Browse publications by year: 2014

  1. Shohaimi S, Boekholdt MS, Luben R, Wareham NJ, Khaw KT
    BMC Public Health, 2014 Aug 28;14:782.
    PMID: 25179437 DOI: 10.1186/1471-2458-14-782
    BACKGROUND: Data on the relationship between plasma levels of cholesterol and triglycerides and social class have been inconsistent. Most previous studies have used one classification of social class.

    METHODS: This was a cross-sectional population based study with data on occupational social class, educational level obtained using a detailed health and lifestyle questionnaire. A total of 10,147 men and 12,304 women aged 45-80 years living in Norfolk, United Kingdom, were recruited using general practice age-sex registers as part of the European Prospective Investigation into Cancer (EPIC-Norfolk). Plasma levels of cholesterol and triglycerides were measured in baseline samples. Social class was classified according to three classifications: occupation, educational level, and area deprivation score according to Townsend deprivation index. Differences in lipid levels by socio-economic status indices were quantified by analysis of variance (ANOVA) and multiple linear regression after adjusting for body mass index and alcohol consumption.

    RESULTS: Total cholesterol levels were associated with occupational level among men, and with educational level among women. Triglyceride levels were associated with educational level and occupational level among women, but the latter association was lost after adjustment for age and body mass index. HDL-cholesterol levels were associated with both educational level and educational level among men and women. The relationships with educational level were substantially attenuated by adjustment for age, body mass index and alcohol use, whereas the association with educational class was retained upon adjustment. LDL-cholesterol levels were not associated with social class indices among men, but a positive association was observed with educational class among women. This association was not affected by adjustment for age, body mass index and alcohol use.

    CONCLUSIONS: The findings of this study suggest that there are sex differences in the association between socio-economic status and serum lipid levels. The variations in lipid profile with socio-economic status may be largely attributed to potentially modifiable factors such as obesity, physical activity and dietary intake.

    MeSH terms: Aged; Aged, 80 and over; Analysis of Variance; Cholesterol/blood; Cross-Sectional Studies; England/epidemiology; Female; Humans; Hyperlipidemias/blood; Hyperlipidemias/epidemiology*; Life Style; Male; Middle Aged; Occupations; Prospective Studies; Surveys and Questionnaires; Social Class; Body Mass Index
  2. Halim I, Arep H, Kamat SR, Abdullah R, Omar AR, Ismail AR
    Saf Health Work, 2014 Jun;5(2):97-105.
    PMID: 25180141 DOI: 10.1016/j.shaw.2014.04.002
    BACKGROUND: Prolonged standing has been hypothesized as a vital contributor to discomfort and muscle fatigue in the workplace. The objective of this study was to develop a decision support system that could provide systematic analysis and solutions to minimize the discomfort and muscle fatigue associated with prolonged standing.

    METHODS: The integration of object-oriented programming and a Model Oriented Simultaneous Engineering System were used to design the architecture of the decision support system.

    RESULTS: Validation of the decision support system was carried out in two manufacturing companies. The validation process showed that the decision support system produced reliable results.

    CONCLUSION: The decision support system is a reliable advisory tool for providing analysis and solutions to problems related to the discomfort and muscle fatigue associated with prolonged standing. Further testing of the decision support system is suggested before it is used commercially.

    MeSH terms: Expert Systems; Posture; Workplace; Muscle Fatigue; Orientation, Spatial
  3. Bagherifaez M, Behnia A, Majeed AA, Hwa Kian C
    ScientificWorldJournal, 2014;2014:567619.
    PMID: 25180203 DOI: 10.1155/2014/567619
    Reinforced concrete (RC) box girders are a common structural member for road bridges in modern construction. The hollow cross-section of a box girder is ideal in carrying eccentric loads or torques introduced by skew supports. This study employed acoustic emission (AE) monitoring on multicell RC box girder specimens subjected to laboratory-based torsion loading. Three multicell box girder specimens with different cross-sections were tested. The aim is to acquire AE analysis data indicative for characterizing torsion fracture in the box girders. It was demonstrated through appropriate parametric analysis that the AE technique could be utilized to effectively classify fracture developed in the specimens for describing their mechanical behavior under torsion. AE events localization was presented to illustrate the trend of crack and damage propagation in different stages of fracture. It could be observed that spiral-like patterns of crack were captured through AE damage localization system and damage was quantified successfully in different stages of fracture by using smoothed b-value analysis.
    MeSH terms: Acoustics/instrumentation; Construction Materials/standards*; Sound*; Torsion, Mechanical*; Structure Collapse/prevention & control
  4. Kuppusamy UR, Arumugam B, Azaman N, Jen Wai C
    ScientificWorldJournal, 2014;2014:737263.
    PMID: 25180205 DOI: 10.1155/2014/737263
    Leucaena leucocephala had been traditionally used to treat diabetes. The present study was designed to evaluate in vitro "insulin-like" activities of Leucaena leucocephala (Lam.) deWit. aqueous fruit extract on lipid and glucose metabolisms. The ability of the extract to stimulate adipogenesis, inhibit lipolysis, and activate radio-labeled glucose uptake was assessed using primary rat adipocytes. Quantitative Real-Time RT-PCR was performed to investigate effects of the extract on expression levels of genes (protein kinases B, AKT; glucose transporter 4, GLUT4; hormone sensitive lipase, HSL; phosphatidylinositol-3-kinases, PI3KA; sterol regulatory element binding factor 1, Srebp1) involved in insulin-induced signaling pathways. L. leucocephala aqueous fruit extract stimulated moderate adipogenesis and glucose uptake into adipocytes when compared to insulin. Generally, the extract exerted a considerable level of lipolytic effect at lower concentration but decreased gradually at higher concentration. The findings concurred with RT-PCR analysis. The expressions of GLUT4 and HSL genes were upregulated by twofold and onefold, respectively, whereas AKT, PI3KA, and Srebp1 genes were downregulated. The L. leucocephala aqueous fruit extract may be potentially used as an adjuvant in the treatment of Type 2 diabetes mellitus and weight management due to its enhanced glucose uptake and balanced adipogenesis and lipolysis properties.
    MeSH terms: Animals; Biological Transport, Active; Cells, Cultured; Sterol Esterase/genetics; Sterol Esterase/metabolism; Fruit/chemistry; Glucose/metabolism; Insulin/metabolism; Fabaceae/chemistry*; Lipolysis*; Male; Plant Extracts/pharmacology*; Plant Extracts/chemistry; Signal Transduction; Rats, Sprague-Dawley; Adipocytes/cytology; Adipocytes/drug effects*; Adipocytes/metabolism; Phosphatidylinositol 3-Kinases/genetics; Phosphatidylinositol 3-Kinases/metabolism; Adipogenesis*; Proto-Oncogene Proteins c-akt/genetics; Proto-Oncogene Proteins c-akt/metabolism; Glucose Transporter Type 4/genetics; Glucose Transporter Type 4/metabolism; Rats; Sterol Regulatory Element Binding Protein 1/genetics; Sterol Regulatory Element Binding Protein 1/metabolism
  5. Gaythri T, Suresh K, Subha B, Kalyani R
    PLoS One, 2014;9(9):e95608.
    PMID: 25180903 DOI: 10.1371/journal.pone.0095608
    Protistan parasites in order to ensure their viability and demonstrate successful progression in their life cycle need to respond towards various environmental stressors. Blastocystis sp. is known to be the most commonly found intestinal protistan parasite in any human stool surveys and has been incriminated to be responsible for diarrhea and bloating stomach. The present study demonstrates for the first time the presence of HSP70 in subtypes of Blastocystis sp. when the cultures were subjected to temperature of 39 and 41 °C where the growth of parasites was reduced to a minimum to majority being granular forms. The growth of parasites exposed to higher temperatures however doubled compared to the controls when the parasites were re-cultured back at 37 °C. Upon thermal stress at 41 °C, subtype 3 and subtype 5 isolates' growth reached up to 2.97 × 10(6) and 3.05 × 10(6) cells/ml compared to their respective controlled culture tubes at 37 °C which peaked only at 1.34 × 10(6) and 1.70 × 10(6) cells/ml respectively. The designed primer set that amplified Blastocystis sp. subtype 7 HSP70 gene in subtypes 1, 3 and 5 was against a conserved region. The gene was amplified at 318 bp. The multiple sequence alignment showed that the targeted sequence length ranges from 291-295 bp. The pair wise alignment result showed that the sequence identity among the four sequence ranges from 88% to 96%. These findings were further evidenced by the up regulation of HSP70 gene in thermal stressed isolates of subtype 3 and 5 at 41 °C. Higher number of granular forms was significantly found in thermal stressed isolates of subtype 3 and 5 which implicates that this life cycle stage has a role in responding to thermal stress.
    MeSH terms: Base Sequence; Gene Expression Regulation; Molecular Sequence Data; Protozoan Proteins/genetics; Protozoan Proteins/metabolism*; Blastocystis/genetics; Blastocystis/metabolism; Blastocystis/physiology*; HSP70 Heat-Shock Proteins/genetics; HSP70 Heat-Shock Proteins/metabolism*; Heat-Shock Response*
  6. Terhem RB, van Kan JA
    Fungal Genet. Biol., 2014 Oct;71:42-51.
    PMID: 25181040 DOI: 10.1016/j.fgb.2014.08.002
    Hydrophobins are small secreted fungal proteins that play roles in growth and development of filamentous fungi, i.e. in the formation of aerial structures and the attachment of hyphae to hydrophobic surfaces. In Botrytis cinerea, three hydrophobin genes have been identified. Studies by Mosbach et al. (2011) showed that hydrophobins are neither involved in conferring surface hydrophobicity to conidia and aerial hyphae of B. cinerea, nor are they required for virulence. The present study investigated the role of hydrophobins in sclerotium and apothecium development. Expression analysis revealed high expression of the Bhp1 gene during different stages of apothecium development. Two Bhp1 splice variants were detected that differ by an internal stretch of 13 amino acid residues. Seven different mutants in which either a single, two or three hydrophobin genes were knocked out, as well as two wild type strains of opposite mating types, were characterized for sclerotium and apothecium development. No aberrant morphology was observed in sclerotium development when single deletion mutants in hydrophobin genes were analyzed. Sclerotia of double knock out mutant ΔBhp1/ΔBhp3 and the triple knock out mutant, however, showed easily wettable phenotypes. For analyzing apothecium development, a reciprocal crossing scheme was setup. Morphological aberrations were observed in crosses with two hydrophobin mutants. When the double knock out mutant ΔBhp1/ΔBhp2 and the triple knock out mutant were used as the maternal parent (sclerotia), and fertilized with wild type microconidia, the resulting apothecia were swollen, dark brown in color and had a blotched surface. After initially growing upwards toward the light source, the apothecia in many cases collapsed due to loss of structural integrity. Aberrant apothecium development was not observed in the reciprocal cross, when these same mutants were used as the paternal parent (microconidia). These results indicate that the presence of hydrophobins in maternal tissue is important for normal development of apothecia of B. cinerea.
    MeSH terms: Fungal Proteins/genetics*; Fungal Proteins/metabolism; Mutation; Spores, Fungal/physiology; Gene Expression; Botrytis/genetics; Botrytis/physiology*; Mycelium/genetics; Mycelium/physiology
  7. Shojaei TR, Mohd Salleh MA, Tabatabaei M, Ekrami A, Motallebi R, Rahmani-Cherati T, et al.
    Braz J Infect Dis, 2014 Nov-Dec;18(6):600-8.
    PMID: 25181404 DOI: 10.1016/j.bjid.2014.05.015
    Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette-Guerin simultaneously. The sensitivity and specificity of the newly developed biosensor were 94.2% and 86.6%, respectively, while the sensitivity and specificity of polymerase chain reaction and nested polymerase chain reaction were considerably lower, 74.2%, 73.3% and 82.8%, 80%, respectively. The detection limits of the sandwich-form fluorescence resonance energy transfer-based biosensor were far lower (10 fg) than those of the polymerase chain reaction and nested polymerase chain reaction (100 fg). Although the cost of the developed nanobiosensor was slightly higher than those of the polymerase chain reaction-based techniques, its unique advantages in terms of turnaround time, higher sensitivity and specificity, as well as a 10-fold lower detection limit would clearly recommend this test as a more appropriate and cost-effective tool for large scale operations.
    MeSH terms: Gold; Humans; Mycobacterium bovis/isolation & purification*; Mycobacterium tuberculosis/isolation & purification*; Sensitivity and Specificity; Sputum/microbiology*; Tellurium; Tuberculosis, Pulmonary/diagnosis*; Reproducibility of Results; Biosensing Techniques/methods*; Polymerase Chain Reaction; Cadmium Compounds; Fluorescence Resonance Energy Transfer/instrumentation; Fluorescence Resonance Energy Transfer/methods; Metal Nanoparticles
  8. Zaman Huri H, Permalu V, Vethakkan SR
    PLoS One, 2014;9(9):e106505.
    PMID: 25181406 DOI: 10.1371/journal.pone.0106505
    Sliding-scale and basal-bolus insulin regimens are two options available for the treatment of severe or acute hyperglycemia in type 2 diabetes mellitus patients. Although its use is not recommended, sliding-scale insulin therapy is still being used widely. The aims of the study were to compare the glycemic control achieved by using sliding-scale or basal-bolus regimens for the management of severe or acute hyperglycemia in patients with type 2 diabetes and to analyze factors associated with the types of insulin therapy used in the management of severe or acute hyperglycemia. This retrospective study was conducted using the medical records of patients with acute or severe hyperglycemia admitted to a hospital in Malaysia from January 2008 to December 2012. A total of 202 patients and 247 admissions were included. Patients treated with the basal-bolus insulin regimen attained lower fasting blood glucose (10.8 ± 2.3 versus 11.6 ± 3.5 mmol/L; p = 0.028) and mean glucose levels throughout severe/acute hyperglycemia (12.3 ± 1.9 versus 12.8 ± 2.2; p = 0.021) compared with sliding-scale insulin regimens. Diabetic ketoacidosis (p = 0.043), cardiovascular diseases (p = 0.005), acute exacerbation of bronchial asthma (p = 0.010), and the use of corticosteroids (p = 0.037) and loop diuretics (p = 0.016) were significantly associated with the type of insulin regimen used. In conclusion, type 2 diabetes patients with severe and acute hyperglycemia achieved better glycemic control with the basal-bolus regimen than with sliding-scale insulin, and factors associated with the insulin regimen used could be identified.
    MeSH terms: Acute Disease; Adult; Diabetes Mellitus, Type 2/complications*; Drug Administration Schedule; Female; Humans; Hyperglycemia/complications*; Hyperglycemia/drug therapy*; Insulin/administration & dosage*; Insulin/therapeutic use*; Male; Middle Aged; Retrospective Studies; Young Adult
  9. Said NA, Gould CM, Lackovic K, Simpson KJ, Williams ED
    Assay Drug Dev Technol, 2014 Sep;12(7):385-94.
    PMID: 25181411 DOI: 10.1089/adt.2014.593
    Metastasis accounts for the poor prognosis of the majority of solid tumors. The phenotypic transition of nonmotile epithelial tumor cells to migratory and invasive "mesenchymal" cells (epithelial-to-mesenchymal transition [EMT]) enables the transit of cancer cells from the primary tumor to distant sites. There is no single marker of EMT; rather, multiple measures are required to define cell state. Thus, the multiparametric capability of high-content screening is ideally suited for the comprehensive analysis of EMT regulators. The aim of this study was to generate a platform to systematically identify functional modulators of tumor cell plasticity using the bladder cancer cell line TSU-Pr1-B1 as a model system. A platform enabling the quantification of key EMT characteristics, cell morphology and mesenchymal intermediate filament vimentin, was developed using the fluorescent whole-cell-tracking reagent CMFDA and a fluorescent promoter reporter construct, respectively. The functional effect of genome-wide modulation of protein-coding genes and miRNAs coupled with those of a collection of small-molecule kinase inhibitors on EMT was assessed using the Target Activation Bioapplication integrated in the Cellomics ArrayScan platform. Data from each of the three screens were integrated to identify a cohort of targets that were subsequently examined in a validation assay using siRNA duplexes. Identification of established regulators of EMT supports the utility of this screening approach and indicated capacity to identify novel regulators of this plasticity program. Pathway analysis coupled with interrogation of cancer-related expression profile databases and other EMT-related screens provided key evidence to prioritize further experimental investigation into the molecular regulators of EMT in cancer cells.
    MeSH terms: Urinary Bladder Neoplasms/drug therapy; Humans; Cell Line, Tumor; Tissue Array Analysis/methods; Drug Discovery/methods*; High-Throughput Screening Assays/methods*; Epithelial-Mesenchymal Transition/drug effects*; Gene Ontology
  10. Saheb Sharif-Askari F, Syed Sulaiman SA, Saheb Sharif-Askari N, Al Sayed Hussain A, Railey MJ
    PLoS One, 2014;9(9):e106517.
    PMID: 25181525 DOI: 10.1371/journal.pone.0106517
    Anticoagulation therapy is usually required in patients with chronic kidney disease (CKD) for treatment or prevention of thromboembolic diseases. However, this benefit could easily be offset by the risk of bleeding.
    MeSH terms: Adult; Aged; Aged, 80 and over; Anticoagulants/adverse effects*; Female; Hemorrhage/blood; Hemorrhage/chemically induced*; Heparin/adverse effects*; Hospitalization; Humans; Male; Middle Aged; Platelet Count; Prospective Studies; Risk Factors; Thromboembolism/prevention & control; Cohort Studies; Proportional Hazards Models; Enoxaparin/adverse effects*; Renal Insufficiency, Chronic/blood; Renal Insufficiency, Chronic/complications; Renal Insufficiency, Chronic/drug therapy*; Kaplan-Meier Estimate
  11. Rennukka M, Sipaut CS, Amirul AA
    Biotechnol Prog, 2014 Nov-Dec;30(6):1469-79.
    PMID: 25181613 DOI: 10.1002/btpr.1986
    This work aims to shed light in the fabrication of poly(3-hydroxybutyrate-co-44%-4-hydroxybutyrate)[P(3HB-co-44%4HB)]/chitosan-based silver nanocomposite material using different contents of silver nanoparticle (SNP); 1-9 wt%. Two approaches were applied in the fabrication; namely solvent casting and chemical crosslinking via glutaraldehyde (GA). A detailed characterization was conducted in order to yield information regarding the nanocomposite material. X-ray diffraction analysis exhibited the nature of the three components that exist in the nanocomposite films: P(3HB-co-4HB), chitosan, and SNP. In term of mechanical properties, tensile strength, and elongation at break were significantly improved up to 125% and 22%, respectively with the impregnation of the SNP. The melting temperature of the nanocomposite materials was increased whereas their thermal stability was slightly changed. Scanning electron microscopy images revealed that incorporation of 9 wt% of SNP caused agglomeration but the surface roughness of the material was significantly improved with the loading. Staphylococcus aureus and Escherichia coli were completely inhibited by the nanocomposite films with 7 and 9 wt% of SNP, respectively. On the other hand, degradation of the nanocomposite materials outweighed the degradation of the pure copolymer. These bioactive and biodegradable materials stand a good chance to serve the vast need of biomedical applications namely management and care of wound as wound dressing.
    MeSH terms: Anti-Infective Agents/metabolism*; Anti-Infective Agents/pharmacology; Anti-Infective Agents/chemistry; Bacteria/drug effects; Hydroxybutyrates/metabolism*; Hydroxybutyrates/pharmacology; Hydroxybutyrates/chemistry; Polyesters/metabolism*; Polyesters/pharmacology; Polyesters/chemistry; Silver/metabolism*; Silver/pharmacology; Silver/chemistry; Surface Properties; X-Ray Diffraction; Chitosan/metabolism*; Chitosan/pharmacology; Chitosan/chemistry; Nanocomposites/chemistry*
  12. Molanorouzi K, Khoo S, Morris T
    BMC Public Health, 2014;14:909.
    PMID: 25182130 DOI: 10.1186/1471-2458-14-909
    Although there is abundant evidence to recommend a physically active lifestyle, adult physical activity (PA) levels have declined over the past two decades. In order to understand why this happens, numerous studies have been conducted to uncover the reasons for people's participation in PA. Often, the measures used were not broad enough to reflect all the reasons for participation in PA. The Physical Activity and Leisure Motivation Scale (PALMS) was created to be a comprehensive tool measuring motives for participating in PA. This 40-item scale related to participation in sport and PA is designed for adolescents and adults. Five items constitute each of the eight sub-scales (mastery, enjoyment, psychological condition, physical condition, appearance, other's expectations, affiliation, competition/ego) reflecting motives for participation in PA that can be categorized as features of intrinsic and extrinsic motivation based on self-determination theory. The aim of the current study was to validate the PALMS in the cultural context of Malaysia, including to assess how well the PALMS captures the same information as the Recreational Exercise Motivation Measure (REMM).
    MeSH terms: Adolescent; Adult; Aged; Female; Humans; Leisure Activities/psychology*; Malaysia; Male; Middle Aged; Motivation*; Patient Participation/statistics & numerical data; Surveys and Questionnaires/standards*; Social Behavior; Sports/psychology*; Sports/statistics & numerical data; Reproducibility of Results; Models, Statistical; Exercise/psychology*; Young Adult
  13. Srijaya TC, Ramasamy TS, Kasim NH
    J Transl Med, 2014;12:243.
    PMID: 25182194 DOI: 10.1186/s12967-014-0243-9
    The inadequacy of existing therapeutic tools together with the paucity of organ donors have always led medical researchers to innovate the current treatment methods or to discover new ways to cure disease. Emergence of cell-based therapies has provided a new framework through which it has given the human world a new hope. Though relatively a new concept, the pace of advancement clearly reveals the significant role that stem cells will ultimately play in the near future. However, there are numerous uncertainties that are prevailing against the present setting of clinical trials related to stem cells: like the best route of cell administration, appropriate dosage, duration and several other applications. A better knowledge of these factors can substantially improve the effectiveness of disease cure or organ repair using this latest therapeutic tool. From a certain perspective, it could be argued that by considering certain proven clinical concepts and experience from synthetic drug system, we could improve the overall efficacy of cell-based therapies. In the past, studies on synthetic drug therapies and their clinical trials have shown that all the aforementioned factors have critical ascendancy over its therapeutic outcomes. Therefore, based on the knowledge gained from synthetic drug delivery systems, we hypothesize that by employing many of the clinical approaches from synthetic drug therapies to this new regenerative therapeutic tool, the efficacy of stem cell-based therapies can also be improved.
    MeSH terms: Drug Therapy/methods; Drug Therapy/trends*; Humans; Specimen Handling/methods; Stem Cells/cytology; Stem Cells/physiology; Transplantation, Homologous; Treatment Outcome; Point-of-Care Systems/trends*; Stem Cell Transplantation/methods; Stem Cell Transplantation/trends*
  14. Wong MH, Lim LF, Ahmad FB, Assim ZB
    Asian Pac J Trop Biomed, 2014 May;4(5):386-92.
    PMID: 25182724 DOI: 10.12980/APJTB.4.2014C1129
    To investigate antioxidant and antimicrobial activities of two plant species, Litsea elliptica (L. elliptica) and Litsea resinosa (L. resinosa).
  15. Jose S, Tan SW, Ooi YY, Ramasamy R, Vidyadaran S
    J Neuroinflammation, 2014;11:149.
    PMID: 25182840 DOI: 10.1186/s12974-014-0149-8
    Progression of neurodegenerative diseases occurs when microglia, upon persistent activation, perpetuate a cycle of damage in the central nervous system. Use of mesenchymal stem cells (MSC) has been suggested as an approach to manage microglia activation based on their immunomodulatory functions. In the present study, we describe the mechanism through which bone marrow-derived MSC modulate the proliferative responses of lipopolysaccharide-stimulated BV2 microglia.
    MeSH terms: Animals; Cell Cycle/drug effects; Cell Cycle/physiology; Cell Line, Transformed; Enzyme Inhibitors/pharmacology; Lipopolysaccharides/pharmacology; Nitric Oxide/metabolism; Thymidine/metabolism; Time Factors; Tritium/metabolism; Tumor Necrosis Factor-alpha/metabolism*; Antigens, CD/metabolism; Cytokines/metabolism*; Apoptosis; Microglia/drug effects; Microglia/metabolism*; Coculture Techniques; NG-Nitroarginine Methyl Ester/pharmacology; Protein Array Analysis; Cell Proliferation/drug effects; Cell Proliferation/physiology*; Mice; Mesenchymal Stromal Cells/physiology*
  16. Vikram P, Chiruvella KK, Ripain IH, Arifullah M
    Asian Pac J Trop Biomed, 2014 Jun;4(6):430-5.
    PMID: 25182942 DOI: 10.12980/APJTB.4.2014C1255
    Medicinal plants and herbal preparations are gaining renowned interest in scientific communities nowadays due to their reliable pharmacological actions and affordability to common people which makes them effective in control of various diseases. Polygonum minus (Polygonaceae) locally known as kesum is an aromatic plant commonly used in Malay delicacies. The plant is having potential applications due to its high volatile oil constituents in perfumes and powerful antioxidant activity. It has been used traditionally to treat various ailments including dandruff. The research has been carried out by various researchers using different in vitro and in vivo models for biological evaluations to support these claims. This review paper may help upcoming research activities on Polygonum minus by giving up to date information on the phytochemical constituents and medicinal properties of kesum to a possible extent with relevant data.
  17. Huat LB, Garcia AO, Ning TZ, Kin WW, Noordin R, Azham SS, et al.
    Asian Pac J Trop Biomed, 2014 Jun;4(6):446-50.
    PMID: 25182945 DOI: 10.12980/APJTB.4.2014C1169
    To characterize the Entamoeba histolytica (E. histolytica) antigen(s) recognized by moribound amoebic liver abscess hamsters.
  18. Kumar A
    Asian Pac J Trop Biomed, 2014 May;4(Suppl 1):S189-97.
    PMID: 25183079 DOI: 10.12980/APJTB.4.2014D153
    OBJECTIVE: To address the association of dietary vitamins, anthropometric profile, lipid profile, antioxidant enzymes and lipid peroxidation in hypertensive participant compared with normotensive healthy controls.

    METHODS: Dietary intake of vitamins was assessed by 131 food frequency questionnaire items in both hypertensive participants and normotensive age-sex matched healthy controls. The associated changes in serum antioxidants and lipid peroxidation were also assessed along with lipid profile and anthropometric measurements in both groups of subjects under study.

    RESULTS: Dietary vitamins intake was higher in hypertensive participants excepting for vitamin B2 and ascorbic acid compared to normotensive controls. Anthropometric variables in the hypertensive showed significant differences in weight, body mass index, waist circumference, hip circumference, waist-hip ratio and mid-arm circumference. The total cholesterol, low-density lipoprotein cholesterol, triglyceride were significantly higher (P<0.001) in hypertensive except high-density lipoprotein cholesterol which was significantly higher (P<0.001) in normotensive. The serum endogenous antioxidants and enzyme antioxidants were significantly decreased in hypertensive except serum albumin levels compared to normotensive along with concomitant increase in serum lipoprotein (a) malondialdehyde and conjugated diene levels.

    CONCLUSIONS: Based on the observations, our study concludes that hypertension is caused due to interplay of several confounding factors namely anthropometry, lipid profile, depletion of endogenous antioxidants and rise in oxidative stress.

  19. Bhagavathula AS, Berhanie A, Tigistu H, Abraham Y, Getachew Y, Khan TM, et al.
    Asian Pac J Trop Biomed, 2014 May;4(Suppl 1):S204-8.
    PMID: 25183081 DOI: 10.12980/APJTB.4.2014C1172
    To determine the prevalence, clinical significance and the associated risk factors of potential drug-drug interactions (DDIs) at internal medicine ward of University of Gondar (UOG) hospital.
  20. Azmi N, Hashim P, Hashim DM, Halimoon N, Majid NM
    Asian Pac J Trop Biomed, 2014 May;4(Suppl 1):S348-52.
    PMID: 25183109 DOI: 10.12980/APJTB.4.2014C1166
    To examine whether earthworms of Eisenia fetida, Lumbricus rubellus and Eudrilus eugeniae extracts have elastase, tyrosinase and matrix metalloproteinase-1 (MMP-1) inhibitory activity.
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