Browse publications by year: 2023

  1. Narapakdeesakul D, Pengsakul T, Kaewparuehaschai M, Thongsahuan S, Moonmake S, Lekcharoen P, et al.
    Acta Trop, 2023 Dec;248:107030.
    PMID: 37742788 DOI: 10.1016/j.actatropica.2023.107030
    Despite the natural occurrences of human infections by Plasmodium knowlesi, P. cynomolgi, P. inui, and P. fieldi in Thailand, investigating the prevalence and genetic diversity of the zoonotic simian malaria parasites in macaque populations has been limited to certain areas. To address this gap, a total of 560 long-tailed macaques (Macaca fascicularis) and 20 southern pig-tailed macaques (M. nemestrina) were captured from 15 locations across 10 provinces throughout Thailand between 2018 and 2021 for investigation of malaria, as were 15 human samples residing in two simian-malaria endemic provinces, namely Songkhla and Satun, who exhibited malaria-like symptoms. Using PCR techniques targeting the mitochondrial cytb and cox1 genes coupled with DNA sequencing, 40 long-tailed macaques inhabiting five locations had mono-infections with one of the three simian malaria species. Most of the positive cases of macaque were infected with P. inui (32/40), while infections with P. cynomolgi (6/40) and P. knowlesi (2/40) were less common and confined to specific macaque populations. Interestingly, all 15 human cases were mono-infected with P. knowlesi, with one of them residing in an area with two P. knowlesi-infected macaques. Nucleotide sequence analysis showed a high level of genetic diversity in P. inui, while P. cynomolgi and P. knowlesi displayed limited genetic diversity. Phylogenetic and haplotype network analyses revealed that P. inui in this study was closely related to simian and Anopheles isolates from Peninsular Malaysia, while P. cynomolgi clustered with simian and human isolates from Asian countries. P. knowlesi, which was found in both macaques and humans in this study, was closely related to isolates from macaques, humans, and An. hackeri in Peninsular Malaysia, suggesting a sylvatic transmission cycle extending across these endemic regions. This study highlights the current hotspots for zoonotic simian malaria and sheds light on the genetic characteristics of recent isolates in both macaques and human residents in Thailand.
    MeSH terms: Animals; Humans; Macaca fascicularis/parasitology; Malaysia/epidemiology; Parasites*; Phylogeny; Thailand/epidemiology
  2. Nguyen TH, Mat Daud ZA, Abd Talib R, Mohd R, Poh BK
    Transplant Proc, 2023 Nov;55(9):2176-2182.
    PMID: 37743189 DOI: 10.1016/j.transproceed.2023.08.008
    BACKGROUND: Although metabolic syndrome (MetS) is a significant risk factor, and dietary intake may affect the quality of life (QoL) of kidney transplant recipients (KTRs), the association between them are unclear. This study identified MetS and nutritional factors associated with the QoL of KTRs.

    METHODS: This study reports baseline data from a longitudinal study that was conducted at a hospital in Vietnam. KTRs aged ≥18 years and >3 months post-transplantation were recruited. Assessments included sociodemographic and blood biomarkers. Dietary intake was estimated from 24-hour recalls. A Short Form-36 Health Survey, comprising physical (PCS) and mental component summaries (MCS), was administered to assess QoL. Multivariate linear regression models were performed.

    RESULTS: The study included 106 patients (79 men) with a mean age of 43.2 years (± 11.9). Mean duration after kidney transplantation was 28.5 months (± 14.9). Patients with MetS had 6.43 lower PCS score (P < .05) and 3.20 lower MCS score (P < .05) than their counterparts without MetS. Calcium intake (β = -0.01; 95% CI, -0.03 to 0.00) and inadequate protein (β = -14.8; 95% CI, -23 to -6.65) were negatively associated with PCS score. MCS score was negatively associated with calcium intake (β = -0.02; 95% CI, -0.04 to -0.01) and inadequate protein intake (β = -15.1; 95% CI, -24.3 to -5.86), and positively associated with fat intake (β = 0.43, 95% CI, 0.02-0.85).

    CONCLUSIONS: MetS and poor dietary intake are independently associated with the QoL of KTRs. Nutritional intervention plans developed specifically for the recipients will improve dietary intake, reduce the incidence of MetS, and help enhance QoL.

    MeSH terms: Adolescent; Adult; Calcium; Eating; Humans; Longitudinal Studies; Male; Quality of Life; Transplant Recipients
  3. Rahman AAM, Islam MT, Moniruzzaman M, Rahim SKA, Singh M, Misran N, et al.
    Sci Rep, 2023 Sep 24;13(1):15943.
    PMID: 37743360 DOI: 10.1038/s41598-023-43182-y
    In this article, a unique metamaterial (MTM) structure is presented that exhibits four resonances of transmission coefficient (S21) that fall into S, X, and Ku bands. The MTM design is initiated on a Rogers (RT5880) substrate with an electrical dimension of 0.088 λ × 0.088 λ (λ is calculated at 3.424 GHz). The resonating patch contains four quartiles connected by a central metallic strip. The placement of each quartile is such that the whole resonator is mirror symmetric about the vertical axis. Two H-shaped modifiers connect two quartiles of each vertical half of the resonator. These H-shaped modifiers form the resonance cavity in its vicinity, and thus help significantly to orient the overall resonances of the proposed MTM at 3.424 GHz, 10 GHz, 14.816 GHz, and 16.848 GHz. The resonance phenomena are examined through equivalent circuit modeling and verified in Advanced Design Software (ADS). Metamaterial properties of the proposed MTM are extracted and it exhibits negative permittivity, permeability, and refractive index. The prototype of the MTM is fabricated and measurement is taken. The measured S21 shows a close similarity with the simulated result. Moreover, effective medium ratio (EMR) is calculated for the proposed MTM and a high EMR of 10.95 is obtained that expresses its compactness. This compact MTM with negative permittivity, permittivity, and refractive index can be important component for improving the performance of the miniaturized devices for multi-band wireless communication systems.
  4. Devi J, Chan TT, Lui R, Ali RAR, Women in GI Focus Group, Emerging Leaders Committee, Asian Pacific Association of Gastroenterology
    J Gastroenterol Hepatol, 2023 Dec;38(12):2047-2049.
    PMID: 37743674 DOI: 10.1111/jgh.16353
    MeSH terms: Asia/epidemiology; Gastroenterology*; Humans; Pacific Islands/epidemiology; Gastrointestinal Tract
  5. Wojciech L, Png CW, Koh EY, Kioh DYQ, Deng L, Wang Z, et al.
    EMBO J, 2023 Nov 02;42(21):e112963.
    PMID: 37743772 DOI: 10.15252/embj.2022112963
    The large intestine harbors microorganisms playing unique roles in host physiology. The beneficial or detrimental outcome of host-microbiome coexistence depends largely on the balance between regulators and responder intestinal CD4+ T cells. We found that ulcerative colitis-like changes in the large intestine after infection with the protist Blastocystis ST7 in a mouse model are associated with reduction of anti-inflammatory Treg cells and simultaneous expansion of pro-inflammatory Th17 responders. These alterations in CD4+ T cells depended on the tryptophan metabolite indole-3-acetaldehyde (I3AA) produced by this single-cell eukaryote. I3AA reduced the Treg subset in vivo and iTreg development in vitro by modifying their sensing of TGFβ, concomitantly affecting recognition of self-flora antigens by conventional CD4+ T cells. Parasite-derived I3AA also induces over-exuberant TCR signaling, manifested by increased CD69 expression and downregulation of co-inhibitor PD-1. We have thus identified a new mechanism dictating CD4+ fate decisions. The findings thus shine a new light on the ability of the protist microbiome and tryptophan metabolites, derived from them or other sources, to modulate the adaptive immune compartment, particularly in the context of gut inflammatory disorders.
    MeSH terms: Gastrointestinal Microbiome*; Animals; Tryptophan/metabolism; T-Lymphocytes, Regulatory; Mice; Eukaryota/metabolism; Microbiota*
  6. Vijayakumaran RK, Daly RM, Tan VPS
    Front Nutr, 2023;10:1176523.
    PMID: 37743924 DOI: 10.3389/fnut.2023.1176523
    This qualitative study is nested within a 12-week pilot randomized-controlled, two-arm trial involving high-intensity progressive resistance training (PRT) or PRT with a multi-nutrient, whey-protein supplementation (PRT+WP) in sarcopenic older adults (trial registration no: TCTR20230703001). The aim was to investigate sarcopenic participants' perceptions and barriers to this multi-modal intervention strategy that may accelerate "real-world" implementation. Eighteen older adults (one man) with possible sarcopenia were invited to join the study, of whom 16 women were randomized to a thrice-weekly PRT (n = 8) program (80% of 1-repetitive maximum, six resistance band exercises) only or PRT plus daily weekday milk-based WP supplementation (PRT+WP, n = 8). Muscle strength (handgrip and 5-times sit-to-stand), mass (dual-energy X-ray absorptiometry), performance (Short Physical Performance Battery and stair ascent-descent), and nutrition status (Mini Nutritional Assessment) were assessed for changes. We randomly selected eight women for the semi-structured interview. Post-intervention, eight (50%) women were sarcopenia-free, six (38%) remained in possible sarcopenia, one (6%) improved to sarcopenia, and one (6%) deteriorated from possible to severe sarcopenia. There were no significant between-group differences, but significant within-group improvements (p < 0.05) were detected for handgrip strength (PRT+WP 5.0 kg, d = 0.93; PRT 6.1 kg, d = 0.55), 5-times sit-to-stand time (PRT 2.0 s, d = 1.04), nutrition score (PRT+WP 3.44, d = 0.52; PRT 1.80, d = 0.44), and stair ascent time (PRT+WP 0.97 s, d = 0.77; PRT 0.75 s, d = 0.97). Our thematic analyses identified four main themes, namely, (1) perceived benefits, (2) sustaining behavior changes, (3) challenges in participating, and (4) improved wellbeing. Participants expressed how they initially were skeptical and doubted that they could complete the exercises or tolerate the milk-based WP supplements. However, they reported positive experiences and benefits felt from strength gains, increased confidence, and better physical abilities. Participants were surprised by the zero adverse effects of WP supplements. The women wanted more nutritional information and structured, guided exercise programs and suggested a community-based implementation. In conclusion, our findings showed PRT was well received and may support reduced risks of sarcopenia. No added benefits were seen with the addition of WP supplementation, but a larger sample is required to address this question. Overall, older (previously sarcopenic) Malay women indicated that they want more multi-modal programs embedded in their community.
  7. Aldalawi AA, Suardi N, Ahmed NM, Al-Farawn MAS, Dheyab MA, Jebur WI, et al.
    J Lasers Med Sci, 2023;14:e28.
    PMID: 37744010 DOI: 10.34172/jlms.2023.28
    Introduction: The depth of laser light penetration into tissue is a critical factor in determining the effectiveness of photodynamic therapy (PDT). However, the optimal laser light penetration depth necessary for achieving maximum therapeutic outcomes in PDT remains unclear. This study aimed to assess the effectiveness of laser light penetration depth at two specific wavelengths, 532 nm and 660 mm. Methods: Chicken and beef of different thicknesses (1, 3, 5, 10, and 20 mm±0.2 mm) were used as in vitro tissue models. The samples were subjected to irradiation by a low-level laser diode of 532 and 660 nm in continuous mode for 10 minutes. with power densities of 167 and 142 J/cm2, respectively. Laser light transmission through the tissue was measured using a power meter. Results: For beef samples, the 660 nm wavelength achieved a maximum transmission intensity of 30.7% at 1 cm thickness, while the 532 nm laser had a transmission intensity of 6.5%. Similarly, in chicken breast samples, the maximum transmission occurred at 1 cm thickness with 68.1% for the 660 nm wavelength and 18.2% for the 532 nm laser. Conclusion: Results consistently demonstrated a significant correlation (P<0.05) between tissue thickness and laser light penetration. Thicker tissues exhibited faster declines in light transmission intensity compared to thinner tissues within 10 minutes. These findings highlight the importance of further research to enhance light delivery in thicker tissues and improve the efficacy of PDT in various medical conditions.
  8. Krishna SS, Farhana SA, T P A, Hussain SM, Viswanad V, Nasr MH, et al.
    Front Immunol, 2023;14:1229667.
    PMID: 37744376 DOI: 10.3389/fimmu.2023.1229667
    The increasing prevalence of food allergies worldwide and the subsequent life-threatening anaphylactic reactions often have sparse treatment options, providing only symptomatic relief. Great strides have been made in research and in clinics in recent years to offer novel therapies for the treatment of allergic disorders. However, current allergen immunotherapy has its own shortcomings in terms of long-term efficacy and safety, due to the local side effects and the possibility of anaphylaxis. Allergen-specific immunotherapy is an established therapy in treating allergic asthma, allergic rhinitis, and allergic conjunctivitis. It acts through the downregulation of T cell, and IgE-mediated reactions, as well as desensitization, a process of food tolerance without any allergic events. This would result in a protective reaction that lasts for approximately 3 years, even after the withdrawal of therapy. Furthermore, allergen-specific immunotherapy also exploits several routes such as oral, sublingual, and epicutaneous immunotherapy. As the safety and efficacy of allergen immunotherapy are still under research, the exploration of newer routes such as intra-lymphatic immunotherapy would address unfulfilled needs. In addition, the existence of nanoparticles can be exploited immensely in allergen immunotherapy, which would lead to safer and efficacious therapy. This manuscript highlights a novel drug delivery method for allergen-specific immunotherapy that involves the administration of specific allergens to the patients in gradual increasing doses, to induce desensitization and tolerance, as well as emphasizing different routes of administration, mechanism, and the application of nanoparticles in allergen-specific immunotherapy.
    MeSH terms: Anaphylaxis*; Desensitization, Immunologic; Humans; Immune Tolerance; Immunity
  9. Duong KNC, Le LM, Veettil SK, Saidoung P, Wannaadisai W, Nelson RE, et al.
    Front Public Health, 2023;11:1206988.
    PMID: 37744476 DOI: 10.3389/fpubh.2023.1206988
    BACKGROUND: Meta-analyses have investigated associations between race and ethnicity and COVID-19 outcomes. However, there is uncertainty about these associations' existence, magnitude, and level of evidence. We, therefore, aimed to synthesize, quantify, and grade the strength of evidence of race and ethnicity and COVID-19 outcomes in the US.

    METHODS: In this umbrella review, we searched four databases (Pubmed, Embase, the Cochrane Database of Systematic Reviews, and Epistemonikos) from database inception to April 2022. The methodological quality of each meta-analysis was assessed using the Assessment of Multiple Systematic Reviews, version 2 (AMSTAR-2). The strength of evidence of the associations between race and ethnicity with outcomes was ranked according to established criteria as convincing, highly suggestive, suggestive, weak, or non-significant. The study protocol was registered with PROSPERO, CRD42022336805.

    RESULTS: Of 880 records screened, we selected seven meta-analyses for evidence synthesis, with 42 associations examined. Overall, 10 of 42 associations were statistically significant (p ≤ 0.05). Two associations were highly suggestive, two were suggestive, and two were weak, whereas the remaining 32 associations were non-significant. The risk of COVID-19 infection was higher in Black individuals compared to White individuals (risk ratio, 2.08, 95% Confidence Interval (CI), 1.60-2.71), which was supported by highly suggestive evidence; with the conservative estimates from the sensitivity analyses, this association remained suggestive. Among those infected with COVID-19, Hispanic individuals had a higher risk of COVID-19 hospitalization than non-Hispanic White individuals (odds ratio, 2.08, 95% CI, 1.60-2.70) with highly suggestive evidence which remained after sensitivity analyses.

    CONCLUSION: Individuals of Black and Hispanic groups had a higher risk of COVID-19 infection and hospitalization compared to their White counterparts. These associations of race and ethnicity and COVID-19 outcomes existed more obviously in the pre-hospitalization stage. More consideration should be given in this stage for addressing health inequity.

    MeSH terms: African Americans/statistics & numerical data; Ethnic Groups/statistics & numerical data; Hispanic Americans/statistics & numerical data; Hospitalization/statistics & numerical data; Humans; United States/epidemiology; Vaccination; Outcome Assessment (Health Care)/statistics & numerical data
  10. Muhamad N, Amali NAN
    Iran J Public Health, 2023 Aug;52(8):1565-1577.
    PMID: 37744537 DOI: 10.18502/ijph.v52i8.13396
    BACKGROUND: Digital devices such as smartphones, tablets, computers, and laptops are used for various purposes. The digital display quality has been improved, making it less tiring and more favoured among users. This study aimed to review the visual comfort of digital devices and the preferences of digital display settings that enhance the visual comfort experienced by digital device users.

    METHODS: A search of PubMed, EBSCO host MEDLINE Complete, Scopus database, Google Scholar, and manual citation review was conducted, covering the period between 2010 and 2022. The criteria were selected based on the PRISMA statements. The search mainly focused on finding the existing literature on digital devices that contribute to visual discomfort and digital device settings that provide better visual comfort.

    RESULTS: The database search resulted in 533 references via the application of Microsoft Excel. There were 28 studies included in the final assessment. Twelve studies accounted for digital devices that contributed to visual discomfort, while another sixteen studies for digital device settings provided better visual comfort.

    CONCLUSION: Digital displays with high luminance contrast, positive polarity and adequate colour were preferred for better visual comfort. Meanwhile, smaller fonts were preferred for desktops and laptops, while larger fonts were favoured for smartphones. This study provides insights for digital display developers to learn and improve their display technology to fit the preferences expressed.

  11. Foley L
    Asian Pac Migr J, 2023 Jun;32(2):208-233.
    PMID: 37744613 DOI: 10.1177/01171968231189139
    This paper analyzes how policy-relevant actors understand the causes and effects of labor immigration to Malaysia, the country that receives the highest number of migrant workers in Southeast Asia. Whereas most research on international migration governance has focused on governance system outputs, this paper adopts an actor-centered perspective to investigate how actors narratively construct labor migration dynamics in Malaysia and how they conceptualize the drivers and impacts of labor migration policies and practices. The empirical material comes from 41 in-depth interviews with government officials, policymakers, international and regional organizations, nongovernmental organizations, employers' organizations, trade unions, and embassy representatives. The study found that Malaysia's migration governance system was perceived as "chaotic" due to the seemingly inconsistent, unclear "ad hoc" policy measures implemented, and that the governance system is perceived as "corrupt." Economic incentives were also seen as the primary driver of labor immigration, yet the main impact on Malaysian society was perceived as the spread of criminality, violence and disease, a narrative centered on migrant men. This paper argues that this discourse is problematic as it may drive types of policy measures that target migrant men.
  12. Lim T, Kerscher M, Ogilvie A, Pavicic T, Lorenc PZ, Frank K, et al.
    Plast Reconstr Surg Glob Open, 2023 Sep;11(9):e5287.
    PMID: 37744770 DOI: 10.1097/GOX.0000000000005287
    BACKGROUND: The objective of this investigation was to create and validate five-point photonumeric scales which assess static and dynamic forehead lines.

    METHODS: Two different novel five-point photonumeric scales for the assessment of static and dynamic forehead lines were developed. Moreover, a photoguide was created, including subjects from both sexes, all age groups, and different Fitzpatrick skin types. A total of 11 raters from all over the world were involved in the digital validation, whereas four raters performed a live validation.

    RESULTS: The Croma Static Forehead Lines-Assessment Scale showed almost perfect inter and intra-rater agreement in both the digital and the live setting with inter-rater intraclass correlation coefficients of 0.86 [95% confidence interval (CI): 0.82-0.89] in the first digital rating and 0.82 [95% CI: 0.78-0.86] in the second digital rating. The Croma Dynamic Forehead Lines-Assessment Scale showed almost perfect inter and intra-rater agreement in the digital setting with inter-rater intraclass correlation coefficients of 0.83 [95% CI: 0.79-0.86] in the first digital rating and 0.80 [95% CI: 0.75-0.84] in the second rating and almost substantial agreement in the live setting.

    CONCLUSIONS: The Croma Static Forehead Lines-Assessment Scale and the Croma Dynamic Forehead Lines-Assessment Scale have excellent inter and intra-rater agreements to be justifiably used in the clinical and study setting, both digitally and live across ethnic groups.

  13. Tung XY, Yip JQ, Gew LT
    ACS Omega, 2023 Sep 19;8(37):33115-33201.
    PMID: 37744793 DOI: 10.1021/acsomega.3c02994
    Excessive UV radiation (UVR) exposure has been shown in studies to be a major risk factor for most melanomas, causing premature skin aging as well as immune system suppression due to the increased production of hydrogen peroxide (H2O2) and other reactive oxygen species (ROS) during the melanogenesis process. Although several compounds with antioxidant and antityrosinase activities are widely used in the cosmetic industry, like kojic acid, hydroquinone, ascorbic acid, and arbutin, their use has been limited due to their adverse effects on the skin and cytotoxic issues. Recently, attempts have been made to develop new natural skin-lightening products by using plant extracts that are less toxic and exhibit numerous biological properties with great market demand. In this study, information on the depigmentation effects of various natural plant species was gathered from the SCOPUS database according to the PRISMA guidelines. A total of 414 records were retrieved, and finally, 76 articles were included in the qualitative synthesis by fulfilling all the inclusion criteria. In this review, we discuss the extraction methods and biological assays of 75 highly potential plant species, including the olive, yuzu, longan, and lotus. We concluded that the use of natural plants as skin-whitening agents is highly effective as there is a significant correlation between the content of polyphenolic compounds, antimelanogenesis, antityrosinase, and antioxidant activities. However, it is worth noting that the use of extraction methods or types of solvents is very important in determining the biological activities of plants.
  14. Madinah R, Rusydi F, Fadilla RN, Khoirunisa V, Boli LSP, Saputro AG, et al.
    ACS Omega, 2023 Sep 19;8(37):34022-34033.
    PMID: 37744805 DOI: 10.1021/acsomega.3c04907
    Noncovalent interactions, such as dispersion, play a significant role in the stability of flexible molecules, such as curcumin. This study revealed the importance of dispersion correction in the structure and keto-enol tautomerization of curcumin, which has rarely been addressed in computational studies. We rigorously constructed all possible unique curcumin conformers in the enol and keto forms within the first-principles framework. Regardless of the different environments, we carefully explained the agreement between the computational geometry (in the gas phase) and the experimental measurement (in the polymorph) by using dispersion correction. The calculation results for the aqueous solution of conformational abundance, thermochemistry, and reaction kinetics support the experimental observations after considering the dispersion correction. The study also suggests a water-catalyzed mechanism for keto-enol tautomerization, where dispersion correction plays a role in decreasing the energy barrier and making the keto form thermochemically and kinetically favorable. Our results could be helpful in future computational studies to find a method for increasing the aqueous solubility of curcumin; hence, the potential of curcumin as a multifunctional medicine can be fully achieved.
  15. Rahman RA, Masrom S, Mohamad M, Sari EN, Saragih F, Rahman ASA
    MethodsX, 2023 Dec;11:102364.
    PMID: 37744883 DOI: 10.1016/j.mex.2023.102364
    Machine learning has been very promising in solving real problems, but the implementation involved difficulties mainly for the inexpert data scientists. Therefore, this paper presents an automated machine learning (AutoML) to simplify and accelerate the modeling tasks. Focused on Python and RapidMiner rapid modeling tools, Tree-based Pipeline Optimization Tool (TPOT) and AutoModel were used. This paper presents a comprehensive comparison between these tools with regard to the prediction accuracy and Area Under Curve (AUC) in classifying real cases of whistleblowing academic dishonesty among undergraduate students of two universities in Indonesia. Additionally, the correlations weight from demographic and Theory of Planned Behavior (TOB) attributes in the different machine learning models are also discussed. All the machine learning algorithms from TPOT and AutoModel are considerable powerful to generate good accuracy level (between 70-93% of AUC) in classifying both cases of whistleblowing and non-whistleblowing on the hold-out samples from the testing process. Generally, based on the validation results of the prediction models, demographic attributes presented more importance than the TBP attributes. The findings of this study will be a great interest of many research scholars to conduct a more in-depth analysis on AutoML for many domains mainly in education and academic misconduct fields.•AutoML is the first of its kind to be empirically compared between TPOT and AutoModel in an application to predict academic dishonesty whistleblowing.•Besides accuracy performances of the AutoML, the proportion of the variance of each attribute from demographic and Theory of Planned Behavior (TPB) is also presented in the prediction models of academic dishonesty whistleblowing.•AutoML is a convenient and reproducible rapid modeling method of machine learning to be used in many kinds of prediction problem.
  16. Lai NHY, Mohd Zahir IA, Liew AKY, Ogawa S, Parhar I, Soga T
    Front Behav Neurosci, 2023;17:1205175.
    PMID: 37744951 DOI: 10.3389/fnbeh.2023.1205175
    Stress is an important aspect of our everyday life and exposure to it is an unavoidable occurrence. In humans, this can come in the form of social stress or physical stress from an injury. Studies in animal models have helped researchers to understand the body's adaptive response to stress in human. Notably, the use of behavioural tests in animal models plays a pivotal role in understanding the neural, endocrine and behavioural changes induced by social stress. Under socially stressed conditions, behavioural parameters are often measured physiological and molecular parameters as changes in behaviour are direct responses to stress and are easily assessed by behavioural tests. Throughout the past few decades, the rodent model has been used as a well-established animal model for stress and behavioural changes. Recently, more attention has been drawn towards using fish as an animal model. Common fish models such as zebrafish, medaka, and African cichlids have the advantage of a higher rate of reproduction, easier handling techniques, sociability and most importantly, share evolutionary conserved genetic make-up, neural circuitry, neuropeptide molecular structure and function with mammalian species. In fact, some fish species exhibit a clear diurnal or seasonal rhythmicity in their stress response, similar to humans, as opposed to rodents. Various social stress models have been established in fish including but not limited to chronic social defeat stress, social stress avoidance, and social stress-related decision-making. The huge variety of behavioural patterns in teleost also aids in the study of more behavioural phenotypes than the mammalian species. In this review, we focus on the use of fish models as alternative models to study the effects of stress on different types of behaviours. Finally, fish behavioural tests against the typical mammalian model-based behavioural test are compared and discussed for their viability.
  17. Kwan MSK, Tan SL, Keowmani T, Goh SL, Voo JYH, Tan HS, et al.
    Asia Pac Allergy, 2023 Sep;13(3):121-126.
    PMID: 37744958 DOI: 10.5415/apallergy.0000000000000115
    BACKGROUND: Given the deficits in allergists and testing capacity, the diagnosis of drug allergy is largely dependent on the clinician's and pharmacist's judgment. The ability to recognize drug allergies and respond appropriately is crucial to patient safety. Currently, there is a void in the evidence that limits the ability to recommend comprehensive and swift improvements on this front.

    OBJECTIVE: This study thus aimed to evaluate the knowledge, attitude, and practice toward drug allergy among doctors and pharmacists working in public healthcare facilities in Sabah, Malaysia.

    METHODS: This cross-sectional study was conducted in 24 hospitals and 11 clinics in Sabah. A validated Drug Allergy Knowledge, Attitude, and Practice Questionnaire was adapted from a published study and developed on an online survey platform. The questionnaire was distributed to all listed eligible respondents via email and personal messenger service.

    RESULTS: A total of 549 doctors and pharmacists responded, with an overall response rate of 18.2%. The total mean knowledge, attitude, and practice scores were 8.3 (SD, 1.98), 18.9 (SD, 2.55), and 17.3 (SD, 4.4), respectively. It was found that pharmacists performed significantly poorer than both medical officers (mean score difference = -0.5; P = 0.006) and specialists (mean score difference = -0.9; P = 0.020) in the knowledge domain. As the time in service doubles, the knowledge score increases significantly by 0.3 (P = 0.015).

    CONCLUSION: Knowledge, attitude, and practice on drug allergy among doctors and pharmacists in Sabah were poor. It is thus timely for advanced educational programs on drug allergy to be formalized and implemented.

  18. Thong BY, Pawankar R, Park HS, Abdul Latiff AH
    Asia Pac Allergy, 2023 Sep;13(3):127-131.
    PMID: 37744960 DOI: 10.5415/apallergy.0000000000000114
    Streptococcus pneumoniae (pneumococcus) is a significant cause of bacterial infections ranging from mild infections affecting the respiratory tract such as otitis media and sinusitis to severe diseases including bacteremia, pneumonia, and invasive pneumococcal disease (IPD) (eg, meningitis, septic arthritis, and endocarditis). Pneumococcal vaccines were first developed in the 1970s as capsular pneumococcal polysaccharide vaccines, which were T-cell independent and hence lacked immunologic memory. Subsequently in the year 2000, pneumococcal conjugate vaccines (PCV) conjugated to a protein to increase immunogenicity were developed and made commercially available. The increasing number of pneumococcal serotypes identified and the expanding pipeline of PCV vaccines with improved immunogenicity have significantly reduced the morbidity and mortality associated with IPD in high-risk patients. Pneumococcal vaccines also play an important role in the diagnosis and immunophenotyping of children and adults with inborn errors of immunity (IEI) given the increasing diversity/heterogeneity of IEI presenting with primary and/or specific antibody deficiency. Other than the quantitation of serotype levels in routine clinical care, other measurements of immune response including the functional activity of antibodies, antibody avidity, cell-mediated immunity, and immunological memory remain limited to clinical trials during vaccine development.
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