Browse publications by year: 2021

  1. Vaithanomsat P, Kongsin K, Trakunjae C, Boonyarit J, Jarerat A, Sudesh K, et al.
    Polymers (Basel), 2021 May 26;13(11).
    PMID: 34073164 DOI: 10.3390/polym13111733
    This paper is aimed at investigating the usage of biosynthesized poly(3-hydroxybutyrate) (P(3-HB)) for a coating on pineapple leaf fiber paper (PLFP). For this purpose, (P(3-HB)) was produced by Rhodococcus pyridinivorans BSRT1-1, a highly potential P(3-HB) producing bacterium, with a weight-average molecular weight (Mw) of 6.07 × 10 -5 g/mol. This biosynthesized P(3-HB) at 7.5% (w/v) was then coated on PLFP through the dip-coating technique with chloroform used as a solvent. The respective coated PLFP showed that P(3-HB) could be well coated all over on the PLFP surface as confirmed by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The brightness and mechanical properties of PLFP could be improved by coating with biosynthesized P(3-HB) in comparison to commercially available P(3-HB) and non-coated PLFP. Furthermore, coating of P(3-HB) significantly increased the water drop penetration time on the surface of PLFP and was similar to that of the commercial P(3-HB) with the same content. The results showed that all the coated PLPF samples can be degraded under the soil burial test conditions. We have demonstrated that the P(3-HB) coated PLFP paper has the ability to prevent water drop penetration and could undergo biodegradation. Taken together, the P(3-HB) coated PLFP can be applied as a promising biodegradable paper packaging.
  2. Abdila SR, Abdullah MMAB, Ahmad R, Rahim SZA, Rychta M, Wnuk I, et al.
    Materials (Basel), 2021 May 26;14(11).
    PMID: 34073169 DOI: 10.3390/ma14112833
    This study intended to address the problem of damaged (collapsed, cracked and decreased soil strength) road pavement structure built on clay soil due to clay soil properties such as low shear strength, high soil compressibility, low soil permeability, low soil strength, and high soil plasticity. Previous research reported that ground granulated blast slag (GGBS) and fly ash can be used for clay soil stabilizations, but the results of past research indicate that the road pavement construction standards remained unfulfilled, especially in terms of clay's subgrade soil. Due to this reason, this study is carried out to further investigate soil stabilization using GGBS and fly ash-based geopolymer processes. This study investigates the effects of GGBS and ratios of fly ash (solid) to alkaline activator (liquid) of 1:1, 1.5:1, 2:1, 2.5:1, and 3:1, cured for 1 and 7 days. The molarity of sodium hydroxide (NaOH) and the ratio of sodium silicate (Na2SiO3) to sodium hydroxide (NaOH) was fixed at 10 molar and 2.0 weight ratio. The mechanical properties of the soil stabilization based geopolymer process were tested using an unconfined compression test, while the characterization of soil stabilization was investigated using the plastic limit test, liquid limit test, scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results showed that the highest strength obtained was 3.15 MPA with a GGBS to alkaline activator ratio of 1.5 and Na2SiO3 to NaOH ratio of 2.0 at 7 days curing time. These findings are useful in enhancing knowledge in the field of soil stabilization-based geopolymer, especially for applications in pavement construction. In addition, it can be used as a reference for academicians, civil engineers, and geotechnical engineers.
  3. Hatmal MM, Al-Hatamleh MAI, Olaimat AN, Hatmal M, Alhaj-Qasem DM, Olaimat TM, et al.
    Vaccines (Basel), 2021 May 26;9(6).
    PMID: 34073382 DOI: 10.3390/vaccines9060556
    BACKGROUND: Since the coronavirus disease 2019 (COVID-19) was declared a pandemic, there was no doubt that vaccination is the ideal protocol to tackle it. Within a year, a few COVID-19 vaccines have been developed and authorized. This unparalleled initiative in developing vaccines created many uncertainties looming around the efficacy and safety of these vaccines. This study aimed to assess the side effects and perceptions following COVID-19 vaccination in Jordan.

    METHODS: A cross-sectional study was conducted by distributing an online survey targeted toward Jordan inhabitants who received any COVID-19 vaccines. Data were statistically analyzed and certain machine learning (ML) tools, including multilayer perceptron (MLP), eXtreme gradient boosting (XGBoost), random forest (RF), and K-star were used to predict the severity of side effects.

    RESULTS: A total of 2213 participants were involved in the study after receiving Sinopharm, AstraZeneca, Pfizer-BioNTech, and other vaccines (38.2%, 31%, 27.3%, and 3.5%, respectively). Generally, most of the post-vaccination side effects were common and non-life-threatening (e.g., fatigue, chills, dizziness, fever, headache, joint pain, and myalgia). Only 10% of participants suffered from severe side effects; while 39% and 21% of participants had moderate and mild side effects, respectively. Despite the substantial variations between these vaccines in the presence and severity of side effects, the statistical analysis indicated that these vaccines might provide the same protection against COVID-19 infection. Finally, around 52.9% of participants suffered before vaccination from vaccine hesitancy and anxiety; while after vaccination, 95.5% of participants have advised others to get vaccinated, 80% felt more reassured, and 67% believed that COVID-19 vaccines are safe in the long term. Furthermore, based on the type of vaccine, demographic data, and side effects, the RF, XGBoost, and MLP gave both high accuracies (0.80, 0.79, and 0.70, respectively) and Cohen's kappa values (0.71, 0.70, and 0.56, respectively).

    CONCLUSIONS: The present study confirmed that the authorized COVID-19 vaccines are safe and getting vaccinated makes people more reassured. Most of the post-vaccination side effects are mild to moderate, which are signs that body's immune system is building protection. ML can also be used to predict the severity of side effects based on the input data; predicted severe cases may require more medical attention or even hospitalization.

  4. Zakaria Z, Zulkafflee NS, Mohd Redzuan NA, Selamat J, Ismail MR, Praveena SM, et al.
    Plants (Basel), 2021 May 26;10(6).
    PMID: 34073642 DOI: 10.3390/plants10061070
    Rice is a worldwide staple food and heavy metal contamination is often reported in rice production. Heavy metal can originate from natural sources or be present through anthropogenic contamination. Therefore, this review summarizes the current status of heavy metal contamination in paddy soil and plants, highlighting the mechanism of uptake, bioaccumulation, and health risk assessment. A scoping search employing Google Scholar, Science Direct, Research Gate, Scopus, and Wiley Online was carried out to build up the review using the following keywords: heavy metals, absorption, translocation, accumulation, uptake, biotransformation, rice, and human risk with no restrictions being placed on the year of study. Cadmium (Cd), arsenic (As), and lead (Pb) have been identified as the most prevalent metals in rice cultivation. Mining and irrigation activities are primary sources, but chemical fertilizer and pesticide usage also contribute to heavy metal contamination of paddy soil worldwide. Further to their adverse effect on the paddy ecosystem by reducing the soil fertility and grain yield, heavy metal contamination represents a risk to human health. An in-depth discussion is further offered on health risk assessments by quantitative measurement to identify potential risk towards heavy metal exposure via rice consumption, which consisted of in vitro digestion models through a vital ingestion portion of rice.
  5. Salleh RM, Kuan G, Aziz MNA, Rahim MRA, Rahayu T, Sulaiman S, et al.
    Nutrients, 2021 May 24;13(6).
    PMID: 34073684 DOI: 10.3390/nu13061783
    BACKGROUND: Reports of probiotic consumption on athletes' performance are debatable due to their equivocal results. There is a need for more evidence on the effects of probiotic intake on psychological state and fitness level. Thus, this study determined the effects of daily probiotic consumption on competitive anxiety, perceived stress and mood among university badminton players, besides their fitness like aerobic capacity, strength, speed, leg power and agility.

    METHODS: Thirty university badminton players aged from 19 to 22 years old were randomly divided equally into two groups, where the probiotic group (PG; n = 15) received a drink that contained Lactobacillus casei Shirota (3 × 1010 CFU) and placebo group (CG; n = 15) a placebo drink for six weeks. Anxiety, stress and mood levels were determined using the CSAI-2R, PSS and BRUMS questionnaires, respectively. Fitness levels were measured using by subjecting the players to 20-m shuttle runs (aerobic capacity), handgrips (muscular strength), vertical jumps (leg power), 40-m dash (speed) and T-test (agility). The Student's t-test (p < 0.05) was used to determine the differences between PG and CG players.

    RESULTS: After six weeks, the anxiety and stress levels of PG players significantly decreased by 16% (p < 0.001) and 20% (p < 0.001), respectively, but there were no significant changes detected in CG players. Supplementation of probiotics also improved aerobic capacity in PG players by 5.9% (p < 0.001) but did not influence the speed, strength, leg power and agility.

    CONCLUSIONS: Probiotics supplementation showed improved aerobic capacity and relieve anxiety and stress. However, further studies need to be carried out to determine the mechanisms through which probiotic intake produces these effects.

    MeSH terms: Adolescent; Affect; Anxiety/drug therapy*; Humans; Lactobacillus casei; Physical Fitness*; Surveys and Questionnaires; Stress, Psychological/drug therapy*; Universities; Exercise; Racquet Sports*; Treatment Outcome; Dietary Supplements; Probiotics/therapeutic use*; Muscle Strength; Athletic Performance; Young Adult
  6. Harussani MM, Sapuan SM, Rashid U, Khalina A
    Polymers (Basel), 2021 May 24;13(11).
    PMID: 34073691 DOI: 10.3390/polym13111707
    Slow pyrolysis using a batch reactor at 450 °C was applied to the polypropylene (PP) powder derived from Coronavirus Disease 2019 (COVID-19) isolation gown waste to yield char briquettes, using sugar palm starch (SPS) and a manual hydraulic press. These studies are significant because of reductions in plastic waste from the preparation of barbecue coal due to environmental sustainability. The results presented here include the physical, morphological, thermal, combustion, and mechanical properties of char when reinforced with various percentages of SPS loadings (0, 10, 20, 30, and 40%), which act as a matrix/binder to produce char/sugar palm starch (C/SPS) composites. The physical and morphological characteristics of C/SPS composites were determined using Fourier transform infrared (FTIR) and field emission scanning electron microscopy (FESEM). On the other hand, the thermal and combustion properties of the C/SPS briquettes were studied via thermogravimetric and bomb calorimeter analysis. The results show that the compressive strength of the briquettes increased as the SPS loading increased, whereas the higher heating values (HHV) reduced. The findings indicate that C-80/SPS-20 briquettes presented excellent combustion characteristics (1,761,430 J/g) with satisfactory mechanical strength (1.463 MPa) in the compression test. Thus, C-80/SPS-20 briquettes are the most suitable composites for domestic and commercial uses.
  7. Bilema M, Aman MY, Hassan NA, Memon ZA, Omar HA, Yusoff NIM, et al.
    Materials (Basel), 2021 May 24;14(11).
    PMID: 34073812 DOI: 10.3390/ma14112781
    Researchers are exploring the utilisation of reclaimed asphalt pavement (RAP) as a recycled material to determine the performance of non-renewable natural aggregates and other road products such as asphalt binder, in the construction and rehabilitation stage of asphalt pavements. The addition of RAP in asphalt mixtures is a complex process and there is a need to understand the design of the asphalt mixture. Some of the problems associated with adding RAP to asphalt mixtures are moisture damage and cracking damage caused by poor adhesion between the aggregates and asphalt binder. There is a need to add rejuvenators to the recycled mixture containing RAP to enhance its performance, excepting the rutting resistance. This study sought to improve asphalt mixture performance and mechanism by adding waste frying oil (WFO) and crumb rubber (CR) to 25 and 40% of the RAP content. Moreover, the utilisation of CR and WFO improved pavement sustainability and rutting performance. In addition, this study prepared five asphalt mixture samples and compared their stiffness, moisture damage and rutting resistance with the virgin asphalt. The results showed enhanced stiffness and rutting resistance of the RAP but lower moisture resistance. The addition of WFO and CR restored the RAP properties and produced rutting resistance, moisture damage and stiffness, which were comparable to the virgin asphalt mixture. All waste and virgin materials produce homogeneous asphalt mixtures, which influence the asphalt mixture performance. The addition of a high amount of WFO and a small amount of CR enhanced pavement sustainability and rutting performance.
    MeSH terms: Tissue Adhesions; Hydrocarbons; Rubber; Recycling
  8. Herdiana Y, Wathoni N, Shamsuddin S, Joni IM, Muchtaridi M
    Polymers (Basel), 2021 May 24;13(11).
    PMID: 34074020 DOI: 10.3390/polym13111717
    Breast cancer remains one of the world's most dangerous diseases because of the difficulty of finding cost-effective and specific targets for effective and efficient treatment methods. The biodegradability and biocompatibility properties of chitosan-based nanoparticles (ChNPs) have good prospects for targeted drug delivery systems. ChNPs can transfer various antitumor drugs to targeted sites via passive and active targeting pathways. The modification of ChNPs has attracted the researcher to the loading of drugs to targeted cancer cells. The objective of our review was to summarize and discuss the modification in ChNPs in delivering anticancer drugs against breast cancer cells from published papers recorded in Scopus, PubMed, and Google Scholar. In order to improve cellular uptake, drug accumulation, cytotoxicity, and selectivity, we examined different kinds of modification of ChNPs. Notably, these forms of ChNPs use the characteristics of the enhanced permeability and retention (EPR) effect as a proper parameter and different biological ligands, such as proteins, peptides, monoclonal antibodies, and small particles. In addition, as a targeted delivery system, ChNPs provided and significantly improved the delivery of drugs into specific breast cancer cells (MDA-MB-231, 4T1 cells, SK-BR-3, MCF-7, T47D). In conclusion, a promising technique is presented for increasing the efficacy, selectivity, and effectiveness of candidate drug carriers in the treatment of breast cancer.
  9. Hamid NJA, Kadir AA, Hashar NNH, Pietrusiewicz P, Nabiałek M, Wnuk I, et al.
    Materials (Basel), 2021 May 24;14(11).
    PMID: 34074057 DOI: 10.3390/ma14112800
    Wastewater treatment activities in the chemical industry have generated abundant gypsum waste, classified as scheduled waste (SW205) under the Environmental Quality Regulations 2005. The waste needs to be disposed into a secure landfill due to the high heavy metals content which is becoming a threat to the environment. Hence, an alternative disposal method was evaluated by recycling the waste into fired clay brick. The brick samples were incorporated with different percentages of gypsum waste (0% as control, 10, 20, 30, 40 and 50%) and were fired at 1050 °C using 1 °C per minute heating rate. Shrinkage, dry density, initial rate of suction (IRS) and compressive strength tests were conducted to determine the physical and mechanical properties of the brick, while the synthetic precipitation leaching procedure (SPLP) was performed to scrutinize the leachability of heavy metals from the crushed brick samples. The results showed that the properties would decrease through the incorporation of gypsum waste and indicated the best result at 10% of waste utilization with 47.5% of shrinkage, 1.37% of dry density, 22.87% of IRS and 28.3% of compressive strength. In addition, the leachability test highlighted that the concentrations of Fe and Al was significantly reduced up to 100% from 4884 to 3.13 ppm (Fe) and from 16,134 to 0.81 ppm (Al), respectively. The heavy metals content in the bricks were oxidized during the firing process, which signified the successful remediation of heavy metals in the samples. Based on the permissible incorporation of gypsum waste into fired clay brick, this study promised a more green disposing method for gypsum waste, and insight as a potential towards achieving a sustainable end product.
  10. Kong YL, Anis-Syakira J, Jawahir S, R'ong Tan Y, Rahman NHA, Tan EH
    BMC Public Health, 2021 Jun 01;21(1):1033.
    PMID: 34074275 DOI: 10.1186/s12889-021-11022-1
    BACKGROUND: The increase in the elderly population, chronic and degenerative diseases, as well as accidents at work and on the road in Malaysia would result in an increased demand for informal care. This paper aimed to determine the associated factors of informal caregiving and its effects on health, work and social activities of adult informal caregivers in Malaysia.

    METHODS: The data from the 2019 National Health and Morbidity Survey (NHMS), a nationwide cross-sectional survey with a two-stage stratified random sampling design, was used in this research. The study included respondents who were 18 years and older (n = 11,674). Data were obtained via face-to-face interviews using validated questionnaires. Descriptive and complex sample logistic regression analyses were employed as appropriate.

    RESULTS: 5.7% of the adult population were informal caregivers. Provision of informal care were significantly associated with the female sex (OR = 1.52, 95% CI [1.21, 1.92]), those aged 36-59 years (OR = 1.61, 95% CI [1.15, 2.25]), and those who reported illness in the past 2 weeks (OR = 1.79, 95% CI [1.38, 2.33]). The risk of having their health affected were associated with female caregivers (OR = 3.63, 95% CI [1.73, 7.61]), those who received training (OR = 2.10, 95% CI [1.10, 4.00]) and those who provided care for 2 years or more (OR = 1.91, 95% CI [1.08, 3.37]). The factors associated with the effects on work were ethnicity, received training and had no assistance to provide the care. In terms of effect on social activities, female caregivers (OR = 1.96, 95% CI [1.04, 3.69]) and caregivers who received training were more likely (OR = 2.19, 95% CI [1.22, 3.93]) to have their social activities affected.

    CONCLUSION: Our study revealed that sex, age, and self-reported illness were factors associated with being an informal caregiver in Malaysia. Informal caregivers faced effects on their health, work, and social activities which may be detrimental to their well-being. This understanding is crucial for planning support for caregivers.

    MeSH terms: Accidents; Adult; Aged; Cross-Sectional Studies; Ethnic Groups; Female; Patient Care; Health Surveys; Humans; Malaysia; Middle Aged; Morbidity; Surveys and Questionnaires; Social Behavior; Logistic Models; Caregivers; Self Report
  11. Citartan M
    Talanta, 2021 Sep 01;232:122436.
    PMID: 34074421 DOI: 10.1016/j.talanta.2021.122436
    Dot blot assays have always been associated with antibodies as the main molecular recognition element, which are widely employed in a myriad of diagnostic applications. With the rising of aptamers as the equivalent molecular recognition elements of antibodies, dot blot assays are also one of the diagnostic avenues that should be scrutinized for their amenability with aptamers as the potential surrogates of antibodies. In this review, the stepwise procedures of an aptamer-based dot blot assays are underscored before reviewing the existing aptamer-based dot blot assays developed so far. Most of the applications center on monitoring the progress of SELEX and as the validatory assays to assess the potency of aptamer candidates. For the purpose of diagnostics, the current effort is still languid and as such possible suggestions to galvanize the move to spur the aptamer-based dot blot assays to a point-of-care arena are discussed.
    MeSH terms: Antibodies; Biological Assay; Oligonucleotides; Point-of-Care Systems
  12. Mohamad-Zainal NSL, Ramli N, Zolkefli N, Mustapha NA, Hassan MA, Maeda T
    J Biosci Bioeng, 2021 Aug;132(2):174-182.
    PMID: 34074597 DOI: 10.1016/j.jbiosc.2021.04.014
    Alcaligenaceae and Chromatiaceae were previously reported as the specific pollution bioindicators in the receiving river water contaminated by palm oil mill effluent (POME) final discharge. Considering the inevitable sensitivity of bacteria under environmental stresses, it is crucial to assess the survivability of both bacteria in the fluctuated environmental factors, proving their credibility as POME pollution bioindicators in the environment. In this study, the survivability of Alcaligenaceae and Chromatiaceae from facultative pond, algae (aerobic) pond and final discharge were evaluated under varying sets of temperature (25-40°C), pH (pH 7-9) and low/high total suspended solid (TSS) contents of POME collected during low/high crop seasons of oil palm, respectively. Following treatment, the viability status and compositions of the bacterial community were assessed using flow cytometry-based assay and high-throughput Illumina MiSeq, respectively, in correlation with the changes of physicochemical properties. The changes in temperature, pH and TSS indeed changed the physicochemical properties of POME. The functionality of bacterial cells was also shifted where the viable cells and high nucleic acid contents reduced at elevated levels of temperature and pH but increased at high TSS content. Interestingly, the Alcaligenaceae and Chromatiaceae continuously detected in the samples which accounted for more than 0.5% of relative abundance, with a positive correlation with biological oxygen demand (BOD5) concentration. Therefore, either Alcaligenaceae or Chromatiaceae or both could be regarded as the reliable and specific bacterial indicators to indicate the pollution in river water due to POME final discharge despite the fluctuations in temperature, pH and TSS.
    MeSH terms: Chromatiaceae*; Hydrogen-Ion Concentration; Industrial Waste; Plant Oils; Temperature; Waste Disposal, Fluid; Alcaligenaceae*
  13. Leow YC, Roslani AC, Xavier RG, Lee FY
    Indian J Surg, 2021 May 27.
    PMID: 34075282 DOI: 10.1007/s12262-021-02945-5
    Neoadjuvant therapy is the gold standard treatment of locally advanced rectal cancer. It may induce complete sterilization of tumor cell and decreases its local recurrence rate. While 15-20% of patients were found to have pathological complete response (pCR) with combined multimodal therapy, Asian data were generally scarce. pCR rate can indicate the suitability of applying the "watch-and-wait" strategy, which advocates deferment of surgery that can alleviate surgery-associated morbidity.To determine the percentage of pCR of rectal cancer after neoadjuvant therapy. Patients diagnosed with rectal cancer underwent treatment from 2013 to 2017 were retrieved retrospectively. Demographic data, tumor localization, pre- and post-operative pathological reports, neoadjuvant therapy, and pCR status were collected from patients' records. A total of 242 out of 259 patients were treated with definitive rectal surgery. Mean age was 67.1 years old. Chinese ethnicity and male gender were predominant (n = 131, 54.1% and n = 146, 64.3% respectively). More than half (n = 124, 51.2%) had tumor located at mid or low rectum. Histologically, moderate differentiated adenocarcinoma was predominant (n = 227, 93.8%). Merely half (n = 123, 50.8%) of the patients received neoadjuvant chemoradiation therapy, but only 12 (9.8%) had a pCR. From follow-up on these 12 pCR patients, most had 2-year disease-free survival but 1 (8.3%) of the pCR had distant metastasis within 1-year post-surgery. The pathological complete response rate in our center was lower than reported. Stringent patient selection with close follow-up for patients should be carried out if the "watch-and-wait" strategy is implemented in our population.
  14. Usmani RSA, Pillai TR, Hashem IAT, Marjani M, Shaharudin R, Latif MT
    Environ Sci Pollut Res Int, 2021 Oct;28(40):56759-56771.
    PMID: 34075501 DOI: 10.1007/s11356-021-14305-7
    Air pollution has a serious and adverse effect on human health, and it has become a risk to human welfare and health throughout the globe. One of the major effects of air pollution on health is hospitalizations associated with air pollution. Recently, the estimation and prediction of air pollution-based hospitalization is carried out using artificial intelligence (AI) and machine learning (ML) techniques, i.e., deep learning and long short-term memory (LSTM). However, there is ample room for improvement in the available applied methodologies to estimate and predict air pollution-based hospital admissions. In this paper, we present the modeling and analysis of air pollution and cardiorespiratory hospitalization. This study aims to investigate the association between cardiorespiratory hospitalization and air pollution, and predict cardiorespiratory hospitalization based on air pollution using the artificial intelligence (AI) techniques. We propose the enhanced long short-term memory (ELSTM) model and provide a comparison with other AI techniques, i.e., LSTM, DL, and vector autoregressive (VAR). This study was conducted at seven study locations in Klang Valley, Malaysia. The utilized dataset contains the data from January 2006 to December 2016 for five study locations, i.e., Klang (KLN), Shah Alam (SA), Putrajaya (PUJ), Petaling Jaya (PJ), and Cheras, Kuala Lumpur (CKL). The dataset for Banting contains data from April 2010 to December 2016, and the data for Batu Muda, Kuala Lumpur, contains data from January 2009 to December 2016. The prediction results show that the ELSTM model performed significantly better than other models in all study locations, with the best RMSE scores in Klang study location (ELSTM: 0.002, LSTM: 0.013, DL: 0.006, VAR: 0.066). The results also indicated that the proposed ELSTM model was able to detect and predict the trends of monthly hospitalization significantly better than the LSTM and other models in the study. Hence, we can conclude that we can utilize AI techniques to accurately predict cardiorespiratory hospitalization based on air pollution in Klang Valley, Malaysia.
    MeSH terms: Machine Learning; Air Pollution*; Artificial Intelligence; Hospitalization; Humans; Malaysia
  15. Hoh SM, Wahab MYA, Hisham AN, Guest GD, Watters DAK
    ANZ J Surg, 2021 Jun 01.
    PMID: 34075677 DOI: 10.1111/ans.16986
    BACKGROUND: Surgical conditions form a significant proportion of the global burden of disease. Since the 2015 World Health Assembly resolution A68.15, there is recognition that the provision of essential surgical care is an integral part of universal access to health care. The Lancet Commission on Global Surgery proposed its first surgical indicator to measure a population's access to the Bellwether procedures (laparotomy, caesarean section and treatment of open fracture) within two hours. Bellwether access is a proxy for emergency and essential surgical care. This project aims to map essential surgical access to the Bellwether procedures in Malaysia.

    METHODS: The location and capability of hospitals to perform the Bellwether procedures was obtained from the Ministry of Health (MoH) and MoH hospital specific websites. The Malaysian population data were retrieved from the national department of statistics. Times for patients to travel to hospital were calculated by combining manual contouring and geospatial mapping.

    RESULTS: There were 49 Bellwether-capable MoH hospitals serving a national population of 32.5 million. Overall 94% of Malaysia's population have access to the Bellwethers within two hours. This coverage is universal in West (Peninsular) Malaysia, but there is only 73% coverage in East Malaysia, with 1.8 million residents of Sabah and Sarawak not having timely access. Malaysia's Bellwether capacity compares well with other countries in World Health Organisation's Western Pacific region.

    CONCLUSION: There is good access to essential and emergency surgical services in Malaysia. The incomplete access for 1.8 million people in East Malaysia will inform national surgical planning.

    MeSH terms: Biometry; Cesarean Section; Emergencies; Female; Fractures, Open; Health Services Accessibility; Hospitals; Humans; Laparotomy; Malaysia; Pregnancy; Global Health; World Health Organization; Global Burden of Disease
  16. Sun ZJ, Zhu W, Zhu WB, Zhao CL, Liao CL, Zou B, et al.
    Zool Res, 2021 Jul 18;42(4):412-416.
    PMID: 34075734 DOI: 10.24272/j.issn.2095-8137.2020.341
    Functional diversity is an integrative approach to better understand biodiversity across space and time. In the present study, we investigated the spatiotemporal patterns (i.e., elevation and season) and environmental determinants of anuran functional diversity on Tianping Mountain, northwest Hunan, China. Specifically, 10 transects were established from low (300 m a.s.l.) to high (1 492 m a.s.l.) elevations, and anuran communities were sampled in spring, early summer, midsummer, and autumn in 2017. Four functional diversity indices were computed for each transect in each season using ecomorphological functional traits. Our results demonstrated that these indices had contrasting responses to increasing elevations. However, they did not differ significantly among seasons in terms of temporal patterns. Interestingly, the unique spatiotemporal functional diversity patterns were impacted by distinct environmental variables, such as leaf litter cover, water temperature, number of trees, and water conductivity.
    MeSH terms: Altitude; Animals; Anura/classification*; Anura/physiology; Climate*; Biodiversity*; Animal Distribution*; Forests*
  17. Isotta E, Syafiq U, Ataollahi N, Chiappini A, Malerba C, Luong S, et al.
    Phys Chem Chem Phys, 2021 Jun 16;23(23):13148-13158.
    PMID: 34075978 DOI: 10.1039/d1cp01327k
    Cu-Zn disorder is known to deeply affect kesterite (Cu2ZnSnS4, CZTS) due to the low temperature order-disorder phase transition, leading to a random occupation of the two cations in the shared crystallographic planes. This defect complex has been extensively studied in the thin film photovoltaic sector, with considerable efforts in developing methods to quantify disorder. In this study, a preliminary investigation of thermoelectric properties in temperature for thin film CZTS is presented. It is found that Cu-Zn disorder enhances both electrical conductivity and Seebeck coefficient. This can positively affect the thermoelectric performance, showing a mechanism of potential interest for a broad class of quaternary chalcogenides. The order-disorder transition is clearly visible in the electronic properties. This feature is repeatable, with samples from different preparations and groups showing consistent results, qualitatively suggesting electronic measurements as possible methods to quantify disorder. Furthermore, the reversibility of the transition allows the electronic properties to be tuned via specific thermal treatments, pointing to interesting applications in tunable electronics.
  18. Assis RCP, Campos DR, Borges DA, Avelar BR, Pereira JASM, Matias CAR, et al.
    Rev Bras Parasitol Vet, 2021;30(2):e026020.
    PMID: 34076054 DOI: 10.1590/S1984-29612021012
    Platynosomum illiciens is a liver trematode encountered infecting mainly felids although it has also been reported in birds and in additional mammalian species, including non-human primates. The current study reports a natural P. illiciens infection primate of the genus Callithrix. The diagnosis was made using a combination of copro-parasitological techniques, morphological evaluation of adult specimens recovered from the liver during necropsy, and molecular analyses. Eggs were brown in color, oval, operculated, and contained a miracidium. Adult specimens recovered during necropsy were measured and showed dimensions compatible with P. illiciens. Molecular characterization of the trematode involved amplification by polymerase chain reaction (PCR), in combination with nucleotide sequencing, of an approximately 900 base pairs fragment corresponding to 18S-ITS1-5.8S ribosomal DNA. Sequenced amplicons showed 100% nucleotide identity with sequences deposited in the GenBank database as derived from specimens of P. illiciens recovered from cats in Malaysia and Brazil. It was concluded that the morphological and molecular analyses presented herein, confirmed the identification of the trematode recovered as P. illiciens.
    MeSH terms: Animals; Brazil; Callithrix; Cat Diseases*; Cats
  19. Munyati-Othman N, Appasamy SD, Damiri N, Emrizal R, Alipiah NM, Ramlan EI, et al.
    Curr Microbiol, 2021 Aug;78(8):2943-2955.
    PMID: 34076709 DOI: 10.1007/s00284-021-02550-5
    The glycine riboswitch is a known regulatory element that is unique in having two aptamers that are joined by a linker region. In this study, we investigated a glycine riboswitch located in the 5' untranslated region of a glycine cleavage system homolog (gcvTHP) in Burkholderia spp. Structure prediction using the sequence generated a model with a glycine binding pocket composed of base-triple interactions (G62-A64-A86 and G65-U84-C85) that are supported by A/G minor interactions (A17-C60-G88 and G16-C61-G87, respectively) and two ribose-zipper motifs (C11-G12 interacting with A248-A247 and C153-U154 interacting with A79-A78) which had not been previously reported. The capacity of the riboswitch to bind to glycine was experimentally validated by native gel assays and the crucial role of interactions that make up the glycine binding pocket were proven by mutations of A17U and G16C which resulted in conformational differences that may lead to dysfunction. Using glycine supplemented minimal media, we were able to prove that the expression of the gcvTHP genes found downstream of the riboswitch responded to the glycine concentrations introduced thus confirming the role of this highly conserved Burkholderia riboswitch and its associated genes as a putative glycine detoxification system in Burkholderia spp.
    MeSH terms: Glycine/genetics; Ligands; Nucleic Acid Conformation; Aptamers, Nucleotide*
  20. Tai CW, Gibbons K, Schibler A, Schlapbach LJ, Raman S
    J Nephrol, 2021 Jun 02.
    PMID: 34076880 DOI: 10.1007/s40620-021-01071-5
    BACKGROUND: Acute kidney injury (AKI) is a major cause of morbidity and mortality in critically ill children. The aim of this paper was to describe the prevalence and course of AKI in critically ill children and to compare different AKI classification criteria.

    METHODS: We conducted a retrospective observational study in our multi-disciplinary Pediatric Intensive Care Unit (ICU) from January 2015 to December 2018. All patients from birth to 16 years of age who were admitted to the pediatric ICU were included. The Kidney Disease Improving Global Outcomes (KDIGO) definition was considered as the reference standard. We compared the incidence data assessed by KDIGO, pediatric risk, injury, failure, loss of kidney function and end- stage renal disease (pRIFLE) and pediatric reference change value optimised for AKI (pROCK).

    RESULTS: Out of 7505 patients, 9.2% developed AKI by KDIGO criteria. The majority (59.8%) presented with stage 1 AKI. Recovery from AKI was observed in 70.4% of patients within 7 days from diagnosis. Both pRIFLE and pROCK were less sensitive compared to KDIGO criteria for the classification of AKI. Patients who met all three-KDIGO, pRIFLE and pROCK criteria had a high mortality rate (35.0%).

    CONCLUSION: Close to one in ten patients admitted to the pediatric ICU met AKI criteria according to KDIGO. In about 30% of patients, AKI persisted beyond 7 days. Follow-up of patients with persistent kidney function reduction at hospital discharge is needed to reveal the long-term morbidity due to AKI in the pediatric ICU.

    MeSH terms: Adolescent; Child; Follow-Up Studies; Hospitalization; Hospitals; Humans; Kidney; Patient Discharge; Reference Standards; Retrospective Studies; Intensive Care Units, Pediatric; Incidence; Prevalence; Critical Illness; Acute Kidney Injury
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