Christianus A, Shakibazadeh S, Hajeb P
    Pak J Biol Sci, 2012 Jul 01;15(13):610-20.
    PMID: 24218930
    Local and regional decline of Asian horseshoe crabs has spurred a study on its spawning population at Balok Beach, Kuantan, Pahang, Malaysia. This location was identified as spawning site due to the occurrence of horseshoe crab spawning pairs and nests. Size-frequency, length-weight relationships, sex ratio and epibiont infestation of Tachypleus gigas were studied. Instar stage was estimated based on prosomal width. Condition of the horseshoe crab carapace was reported. Visual search technique of horseshoe crab was conducted during high tide of new and full moons. Prosomal, opisthosomal and telson length and weight of each horseshoe crab were measured. Largest female was recorded with mean prosomal length and width of 154.4 and 246.9 mm, respectively. About 69.8% of the males belonged to size group of 151-200 mm and 53.3% of females were grouped into 201-250 mm. All individuals were of fourteenth to sixteenth instar stages. Sex ratio varied from 0.313 to 2.5 and attributed to commercial harvest and monsoon season. Sand sediment of study site showed 93% of fine sands with grain size ranged from 120 to 250 microm. Acorn and pedunculate barnacle, conical and flat slipper shells were found on the carapace of the specimens. Most males had damaged eyes and carapaces while females with broken telsons. Body damages of about 19.9% on the specimens were likely due to nearby fishing activities. Lack of satellite male indicated low spawning population. The finding of this study showed that the species is extremely threatened by human activities and coastal development.
    MeSH terms: Animals; Female; Horseshoe Crabs/physiology*; Malaysia; Male; Reproduction; Seasons; Sex Ratio
  13. Nordiah B, Harah ZM, Sidik BJ, Hazma WN
    Pak J Biol Sci, 2012 Jul 01;15(13):621-8.
    PMID: 24218931
    Azolla pinnata R.Br. growth performance experiments in different water sources were conducted from May until July 2011 at Aquaculture Research Station, Puchong, Malaysia. Four types of water sources (waste water, drain water, paddy field water and distilled water) each with different nutrient contents were used to grow and evaluate the growth performance of A. pinnata. Four water sources with different nutrient contents; waste, drain, paddy and distilled water as control were used to evaluate the growth performance of A. pinnata. Generally, irrespective of the types of water sources there were increased in plant biomass from the initial biomass (e.g., after the first week; lowest 25.2% in distilled water to highest 133.3% in drain water) and the corresponding daily growth rate (3.61% in distilled water to 19.04% in drain water). The increased in biomass although fluctuated with time was consistently higher in drain water compared to increased in biomass for other water sources. Of the four water sources, drain water with relatively higher nitrate concentration (0.035 +/- 0.003 mg L(-l)) and nitrite (0.044 +/- 0.005 mg L(-1)) and with the available phosphate (0.032 +/- 0.006 mg L(-1)) initially provided the most favourable conditions for Azolla growth and propagation. Based on BVSTEP analysis (PRIMER v5), the results indicated that a combination of more than one nutrient or multiple nutrient contents explained the observed increased in biomass of A. pinnata grown in the different water sources.
    MeSH terms: Nitrates/metabolism; Phosphorus/metabolism; Water*; Biomass; Tracheobionta/growth & development*
  14. Abdul Rahim A, Idris MH, Kamal AH, Wong SK, Arshad A
    Pak J Biol Sci, 2012 Jul 01;15(13):629-34.
    PMID: 24218932
    The Condition Index (CI) is a method to measure overall health of fish and that has been applied to estimate the effect that different environmental factors have on clam meat quality. The CI of local mangrove clam Polymesoda expansa in Kelulit, Miri Sarawak was determined from October 2010 to November 2011. Condition index that is generally used to characterize the physiological activity of organisms, varied from 1.8% in December 2010 to 3.4% in October 2011, with low values observed during the spawning period. The clam attained their best condition in quality of flesh weight during July-October. In present study, the CI showed a clear relationship with the reproductive cycle of P. expansa. However, no significant correlation (p > 0.05) was found between CI and the different physicochemical parameter of seawater. The data presented is necessary for developing sustainable management strategies and broodstock selection for the species which is crucial in aquaculture development.
    MeSH terms: Animals; Chlorophyll/metabolism; Oxygen/metabolism; Sodium Chloride; Temperature; Bivalvia/physiology*
  15. Lai JI, Yusoff FM, Shariff M
    Pak J Biol Sci, 2012 Jul 01;15(13):635-40.
    PMID: 24218933
    Outdoor mass culture of microalgae in the tropical area is important to minimize its production cost. This study evaluates the growth of Chaetoceros calcitrans in 120 L annular photobioreactors at indoor temperature (Treatment I, 25 +/- 2 degrees C) and outdoor tropical ambient temperature, (Treatment II, 30 +/- 6 degrees C). Each treatment was done in duplicates. For both treatments, C. calcitrans was first grown in starter columns of 10 L capacity for a period of 7 days at 25 +/- 2 degrees C. After 7 days, the 9 L culture was transferred to the annular photobioreactors and subsequently brought to a final volume of 100 L by adding 20 L fresh medium every 5 days. There was no significant difference (p > 0.05) in the dry weight of microalgae grown in natural light and those grown indoor. The results suggest that C. calcitrans can be grown in outdoor conditions, hence, saving time and microalgae production cost for the larviculture industry.
    MeSH terms: Temperature*; Tropical Climate*; Bioreactors*; Photochemical Processes*; Microalgae/growth & development*
  16. Abu Hena MK, Kohinoor SM, Siddique MA, Ismail J, Idris MH, Amin SM
    Pak J Biol Sci, 2012 Jul 01;15(13):641-6.
    PMID: 24218934
    Macrobenthos in coastal environment that play a significant role in the food web. It could also use as a good indicator of aquatic ecosystem health. The abundance and composition of macrobenthos in Bakkhali channel system, Cox's Bazar were conducted in relation to the soil parameters. Samples were collected using Ekman Berge bottom grab from five different stations of Bakkhali channel. Macrobenthos were comprised of five major groups namely Polychaeta (9.96-30.31%), Oligochaeta (3.68-59.707%), Crustacea (0.02-58.40%), Bivalvia (1.40-82.09%) and Gastropoda (0.08-4.25%). Total number of macrobenthos was higher at station I (9000 individuals m(-2)) and station II (8517 individuals m(-2)) compared to other stations. Shannon diversity index among the stations ranged from 0.65-1.04. Soil pH and soil moisture ranged from 6.1-6.4 and 23.44-31.29%, respectively. The highest organic carbon concentration was observed at station I (2.11%) and lowest at station III (1.40%). Maximum fraction of sand by weight was found at stations II (81.88%) and III (87.88) while the highest fraction of clay (21.52%) and silt (8.0%) were recorded in station I. It was observed that benthic bivalves were positively correlated (r = 0.891, p > 0.05) with silt fraction of the sediments.
    MeSH terms: Animals; Soil*; Ecosystem*; Food Chain*; Estuaries
  17. Johan I, Maznah WO, Mashhor M, Abu Hena MK, Amin SM
    Pak J Biol Sci, 2012 Jul 01;15(13):647-52.
    PMID: 24218935
    Investigation on copepod communities in Perai river estuary was conducted from November 2005 to May 2006. Five stations were established for monthly sampling and were located from the river mouth to the upper reaches of the river. Copepod samples were collected from vertical tows using a standard zooplankton net. The Perai river estuary was slightly stratified and salinity decreases significantly from the mouth of the river towards the upper reaches of the river. A total of 28 species of copepods were recorded and comprised of 14 families, Paracalanidae, Oithonidae, Corycaeidae, Acartiidae, Calanidae, Centropagidae, Eucalanidae, Pontellidae, Pseudodiaptomidae, Tortanidae, Ectinosomatidae, Euterpinidae, Clausidiidae and Cyclopidae. A total of 10 species showed high positive affiliation towards salinity (R > 0.60), Acartia spinicauda, Euterpina acutifrons, Microsetella norvegica, Oithona nana, Oithona simplex, Paracalanus crassirostris, Paracalanus elegans, Paracalanus parvus, Pseudodiaptomus sp. and Hemicyclops sp. The copepod species Pseudodiaptomus dauglishi were negatively affiliated towards salinity (R = -0.71). The copepod assemblages classified into two distinct groups according to salinity regimes, euryhaline-polyhaline group (25 marine affiliated species) and oligohaline-mesohaline group (3 freshwater affiliated species).
    MeSH terms: Animals; Malaysia; Sodium Chloride; Copepoda/growth & development*; Copepoda/physiology
  18. Mirani OI, Harah ZM, Sidik BJ
    Pak J Biol Sci, 2012 Jul 01;15(13):653-7.
    PMID: 24218936
    Trapa bispinosa is a freshwater macrophyte occurring in stagnant or slow moving water streams. Information on assessment of seeds storage and germination of Trapa bispinosa is less available in literature. Dependence on fresh seeds abundance only from natural environment for plant propagation or cultivation may lead to insufficient supply of seeds due to various biotic and abiotic factors. This study evaluated the viability and germination of Trapa bispinosa seeds stored in zip-lock plastic bag at low temperature of 7 degrees C for six months and fresh seeds. In addition germinating seeds progressive development to juvenile plants was recorded and described. Experiments were conducted where stored and fresh seeds were soaked in 62 x 45 x 54 cm glass tanks filled with aged tap water to the level of 15 cm depth. Stored seeds showed low percentage germination of 2.82% compared to fresh seeds which was 71.19%. Eight distinct developmental stages were identified from germinating seeds to juvenile plants. Both stored and fresh seeds produced plants of similar morphology but stored seeds progressive development from germination, seedling to juvenile plants needed longer duration to achieve. The storage of seed at low temperature at 7 degrees C for six months showed reduced viability and also vigorousity. Improved methods should be developed for Trapa seeds storage taking into account of the seeds' endurance to dryness and moisture levels in order to maintained seeds viability for future uses either for production, research purposes or even conservation and restoration programs.
    MeSH terms: Germination*; Plant Development*
  19. Lee MY, Teh NC, Nur Zulekha M, Thayanithi S, Jelinar MN, Rizal AM, et al.
    PMID: 26107474 DOI: 10.1097/APO.0b013e31825c5b1b
    PURPOSE: To compare the effects of fixed combination of bimatoprost-timolol and travoprost-timolol on intraocular pressure (IOP) in patients with primary open-angle glaucoma or ocular hypertension.

    DESIGN: This was a prospective, randomized, observer-masked, crossover parallel comparison trial.

    METHODS: Forty-one patients with primary open-angle glaucoma or ocular hypertension on nonfixed combination of latanoprost and timolol with IOP of 21 mm Hg or less were randomized to either bimatoprost-timolol or travoprost-timolol fixed combinations for 8-week treatment period. Intraocular pressure was measured at 8 AM, 12 PM, 4 PM, and 8 PM at the baseline and at the end point. Conjunctiva hyperemia and superficial punctate keratopathy after treatment with each fixed combination therapy were assessed and compared with the baseline. Patients were then switched to the opposite drug without a medication-free period for another 8-week, and diurnal IOP measurement was repeated.

    RESULTS: Bimatoprost-timolol fixed combination reduced the baseline mean diurnal IOP statistically significantly from 17.3 mm Hg [95% confidence interval (CI), 16.8-17.7 mm Hg] to 16.4 mm Hg (95% CI, 15.9-17.0 mm Hg) (P = 0.036). Travoprost-timolol fixed combination lowered the mean diurnal IOP to 17.1 mm Hg (95% CI, 16.5-17.7 mm Hg), but it was not significant. Direct comparison between the 2 fixed combinations showed no significant difference. Both fixed combinations had no significant effect on conjunctiva hyperemia. Interestingly, patients on travoprost-timolol fixed combination had significantly less superficial punctuate keratopathy (P = 0.012).

    CONCLUSIONS: Both fixed combination of bimatoprost-timolol and travoprost-timolol had no significantly different ocular hypotensive effect. However, bimatoprost-timolol fixed combination produced additional IOP lowering in patients previously treated with nonfixed combination of latanoprost and timolol.

  20. Lee MW, Lee YC
    PMID: 26107477 DOI: 10.1097/APO.0b013e318260f730
    PURPOSE: The objective of this study was to describe our initial experience with the newest refractive platform, the Wavelight Refractive Suite.

    DESIGN: This was a consecutive case series of first 50 eyes that underwent myopic laser in situ keratomileusis.

    METHODS: A total of 50 myopic eyes (28 patients) were included in this study. Uncorrected distance visual acuity, manifest refraction, and keratometry readings were taken preoperatively and 3 months postoperatively. Emmetropia was targeted with an optical zone of 6.5 mm, and flap dimensions were of 130-μm thickness and 9-mm diameter with a superior hinge in all eyes. Flap thickness was measured with optical coherence tomography postoperatively.

    RESULTS: At 3 months postoperatively, 82% (41/50) of eyes achieved uncorrected distance visual acuity of 6/6 or better, 98% (49/50) achieved 6/9 or better, and all eyes achieved 6/12 or better. Eighty-four percent of eyes were within 0.5 diopter (D) of target of emmetropia, and 98% of eyes were within 1 D. Mean postoperative flap thickness was 124 ± 7.8 μm.

    CONCLUSIONS: The Wavelight Refractive Suite consisting of the femtosecond laser FS200 and Excimer laser EX500 is the newest integrated refractive surgical platform available and can offer good refractive predictability as shown by the results in our initial 50 myopic eyes.

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