Browse publications by year: 2020

  1. Sazlina SG, Sooryanarayana R, Ho BK, Omar MA, Krishnapillai AD, Mohd Tohit N, et al.
    PLoS One, 2020;15(10):e0240826.
    PMID: 33085718 DOI: 10.1371/journal.pone.0240826
    Study on cardiovascular disease (CVD) risk factors and their prevalence among the older people in Malaysia is limited. We aimed to determine the prevalence and factors associated with CVD risk factors using the non-laboratory Framingham Generalized 10-Year CVD risk score among older people in Malaysia. This was a population-based cross-sectional study using data of 3,375 participants aged ≥60 years from the National Health and Morbidity Survey 2015. Sociodemographic, health factors and clinical assessments (anthropometry and blood pressure) were included. Complex survey analysis was used to obtain prevalence with 95% confidence intervals (CI). We applied ordinal regression to determine the factors associated with CVD risk. The prevalence for the high 10-year CVD risk was 72.1%. Body mass index was higher among those aged 60-69 years in men (25.4kg/m2, 95%CI 25.1-25.8) and women (26.7kg/m2, 95%CI 26.3-27.1) than the other age groups. The factors associated with moderate and high 10-year CVD risk were Malay ethnicity (Odds Ratio(OR) 0.76, 95%CI 0.63-0.92, p = 0.004), unmarried status (OR 1.55, 95%CI 1.22-1.97, p<0.001) and physically inactive (OR 0.72, 95%CI 0.55-0.95, p = 0.020). There is a need for future study to evaluate preventive strategies to improve the health of older people in order to promote healthy ageing.
    MeSH terms: Aged; Aged, 80 and over; Cardiovascular Diseases/epidemiology*; Cardiovascular Diseases/physiopathology*; Female; Humans; Hypertension/epidemiology; Malaysia/epidemiology; Male; Middle Aged; Morbidity; Surveys and Questionnaires; Risk Factors; Sex Factors; Body Mass Index; Prevalence; Logistic Models; Odds Ratio; Sedentary Lifestyle
  2. Navien TN, Thevendran R, Hamdani HY, Tang TH, Citartan M
    Biochimie, 2020 Oct 18;180:54-67.
    PMID: 33086095 DOI: 10.1016/j.biochi.2020.10.005
    Aptamers are single-stranded DNA or RNA oligonucleotides generated by SELEX that exhibit binding affinity and specificity against a wide variety of target molecules. Compared to RNA aptamers, DNA aptamers are much more stable and therefore are widely adopted in a number of applications especially in diagnostics. The tediousness and rigor associated with certain steps of the SELEX intensify the efforts to adopt in silico molecular docking approaches together with in vitro SELEX procedures in developing DNA aptamers. Inspired by these endeavors, we carry out an overview of the in silico molecular docking approaches in DNA aptamer generation, by detailing the stepwise procedures as well as shedding some light on the various softwares used. The in silico maturation strategy and the limitations of the in silico approaches are also underscored.
    MeSH terms: Computer Simulation; DNA; DNA, Single-Stranded; Family Characteristics; RNA; Software; SELEX Aptamer Technique; Aptamers, Nucleotide; Molecular Docking Simulation
  3. Basripuzi NH, Sharma RSK, Norhadila Z, Shahar ZS, Nor-Dilaila MS, Mustapha MS, et al.
    Vet Parasitol, 2020 Oct 15;287:109274.
    PMID: 33086185 DOI: 10.1016/j.vetpar.2020.109274
    This study was conducted to investigate potential interactions between the abomasal blood feeder Haemonchus contortus and the intestinal mucosal browser Trichostrongylus colubriformis among deliberately infected Boer goats. Faecal and blood samples were collected twice a week for eight weeks from 25 parasite-naïve goats. Correlation analysis and multiple linear regression models were conducted to explore the association between phenotypic variables and variables taken at necropsy. Positive associations were identified between total FEC and log T. colubriformis number (r = 0.62, p 
  4. Giribabu N, Karim K, Kilari EK, Nelli SR, Salleh N
    Inflammopharmacology, 2020 Dec;28(6):1599-1622.
    PMID: 32588370 DOI: 10.1007/s10787-020-00733-3
    Centella asiatica is claimed to have a neuroprotective effect; however, its ability to protect the cerebrum against damage in diabetes has never been identified. The aims were to identify the possibility that C. asiatica ameliorates inflammation, oxidative stress, and apoptosis in the cerebrum in diabetes. C. asiatica leave aqueous extract (C. asiatica) (50, 100, and 200 mg/kg/b.w.) were given to diabetic rats for 28 days. Changes in rats' body weight, food and water intakes, and insulin and FBG levels were monitored. Following sacrificed, cerebrum was harvested and subjected for histological, biochemical, and molecular biological analyses. The results revealed treatment with C. asiatica was able to ameliorate the loss in body weight, the increase in food and water intakes, the decrease in insulin, and the increase in FBG levels in diabetic rats. Additionally, histopathological changes in the cerebrum and levels of p38, ERK, JNK, cytosolic Nrf2, Keap-1, LPO, RAGE, and AGE levels decreased; however, PI3K, AKT, IR, IRS, GLUT-1, nuclear Nrf2, Nqo-1, Ho-1, and anti-oxidative enzymes (SOD, CAT, and GPx) levels increased in diabetic rats receiving C. asiatica. Furthermore, C. asiatica treatment also caused cerebral inflammation and apoptosis to decrease as indicated by decreased inflammatory markers (cytosolic NF-κB p65, p-Ikkβ, Ikkβ, iNOS, COX-2, TNF-α, IL-6, and IL-1β), decreased pro-apoptosis markers (Casp-3, 9, and Bax), but increased anti-apoptosis marker, Bcl-2. Activity level of Na+/K+, Mg2+, and Ca2+-ATPases in the cerebrum also increased by C. asiatica treatment. Conclusions: C. asiatica treatment helps to prevent cerebral damage and maintain near normal cerebral function in diabetes.
    MeSH terms: Administration, Oral; Animals; Antioxidants/pharmacology; Blood Glucose/drug effects; Brain/drug effects*; Brain/metabolism; Diabetes Mellitus, Experimental/drug therapy; Diabetes Mellitus, Experimental/metabolism; Diabetes Mellitus, Type 2/drug therapy*; Diabetes Mellitus, Type 2/metabolism; Inflammation/drug therapy*; Inflammation/metabolism; Insulin/metabolism; Male; Plant Extracts/pharmacology; Triterpenes/pharmacology*; Water/chemistry; Biomarkers/metabolism; Apoptosis/drug effects*; Oxidative Stress/drug effects*; Plant Leaves/chemistry; Protective Agents/pharmacology; Centella/chemistry*; Rats
  5. Rasul RM, Tamilarasi Muniandy M, Zakaria Z, Shah K, Chee CF, Dabbagh A, et al.
    Carbohydr Polym, 2020 Dec 15;250:116800.
    PMID: 33049807 DOI: 10.1016/j.carbpol.2020.116800
    Chitosan, as a biodegradable and biocompatible polymer, is characterized by anti-microbial and anti-cancer properties. It lately has received a widespread interest for use as the pulmonary particulate backbone materials of drug carrier for the treatment of infectious disease and cancer. The success of chitosan as pulmonary particulate drug carrier is a critical interplay of their mucoadhesive, permeation enhancement and site/cell-specific attributes. In the case of nanocarriers, various microencapsulation and micro-nano blending systems have been devised to equip them with an appropriate aerodynamic character to enable efficient pulmonary aerosolization and inhalation. The late COVID-19 infection is met with acute respiratory distress syndrome and cancer. Chitosan and its derivatives are found useful in combating HCoV and cancer as a function of their molecular weight, substituent type and its degree of substitution. The interest in chitosan is expected to rise in the next decade from the perspectives of drug delivery in combination with its therapeutic performance.
    MeSH terms: Anti-Infective Agents/pharmacology; Anti-Infective Agents/therapeutic use; Anti-Infective Agents/chemistry*; Antineoplastic Agents/pharmacology; Antineoplastic Agents/therapeutic use; Antineoplastic Agents/chemistry*; Biocompatible Materials/chemistry; Cell Survival/drug effects; Drug Carriers/chemistry*; Humans; Lung Neoplasms/pathology; Pneumonia, Viral/drug therapy; Pneumonia, Viral/pathology; Pneumonia, Viral/virology; Coronavirus Infections/drug therapy; Coronavirus Infections/pathology; Coronavirus Infections/virology; Chitosan/analogs & derivatives*; Pandemics
  6. Gilmore B, Ndejjo R, Tchetchia A, de Claro V, Mago E, Diallo AA, et al.
    BMJ Glob Health, 2020 Oct;5(10).
    PMID: 33051285 DOI: 10.1136/bmjgh-2020-003188
    INTRODUCTION: Community engagement has been considered a fundamental component of past outbreaks, such as Ebola. However, there is concern over the lack of involvement of communities and 'bottom-up' approaches used within COVID-19 responses thus far. Identifying how community engagement approaches have been used in past epidemics may support more robust implementation within the COVID-19 response.

    METHODOLOGY: A rapid evidence review was conducted to identify how community engagement is used for infectious disease prevention and control during epidemics. Three databases were searched in addition to extensive snowballing for grey literature. Previous epidemics were limited to Ebola, Zika, SARS, Middle East respiratory syndromeand H1N1 since 2000. No restrictions were applied to study design or language.

    RESULTS: From 1112 references identified, 32 articles met our inclusion criteria, which detail 37 initiatives. Six main community engagement actors were identified: local leaders, community and faith-based organisations, community groups, health facility committees, individuals and key stakeholders. These worked on different functions: designing and planning, community entry and trust building, social and behaviour change communication, risk communication, surveillance and tracing, and logistics and administration.

    CONCLUSION: COVID-19's global presence and social transmission pathways require social and community responses. This may be particularly important to reach marginalised populations and to support equity-informed responses. Aligning previous community engagement experience with current COVID-19 community-based strategy recommendations highlights how communities can play important and active roles in prevention and control. Countries worldwide are encouraged to assess existing community engagement structures and use community engagement approaches to support contextually specific, acceptable and appropriate COVID-19 prevention and control measures.

    MeSH terms: Communicable Disease Control*; Consumer Participation*; Humans; Pneumonia, Viral/prevention & control*; Coronavirus Infections/prevention & control*; Pandemics/prevention & control*
  7. McKimm J, Ramani S, Kusurkar RA, Fornari A, Nadarajah VD, Thampy H, et al.
    Perspect Med Educ, 2020 12;9(6):385-390.
    PMID: 33051804 DOI: 10.1007/s40037-020-00623-y
    BACKGROUND: Conversations about educational challenges and potential solutions among a globally and culturally diverse group of health professions' educators can facilitate identity formation, mentoring relationships and professional network building. The COVID-19 pandemic has made it even more important to co-create and disseminate knowledge, specifically regarding online and flexible learning formats.

    APPROACH: Based on the principles of social learning, we combined speed mentoring and world café formats to offer a virtual Zoom™ workshop, with large and small group discussions, to reach health professions' educators across the globe. The goal was to establish a psychologically safe space for dialogue regarding adaptation to online teaching-learning formats.

    EVALUATION: We aimed to establish psychological safety to stimulate thought-provoking discussions within the various small groups and obtain valuable contributions from participants. From these conversations, we were able to formulate 'hot tips' on how to adapt to (sometimes new) online teaching-learning formats while nurturing teacher and student wellbeing.

    REFLECTION: Through this virtual workshop we realized that despite contextual differences, many challenges are common worldwide. We experienced technological difficulties during the session, which needed rapid adaptation by the organising team. We encouraged, but did not pressure, participants to use video and audio during breakout discussions as we wanted them to feel safe and comfortable. The large audience size and different time zones were challenging; therefore, leadership had to be resilient and focussed. Although this virtual format was triggered by the pandemic, the format can be continued in the future to discuss other relevant global education topics.

    MeSH terms: Adaptation, Psychological; Communication; Congresses as Topic; Education, Professional/methods*; Health Occupations/education*; Humans; Learning; Teaching; Education, Distance/methods*; Mentoring
  8. Loktionov VM
    Zootaxa, 2020 May 05;4768(4):zootaxa.4768.4.5.
    PMID: 33055638 DOI: 10.11646/zootaxa.4768.4.5
    The genus Telostholus Haupt, 1929 is newly reported from Malaysia, with four species that are newly described and illustrated: T. bifasciatus Loktionov, sp. nov. (Pahang), T. clypeatus Loktionov, sp. nov. (Pahang), T. malayensis Loktionov, sp. nov. (Pahang), and T. tomentosus Loktionov, sp. nov. (Pahang). A key to the Malaysian species is given.
    MeSH terms: Animals; Malaysia; Wasps*
  9. Lan T, Yao Z, Zheng G, Wongprom P, Li S
    Zootaxa, 2020 May 14;4778(2):zootaxa.4778.2.4.
    PMID: 33055822 DOI: 10.11646/zootaxa.4778.2.4
    The genus Savarna Huber, 2005 comprises only five species, from southern Thailand, Peninsular Malaysia and Sumatra. In this study, five new species are described from Thailand: Savarna bannang sp. nov. (Yala), S. chiangmai sp. nov. (Chiangmai), S. huahin sp. nov. (Prachuap Kiri Khan), S. satun sp. nov. (Satun), S. thungsong sp. nov. (Nakhon Srithammarat). All new species are described from males and females. The distribution of S. chiangmai sp. nov. represent the northernmost record of the genus.
    MeSH terms: Animals; Female; Male; Spiders*; Thailand
  10. Volynkin AV, Singh N, ČernÝ K, Kirti JS, Datta HS
    Zootaxa, 2020 May 26;4780(3):zootaxa.4780.3.2.
    PMID: 33056511 DOI: 10.11646/zootaxa.4780.3.2
    The Miltochrista obliquilinea (Swinhoe, 1901) species-group is revised. Four new species are described: M. konta Volynkin, Černý N. Singh, sp. n. (Thailand, Laos and Vietnam), M. adelfika Volynkin, N. Singh, Černý, Kirti Datta, sp. n. (India, Myanmar, China, Thailand, Laos and Vietnam), M. stenovalva Volynkin, N. Singh, Černý, Kirti Datta, sp. n. (India and Thailand) and M. lavides Volynkin, Černý N. Singh, sp. n. (Malaysia, Thailand, Myanmar, Laos and Vietnam). The lectotype for Lyclene obliquilinea Swinhoe, 1901 is designated. Adults, male and female genitalia are illustrated.
    MeSH terms: Animals; Female; Lepidoptera*; Male
  11. Mahmood S, Kiong KC, Tham CS, Chien TC, Hilles AR, Venugopal JR
    AAPS PharmSciTech, 2020 Oct 14;21(7):285.
    PMID: 33057878 DOI: 10.1208/s12249-020-01810-0
    Currently, pharmaceutical research is directed wide range for developing new drugs for oral administration to target disease. Acyclovir formulation is having common issues of short half-life and poor permeability, causing messy treatment which results in patient incompliance. The present study formulates a lipid polymeric hybrid nanoparticles for antiviral acyclovir (ACV) agent with Phospholipon® 90G (lecithin), chitosan, and polyethylene glycol (PEG) to improve controlled release of the drugs. The study focused on the encapsulation of the ACV in lipid polymeric particle and their sustained delivery. The formulation developed for the self-assembly of chitosan and lecithin to form a shell encapsulating acyclovir, followed by PEGylation. Optimisation was performed via Box-Behnken Design (BBD), forming nanoparticles with size of 187.7 ± 3.75 nm, 83.81 ± 1.93% drug-entrapped efficiency (EE), and + 37.7 ± 1.16 mV zeta potential. Scanning electron microscopy and transmission electron microscopy images displayed spherical nanoparticles formation. Encapsulation of ACV and complexity with other physical parameters are confirmed through analysis using Fourier transform infrared spectroscopy, differential scanning calorimetry, and X-ray diffraction. Nanoparticle produced was capable of achieving 24-h sustained release in vitro on gastric and intestinal environments. Ex vivo study proved the improvement of acyclovir's apparent permeability from 2 × 10-6 to 6.46 × 10-6 cm s-1. Acyclovir new formulation was achieved to be stable up to 60 days for controlled release of the drugs. Graphical abstract.
    MeSH terms: Acyclovir/administration & dosage*; Acyclovir/pharmacokinetics; Animals; Antiviral Agents/administration & dosage*; Antiviral Agents/pharmacokinetics; Delayed-Action Preparations; Drug Compounding; Drug Stability; Intestinal Absorption; Lipids/chemistry; Particle Size; Polyethylene Glycols; Rabbits; Chitosan; Nanoparticles; Lecithins
  12. Concessao P, Bairy LK, Raghavendra AP
    Vet World, 2020 Aug;13(8):1555-1566.
    PMID: 33061227 DOI: 10.14202/vetworld.2020.1555-1566
    Background and Aim: Intoxication of arsenic in rats is known to result in neurological effects as well as liver and kidney dysfunction. Mucuna pruriens has been identified for its medicinal properties. The aim of the study was to investigate the protective effect of aqueous seed extract of M. pruriens on sodium arsenite-induced memory impairment, liver, and kidney functions in rats.

    Materials and Methods: The experiment was divided into short-term treatment (45 days) and long-term treatment (90 days), with each group divided into nine sub-groups consisting of six animals each. Sub-groups 1 and 2 served as normal, and N-acetylcysteine (NAC) controls, respectively. Sub-groups 3-9 received sodium arsenite in drinking water (50 mg/L). In addition, sub-group 4 received NAC (210 mg/kg b.wt) orally once daily, sub-groups 5-7 received aqueous seed extract of M. pruriens (350 mg/kg b.wt, 530 mg/kg b.wt, and 700 mg/kg b.wt) orally once daily and sub-groups 8 and 9 received a combination of NAC and aqueous seed extract of M. pruriens (350 mg/kg b.wt and 530 mg/kg b.wt) orally once daily. Following the treatment, the blood was drawn retro-orbitally to assess the liver (serum alanine transaminase [ALT], serum aspartate transaminase, and serum alkaline phosphatase) and kidney (serum urea and serum creatinine) functions. Learning and memory were assessed by passive avoidance test. Animals were sacrificed by an overdose of ketamine, and their Nissl stained hippocampal sections were analyzed for alterations in neural cell numbers in CA1 and CA3 regions.

    Results: In the short-term treatment, groups administered with M. pruriens 530 mg/kg b.wt alone and combination of NAC + M. pruriens 350 mg/kg b.wt exhibited a significant improvement in memory retention, less severe neurodegeneration, and decrease in serum ALT levels. In long-term treatment, groups administered with M. pruriens 700 mg/kg b.wt alone and combination of NAC+M. pruriens 350 mg/kg b.wt, respectively, showed better memory retention, decreased neural deficits, and reduced levels of kidney and liver enzymes.

    Conclusion: The seed extract of M. pruriens showed significant enhancement in memory and learning. The number of surviving neurons in the CA1 and CA3 regions also increased on treatment with M. pruriens. Serum ALT, serum urea, and serum creatinine levels showed significant improvement on long-term treatment with M. pruriens.

    MeSH terms: Acetylcysteine; Alanine Transaminase; Alkaline Phosphatase; Animals; Arsenic; Aspartate Aminotransferases; Creatinine; Ketamine; Learning; Liver; Memory; Sodium Compounds; Arsenites; Mucuna; Rats; Drinking Water
  13. Chang YJ, Pong LY, Hassan SS, Choo WS
    Access Microbiol, 2020;2(1):acmi000073.
    PMID: 33062932 DOI: 10.1099/acmi.0.000073
    This study investigated the antiviral activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against dengue virus type 2 (DENV-2). The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by sub-fractionation and Amberlite XAD16N column chromatography to obtain betacyanin fractions. The half maximum cytotoxicity concentration for betacyanin fractions from red pitahaya and red spinach on Vero cells were 4.346 and 2.287 mg ml-1, respectively. The half-maximal inhibitory concentration (IC50) of betacyanin fraction from red pitahaya was 125.8 μg ml-1 with selectivity index (SI) of 5.8. For betacyanin fraction from red spinach, the IC50 value was 14.62 µg ml-1 with SI of 28.51. Using the maximum non-toxic betacyanin concentration, direct virucidal effect against DENV-2 was obtained from betacyanin fraction from red pitahaya (IC50 of 126.70 μg ml-1; 95.0 % virus inhibition) and red spinach (IC50 value of 106.80 μg ml-1; 65.9 % of virus inhibition). Betacyanin fractions from red pitahaya and red spinach inhibited DENV-2 in vitro.
  14. Sii CKS, Lee JA, Nah SA
    Pediatr Surg Int, 2020 Dec;36(12):1407-1411.
    PMID: 33068142 DOI: 10.1007/s00383-020-04760-7
    PURPOSE: The COVID-19 pandemic has placed extraordinary demands on healthcare services worldwide. Some have reported increased viral transmission to healthcare workers during aerosol-generating procedures such as intubation. We report our experience with universal preoperative and preprocedural screening for COVID-19 in children requiring general anaesthesia with low risk of having the infection.

    METHODS: This was a data review involving children aged 

    MeSH terms: Adolescent; Anesthesia, General*; Child; Child, Preschool; Cross-Sectional Studies; Female; Humans; Infant; Infant, Newborn; Male; Pneumonia, Viral/diagnosis*; Preoperative Care/methods*; Prospective Studies; Databases, Factual; Coronavirus Infections/diagnosis*; Clinical Laboratory Techniques/methods*; Pandemics
  15. Bande F, Arshad SS, Bejo MH, Omar AR, Moeini H, Khadkodaei S, et al.
    Microb Pathog, 2020 Dec;149:104560.
    PMID: 33068733 DOI: 10.1016/j.micpath.2020.104560
    Infectious Bronchitis (IB) is an economically important avian disease that considerably threatens the global poultry industry. This is partly, as a result of its negative consequences on egg production, weight gain as well as mortality rate.The disease is caused by a constantly evolving avian infectious bronchitis virus whose isolates are classified into several serotypes and genotypes that demonstrate little or no cross protection. In order to curb the menace of the disease therefore, broad based vaccines are urgently needed. The aim of this study was to develop a recombinant DNA vaccine candidate for improved protection of avian infectious bronchitis in poultry. Using bioinformatics and molecular cloning procedures, sets of monovalent and bivalent DNA vaccine constructs were developed based on the S1 glycoprotein from classical and variants IBV strains namely, M41 and CR88 respectively. The candidate vaccine was then encapsulated with a chitosan and saponin formulated nanoparticle for enhanced immunogenicity and protective capacity. RT-PCR assay and IFAT were used to confirm the transcriptional and translational expression of the encoded proteins respectively, while ELISA and Flow-cytometry were used to evaluate the immunogenicity of the candidate vaccine following immunization of various SPF chicken groups (A-F). Furthermore, histopathological changes and virus shedding were determined by quantitative realtime PCR assay and lesion scoring procedure respectively following challenge of various subgroups with respective wild-type IBV viruses. Results obtained from this study showed that, groups vaccinated with a bivalent DNA vaccine construct (pBudCR88-S1/M41-S1) had a significant increase in anti-IBV antibodies, CD3+ and CD8+ T-cells responses as compared to non-vaccinated groups. Likewise, the bivalent vaccine candidate significantly decreased the oropharyngeal and cloacal virus shedding (p < 0.05) compared to non-vaccinated control. Chickens immunized with the bivalent vaccine also exhibited milder clinical signs as well as low tracheal and kidney lesion scores following virus challenge when compared to control groups. Collectively, the present study demonstrated that bivalent DNA vaccine co-expressing dual S1 glycoprotein induced strong immune responses capable of protecting chickens against infection with both M41 and CR88 IBV strains. Moreso, it was evident that encapsulation of the vaccine with chitosan-saponin nanoparticle further enhanced immune responses and abrogates the need for multiple booster administration of vaccine. Therefore, the bivalent DNA vaccine could serve as efficient and effective alternative strategy for the control of IB in poultry.
    MeSH terms: Animals; Antibodies, Viral/immunology; Infectious bronchitis virus/immunology*; Bronchitis/immunology; Bronchitis/prevention & control; Bronchitis/veterinary; Chickens; Immunity, Cellular; Immunization, Secondary/veterinary; Poultry Diseases/immunology*; Poultry Diseases/prevention & control; Saponins/immunology*; Saponins/chemistry; Vaccination/veterinary; Viral Vaccines/genetics; Viral Vaccines/immunology*; Viral Vaccines/chemistry; Coronavirus Infections/immunology; Coronavirus Infections/prevention & control; Coronavirus Infections/veterinary*; CD8-Positive T-Lymphocytes/immunology; Vaccines, DNA/genetics; Vaccines, DNA/immunology*; Vaccines, DNA/chemistry; Chitosan/immunology*; Chitosan/chemistry; Nanoparticles/chemistry; Cross Protection; Immunogenicity, Vaccine
  16. Morrow CJ, Kreizinger Z, Achari RR, Bekő K, Yvon C, Gyuranecz M
    Vet Microbiol, 2020 Nov;250:108840.
    PMID: 33068825 DOI: 10.1016/j.vetmic.2020.108840
    Mycoplasma synoviae (n = 26) and M. gallisepticum (n = 11) isolates were gained from 164 clinical samples collected from China, India, Indonesia, Malaysia, Philippines, Republic of Korea and Thailand. Most isolates were from commercial chicken production systems. A method of filtering (0.45 μm) samples immediately after collection was convenient allowing over a week for transit to the laboratory. Minimum inhibitory concentrations (MICs) were characterized by a broth microdilution method to enrofloxacin, difloxacin, oxytetracycline, chlortetracycline, doxycycline, tylosin, tilmicosin, tylvalosin, tiamulin, florfenicol, lincomycin, spectinomycin and lincomycin and spectinomycin combination (1:2). Increased MICs to various antimicrobials were seen in different isolates but appeared largely unrelated to the antimicrobial treatment histories. Overall, the results were similar to other MIC surveys around the world. Generally, low MICs to tetracyclines, tiamulin and tylvalosin were observed. Increased tilmicosin MICs were observed in both M. synoviae and M. gallisepticum isolates (≥64 μg/ml MIC90 values) and this was seen in all isolates with high tylosin MICs. Increases in lincomycin MICs were mostly associated with increases in tilmicosin MICs. The results also suggested that antimicrobial use after mycoplasma vaccination may interfere with vaccine strain persistence and efficacy (field strains were more commonly observed in flocks that had treatments after vaccination) and this area warrants more investigation. The study shows that isolation and MIC determination can be done from remote locations and suggests that this may provide information that will allow more effective use of antimicrobials or other methods of control of avian mycoplasma in chickens (e.g. live vaccines) and therefore more responsible use of antimicrobials from a one health perspective.
    MeSH terms: Animals; Anti-Bacterial Agents/pharmacology*; Asia; Chickens; Microbial Sensitivity Tests; Mycoplasma Infections/drug therapy; Mycoplasma Infections/veterinary*; Poultry Diseases/drug therapy; Poultry Diseases/microbiology*; Mycoplasma synoviae/drug effects*; Mycoplasma synoviae/pathogenicity*
  17. Ong SC, Vasan Thakumar A, Ooi GS, Shafie AA, Ahmad Hassali MA
    Int J Pharm Pract, 2020 Dec;28(6):579-590.
    PMID: 33074560 DOI: 10.1111/ijpp.12673
    OBJECTIVE: Unregistered medications which are lacking regulation in terms of safety, efficacy and quality may cause unpredictable adverse drug reactions (ADRs) in consumers. Therefore, this study aimed to assess the public knowledge, attitude and practice of consuming and purchasing unregistered health products in Malaysia.

    METHODS: A cross-sectional study involving members of the Malaysian public was carried out using the convenience sampling method. Descriptive statistics were used to summarise the socio-demographic characteristics of the respondents. Associations between knowledge items/scores and other items were assessed using Spearman's rank correlations and Cramer's V. Regression analyses were carried out to determine whether the socio-demographic characteristics of the respondents influenced knowledge and practice relating to unregistered medications.

    KEY FINDINGS: A total of 649 respondents completed the questionnaire with the majority being female (66.1%), unmarried (66.5%), Malay (52.5%) and possessing a bachelor's degree (53.5%). The knowledge of the public surveyed regarding unregistered (unlicensed) medications was lacking, especially in being able to identify a registered health product in Malaysia and formally complaining if necessary. The respondents agreed that currently, there are insufficient laws and educational programmes to tackle the issue. The respondents exhibited good practice habits by purchasing their medications from healthcare professionals. Mean knowledge score was positively correlated to practice scores at rs  = 0.423 (P-value 

    MeSH terms: Adolescent; Adult; Age Factors; Commerce/legislation & jurisprudence*; Cross-Sectional Studies; Drug and Narcotic Control; Educational Status; Female; Humans; Income; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Middle Aged; Surveys and Questionnaires; Street Drugs/adverse effects*; Street Drugs/legislation & jurisprudence; Young Adult; Drug-Related Side Effects and Adverse Reactions/epidemiology*
  18. Mousa A, Al-Taiar A, Anstey NM, Badaut C, Barber BE, Bassat Q, et al.
    PLoS Med, 2020 Oct;17(10):e1003359.
    PMID: 33075101 DOI: 10.1371/journal.pmed.1003359
    BACKGROUND: Delay in receiving treatment for uncomplicated malaria (UM) is often reported to increase the risk of developing severe malaria (SM), but access to treatment remains low in most high-burden areas. Understanding the contribution of treatment delay on progression to severe disease is critical to determine how quickly patients need to receive treatment and to quantify the impact of widely implemented treatment interventions, such as 'test-and-treat' policies administered by community health workers (CHWs). We conducted a pooled individual-participant meta-analysis to estimate the association between treatment delay and presenting with SM.

    METHODS AND FINDINGS: A search using Ovid MEDLINE and Embase was initially conducted to identify studies on severe Plasmodium falciparum malaria that included information on treatment delay, such as fever duration (inception to 22nd September 2017). Studies identified included 5 case-control and 8 other observational clinical studies of SM and UM cases. Risk of bias was assessed using the Newcastle-Ottawa scale, and all studies were ranked as 'Good', scoring ≥7/10. Individual-patient data (IPD) were pooled from 13 studies of 3,989 (94.1% aged <15 years) SM patients and 5,780 (79.6% aged <15 years) UM cases in Benin, Malaysia, Mozambique, Tanzania, The Gambia, Uganda, Yemen, and Zambia. Definitions of SM were standardised across studies to compare treatment delay in patients with UM and different SM phenotypes using age-adjusted mixed-effects regression. The odds of any SM phenotype were significantly higher in children with longer delays between initial symptoms and arrival at the health facility (odds ratio [OR] = 1.33, 95% CI: 1.07-1.64 for a delay of >24 hours versus ≤24 hours; p = 0.009). Reported illness duration was a strong predictor of presenting with severe malarial anaemia (SMA) in children, with an OR of 2.79 (95% CI:1.92-4.06; p < 0.001) for a delay of 2-3 days and 5.46 (95% CI: 3.49-8.53; p < 0.001) for a delay of >7 days, compared with receiving treatment within 24 hours from symptom onset. We estimate that 42.8% of childhood SMA cases and 48.5% of adult SMA cases in the study areas would have been averted if all individuals were able to access treatment within the first day of symptom onset, if the association is fully causal. In studies specifically recording onset of nonsevere symptoms, long treatment delay was moderately associated with other SM phenotypes (OR [95% CI] >3 to ≤4 days versus ≤24 hours: cerebral malaria [CM] = 2.42 [1.24-4.72], p = 0.01; respiratory distress syndrome [RDS] = 4.09 [1.70-9.82], p = 0.002). In addition to unmeasured confounding, which is commonly present in observational studies, a key limitation is that many severe cases and deaths occur outside healthcare facilities in endemic countries, where the effect of delayed or no treatment is difficult to quantify.

    CONCLUSIONS: Our results quantify the relationship between rapid access to treatment and reduced risk of severe disease, which was particularly strong for SMA. There was some evidence to suggest that progression to other severe phenotypes may also be prevented by prompt treatment, though the association was not as strong, which may be explained by potential selection bias, sample size issues, or a difference in underlying pathology. These findings may help assess the impact of interventions that improve access to treatment.

    MeSH terms: Antimalarials/therapeutic use; Benin/epidemiology; Community Health Workers; Gambia/epidemiology; Humans; Malaria/drug therapy; Malaria/epidemiology; Malaysia/epidemiology; Mozambique/epidemiology; Plasmodium falciparum/pathogenicity; Tanzania/epidemiology; Uganda/epidemiology; Yemen/epidemiology; Zambia/epidemiology; Malaria, Falciparum/drug therapy*; Malaria, Falciparum/epidemiology*; Disease Progression; Time-to-Treatment/economics
  19. Ng CFS, Seposo XT, Moi ML, Tajudin MABA, Madaniyazi L, Sahani M
    Int J Infect Dis, 2020 Dec;101:409-411.
    PMID: 33075527 DOI: 10.1016/j.ijid.2020.10.027
    The first wave of COVID-19 epidemic began in late January in Malaysia and ended with a very small final size. The second wave of infections broke out in late February and grew rapidly in the first 3 weeks. Authorities in the country responded quickly with a series of control strategies collectively known as the Movement Control Order (MCO) with different levels of intensity matching the progression of the epidemic. We examined the characteristics of the second wave and discussed the key control strategies implemented in the country. In the second wave, the epidemic doubled in size every 3.8 days (95% confidence interval [CI]: 3.3, 4.5) in the first month and decayed slowly after that with a halving time of approximately 3 weeks. The time-varying reproduction number Rt peaked at 3.1 (95% credible interval: 2.7, 3.5) in the 3rd week, declined sharply thereafter and stayed below 1 in the last 3 weeks of April, indicating low transmissibility approximately 3 weeks after the MCO. Experience of the country suggests that adaptive triggering of distancing policies combined with a population-wide movement control measure can be effective in suppressing transmission and preventing a rebound.
    MeSH terms: Humans; Malaysia/epidemiology; Epidemics/prevention & control; Epidemics/statistics & numerical data; Pandemics/prevention & control; Pandemics/statistics & numerical data
  20. Pati S, Chatterji A, Dash BP, Raveen Nelson B, Sarkar T, Shahimi S, et al.
    Polymers (Basel), 2020 Oct 15;12(10).
    PMID: 33076234 DOI: 10.3390/polym12102361
    Natural product extraction is ingenuity that permits the mass manufacturing of specific products in a cost-effective manner. With the aim of obtaining an alternative chitosan supply, the carapace of dead horseshoe crabs seemed feasible. This sparked an investigation of the structural changes and antioxidant capacity of horseshoe crab chitosan (HCH) by γ-irradiation using 60Co source. Chitosan was extracted from the horseshoe crab (Tachypleus gigas; Müller) carapace using heterogeneous chemical N-deacetylation of chitin, followed by the irradiation of HCH using 60Co at a dose-dependent rate of 10 kGy/hour. The average molecular weight was determined by the viscosimetric method. Regarding the chemical properties, the crystal-like structures obtained from γ-irradiated chitosan powders were determined using Fourier transfer infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analyses. The change in chitosan structure was evident with dose-dependent rates between 10 and 20 kGy/hour. The antioxidant properties of horseshoe crab-derived chitosan were evaluated in vitro. The 20 kGy γ-irradiation applied to chitosan changed the structure and reduced the molecular weight, providing sufficient degradation for an increase in antioxidant activity. Our findings indicate that horseshoe crab chitosan can be employed for both scald-wound healing and long-term food preservation due to its buffer-like and radical ion scavenging ability.
    MeSH terms: Animals; Antioxidants; Biological Products; Chitin; Cobalt Radioisotopes; Cost-Benefit Analysis; Food Preservation; Gamma Rays; Horseshoe Crabs; Molecular Weight; Powders; X-Ray Diffraction; Spectroscopy, Fourier Transform Infrared; Chitosan; Animal Shells
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