Browse publications by year: 2019

  1. Ganggayah MD, Taib NA, Har YC, Lio P, Dhillon SK
    BMC Med Inform Decis Mak, 2019 03 22;19(1):48.
    PMID: 30902088 DOI: 10.1186/s12911-019-0801-4
    BACKGROUND: Breast cancer is one of the most common diseases in women worldwide. Many studies have been conducted to predict the survival indicators, however most of these analyses were predominantly performed using basic statistical methods. As an alternative, this study used machine learning techniques to build models for detecting and visualising significant prognostic indicators of breast cancer survival rate.

    METHODS: A large hospital-based breast cancer dataset retrieved from the University Malaya Medical Centre, Kuala Lumpur, Malaysia (n = 8066) with diagnosis information between 1993 and 2016 was used in this study. The dataset contained 23 predictor variables and one dependent variable, which referred to the survival status of the patients (alive or dead). In determining the significant prognostic factors of breast cancer survival rate, prediction models were built using decision tree, random forest, neural networks, extreme boost, logistic regression, and support vector machine. Next, the dataset was clustered based on the receptor status of breast cancer patients identified via immunohistochemistry to perform advanced modelling using random forest. Subsequently, the important variables were ranked via variable selection methods in random forest. Finally, decision trees were built and validation was performed using survival analysis.

    RESULTS: In terms of both model accuracy and calibration measure, all algorithms produced close outcomes, with the lowest obtained from decision tree (accuracy = 79.8%) and the highest from random forest (accuracy = 82.7%). The important variables identified in this study were cancer stage classification, tumour size, number of total axillary lymph nodes removed, number of positive lymph nodes, types of primary treatment, and methods of diagnosis.

    CONCLUSION: Interestingly the various machine learning algorithms used in this study yielded close accuracy hence these methods could be used as alternative predictive tools in the breast cancer survival studies, particularly in the Asian region. The important prognostic factors influencing survival rate of breast cancer identified in this study, which were validated by survival curves, are useful and could be translated into decision support tools in the medical domain.

    MeSH terms: Adult; Breast Neoplasms/diagnosis*; Breast Neoplasms/mortality*; Decision Trees*; Female; Humans; Middle Aged; Prognosis; Neural Networks (Computer)*; Support Vector Machine*
  2. Thangavelu SK, Rajkumar T, Pandi DK, Ahmed AS, Ani FN
    Waste Manag, 2019 Mar 01;86:80-86.
    PMID: 30902242 DOI: 10.1016/j.wasman.2019.01.035
    Microwave assisted acid hydrolysis (H2SO4 and HCl with >0.5 mol/L) to produce bioethanol from sago pith waste (SPW) was studied. The energy consumption for microwave hydrolysis at different energy inputs and acid concentration were calculated. The overall energy consumption for bioethanol fuel production from SPW was assessed. A maximum of 88% glucose yield and 80% ethanol yield (3.1 g ethanol per 10 g SPW) were obtained using 1.0 mol/L H2SO4. Microwave hydrolysis using 1.0 mol/L H2SO4 consumed the minimum energy of 8.1 kJ to produce 1 g glucose from SPW when energy input was fixed at 54 kJ (900 W for 1 min). In general, 1 g glucose can produce 16 kJ. The overall energy consumption for fuel grade bioethanol production from SPW was 31.77 kJ per g ethanol, which was slightly higher than the lower heating values of ethanol (26.74 kJ/g ethanol).
    MeSH terms: Ethanol*; Fermentation; Glucose; Hydrolysis; Microwaves*; Biomass; Biofuels
  3. Lorenzo-Morales J, Sifaoui I, Reyes-Batlle M, Lares-Villa F, Lares-Jiménez LF, Omaña-Molina M, et al.
    Exp Parasitol, 2019 May;200:36.
    PMID: 30902622 DOI: 10.1016/j.exppara.2019.03.010
    MeSH terms: Amebiasis/parasitology*; Amoeba/pathogenicity*; Amoeba/physiology*; Animals; Humans; Opportunistic Infections/parasitology*
  4. Al-Qubaisi MS, Rasedee A, Flaifel MH, Eid EEM, Hussein-Al-Ali S, Alhassan FH, et al.
    Eur J Pharm Sci, 2019 May 15;133:167-182.
    PMID: 30902654 DOI: 10.1016/j.ejps.2019.03.015
    Thymoquinone is an effective phytochemical compound in the treatment of various diseases. However, its practical administration has been limited due to poor aqueous solubility and bioavailability. In this work, we developed a novel inclusion complex of thymoquinone and hydroxypropyl-β-cyclodextrin that features improved solubility and bioactivity. The drug solubility was markedly accelerated in the increasing ratio of hydroxypropyl-β-cyclodextrin to thymoquinone amount. The formation of the thymoquinone/hydroxypropyl-β-cyclodextrin inclusion complex was evidenced using X-ray diffraction, differential scanning calorimetry, thermal gravimetric analysis, Fourier transform infrared, scanning electron microscopy and nuclear magnetic resonance. The release behavior of the complex, as well as of their mixtures, was examined in artificial gastric (pH 1.2) and intestinal (pH 6.8) dissolution media. The formulated complex released the drug rapidly at the initial stage, followed by a slow release. Thermodynamic parameters ΔH, ΔS and ΔG were calculated with temperatures ranging from 20 to 45 °C to evaluate the complexation process. The activity of the inclusion complex was evaluated on IgE-mediated allergic response in rat basophilic leukemia (RBL-2H3) cells by monitoring key allergic mediators. The results revealed that compared with free thymoquinone, the inclusion complex more strongly inhibited the release of histamine, tumor necrosis factor-α, and interleukin-4, and was not cytotoxic at the tested thymoquinone concentrations (0.125-4 μg/mL) indicating the inclusion complex possibly had better antiallergic effects. Our finding suggested that the inclusion complex achieved prolonged action and reduced side-effect of thymoquinone.
    MeSH terms: Animals; Gastric Juice/chemistry; Histamine/metabolism; Intestinal Secretions/chemistry; Tumor Necrosis Factor-alpha/metabolism; Interleukin-4/metabolism; Benzoquinones/administration & dosage*; Benzoquinones/chemistry; Drug Delivery Systems*; Anti-Allergic Agents/administration & dosage*; Anti-Allergic Agents/chemistry; Cell Line, Tumor; Rats; Drug Liberation
  5. Fuchs J, Bessire K, Weiler S
    Toxicon, 2019 Mar 20;163:44-47.
    PMID: 30902684 DOI: 10.1016/j.toxicon.2019.03.019
    This case report describes the effect of an envenomation by the Beautiful Pit Viper Trimeresurus venustus (or Cryptelytrops venustus), which is a green pit viper native to Thailand and Malaysia. A previously healthy 60-year-old snake breeder with no relevant medical history was bitten by his adult T. venustus in the third finger of his right hand while taking it out of the feeding box. The bite was painful and swelling progressed to include his whole hand within an hour after the bite. He was treated symptomatically with pre-emptive antibiotics and analgesics, never developed any hematological disorders such as coagulopathy and bleeding or disseminated intravascular coagulation and was discharged 26 hours after the bite. The clinical course in our patient matched two other well-documented cases reported to our Poisons Centre, and one further case presented as a conference-poster. All patients recovered with symptomatic therapy and never required antivenom. Therefore, bites by T. venustus seem to present with less severe symptoms compared to other Trimeresurus species.
    MeSH terms: Adult; Analgesics; Animals; Anti-Bacterial Agents; Antivenins; Blood Coagulation Disorders; Disseminated Intravascular Coagulation; Humans; Malaysia; Male; Pain; Poisons; Snakes; Thailand; Trimeresurus
  6. Kwan TJM, Zilany MSA, Davies-Venn E, Abdul Wahab AK
    Exp Brain Res, 2019 Jun;237(6):1479-1491.
    PMID: 30903206 DOI: 10.1007/s00221-019-05511-4
    Various studies on medial olivocochlear (MOC) efferents have implicated it in multiple roles in the auditory system (e.g., dynamic range adaptation, masking reduction, and selective attention). This study presents a systematic simulation of inferior colliculus (IC) responses with and without electrical stimulation of the MOC. Phenomenological models of the responses of auditory nerve (AN) fibers and IC neurons were used to this end. The simulated responses were highly consistent with physiological data (replicated 3 of the 4 known rate-level responses all MOC effects-shifts, high stimulus level reduction and enhancement). Complex MOC efferent effects which were previously thought to require integration from different characteristic frequency (CF) neurons were simulated using the same frequency inhibition excitation circuitry. MOC-induced enhancing effects were found only in neurons with a CF range from 750 Hz to 2 kHz. This limited effect is indicative of the role of MOC activation on the AN responses at the stimulus offset.
    MeSH terms: Cochlea/physiology*; Cochlear Nerve/physiology*; Efferent Pathways/physiology*; Electric Stimulation; Humans; Inferior Colliculi/physiology*; Models, Theoretical*; Superior Olivary Complex/physiology*
  7. Makker K, Lamba AK, Faraz F, Tandon S, Sheikh Ab Hamid S, Aggarwal K, et al.
    Cell Tissue Bank, 2019 Jun;20(2):243-253.
    PMID: 30903410 DOI: 10.1007/s10561-019-09763-w
    During bone allograft processing, despite stringent donor screening and use of aseptic techniques, microbial invasion may occur due to the porous nature of the graft and cause potentially fatal infections. The aim of the present study was to prepare bone allograft with and without gentamicin and to compare bioburden and sterility in the obtained grafts to evaluate the role of antibiotic in enhancing graft safety. Fifty samples of demineralized freeze-dried bone allograft were prepared from suitable donors according to international standards. Randomly selected 25 samples were placed in 8 mg gentamicin/gram bone solution for 1 h. Packaging and sealing was done to ensure no microbial ingress during transportation. 40 samples were selected for bioburden testing. Remaining 10 were subjected to 25 kGy gamma radiation and tested for sterility. Microbiological evaluation revealed no evidence of colony forming units in all the samples of both the groups (Bioburden = 0). Post-radiation sterility testing also revealed no bacterial colony in the tested samples from both the groups. Favorable results validate the processing protocol while comparable results in both groups indicate no additive benefit of gentamicin addition. Nil bioburden may be used in further studies to determine a lower radiation dose to achieve adequate sterility and minimize the disadvantages of radiation like collagen cross-linking and decreased osteoinductive capacity.
    MeSH terms: Anti-Bacterial Agents/therapeutic use*; Gamma Rays/therapeutic use*; Gentamicins/therapeutic use*; Humans; Sterilization/methods*; Surgical Wound Infection/prevention & control*; Transplantation, Homologous/adverse effects; Bone Transplantation/adverse effects*; Arthroplasty, Replacement, Hip; Bacterial Load/drug effects
  8. Abdul Rahim MBH, Chilloux J, Martinez-Gili L, Neves AL, Myridakis A, Gooderham N, et al.
    Acta Diabetol, 2019 May;56(5):493-500.
    PMID: 30903435 DOI: 10.1007/s00592-019-01312-x
    The human gut is a home for more than 100 trillion bacteria, far more than all other microbial populations resident on the body's surface. The human gut microbiome is considered as a microbial organ symbiotically operating within the host. It is a collection of different cell lineages that are capable of communicating with each other and the host and has an ability to undergo self-replication for its repair and maintenance. As the gut microbiota is involved in many host processes including growth and development, an imbalance in its ecological composition may lead to disease and dysfunction in the human. Gut microbial degradation of nutrients produces bioactive metabolites that bind target receptors, activating signalling cascades, and modulating host metabolism. This review covers current findings on the nutritional and pharmacological roles of selective gut microbial metabolites, short-chain fatty acids, methylamines and indoles, as well as discussing nutritional interventions to modulate the microbiome.
    MeSH terms: Gastrointestinal Microbiome/drug effects; Gastrointestinal Microbiome/physiology*; Bacteria; Diet*; Fatty Acids, Volatile/administration & dosage*; Humans; Indoles/administration & dosage*; Methylamines/administration & dosage*; Microbiota/drug effects; Microbiota/physiology
  9. Uddin GA, Alam K, Gow J
    Environ Sci Pollut Res Int, 2019 May;26(13):13159-13172.
    PMID: 30903468 DOI: 10.1007/s11356-019-04791-1
    The relationship between national income growth and the environment of 14 Asian economies over a 50 year period is examined using the Environmental Kuznets Curve (EKC) hypothesis. Ecological Footprint (EF) measures environmental impacts and gross domestic product (GDP) measures economic growth. It is hypothesised that increased rates of economic growth come at a cost to the natural environment. The EKC hypothesis has been mainly tested in the literature by cross-sectional or panel data methods. In this study, it is tested using time series analysis through initially examining the relationship between EF and GDP using linear, quadratic and cubic estimating OLS regression functions. In the second stage, the long-run relationship between EF and GDP is investigated using an augmented error correction trend model. There is a statistically significant cointegrated long-run relationship between the variables in most of the countries. The EKC hypothesis is supported in the case of India, Nepal, Malaysia and Pakistan with the other countries exhibiting a positive linear relationship between the two variables. Almost all error correction terms are correct in sign and significance that implies that some percentage of disequilibria in EF in the previous year adjusts back to the long-run equilibrium in the current year. Based on the long-run relationship, it is apparent that rapid economic growth has had an impact on the environment and the ecosystems of these countries over the last 50 years. Despite that, until now, not many of them have taken sufficient steps to reduce their EF or to improve their bioproductive capacity.
    MeSH terms: Carbon Dioxide/analysis; Cross-Sectional Studies; Ecology*; Environment; Environmental Monitoring; Income; India; Malaysia; Nepal; Pakistan; Ecosystem; Economic Development*; Gross Domestic Product
  10. Lee SWH
    Curr Pharm Teach Learn, 2019 03;11(3):292-295.
    PMID: 30904152 DOI: 10.1016/j.cptl.2018.12.010
    BACKGROUND AND PURPOSE: To describe the development and assessment of a health education campaign and determine its potential for integration into a bachelor of pharmacy curriculum.

    EDUCATIONAL ACTIVITY AND SETTING: Pharmacy students developed a "hands-on" health campaign for delivery to university students. A health promotion topic was chosen and delivered each year for 2015-2017; sexual health, diabetes, and antimicrobial resistance, respectively. All health campaign participants were screened for cardiovascular risk factors. University students who participated in the health campaign in 2017 also completed a questionnaire assessing their understanding and knowledge of antimicrobial resistance.

    FINDINGS: In the three health campaigns conducted from 2015 to 2017, 1010 university students and adults were screened. Pharmacy students expressed a high level of professional achievement and satisfaction and felt that the activities provided a meaningful learning experience. Similarly, supervising pharmacists reported satisfaction with students' competency.

    SUMMARY: The use of a health education campaign is an alternative model to educate pharmacy students on communication and critical thinking skills, as well as provide an opportunity for service learning.

    MeSH terms: Health Education/methods*; Health Education/standards; Health Education/statistics & numerical data; Health Promotion/methods; Humans; Malaysia; Referral and Consultation; Students, Pharmacy/statistics & numerical data*; Thinking; Universities/organization & administration; Universities/statistics & numerical data; Program Development/methods
  11. Reid MJA, Arinaminpathy N, Bloom A, Bloom BR, Boehme C, Chaisson R, et al.
    Lancet, 2019 Mar 30;393(10178):1331-1384.
    PMID: 30904263 DOI: 10.1016/S0140-6736(19)30024-8
    MeSH terms: Goals; Health Policy; Health Priorities; Humans; Leadership; Mortality; Mycobacterium tuberculosis/drug effects; Mycobacterium tuberculosis/pathogenicity; Political Systems; Quality of Health Care/standards; Quality of Health Care/trends*; Research/economics*; Tuberculosis, Pulmonary/drug therapy; Tuberculosis, Pulmonary/economics*; Tuberculosis, Pulmonary/epidemiology; Tuberculosis, Pulmonary/prevention & control*; Global Health/legislation & jurisprudence*; Global Health/statistics & numerical data; World Health Organization/economics; Incidence; Cost of Illness; Disease Eradication
  12. Habiba U, Lee JJL, Joo TC, Ang BC, Afifi AM
    Int J Biol Macromol, 2019 Jun 15;131:821-827.
    PMID: 30904531 DOI: 10.1016/j.ijbiomac.2019.03.132
    In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV. Field-emission scanning electron microscopic images showed bead free finer nanofiber. Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2. X-ray powder diffraction showed that TiO2 became amorphous in the composite nanofiber. Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2. The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium. But degraded in acidic and water medium after 93 and 162 h, respectively. The adsorption mechanism of congo red obeyed the Langmuir isotherm model. On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models. The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively. However, a high dose of adsorbent was required for congo red.
    MeSH terms: Adsorption; Azo Compounds/chemistry*; Congo Red/chemistry*; Membranes, Artificial; Polyvinyl Alcohol/chemistry*; Spectrum Analysis; Tensile Strength; Thermodynamics; Titanium/chemistry*; Water Pollutants, Chemical/chemistry; Water Purification; Chitosan/chemistry*; Nanofibers/ultrastructure; Nanofibers/chemistry*
  13. Chan SY, Goh CF, Lau JY, Tiew YC, Balakrishnan T
    Int J Pharm, 2019 May 01;562:203-211.
    PMID: 30904726 DOI: 10.1016/j.ijpharm.2019.03.044
    Rice starch is known to have an excellent film-forming behaviour in the packaging industry but inadequate attention was given to this biopolymer to be developed into thin films for drug delivery. Accordingly, rice starch thin films containing a model drug, paracetamol and plasticisers (glycerol or sorbitol) were developed using film casting technique. This study focuses on investigating the impact of plasticiser and drug loading on drug release pattern of rice starch films which has not been explored to date. The obtained rice films were characterised for their physicochemical properties including swelling and dissolution study. The highest drug dissolution rate was achieved in the rice films with a low drug loading due to drug amorphicity in nature. When drug loading increases, the swelling behaviour of rice films plays a dominant role in releasing drug in the crystalline form. The role of plasticiser was indicated by the plasticiser-starch interaction where a strong interaction allows drug solubilisation more readily in the dissolution medium. It is envisaged that rice films could be tailored to achieve desired drug release pattern with different plasticiser.
    MeSH terms: Acetaminophen/chemistry; Administration, Buccal; Plasticizers/chemistry; Oryza*; Starch*; Drug Delivery Systems*; Drug Liberation
  14. Xin GLL, Khee YP, Ying TY, Chellian J, Gupta G, Kunnath AP, et al.
    Curr. Diab. Rep., 2019 03 23;19(5):22.
    PMID: 30905013 DOI: 10.1007/s11892-019-1144-3
    PURPOSE OF REVIEW: Type 1 diabetes (T1D) occurs when there is destruction of beta cells within the islets of Langerhans in the pancreas due to autoimmunity. It is considered a complex disease, and different complications can surface and worsen the condition if T1D is not managed well. Since it is an incurable disease, numerous treatments and therapies have been postulated in order to control T1D by balancing hyperglycemia control while minimizing hypoglycemic episodes. The purpose of this review is to primarily look into the current state of the available immunological therapies and their advantages for the treatment of T1D.

    RECENT FINDINGS: Over the years, immunological therapy has become the center of attraction to treat T1D. Immunomodulatory approaches on non-antigens involving agents such as cyclosporine A, mycophenolate mofetil, anti-CD20, cytotoxic T cells, anti-TNF, anti-CD3, and anti-thymocyte globulin as well as immunomodulative approaches on antigens such as insulin, glutamic acid decarboxylase, and heat shock protein 60 have been studied. Aside from these two approaches, studies and trials have also been conducted on regulatory T cells, dendritic cells, interleukin 2, interleukin 4, M2 macrophages, and rapamycin/interleukin 2 combination therapy to test their effects on patients with T1D. Many of these agents have successfully suppressed T1D in non-obese diabetic (NOD) mice and in human trials. However, some have shown negative results. To date, the insights into the management of the immune system have been increasing rapidly to search for potential therapies and treatments for T1D. Nevertheless, some of the challenges are still inevitable. A lot of work and effort need to be put into the investigation on T1D through immunological therapy, particularly to reduce complications to improve and enhance clinical outcomes.

    MeSH terms: Animals; Diabetes Mellitus, Type 1*; Humans; Immunotherapy; Insulin; Tumor Necrosis Factor-alpha; Mice, Inbred NOD
  15. Elhusseiny KM, Abd-Elhay FA, Kamel MG, Abd El Hamid Hassan HH, El Tanany HHM, Hieu TH, et al.
    Head Neck, 2019 08;41(8):2625-2635.
    PMID: 30905082 DOI: 10.1002/hed.25742
    BACKGROUND: We aimed to investigate the prognostic role of examined (dissected) lymph nodes (ELNs), negative LNs (NLNs), and positive (metastatic) LNs (PLNs) counts and LN ratio (LNR = PLNs/ELNs×100) in patients with major salivary gland cancer (SGC).

    METHODS: Data were retrieved for major SGC patients diagnosed between 1988 and 2011 from Surveillance, Epidemiology, and End Results program.

    RESULTS: We have included 5446 patients with major SGC. Most patients had parotid gland cancer (84.61%). Patients having >18 ELNs, >4 PLNs, and >33.33% LNR were associated with a worse survival. Moreover, older age, male patients, grade IV, distant stage, unmarried patients, submandibular gland cancer, and received chemotherapy but not received surgery were significantly associated with a worse survival.

    CONCLUSIONS: We demonstrated that patients with >18 ELNs and >4 PLNs counts, and >33.33% LNR were high-risk group patients. We strongly suggest adding the ELNs and PLNs counts and/or LNR into the current staging system.

    MeSH terms: Adult; Female; Humans; Lymph Nodes/pathology*; Lymphatic Metastasis*; Male; Middle Aged; Neoplasm Staging/methods*; Prognosis; Salivary Gland Neoplasms/mortality; Salivary Gland Neoplasms/pathology*; Proportional Hazards Models; Kaplan-Meier Estimate
  16. Zulfarina MS, Syarifah-Noratiqah SB, Nazrun SA, Sharif R, Naina-Mohamed I
    Clin Psychopharmacol Neurosci, 2019 May 31;17(2):145-154.
    PMID: 30905115 DOI: 10.9758/cpn.2019.17.2.145
    Panic disorder (PD) being one of the most intensively investigated anxiety disorders is considered a heterogeneous psychiatric disease which has difficulties with early diagnosis. The disorder is recurrent and usually associated with low remission rates and high rates of relapse which may exacerbated social and quality of life, causes unnecessary cost and increased risk for complication and suicide. Current pharmacotherapy for PD are available but these drugs have slow therapeutic onset, several side effects and most patients do not fully respond to these standard pharmacological treatments. Ongoing investigations indicate the need for new and promising agents for the treatment of PD. This article will cover the importance of immediate and proper treatment, the gap in the current management of PD with special emphasis on pharmacotherapy, and evidence regarding the novel anti-panic drugs including the drugs in developments such as metabotropic glutamate (mGlu 2/3) agonist and levetiracetam. Preliminary results suggest the anti-panic properties and the efficacy of duloxetine, reboxetine, mirtazapine, nefazodone, risperidone and inositol as a monotherapy drug. Apart for their effectiveness, the aforementioned compounds were generally well tolerated compared to the standard available pharmacotherapy drugs, indicating their potential therapeutic usefulness for ambivalent and hypervigilance patient. Further strong clinical trials will provide an ample support to these novel compounds as an alternative monotherapy for PD treatment-resistant patient.
    MeSH terms: Duloxetine Hydrochloride; Anxiety; Humans; Inositol; Mianserin; Morpholines; Panic; Piracetam; Quality of Life; Recurrence; Suicide; Triazoles; Panic Disorder; Glutamic Acid; Risperidone; Early Diagnosis
  17. Mohd-Nor D, Ramli N, Sharuddin SS, Hassan MA, Mustapha NA, Ariffin H, et al.
    Microbes Environ, 2019 Jun 27;34(2):121-128.
    PMID: 30905894 DOI: 10.1264/jsme2.ME18104
    Despite efforts to address the composition of the microbial community during the anaerobic treatment of palm oil mill effluent (POME), its composition in relation to biodegradation in the full-scale treatment system has not yet been extensively examined. Therefore, a thorough analysis of bacterial and archaeal communities was performed in the present study using MiSeq sequencing at the different stages of the POME treatment, which comprised anaerobic as well as facultative anaerobic and aerobic processes, including the mixed raw effluent (MRE), mixing pond, holding tank, and final discharge phases. Based on the results obtained, the following biodegradation processes were suggested to occur at the different treatment stages: (1) Lactobacillaceae (35.9%) dominated the first stage, which contributed to high lactic acid production; (2) the higher population of Clostridiaceae in the mixing pond (47.7%) and Prevotellaceae in the holding tank (49.7%) promoted acetic acid production; (3) the aceticlastic methanogen Methanosaetaceae (0.6-0.8%) played a role in acetic acid degradation in the open digester and closed reactor for methane generation; (4) Syntrophomonas (21.5-29.2%) appeared to be involved in the degradation of fatty acids and acetic acid by syntrophic cooperation with the hydrogenotrophic methanogen, Methanobacteriaceae (0.6-1.3%); and (5) the phenols and alcohols detected in the early phases, but not in the final discharge phase, indicated the successful degradation of lignocellulosic materials. The present results contribute to a better understanding of the biodegradation mechanisms involved in the different stages of the full-scale treatment of POME.
    MeSH terms: Aerobiosis; Anaerobiosis; Archaea/classification; Archaea/isolation & purification; Archaea/metabolism; Bacteria/classification; Bacteria/isolation & purification; Bacteria/metabolism; Biodegradation, Environmental*; Lignin/metabolism; Methane/biosynthesis; Sewage/microbiology*; Waste Disposal, Fluid*; Bioreactors/microbiology; Biodiversity; Microbial Consortia*
  18. Arya SS, Kaimal AM, Chib M, Sonawane SK, Show PL
    J Food Sci Technol, 2019 Feb;56(2):524-534.
    PMID: 30906010 DOI: 10.1007/s13197-018-3546-7
    Recently, a novel technique for extraction of functional thermally sensitive bioactive components from food has been developed due to its green efficacy (no toxic chemicals) and cost effectiveness. Cloud point extraction (CPE) is one of the such best alternative techniques that can be used for extraction of wide range of organic and inorganic components using green surfactants. It is a simple, rapid and inexpensive extraction technique which involves clustering of non-ionic surfactant monomers to form a hydrophobic core (micelle), which then entraps the hydrophobic bioactive compounds within it. CPE can be applied for extraction of bioactives from food processing waste as well as separation and purification of proteins. Besides that, research has received special attention on sample preparation for analysis of food constituents in the last decade. The scope of CPE is very vast in these sectors because of the advantages of CPE over other methods. This review deals with significance of CPE method and their potential green applications in food processing.
    MeSH terms: Attention; Cost-Benefit Analysis; Food Handling; Micelles; Research; Surface-Active Agents; Cluster Analysis; Hydrophobic and Hydrophilic Interactions
  19. Alara OR, Abdurahman NH
    J Food Sci Technol, 2019 Feb;56(2):580-588.
    PMID: 30906015 DOI: 10.1007/s13197-018-3512-4
    Recently, unconventional methods especially microwave-assisted hydrodistillation extraction (MAHE) is being used as an alternative technique for extracting bioactive compounds from plant materials due to its advantages over conventional methods such as Soxhlet extraction (SE). In this study, bioactive compounds were extracted from Vernonia cinerea leaf using both MAHE and SE methods. In addition, the kinetic study of MAHE and SE methods were carried out using first- and second-order kinetic models. The results obtained showed that MAHE can extract higher yield of bioactive compounds from V. cinerea leaf in a shorter time and reduced used of extracting solvent compared with SE method. Based on the results obtained, second-order kinetic models can actually describe the extraction of bioactive compounds from V. cinerea leaf through MAHE with extraction rate coefficient of 0.1172 L/gmin and extraction capacity of 1.0547 L/g as compared to SE with 0.0157 L/gmin and 1.1626 L/g of extraction rate coefficient and extraction capacity, respectively. The gas chromatography-mass spectrometry analysis of the oil showed the presence of numerous heavy fractions in the oil obtained through MAHE as compared with the SE method. Moreover, the electric consumption and environmental impacts analysis of the oil suggested that MAHE can be a suitable green technique for extracting bioactive compounds from V. cinerea leaf.
    MeSH terms: Electricity; Environment; Gas Chromatography-Mass Spectrometry; Microwaves; Plant Extracts; Solvents; Plant Leaves; Vernonia
  20. Ng ZX, Rosman NF
    J Food Sci Technol, 2019 Feb;56(2):865-877.
    PMID: 30906044 DOI: 10.1007/s13197-018-3547-6
    This study aimed to investigate the effect of in vitro digestion on the antioxidant activity and carbohydrate-digestive enzymes inhibitory potential of five edible mushrooms after subjected to four domestic cooking; namely, boiling, microwaving, steaming and pressure-cooking. The water extracts of raw (uncooked), cooked and in vitro digested mushrooms were compared for their water-soluble phenolic content (WPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), radical scavenging activity (TEAC and DPPH), anti-α-amylase and anti-α-glucosidase activities. Among the raw samples, Lentinula edodes possessed the highest antioxidant activities (FRAP, TEAC, DPPH) and WPC while Pleurotus sajor-caju displayed the highest TFC, anti-α-amylase and anti-α-glucosidase activities. The antioxidant and carbohydrate-digestive enzyme inhibitory activities significantly varied according to mushroom species and cooking methods applied. Short duration of microwaving (Agaricus bisporus and Flammulina velutipes), boiling (Auricularia polytricha) and pressure cooking (L. edodes and P. sajor-caju) yielded the best antioxidant and carbohydrate-digestive enzymes inhibition values in the mushroom extracts. TFC was positively correlated with the antioxidant activities and anti-α-glucosidase activity in the mushroom extracts. In vitro digestion significantly improved the total antioxidant and anti-α-glucosidase activities but decreased the anti-α-amylase activity in the cooked mushroom extracts. Principle component analysis showed that in vitro digestion and the cooking process accounted for respective 48.9% and 19.7% of variation in the observed activities. Domestic cooking and in vitro digestion could potentiate the total antioxidant and carbohydrate-digestive enzymes inhibitory activities in the selected water extract of edible mushrooms.
    MeSH terms: Agaricus; alpha-Amylases; Antioxidants; Carbohydrates; Cooking; Flavonoids; Phenols; Steam; Water; Pleurotus; Shiitake Mushrooms; Flammulina
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