Browse publications by year: 2013

  1. Ezat SW, Hod R, Mustafa J, Mohd Dali AZ, Sulaiman AS, Azman A
    Asian Pac J Cancer Prev, 2013;14(5):2991-9.
    PMID: 23803068
    BACKGROUND: Introduction of the HPV vaccine is a forefront primary prevention method in reducing the incidence of carcinogenic human papillomavirus (HPV) and cervical cancer. The Malaysia government has implemented the National HPV immunisation programme since 2010, supplying HPV vaccine free to targeted 13 year olds. This study aimed to explore the level of knowledge among mothers on cervical cancer, HPV, HPV vaccine and National HPV (NHPV) immunisation programme since its' implementation. It also assessed acceptance of mothers towards HPV vaccine being administered to their daughter, son or themselves.
    MATERIALS AND METHODS: A cross sectional study was conducted on 155 respondents using self-administered questionnaires; conducted in December 2012 at the Obstetrics and Gynaecology Clinic in a teaching hospital in Kuala Lumpur. Respondents were selected using a multistage sampling technique.
    RESULTS: A response rate of 100% was obtained. Overall, 51.0% of mothers had good knowledge, with 55% having good knowledge of cervical cancer, 54.2% for both HPV and the National HPV immunisation programme and 51.0% for the HPV vaccine. Regression analyses showed that ethnicity was associated with knowledge on cervical cancer (p=0.003) while education was associated with knowledge on HPV (p=0.049). Three factors are associated with knowledge of the National HPV immunisation programme; ethnicity (p=0.017), mothers' education (p=0.0005) and number of children (p=0.020). The acceptance of HPV vaccine to be administered among daughter was the highest at 87.1%, followed by for mothers themselves at 73.5%, and the least is for sons 62.6%.
    CONCLUSIONS: This study found that the overall level of knowledge was moderate. Adequate information on cervical cancer, HPV, HPV vaccination and the National HPV immunisation programme should be provided to mothers in order to increase acceptance of the HPV vaccine which can reduce the disease burden in the future.
    Study site: O&G clinic, Pusat Perubatan Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
    MeSH terms: Adult; Uterine Cervical Neoplasms/prevention & control*; Uterine Cervical Neoplasms/psychology; Uterine Cervical Neoplasms/virology; Cross-Sectional Studies; Female; Follow-Up Studies; Hospitals, Teaching; Hospitals, University; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Mothers/psychology*; National Health Programs*; Nuclear Family; Obstetrics; Outpatient Clinics, Hospital; Prognosis; Surveys and Questionnaires; Vaccination; Immunization Programs*; Papillomaviridae/immunology; Papillomavirus Infections/prevention & control*; Papillomavirus Infections/psychology; Papillomavirus Infections/virology; Papillomavirus Vaccines/therapeutic use*; Young Adult
  2. Farooqui M, Hassali MA, Knight A, Shafie AA, Farooqui MA, Saleem F, et al.
    Asian Pac J Cancer Prev, 2013;14(5):3017-21.
    PMID: 23803072
    BACKGROUND: Health Related Quality of Life (HRQoL) is an important aspect in identifying cancer patients' perceptions of being diagnosed with cancer and the assessment of treatment outcomes. The present study aimedto assess the profile and predicators of HRQoL of Malaysian oncology patients.

    MATERIALS AND METHODS: A cross sectional study adopting the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30) was conducted. All cancer patients attending Penang General Hospital between August-November 2011 were approached. Descriptive statistics were used to assess demographic and disease related characteristics of the patients. All analyses were performed using SPSS v 16.0.

    RESULTS: Three hundred and ninety three cancer patients met the inclusion criteria and were enrolled in the study. The mean age was 53.9 (SD±13) years. The cohort was dominated by females (n=260, 66.2%). Nearly half (n=190, 48.3%) of the participants were of Malay ethnicity, practicing Islam as their religion (n=194, 49.4%). Two hundred and ninety six (n=296, 75.3%) had beene diagnosed with cancer within six months to 3 years previously. The most common primary cancer site was breast (n=143, 36.4%). The mean Global Health Status (GHS) score was 60.7 (SD=21.3). Females (mean GHS score of 62.3, p=0.035) with Malay ethnicity (mean GHS score of 63.8, p=0.047), practicing Islam as their religion (mean GHS score of 63.0, p=0.011) had better GHS scores. Patients having medical insurance had good scores (mean 65.6, p=0.021). Marital status was significantly associated with GHS scores (p=0.022). Bone cancer patientshad the lowest mean GHS score of 49.2 (p=0.044). Patients at very advanced stages of cancer featured a low GHS mean score of 52.2 (p<0.001).

    CONCLUSIONS: The present study identified many demographic and disease related factors which may contribute to the HRQoL of cancer patients, pointing to the necessity for improved management of disease symptoms and provision of psychological and financial support.

    MeSH terms: Adolescent; Adult; Aged; Cross-Sectional Studies; Female; Follow-Up Studies; Health Status; Humans; Malaysia; Male; Middle Aged; Neoplasms/psychology*; Neoplasms/therapy; Prognosis; Quality of Life*; Surveys and Questionnaires; Cohort Studies; Young Adult
  3. Saad N, Esa NM, Ithnin H
    Asian Pac J Cancer Prev, 2013;14(5):3093-9.
    PMID: 23803085
    BACKGROUND: Phytic acid (PA) is a polyphosphorylated carbohydrate that can be found in high amounts in most cereals, legumes, nut oil, seeds and soy beans. It has been suggested to play a significant role in inhibition of colorectal cancer. This study was conducted to investigate expression changes of β-catenin and cyclooxygenase-2 (COX-2) and cell proliferation in the adenoma-carcinoma sequence after treatment with rice bran PA by immunocytochemistry.

    MATERIALS AND METHODS: Seventy-two male Sprague-Dawley rats were divided into 6 equal groups with 12 rats in each group. For cancer induction two intraperitoneal injections of azoxymethane (AOM) were given at 15 mg/kg bodyweight over a 2-weeks period. During the post initiation phase, two different concentrations of PA, 0.2% (w/v) and 0.5% (w/v) were administered in the diet.

    RESULTS: Results of β-catenin, COX-2 expressions and cell proliferation of Ki-67 showed a significant contribution in colonic cancer progression. For β-catenin and COX-2 expression, there was a significant difference between groups at p<0.05. With Ki-67, there was a statistically significant lowering the proliferating index as compared to AOM alone (p<0.05). A significant positive correlation (p=0.01) was noted between COX-2 expression and proliferation. Total β-catenin also demonstrated a significant positive linear relationship with total COX-2 (p=0.044).

    CONCLUSIONS: This study indicated potential value of PA extracted from rice bran in reducing colonic cancer risk in rats.

    MeSH terms: Adenoma/chemically induced; Adenoma/metabolism; Adenoma/prevention & control*; Animals; Azoxymethane/toxicity*; Carcinogens/toxicity; Colonic Neoplasms/chemically induced; Colonic Neoplasms/metabolism; Colonic Neoplasms/prevention & control*; Immunoenzyme Techniques; Male; Phytic Acid/therapeutic use*; Oryza/chemistry*; Blotting, Western; Rats, Sprague-Dawley; Apoptosis/drug effects; Cell Proliferation/drug effects; beta Catenin/antagonists & inhibitors*; beta Catenin/metabolism; Rats; Cyclooxygenase 2/metabolism; Cyclooxygenase 2/chemistry*
  4. Ho CC, Seong PK, Zainuddin ZM, Abdul Manaf MR, Parameswaran M, Razack AH
    Asian Pac J Cancer Prev, 2013;14(5):3289-92.
    PMID: 23803117
    INTRODUCTION: The purpose of this study was to identify clinical profiles of patients with low risk of having bone metastases, for which bone scanning could be safely eliminated.

    MATERIALS AND METHODS: This retrospective cross sectional study looked at prostate cancer patients seen in the Urology Departments in 2 tertiary centres over the 11 year period starting from January 2000 to May 2011. Patient demographic data, levels of PSA at diagnosis, Gleason score for the biopsy core, T-staging as well as the lymph node status were recorded and analysed.

    RESULTS: 258 men were included. The mean age of those 90 men (34.9%) with bone metastasis was 69.2 ± 7.3 years. Logistic regression found that PSA level (P=0.000) at diagnosis and patient's nodal-stage (P=0.02) were the only two independent variables able to predict the probability of bone metastasis among the newly diagnosed prostate cancer patients. Among those with a low PSA level less than 20 ng/ml, and less than 10 ng/ml, bone metastasis were detected in 10.3% (12 out of 117) and 9.7% (7 out of 72), respectively. However, by combining PSA level of 10 ng/ml or lower, and nodal negative as the two criteria to predict negative bone scan, a relatively high negative predictive value of 93.8% was obtained. The probability of bone metastasis in prostate cancer can be calculated with this formula: -1.069+0.007(PSA value, ng/ml) +1.021(Nodal status, 0 or 1)=x Probability of bone metastasis=2.718 x/1+2.718 x.

    CONCLUSION: Newly diagnosed prostate cancer patients with a PSA level of 10 ng/ml or lower and negative nodes have a very low risk of bone metastasis (negative predictive value 93.8%) and therefore bone scans may not be necessary.

    MeSH terms: Aged; Aged, 80 and over; Bone Neoplasms/blood; Bone Neoplasms/secondary*; Cross-Sectional Studies; Follow-Up Studies; Humans; Male; Middle Aged; Neoplasm Staging; Prognosis; Prostatic Neoplasms/blood; Prostatic Neoplasms/pathology*; Radiography; Retrospective Studies; Prostate-Specific Antigen/blood*; Neoplasm Grading
  5. Ezat SW, Syed Junid SM, Noraziani K, Zafar A, Saperi S, Nur AM, et al.
    Asian Pac J Cancer Prev, 2013;14(5):3357-62.
    PMID: 23803129
    The human skeleton is the most common organ to be affected by metastatic cancer and bone metastases are a major cause of cancer morbidity. The five most frequent cancers in Malaysia among males includes prostate whereas breast cancer is among those in females, both being associated with skeletal lesions. Bone metastases weaken bone structure, causing a range of symptoms and complications thus developing skeletal-related events (SRE). Patients with SRE may require palliative radiotherapy or surgery to bone for pain, having hypercalcaemia, pathologic fractures, and spinal cord compression. These complications contribute to a decline in patient health- related quality of life. The multidimensional assessment of health-related quality of life for those patients is important other than considering a beneficial treatment impact on patient survival, since the side effects of treatment and disease symptoms can significantly impact health-related quality of life. Cancer treatment could contribute to significant financial implications for the healthcare system. Therefore, it is essential to assess the health-related quality of life and treatment cost, among prostate and breast cancer patients in countries like Malaysia to rationalized cost-effective way for budget allocation or utilization of health care resources, hence helping in providing more personalized treatment for cancer patients.
    MeSH terms: Bone and Bones/pathology*; Bone Neoplasms/economics*; Bone Neoplasms/secondary*; Bone Neoplasms/therapy; Breast Neoplasms/economics; Breast Neoplasms/pathology*; Breast Neoplasms/therapy; Female; Humans; Hypercalcemia/complications; Malaysia; Male; Pain; Prostatic Neoplasms/economics; Prostatic Neoplasms/pathology*; Prostatic Neoplasms/therapy; Quality of Life; Spinal Cord Compression/complications; Health Care Costs; Fractures, Bone/complications
  6. Tubesha Z, Imam MU, Mahmud R, Ismail M
    Molecules, 2013 Jun 26;18(7):7460-72.
    PMID: 23803717 DOI: 10.3390/molecules18077460
    Toxicological studies constitute an essential part of the effort in developing an herbal medicine into a drug product. A newly developed thymoquinone-rich fraction nanoemulsion (TQRFNE) has been prepared using a high pressure homogenizer. The purpose of this study was to investigate the potential acute toxicity of this nanoemulsion in Sprague Dawley rats. The acute toxicity studies were conducted as per the OECD guidelines 425, allowing for the use of test dose limit of 20 mL TQRFNE (containing 44.5 mg TQ)/kg. TQRFNE and distilled water (DW) as a control were administered orally to both sexes of rats on Day 0 and observed for 14 days. All the animals appeared normal, and healthy throughout the study. There was no observed mortality or any signs of toxicity during the experimental period. The effects of the TQRFNE and DW groups on general behavior, body weight, food and water consumption, relative organ weight, hematology, histopathology, and clinical biochemistry were measured. All the parameters measured were unaffected as compared to the control (DW) group. The administration of 20 mL TQRFNE /kg was not toxic after an acute exposure.
    MeSH terms: Administration, Oral; Animals; Body Weight/drug effects; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Emulsions/adverse effects; Emulsions/pharmacology; Emulsions/chemistry; Female; Male; Organ Size/drug effects; Benzoquinones/adverse effects*; Benzoquinones/pharmacology*; Benzoquinones/chemistry; Rats, Sprague-Dawley; Plant Preparations/adverse effects; Plant Preparations/pharmacology; Plant Preparations/chemistry; Nigella sativa/metabolism*; Rats; Drug Discovery
  7. Kumar Potu B, Jagadeesan S, Bhat KM, Rao Sirasanagandla S
    Morphologie, 2013 Jun;97(317):31-7.
    PMID: 23806306 DOI: 10.1016/j.morpho.2013.04.004
    The retromolar foramen (RMF) and retromolar canal (RMC) are the anatomical structures of the mandible located in retromolar fossa behind the third molar tooth. This foramen and canal contain neurovascular structures which provide accessory/additional innervation to the mandibular molars and the buccal area. These neurovascular contents of the canal gain more importance in medical and dental practice, because these elements are vulnerable to damage during placement of osteointegrated implants, endodontic treatment and sagittal split osteotomy surgeries and a detailed knowledge of this anatomical variation would be vital in understanding failed inferior alveolar nerve blockage, spread of infection and also metastasis. Although few studies have been conducted in the past showing the incidence and types in different population groups, a lacunae in comprehensive review of this structure is lacking. Though this variation posed challenging situations for the practicing surgeons, it has been quite neglected and the incidence of it is not well presented in all the textbooks. Hence, we made an attempt to provide a consolidated review regarding variations and clinical applications of the RMF and RMC.
    MeSH terms: Age Factors; Arterioles/anatomy & histology; Ethnic Groups; Facial Muscles/innervation; Humans; Intraoperative Complications/prevention & control; Mandible/anatomy & histology*; Mandible/surgery; Mandibular Nerve/anatomy & histology; Molar/blood supply; Molar/innervation; Molar, Third/surgery; Nerve Fibers, Myelinated/ultrastructure; Sex Factors; Tooth Extraction/adverse effects; Venules/anatomy & histology; Oral Surgical Procedures/adverse effects; Oral Surgical Procedures/methods; Trigeminal Nerve Injuries/prevention & control
  8. Mohd Suzairi MS, Tan SC, Ahmad Aizat AA, Mohd Aminudin M, Siti Nurfatimah MS, Andee ZD, et al.
    Cancer Epidemiol, 2013 Oct;37(5):634-8.
    PMID: 23806437 DOI: 10.1016/j.canep.2013.05.007
    To investigate the allele and genotype frequencies of NFKB1 -94 ins/del ATTG (rs28720239) polymorphism and to evaluate the association between the polymorphism and colorectal cancer (CRC) risk in Malaysian population.
    MeSH terms: Adult; Aged; Aged, 80 and over; Base Sequence; Female; Gene Frequency; Genotype; Humans; Malaysia/epidemiology; Male; Middle Aged; Molecular Sequence Data; Polymorphism, Genetic; Promoter Regions, Genetic; Polymorphism, Restriction Fragment Length; Colorectal Neoplasms/genetics*; Colorectal Neoplasms/epidemiology; Case-Control Studies; Polymerase Chain Reaction; Genetic Predisposition to Disease; NF-kappa B p50 Subunit/genetics*; INDEL Mutation*; Young Adult
  9. Ng HS, Ooi CW, Mokhtar MN, Show PL, Ariff A, Tan JS, et al.
    Bioresour Technol, 2013 Aug;142:723-6.
    PMID: 23806510 DOI: 10.1016/j.biortech.2013.05.087
    An extractive bioconversion with Bacillus cereus cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) in aqueous two-phase system (ATPS) was investigated for the synthesis and recovery of cyclodextrins (CDs). Optimum condition for the extractive bioconversion of CDs was achieved in ATPS consisted of 7.7% (w/w) polyethylene glycol (PEG) 20,000 and 10.3% (w/w) dextran T500 with volume ratio (VR) of 4.0. Enzymatic conversion of starch occurred mainly in dextran-rich bottom phase whereas the product, CDs was transferred to top phase and a higher partition coefficient of CDs was achieved. Repetitive batch of CDs synthesis was employed by replenishment of the top phase components and addition of starch every 8h. An average total CDs concentration of 13.7 mg/mL, (4.77 mg/mLα-CD, 5.02 mg/mLβ-CD and 3.91 mg/mLγ-CD) was recovered in the top phase of PEG 20,000/dextran T500 ATPS. This study showed the effectiveness of ATPS application in extractive bioconversion of CDs synthesis with B. cereus CGTase.
    MeSH terms: Bacillus cereus/metabolism*; Cyclodextrins/metabolism*; Glucosyltransferases/metabolism*; Water/chemistry*
  10. Ghrici M, El Zowalaty M, Omar AR, Ideris A
    Oncol Rep, 2013 Sep;30(3):1035-44.
    PMID: 23807159 DOI: 10.3892/or.2013.2573
    Newcastle disease virus (NDV) exerts its naturally occurring oncolysis possibly through the induction of apoptosis. We hypothesized that the binding of the virus to the cell via the hemagglutinin-neuraminidase (HN) glycoprotein may be sufficient to not only induce apoptosis but to induce a higher apoptosis level than the parental NDV AF2240 virus. NDV AF2240 induction of apoptosis in MCF-7 human breast cancer cells was analyzed and quantified. In addition, the complete HN gene of NDV strain AF2240 was amplified, sequenced and cloned into the pDisplay eukaryotic expression vector. HN gene expression was first detected at the cell surface membrane of the transfected MCF-7 cells. HN induction of apoptosis in transfected MCF-7 cells was analyzed and quantified. The expression of the HN gene alone was able to induce apoptosis in MCF-7 cells but it was a less potent apoptosis inducer compared to the parental NDV AF2240 strain. In conclusion, the NDV AF2240 strain is a more suitable antitumor candidate agent than its recombinant HN gene unless the latter is further improved by additional modifications.
    MeSH terms: Animals; Breast Neoplasms/pathology*; Breast Neoplasms/therapy; Breast Neoplasms/virology; Chick Embryo; Chickens; Female; Flow Cytometry; Humans; Immunoenzyme Techniques; Newcastle Disease/pathology*; Newcastle Disease/therapy; Newcastle Disease/virology; Newcastle disease virus/growth & development; Newcastle disease virus/metabolism*; Plasmids; RNA, Messenger/genetics; Virus Replication; HN Protein/genetics; HN Protein/metabolism*; Apoptosis*; Reverse Transcriptase Polymerase Chain Reaction; Mitochondrial Membrane Transport Proteins; Oncolytic Virotherapy*; Virus Attachment; Real-Time Polymerase Chain Reaction; MCF-7 Cells
  11. Barathan M, Mariappan V, Shankar EM, Abdullah BJ, Goh KL, Vadivelu J
    Cell Death Dis, 2013;4:e697.
    PMID: 23807226 DOI: 10.1038/cddis.2013.219
    Photodynamic therapy (PDT) has emerged as a capable therapeutic modality for the treatment of cancer. PDT is a targeted cancer therapy that reportedly leads to tumor cell apoptosis and/or necrosis by facilitating the secretion of certain pro-inflammatory cytokines and expression of multiple apoptotic mediators in the tumor microenvironment. In addition, PDT also triggers oxidative stress that directs tumor cell killing and activation of inflammatory responses. However, the cellular and molecular mechanisms underlying the role of PDT in facilitating tumor cell apoptosis remain ambiguous. Here, we investigated the ability of PDT in association with hypericin (HY) to induce tumor cell apoptosis by facilitating the induction of reactive oxygen species (ROS) and secretion of Th1/Th2/Th17 cytokines in human hepatocellular liver carcinoma cell line (HepG2) cells. To discover if any apoptotic mediators were implicated in the enhancement of cell death of HY-PDT-treated tumor cells, selected gene profiling in response to HY-PDT treatment was implemented. Experimental results showed that interleukin (IL)-6 was significantly increased in all HY-PDT-treated cells, especially in 1 μg/ml HY-PDT, resulting in cell death. In addition, quantitative real-time PCR analysis revealed that the expression of apoptotic genes, such as BH3-interacting-domain death agonist (BID), cytochrome complex (CYT-C) and caspases (CASP3, 6, 7, 8 and 9) was remarkably higher in HY-PDT-treated HepG2 cells than the untreated HepG2 cells, entailing that tumor destruction of immune-mediated cell death occurs only in PDT-treated tumor cells. Hence, we showed that HY-PDT treatment induces apoptosis in HepG2 cells by facilitating cytotoxic ROS, and potentially recruits IL-6 and apoptosis mediators, providing additional hints for the existence of alternative mechanisms of anti-tumor immunity in hepatocellular carcinoma, which contribute to long-term suppression of tumor growth following PDT.
    MeSH terms: Cell Survival/drug effects; Cell Survival/radiation effects; Humans; Perylene/analogs & derivatives*; Perylene/pharmacology; Photochemotherapy; Interleukin-6/metabolism; Interleukin-6/secretion*; Up-Regulation; Gene Expression/drug effects; Gene Expression/radiation effects; Apoptosis*; Photosensitizing Agents/pharmacology*; Reactive Oxygen Species/metabolism; Inflammation Mediators/metabolism; Caspases/genetics; Caspases/metabolism*; Cell Shape; BH3 Interacting Domain Death Agonist Protein/genetics; BH3 Interacting Domain Death Agonist Protein/metabolism*; DNA Fragmentation; Hep G2 Cells
  12. Tang TH, Ahmed SA, Musa M, Zainuddin ZF
    World J Microbiol Biotechnol, 2013 Dec;29(12):2389-95.
    PMID: 23807412 DOI: 10.1007/s11274-013-1407-0
    Although the multi-copy and specific element IS6110 provides a good target for the detection of Mycobacterium tuberculosis complex by PCR techniques, the emergence of IS6110-negative strains suggested that false negative may occur if IS6110 alone is used as the target for detection. In this report, a multiplex polymerase chain reaction (mPCR) system was developed using primers derived from the insertion sequence IS6110 and an IS-like elements designated as B9 (GenBank accession no. U78639.1) to overcome the problem of detecting negative or low copy IS6110 containing strains of M. tuberculosis. The mPCR was evaluated using 346 clinical samples which included 283 sputum, 19 bronchial wash, 18 pleural fluid, 9 urine, 7 CSF, 6 pus, and 4 gastric lavage samples. Our results showed that the sensitivity (93.1 %) and specificity (89.6 %) of the mPCR system exceeds that of the conventional method of microscopy and culture. The mPCR assay provides an efficient strategy to detect and identify M. tuberculosis from clinical samples and enables prompt diagnosis when rapid identification of infecting mycobacteria is necessary.
    MeSH terms: Bronchoalveolar Lavage Fluid/microbiology; DNA Transposable Elements*; DNA, Bacterial/analysis*; Humans; Mycobacterium tuberculosis/classification; Mycobacterium tuberculosis/genetics; Mycobacterium tuberculosis/isolation & purification*; Pleural Effusion/microbiology; Sensitivity and Specificity; Sputum/microbiology; Tuberculosis/diagnosis; Tuberculosis/microbiology*; DNA Primers; Multiplex Polymerase Chain Reaction*
  13. Mahdavi M, Ahmad MB, Haron MJ, Namvar F, Nadi B, Rahman MZ, et al.
    Molecules, 2013 Jun 27;18(7):7533-48.
    PMID: 23807578 DOI: 10.3390/molecules18077533
    Superparamagnetic iron oxide nanoparticles (MNPs) with appropriate surface chemistry exhibit many interesting properties that can be exploited in a variety of biomedical applications such as magnetic resonance imaging contrast enhancement, tissue repair, hyperthermia, drug delivery and in cell separation. These applications required that the MNPs such as iron oxide Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs) having high magnetization values and particle size smaller than 100 nm. This paper reports the experimental detail for preparation of monodisperse oleic acid (OA)-coated Fe₃O₄ MNPs by chemical co-precipitation method to determine the optimum pH, initial temperature and stirring speed in order to obtain the MNPs with small particle size and size distribution that is needed for biomedical applications. The obtained nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray fluorescence spectrometry (EDXRF), thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), and vibrating sample magnetometer (VSM). The results show that the particle size as well as the magnetization of the MNPs was very much dependent on pH, initial temperature of Fe²⁺ and Fe³⁺ solutions and steering speed. The monodisperse Fe₃O₄ MNPs coated with oleic acid with size of 7.8 ± 1.9 nm were successfully prepared at optimum pH 11, initial temperature of 45°C and at stirring rate of 800 rpm. FTIR and XRD data reveal that the oleic acid molecules were adsorbed on the magnetic nanoparticles by chemisorption. Analyses of TEM show the oleic acid provided the Fe₃O₄ particles with better dispersibility. The synthesized Fe₃O₄ nanoparticles exhibited superparamagnetic behavior and the saturation magnetization of the Fe₃O₄ nanoparticles increased with the particle size.
    MeSH terms: Biocompatible Materials/administration & dosage; Biocompatible Materials/chemistry*; Drug Carriers; Ferric Compounds/administration & dosage; Ferric Compounds/chemistry*; Microscopy, Electron, Scanning; Spectroscopy, Fourier Transform Infrared; Magnetite Nanoparticles/administration & dosage; Magnetite Nanoparticles/chemistry*
  14. Swe KM, Abas AB, Bhardwaj A, Barua A, Nair NS
    Cochrane Database Syst Rev, 2013 Jun 28;2013(6):CD009415.
    PMID: 23807756 DOI: 10.1002/14651858.CD009415.pub2
    BACKGROUND: Haemoglobinopathies, inherited disorders of haemoglobin synthesis (thalassaemia) or structure (sickle cell disease), are responsible for significant morbidity and mortality throughout the world. The WHO estimates that, globally, 5% of adults are carriers of a haemoglobin condition, 2.9% are carriers of thalassaemia and 2.3% are carriers of sickle cell disease. Carriers are found worldwide as a result of migration of various ethnic groups to different regions of the world. Zinc is an easily available supplement and intervention programs have been carried out to prevent deficiency in people with thalassaemia or sickle cell anaemia. It is important to evaluate the role of zinc supplementation in the treatment of thalassaemia and sickle cell anaemia to reduce deaths due to complications.

    OBJECTIVES: To assess the effect of zinc supplementation in the treatment of thalassaemia and sickle cell disease.

    SEARCH METHODS: We searched the Cochrane Cystic Fibrosis and Genetic Disorders Group's Haemoglobinopathies Trials Register comprising references identified from comprehensive electronic database searches and handsearches of relevant journals and abstract books of conference proceedings.Date of most recent search: 01 February 2013.

    SELECTION CRITERIA: Randomised, placebo-controlled trials of zinc supplements for treating thalassaemia or sickle cell disease administered at least once a week for at least a month.

    DATA COLLECTION AND ANALYSIS: Two review authors assessed the eligibility and risk of bias of the included trials, extracted and analysed data and wrote the review. We summarised results using risk ratios or rate ratios for dichotomous data and mean differences for continuous data. We combined trial results where appropriate.

    MAIN RESULTS: We identified nine trials for inclusion with all nine contributing outcome data. Two trials reported on people with thalassaemia (n = 152) and seven on sickle cell anaemia (n = 307).In people with thalassaemia, in one trial, the serum zinc level value showed no difference between the zinc supplemented group and the control group, mean difference 47.40 (95% confidence interval -12.95 to 107.99). Regarding anthropometry, in one trial, height velocity was significantly increased in patients who received zinc supplementation for one to seven years duration, mean difference 3.37 (95% confidence interval 2.36 to 4.38) (total number of participants = 26). In one trial, however, there was no difference in body mass index between treatment groups.Zinc acetate supplementation for three months (in one trial) and one year (in two trials) (total number of participants = 71) was noted to increase the serum zinc level significantly in patients with sickle cell anaemia, mean difference 14.90 (95% confidence interval 6.94 to 22.86) and 20.25 (95% confidence interval 11.73 to 28.77) respectively. There was no significant difference in haemoglobin level between intervention and control groups, at either three months (one trial) or one year (one trial), mean difference 0.06 (95% confidence interval -0.84 to 0.96) and mean difference -0.07 (95% confidence interval -1.40 to 1.26) respectively. Regarding anthropometry, one trial showed no significant changes in body mass index or weight after one year of zinc acetate supplementation. In patients with sickle cell disease, the total number of sickle cell crises at one year were significantly decreased in the zinc sulphate supplemented group as compared to controls, mean difference -2.83 (95% confidence interval -3.51 to -2.15) (total participants 130), but not in zinc acetate group, mean difference 1.54 (95% confidence interval -2.01 to 5.09) (total participants 22). In one trial at three months and another at one year, the total number of clinical infections were significantly decreased in the zinc supplemented group as compared to controls, mean difference 0.05 (95% confidence interval 0.01 - 0.43) (total number of participants = 36), and mean difference -7.64 (95% confidence interval -10.89 to -4.39) (total number of participants = 21) respectively.

    AUTHORS' CONCLUSIONS: According to the results, there is no evidence from randomised controlled trials to indicate any benefit of zinc supplementation with regards to serum zinc level in patients with thalassaemia. However, height velocity was noted to increase among those who received this intervention.There is mixed evidence on the benefit of using zinc supplementation in people with sickle cell disease. For instance, there is evidence that zinc supplementation for one year increased the serum zinc levels in patients with sickle cell disease. However, though serum zinc level was raised in patients receiving zinc supplementation, haemoglobin level and anthropometry measurements were not significantly different between groups. Evidence of benefit is seen with the reduction in the number of sickle cell crises among sickle cell patients who received one year of zinc sulphate supplementation and with the reduction in the total number of clinical infections among sickle cell patients who received zinc supplementation for both three months and for one year.The conclusion is based on the data from a small group of trials,which were generally of good quality, with a low risk of bias. The authors recommend that more trials on zinc supplementation in thalassaemia and sickle cell disease be conducted given that the literature has shown the benefits of zinc in these types of diseases.

    MeSH terms: Anemia, Sickle Cell/blood; Anemia, Sickle Cell/therapy*; Body Height; Hemoglobin A/metabolism; Humans; Thalassemia/blood; Thalassemia/therapy*; Zinc/blood; Body Mass Index; Randomized Controlled Trials as Topic; Zinc Sulfate/administration & dosage*; Zinc Acetate/administration & dosage*
  15. Bonsu KO, Kadirvelu A, Reidpath DD
    Vasc Health Risk Manag, 2013;9:303-19.
    PMID: 23807852 DOI: 10.2147/VHRM.S44499
    Statins lower serum cholesterol and are employed for primary and secondary prevention of cardiovascular events. Clinical evidence from observational studies, retrospective data, and post hoc analyses of data from large statin trials in various cardiovascular conditions, as well as small scale randomized trials, suggest survival and other outcome benefits for heart failure. Two recent large randomized controlled trials, however, appear to suggest statins do not have beneficial effects in heart failure. In addition to lowering cholesterol, statins are believed to have many pleotropic effects which could possibly influence the pathophysiology of heart failure. Following the two large trials, evidence from recent studies appears to support the use of statins in heart failure. This review discusses the role of statins in the pathophysiology of heart failure, current evidence for statin use in heart failure, and suggests directions for future research.
    MeSH terms: Animals; Clinical Trials as Topic*; Heart Failure/drug therapy*; Heart Failure/mortality; Heart Failure/physiopathology; Humans; Treatment Outcome; Hydroxymethylglutaryl-CoA Reductase Inhibitors/adverse effects; Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use*; Evidence-Based Medicine
  16. Kwan M, Chan C, Ng W, Merican A, Chung W, Chan S
    Malays Orthop J, 2013 Jul;7(2):12-7.
    PMID: 25722819 MyJurnal DOI: 10.5704/MOJ.1307.008
    There are reported cases of cortical reaction over the tension side of the normal femoral shafts in patients on long term treatment with alendronate, leading to subsequent femoral shaft fractures. We performed a retrospective review of patients with low-energy femoral shaft fracture on alendronate, admitted to our institution during the period 2004 to May 2009. The presence of radiological changes of cortical hypertrophy with or without Looser's zone over the tension side of the femoral bone (normal limb) was determined and correlated with clinical symptoms. Thirteen patients were identified. Average duration of alendronate use was 6.5 ± 3.3 years (ranges, two to 10 years). These radiological changes were noted in four patients. Average duration of alendronate usage in these four patients was 6.5 ± 2.4 years (ranges, 5 to10 years). Prodromal thigh pain was present in a patient, who had cortical hypertrophy with the presence of a Looser's zone traversing the cortex on the femoral shaft. One patient had Looser's zone limited at the lateral hypertrophied cortex without prodromal pain. The interobserver kappa coefficient was 0.96. A femoral radiograph should be performed in all patients who are on long-term alendronate therapy who present with thigh pain. We propose a new grading system based on our observation of the radiological features in these four cases. This new grading of the radiological spectrum of femoral shaft cortical pathology has the potential to stratify the risk of low energy femoral fracture for patients treated with long-term alendronate therapy.
    MeSH terms: Femoral Fractures; Femur; Humans; Hypertrophy; Pain; Radiography; Retrospective Studies; Thigh; Diaphyses; Alendronate
  17. Lee D, Loh E, Kueh C, Poi J, Francis T, Koh KC, et al.
    Malays Orthop J, 2013 Jul;7(2):18-20.
    PMID: 25722820 MyJurnal DOI: 10.5704/MOJ.1307.009
    We set out to assess the efficacy of radiofrequency-induced intradiscal nucleoplasty in reducing pain in symptomatic patients with MRI-defined lumbar disc herniation and their satisfaction with the procedure. We compared the patients' pain intensity and severity of disability scores before and after undergoing the procedure in a retrospective questionnaire. These patients reported statistically significant reduction of pain intensity and disability level after the procedure. We conclude that radiofrequencyinduced intradiscal nucleoplasty is an acceptable alternative minimally invasive procedure in relieving the symptoms of patients with lumbar disc herniation.
    MeSH terms: Humans; Intervertebral Disc Displacement; Magnetic Resonance Imaging; Pain; Personal Satisfaction; Surveys and Questionnaires; Retrospective Studies; Intervertebral Disc Degeneration
  18. Thevarajan K, Teo P
    Malays Orthop J, 2013 Jul;7(2):37-40.
    PMID: 25722825 MyJurnal DOI: 10.5704/MOJ.1307.003
    Various methods, such as vascularized bone transfers, Illizarov bone transport, allogenic bone grafts, bone graft substitutes, are available in treating traumatic bone loss. Free non-vascularised fibular graft is an autografting method that only requires minimal facilities or expertise. However, this method is not popularized due to its avascular property and there is not many reports regarding its use in treating a large traumatic bone loss. We reported a case in our center to demonstrate its possibility of successfully treating the traumatic radial bone loss in pediatric patient. Patient had good recovery with the regain of good range of movement of forearm and there is no harvest site morbidity after two years of follow up.
    MeSH terms: Child; Fibula; Follow-Up Studies; Forearm; Humans; Radius; Transplantation, Autologous; Bone Transplantation; Bone Substitutes; Autografts
  19. Chan C, Ng W, Merican A
    Malays Orthop J, 2013 Jul;7(2):41-4.
    PMID: 25722826 MyJurnal DOI: 10.5704/MOJ.1307.015
    Non-union is a well recognized complication of femoral neck fractures. The decision whether to attempt fracture fixation or to resort to hip replacement is particularly difficult in patients in the borderline age group in whom complex attempts at gaining union may fail and later present a difficult revision. On the other hand the patient may be young enough that arthroplasty best be avoided . Besides, presence of ipsilateral femoral shaft fracture with delayed union in addition to the femoral neck non-union will pose major problems at operation. We share our experience in treating a femoral neck fracture non-union with ipsilateral femoral shaft delayed union in the shaft and in the distal femur in a fifty years old patient. The fracture was treated with an angle blade plate and supracondylar nail supplemented with a free vascularised fibular bone grafting and autologous cancellous graft. There was radiological union at fourth month. At sixth months, the patient was free of pain and able to walk without support. Thus, we would like to suggest that vascularised fibula bone grafting with supracondylar nailing is a viable option for this pattern of fracture.

    KEY WORDS: Vascularized fibular bone graft, neck of femur, femoral shaft fracture, non-union.

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