Browse publications by year: 2023

Fulltext Protein Hydrolysate from Underutilized Legumes: Unleashing the Potential for Future Functional Foods

Azman AT, Mohd Isa NS, Mohd Zin Z, Abdullah MAA, Aidat O, Zainol MK

Prev Nutr Food Sci, 2023 Sep 30;28(3):209-223.
PMID: 37842256 DOI: 10.3746/pnf.2023.28.3.209

Proteins play a vital role in human development, growth, and overall health. Traditionally, animal-derived proteins were considered the primary source of dietary protein. However, in recent years, there has been a remarkable shift in dietary consumption patterns, with a growing preference for plant-based protein sources. This shift has resulted in a significant increase in the production of plant proteins in the food sector. Consequently, there has been a surge in research exploring various plant sources, particularly wild, and underutilized legumes such as Canavalia, Psophocarpus, Cajanus, Lablab, Phaseolus, and Vigna, due to their exceptional nutraceutical value. This review presents the latest insights into innovative approaches used to extract proteins from underutilized legumes. Furthermore, it highlights the purification of protein hydrolysate using Fast Protein Liquid Chromatography. This review also covers the characterization of purified peptides, including their molecular weight, amino acid composition, and the creation of three-dimensional models based on amino acid sequences. The potential of underutilized legume protein hydrolysates as functional ingredients in the food industry is a key focus of this review. By incorporating these protein sources into food production, we can foster sustainable and healthy practices while minimizing environmental impact. The investigation of underutilized legumes offers exciting possibilities for future research and development in this area, further enhancing the utilization of plant-based protein sources.
Fulltext Dietary habits and physical activity patterns in relation to nutritional status among school-aged children in Pakistan: A cross-sectional study

Qureshi S, Iqbal M, Rafiq A, Ahmed H, Malik T, Kalam MN, et al.

AIMS Public Health, 2023;10(3):553-567.
PMID: 37842276 DOI: 10.3934/publichealth.2023039

BACKGROUND: Childhood malnutrition remains a significant public health problem impacting the physical and mental growth if school aged children, particularly in limited-resource countries.
OBJECTIVE: The study objective was to assess levels of physical activity, patterns of screen time (S.T.), the relationship between physical activity and screen time patterns, and how these factors affect growth status (adjusting for socioeconomic status).
METHODOLOGY: A cross-sectional study included 3,834 children between 6-14 years attending pre-selected schools. Teachers, students, and parents were invited to fill out a standardized questionnaire, and Body Mass Index (BMI) was calculated using Center for disease control (CDC) centile charts. A Chi-square was performed to see the possible association between any height and weight abnormalities and all possible risk factors. Multivariate logistic regression was applied to see the effect of variables significantly associated with univariate analysis.
RESULTS: Approximately 2,447 (63.8%) children were between 11-14 years old and 1,387 (36.2%) were between 4-10 years old. The mean height was 143.71 ± 16.51 centimetres, the mean weight was 36.5 ± 12.9 kilogram, and the mean BMI was 17.16 ± 3.52. Multivariate logistic regression status and junk food combined affected stunting socioeconomic status was significantly associated with being underweight p = 0.001.
CONCLUSION: Childhood obesity and stunting remain significant problems in Pakistani school-going children. These are significantly associated with poverty, a lack of physical activity opportunities, and available food quality.
Fulltext Exploring barriers to accessing healthcare services for older indigenous people in the Chittagong Hill Tract, Bangladesh

Hossen MS, Sohel MS, Horaira GA, Laskor MAH, Sumi ABA, Chowdhury S, et al.

AIMS Public Health, 2023;10(3):678-697.
PMID: 37842279 DOI: 10.3934/publichealth.2023047

We aim to investigate the obstacles faced by elderly indigenous individuals in the Chittagong Hill Tracts, Bangladesh when accessing healthcare services. A qualitative research approach was utilized, and data collection was carried out in three distinct regions of the aforementioned area. A total of 30 in-depth, semi-structured interviews and participant observations were conducted to achieve the research objectives. Thematic analysis utilizing both a deductive and inductive approach was employed to analyze the data. The Granheim method and Nvivo-12 software were utilized to process, analyze and code the data. The study's findings indicate that a lack of knowledge about healthcare needs, geographical barriers, poor financial conditions, higher cost of medical services, scarcity of hospitals nearby and communication barriers all contribute to inadequate access to healthcare services. By recognizing the factors that impede access to healthcare services in this region, this study offers valuable insight for policymakers and healthcare providers on how to enhance healthcare services for the indigenous population, especially the elderly. Furthermore, the government can adopt a more efficient approach to include these elderly individuals in various social safety net programs.
Fulltext Scaphoid Plate Osteosynthesis in Complex Fractures and Wrist Trauma: A Case Series

Liau CJ, Liew SK, Arsad SR, Muhammad Nawawi RF, Silvanathan JP

Cureus, 2023 Sep;15(9):e45067.
PMID: 37842372 DOI: 10.7759/cureus.45067

Introduction Treatment of scaphoid fracture is challenging due to its unique blood supply and geometry. Traditionally, a headless compression screw is the standard treatment for unstable scaphoid fracture. Some fractures are complex, for example, comminution with bone loss. A scaphoid plate is an option in these difficult fractures providing adequate rotational stability. Aim To share our experience in using scaphoid plates in complex wrist trauma and comminuted fractures. Method and material Complex wrist trauma involving scaphoid fractures that were comminuted and multi-fragmentary fractures treated with plate osteosynthesis were retrospectively reviewed between July 2019 and September 2021. Patient demographic data, preoperative radiographs, CT scans, pain, wrist range of motion, and fracture union rate to union were reviewed. Quick Disabilities of the Arm, Shoulder, and Hand (QuickDASH®) score was recorded at 1-year postoperative follow-up. Results Nine patients associated with complex wrist trauma were included in this case series. The mean follow-up was 2.5 years (13-30 months). The union rate was 100%. The mean arc of motion was 105° (95-110°). QuickDASH® score was 19.96 at 1-year postoperative follow-up. Four patients had good outcomes, four satisfactory, and one poor outcome. One hardware complication was observed which was the impingement of the plate proximally over the articular surface of the distal radius. Conclusion A scaphoid plate is a reliable option for treating complex and difficult fractures. It provides adequate stability, especially in comminution, bone loss, or multi-fragmentary fractures which are not amendable using other fixation methods. We recommend the expansion of plate osteosynthesis beyond scaphoid nonunion into complex wrist trauma.
Fulltext A new surgical modality for breast reconstruction in patients with breast cancer: a case report and literature review

Liu J, He G, Zhang H, Yahya MM

Gland Surg, 2023 Sep 25;12(9):1318-1325.
PMID: 37842525 DOI: 10.21037/gs-23-5

BACKGROUND: Breast preservation can considerably improve the postoperative living quality of breast cancer patients. Through this study, we proposed a novel, safe, and effective surgical modality for breast preservation in patients with early breast cancer.
CASE DESCRIPTION: Herein, we present a case report of a patient with early-stage breast cancer (T1cN0M0), who underwent skin-sparing nipple areola hypodermic gland resection combined with primary breast reconstruction using silicone implants. The patient was administered with general anesthesia, and the implants were inserted using a insufflation-free suspension and hook suspension under the pectoralis major muscle. The patient was followed up on postoperative days 1, 2, 7, 14, 30, 60, and 100 to check for any complications, such as upper limb edema, paresthesia, or flap necrosis. The patient experienced no complications. No obvious surgical scars or axillary surface infections were observed. The patient was satisfied with the surgical outcome, and this treatment approach reduced her treatment costs by approximately USD 2,600.
CONCLUSIONS: The new surgical procedure for breast reconstruction considerably improved the quality of life of the patient; no postoperative complications such as skin flap necrosis, paresthesia, or upper limb edema were experienced by the patient; and the treatment costs were reduced. In addition, this method effectively overcomes the concerns related to axillary space instability and limited operative space, rendering it worthy of promotion in clinical practice.
Fulltext Influence of urban forests on residential property values: A systematic review of remote sensing-based studies

Ewane EB, Bajaj S, Velasquez-Camacho L, Srinivasan S, Maeng J, Singla A, et al.

Heliyon, 2023 Oct;9(10):e20408.
PMID: 37842597 DOI: 10.1016/j.heliyon.2023.e20408

Urban forests provide direct and indirect benefits to human well-being that are increasingly captured in residential property values. Remote Sensing (RS) can be used to measure a wide range of forest and vegetation parameters that allows for a more detailed and better understanding of their specific influences on housing prices. Herein, through a systematic literature review approach, we reviewed 89 papers (from 2010 to 2022) from 21 different countries that used RS data to quantify vegetation indices, forest and tree parameters of urban forests and estimated their influence on residential property values. The main aim of this study was to understand and provide insights into how urban forests influence residential property values based on RS studies. Although more studies were conducted in developed (n = 55, 61.7%) than developing countries (n = 34, 38.3%), the results indicated for the most part that increasing tree canopy cover on property and neighborhood level, forest size, type, greenness, and proximity to urban forests increased housing prices. RS studies benefited from spatially explicit repetitive data that offer superior efficiency to quantify vegetation, forest, and tree parameters of urban forests over large areas and longer periods compared to studies that used field inventory data. Through this work, we identify and underscore that urban forest benefits outweigh management costs and have a mostly positive influence on housing prices. Thus, we encourage further discussions about prioritizing reforestation and conservation of urban forests during the urban planning of cities and suburbs, which could support UN Sustainable Development Goals (SDGs) and urban policy reforms.
Fulltext Predictive analysis of the power spectral irradiance from blackbody radiation source using single pixel detector

Lee HJ, Ker PJ, Gamel MMA, Jamaludin MZ, Wong YH

Heliyon, 2023 Oct;9(10):e20585.
PMID: 37842600 DOI: 10.1016/j.heliyon.2023.e20585

Accurate spectral irradiance measurement in the near-infrared range is significant for the design and characterization of photodetector and photovoltaic cells. Approximation method is commonly used to solve for the input power using estimated spectral irradiance, where the dependency on wavelength and temperature remains uncertain. This study aims to determine the power spectrum at different radiation temperatures using a single pixel photodetector, taking into consideration factors such as transmission spectra of alumina radiator, CaF2 collimating lens, responsivity, and measured photocurrent information of photodetectors. Utilizing predictive mathematical model, five commercial photodetectors, including Silicon, Germanium, In0.53Ga0.47As, In0.73Ga0.27As, and In0.83Ga0.17As were used to solve for the power densities as a function of wavelengths at radiation temperatures of 1000 °C and 1500 °C. The spectral irradiance of photodetectors was determined with a percentage difference of <4.9 %, presenting an accurate power density estimation for the spectrum at a wide range of radiation temperatures. Power irradiance data obtained were validated in the narrow wavelength range with 1000 nm, 1400 nm, 1500 nm, and 2000 nm bandpass filters. The reported work demonstrates a simple and efficient way which could contribute to develop a cost-effective method of measuring and determining the spectrum irradiances of objects at different radiation temperatures. This predictive analysis method hopefully intensifies the progress of efforts to reduce the reliance on complex optoelectronic instruments in accurately solving power irradiance information.
Fulltext Examining the critical factors of internal audit effectiveness from internal auditors' perspective: Moderating role of extrinsic rewards

Alqudah H, Amran NA, Hassan H, Lutfi A, Alessa N, Alrawad M, et al.

Heliyon, 2023 Oct;9(10):e20497.
PMID: 37842607 DOI: 10.1016/j.heliyon.2023.e20497

An effective internal auditor can support an organization in achieving its goals and protect its assets and funds. However, to be effective, the internal auditors need to be empowered with relevant resources. This study aims at probing the influence of the empowerments (management support, external auditors' collaboration, independence, size of internal audit units, and extrinsic rewards) on the internal auditors' effectiveness, and to examin whether extrinsic rewards moderate the association between respective empowerments and the effectiveness of Jordanian public sector internal auditors'. The current work relied on 117 surveys collected from Jordan's internal audit and financial managers in the public sectors. According to the Resource-Based View (RBV), the findings show that management support, external auditors cooperation, independence, and extrinsic rewards all have a significant influence on the effectiveness of internal auditor. The of the Internal Audit Department (IAD) size was discovered to be insignificant. Also, the results have revealed partial support for the influence of the extrinsic reward as a moderator. Because of the prominence of the public sector in the Jordanian market, this current research expands on the idea of enabling internal auditor (IA) to accomplish their assigned objectives. The findings might help authorities develop new norms and legislation for the internal audit profession. These empowering characteristics may also improve internal auditors' capacity to execute their duty in saving public monies and limiting corrupt practices inside public sector organizations.
Fulltext Broad vs. narrow traits: a scoping review of measuring personality traits in teacher selection using the situational judgment test

Nadmilail AI, Mohd Matore MEE, Maat SM, Sheridan L

Front Psychol, 2023;14:1217321.
PMID: 37842695 DOI: 10.3389/fpsyg.2023.1217321

Situational Judgment Tests (SJTs) have gained popularity and are commonly used as a measurement technique in a variety of professions, particularly those that include hiring, promoting, and professional development. In various educational sectors around the world, SJTs are being utilized as a measure by which to choose individuals who possess the requisite non-academic attributes for the profession. The objective of this review is to identify and analyze the traits that are measured in teacher selection using SJTs, in terms of both broad and narrow traits. This review uses a scoping review approach comprising five stages which are: identifying the research question, identifying relevant studies, selecting the studies, charting the data and reporting the results. Seven empirical research studies on teacher selection using SJTs were identified in which broad and narrow traits are used differently in selection. In the studies, a broad trait-conscientiousness-and seven narrow traits-organization and planning; empathy and communication; adaptability and resilience; mindset; emotional regulation; professional ethics; and enthusiasm and motivation-were recognized. Analysis revealed, in line with other prior studies, that the traits discovered tended to be used as the foundation for teacher selection criteria. The use of broad and narrow traits as the basis for teacher selection criteria has implications for the selection of the "best" teacher candidates because both broad and narrow traits generally do not accurately measure the precise characteristics needed. Future studies should focus on measuring more precise characteristics without overlap between the targeted characteristics, in light of the conclusions from this review.
Improving the diagnosis of endometriosis in Asia-Pacific: Consensus from the Asia-Pacific Endometriosis Expert Panel for Endometriosis

Yen CF, Hamdan M, Hengrasmee P, Huang Z, Jeong K, Dao LA, et al.

Int J Gynaecol Obstet, 2023 Dec;163(3):720-732.
PMID: 37837343 DOI: 10.1002/ijgo.15142

Endometriosis should be diagnosed as early as possible in the continuum of care; but substantial delays of approximately 6-8 years between symptom onset and endometriosis diagnosis have been widely reported. With the purpose of improving the prompt diagnosis of endometriosis, the Asia-Pacific Endometriosis Expert Panel (APEX) sought to address the reasons for diagnostic delays across the region, and formulate a multi-pronged approach to overcoming these challenges. In the first instance, clinical diagnosis is preferable to surgical diagnosis, in order to facilitate earlier empirical treatment and minimize the negative sequelae of undiagnosed/untreated disease. There should be a high clinical index of suspicion in women presenting with cyclical symptoms, including those involving extrapelvic organs. Diagnostic delays in Asia-Pacific countries are attributable to a variety of patient, physician, and healthcare factors, including poor awareness, normalization/trivialization of pain, individual/cultural attitudes toward menstruation, default use of symptom-suppressing treatments, misdiagnosis, and a lack of diagnostic resourcing or adequate referral pathways in some areas. Suggested initiatives to reduce diagnostic delays are geared toward improving public awareness, improving clinical diagnostic skills, streamlining multidisciplinary care pathways for timely referral, updating and implementing diagnostic guidelines, lobbying policymakers and insurance companies for endometriosis support, and increasing efforts to bridge data gaps and perform further research in this field. Formulating specific action plans and gathering traction are the responsibility of individual countries within local parameters. The APEX group advocates for any initiatives and policies that support the unmet needs of women with endometriosis, to improve patient experience and outcomes.

MeSH terms: Asia; Female; Humans; Menstruation; Pain; Consensus
Fulltext Butylparaben weakens female fertility via causing oocyte meiotic arrest and fertilization failure in mice

Zhang Y, Sun L, Zhang D, Gao Y, Ma H, Xue Y, et al.

Ecotoxicol Environ Saf, 2023 Nov 01;266:115561.
PMID: 37837697 DOI: 10.1016/j.ecoenv.2023.115561

Butylparaben is an ubiquitous environmental endocrine disruptor, that is commonly used in cosmetics and personal care product due to its anti-microbial properties. Butylparaben has been shown to cause developmental toxicity, endocrine and metabolic disorders and immune diseases. However, little is known about the impact on female fertility, especially oocyte quality. In the present study, we reported that butylparaben influenced female fertility by showing the disturbed oocyte meiotic capacity and fertilization potential. Specifically, butylparaben results in the oocyte maturation arrest by impairing spindle/chromosome structure and microtubule stability. Besides, butylparaben results in fertilization failure by impairing the dynamics of Juno and ovastacin and the sperm binding ability. Last, single-cell transcriptome analysis showed that butylparaben-induced oocyte deterioration was caused by mitochondrial dysfunction, which led to the accumulation of ROS and occurrence of apoptosis. Collectively, our study indicates that mitochondrial dysfunction and redox perturbation is the major cause of the weakened female fertility expoesd to butylparaben.

MeSH terms: Animals; Female; Fertility; Fertilization; Male; Meiosis*; Oocytes/metabolism; Semen*; Mice
Fulltext Dalbergiella welwitschia (Baker) Baker f. alkaloid-rich extracts attenuate liver damage in streptozotocin-induced diabetic rats

Ajiboye BO, Dada S, Fatoba HO, Lawal OE, Oyeniran OH, Adetuyi OY, et al.

Biomed Pharmacother, 2023 Dec;168:115681.
PMID: 37837880 DOI: 10.1016/j.biopha.2023.115681

This experiment was conducted to evaluate the Dalbergiella welwitschia alkaloid-rich extracts on liver damage in streptozotocin-induced diabetic rats. Hence, to induce diabetes, 45 mg/kg body weight of streptozotocin was intraperitoneally injected into the Wistar rats. Subsequently, 5 % (w/v) of glucose water was given to the induced animals for 24 h. Thus, the animals (48) were grouped into five groups (n = 8), containing normal control (NC), diabetic control (DC), diabetic rats placed on low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of D. welwitschi alkaloid-rich leaf extracts (i.e. DWL and DWH respectively), and diabetic rats administered 200 mg/kg body weight of metformin (MET). The animals were sacrificed on the 21st day of the experiment, blood and liver were harvested, and different liver damage biomarkers were evaluated. The results obtained demonstrated that diabetic rats administered DWL, DWH and MET significantly (p 0.05) different when compared with NC. Also, diabetic rats administered DWL, DWH and MET revealed a significant (p 0.05) different when compared with NC. In addition, histological examination revealed that diabetic rats placed on DWL, DWH and MET normalized the hepatocytes. Consequently, it can be inferred that alkaloid-rich extracts from D. welwitschi leaf could be helpful in improving liver damage associated with diabetes mellitus rats.

MeSH terms: Animals; Blood Glucose/metabolism; Body Weight; Hypoglycemic Agents/adverse effects; Liver/metabolism; Plant Extracts/adverse effects; Streptozocin/adverse effects; Rats, Wistar; Oxidative Stress; Rats
Eminent destruction of organics and pathogens concomitant with power generation in a visible light-responsive photocatalytic fuel cell with NiFe2O4/ZnO pine tree-like photoanode and CuO/Cu2O nanorod cathode

Lam SM, Sin JC, Warren Tong MW, Zeng H, Li H, Huang L, et al.

Chemosphere, 2023 Dec;344:140402.
PMID: 37838031 DOI: 10.1016/j.chemosphere.2023.140402

Environmental conservation and energy scarcity have become two core challenges with the ever-increasing advancement of industry, particularly chemical energy rich wastewater comprising refractory organics and pathogenic microbes. Here, a multifunctional photocatalytic fuel cell (PFC) was devised using NiFe2O4 nanoparticle-loaded on pine tree-like ZnO/Zn (NiFe2O4/ZnO/Zn) photoanode and CuO/Cu2O nanorods-loaded on Cu (CuO/Cu2O/Cu) cathode for extracting electricity upon wastewater treatment. When fed with Rhodamine B (RhB) dyestuff, the NiFe2O4/ZnO/Zn-PFC provided the maximum power density (Pmax) of 0.539 mW cm-2 upon visible light irradiation with an average RhB degradation of 85.2%, which were 2.8 and 2.7 times higher than ZnO/Zn, respectively. The remarkable enhanced NiFe2O4/ZnO/Zn-PFC performance was owing to the synergistic effect of pine tree-like structure and Z-scheme heterostructure. The pine tree-like with high surface area was not only for effective harnessing photon energies but also provided more directional routes for rapid segregation and transport of carriers and higher interface contacting areas with electrolyte. Through a series of systematic characterizations, the Z-scheme heterostructure mechanism of the system and organics degradation pathway were also speculated. Additionally, the performance of the NiFe2O4/ZnO/Zn-PFC in industry printing wastewater showed Pmax of 0.600 mW cm-2, which was considerably impressive as real wastewater was challenging to accomplish. The phytotoxicity outcome also manifested that the comprehensive toxicity of RhB was eradicated after PFC treatment. Lastly, the excellent recyclability and the pronounced bactericidal effect towards Escherichia coli and Staphylococcus aureus were other attributions which enabled the NiFe2O4/ZnO/Zn-PFC for possible practical application.

MeSH terms: Electrodes; Light; Nanotubes*; Waste Water
Pulmonary alveolar microlithiasis-a rare lung disease-diagnosed by transbronchial cryobiopsy

Kho SS, Chai CS, Ho RL, Ismail AM, Tie ST

Lancet, 2023 Oct 14;402(10410):1356.
PMID: 37838440 DOI: 10.1016/S0140-6736(23)01906-2
MeSH terms: Biopsy; Bronchoscopy; Humans; Lung/pathology
Fulltext Enhancing operational stability of OLEDs based on subatomic modified thermally activated delayed fluorescence compounds

Jung S, Cheung WL, Li SJ, Wang M, Li W, Wang C, et al.

Nat Commun, 2023 Oct 14;14(1):6481.
PMID: 37838720 DOI: 10.1038/s41467-023-42019-6

The realization of operationally stable blue organic light-emitting diodes is a challenging issue across the field. While device optimization has been a focus to effectively prolong device lifetime, strategies based on molecular engineering of chemical structures, particularly at the subatomic level, remains little. Herein, we explore the effect of targeted deuteration on donor and/or acceptor units of thermally activated delayed fluorescence emitters and investigate the structure-property relationship between intrinsic molecular stability, based on isotopic effect, and device operational stability. We show that the deuteration of the acceptor unit is critical to enhance the photostability of thermally activated delayed fluorescence compounds and hence device lifetime in addition to that of the donor units, which is commonly neglected due to the limited availability and synthetic complexity of deuterated acceptors. Based on these isotopic analogues, we observe a gradual increase in the device operational stability and achieve the long-lifetime time to 90% of the initial luminance of 23.4 h at the luminance of 1000 cd m-2 for thermally activated delayed fluorescence-sensitized organic light-emitting diodes. We anticipate our strategic deuteration approach provides insights and demonstrates the importance on structural modification materials at a subatomic level towards prolonging the device operational stability.
Use of a portable negative pressure wound therapy device on a hard-to-heal wound with exposed bone: a case study

Wen CWY, Nasir FABM, Charl MK, Jane CA, Abdullah NSKH, Ping LB, et al.

J Wound Care, 2023 Oct 01;32(Sup10a):S16-S20.
PMID: 37830842 DOI: 10.12968/jowc.2023.32.Sup10a.S16

This case study examines the effectiveness of using negative pressure wound therapy (NPWT) in the management of a hard-to-heal (chronic) wound with exposed ankle bone to reduce associated wound exudate and promote production of granulation tissue. A 60-year-old male patient who was able to attend wound follow-up diligently twice weekly for eight weeks, and weekly thereafter, was selected from a private hospital to take part. During each dressing change, the wound was cleansed with superoxidised cleansing solution, and minimal sharp debridement was performed. In the authors' opinion, the NPWT device used in this study is light and convenient for use in the community or home care setting. The NPWT wound dressing was connected to the NPWT machine via a connecting tube and the device then switched on using the default setting of a negative pressure of 125mmHg. Following the application of the NPWT device, the exposed ankle bone was successfully covered with healthy granulation tissue and healed within 20 weeks with minimal exudate formation in the wound. In the authors' opinion, NPWT is able to promote progress to wound healing; to minimise unnecessary dressing changes and, based on feedback from the patient, is comfortable to wear and when in use.

MeSH terms: Bandages; Exudates and Transudates; Humans; Male; Middle Aged; Surgical Wound Infection; Wound Healing; Negative-Pressure Wound Therapy*
Use of bioelectric dressings for patients with hard-to-heal wounds: a case report

Nair HK, Yan TD

J Wound Care, 2023 Oct 01;32(Sup10a):S8-S14.
PMID: 37830843 DOI: 10.12968/jowc.2023.32.Sup10a.S8

There are many types of dressings available for the management of hard-to-heal (chronic) wounds. This case report illustrates the efficacy of bioelectric dressings in healing hard-to-heal wounds in five patients. Of the patients, four had diabetic foot ulcers (DFUs) and one had a surgical site infection. Wounds were examined using the TIMES concept and debridement was carried out if needed. Amorphous hydrogel was used as conduction fluid before the application of the bioelectric wound dressings. The wound was covered with foam dressing and crepe bandage. In this case report, among all five wounds, one wound healed completely while the other four reduced in size, with the presence of more granulation and re-epithelialisation. In this case report, bioelectric wound dressings were effective in managing infection and promoting wound healing.

MeSH terms: Humans; Wound Healing; Hydrogels; Bandages, Hydrocolloid*; Re-Epithelialization
Real-world evidence is the way to go in resource-limited countries

Nair HK

J Wound Care, 2023 Oct 01;32(Sup10a):S3.
PMID: 37830844 DOI: 10.12968/jowc.2023.32.Sup10a.S3
Run-off modelling of pervious and impervious areas using couple SWAT and a novel machine learning model in cross-rivers state Nigeria

Salele B, Dodo YA, Sani DA, Abuhussain MA, Sayfutdinovna Abdullaeva B, Brysiewicz A

Water Sci Technol, 2023 Oct;88(7):1893-1909.
PMID: 37831003 DOI: 10.2166/wst.2023.304

Using the soil and water assessment tool (SWAT), runoff in pervious and impervious urban areas was simulated in this study. In the meantime, as a novel application of machine learning, the emotional artificial neural network (EANN) model was employed to enhance the SWAT obtained for this study. As a result of the EANN model's capabilities in rainfall-runoff phenomena, the SWAT-EANN couple model has been used to assess urban flooding. The pervious, impervious, and water body areas of the study area were classified and mapped to estimate the cover change over three epochs. Land use map, precipitation data, temperature (minimum and maximum) data, wind speed, relative humidity, soil map, solar radiation, and digital elevation model were used as inputs for modelling rainfall-runoff of the study area in the ArcGIS environment. The accuracy assessment of this study was excellent (root-mean-square error 1 mm of precipitation). It also revealed that (a) a land use map illustrating changes in impervious, pervious surface, and water body for 1998, 2008, and 2018; (b) runoff modelling using a historical pattern of rainfall-runoff changes (1998-2018); and (c) descriptive statistical analysis of the runoff results of the research. This research will aid in urban planning, administration, and development. Specifically, it will prevent flooding and environmental problems.

MeSH terms: Nigeria; Soil*; Water*; Water Movements; Floods
Cost-effective complete genome sequencing using the MinION platform for identification of recombinant enteroviruses

Chien YS, Chen FJ, Wu HC, Lin CH, Chang WC, Perera D, et al.

Microbiol Spectr, 2023 Oct 13.
PMID: 37831475 DOI: 10.1128/spectrum.02507-23

Enteroviruses (EVs) are a group of viruses that cause various human illnesses. While the CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) method can generate VP1 gene fragments for enterovirus genotyping, it is unable to detect recombinant strains. Recent advances in viral genome sequencing using next-generation sequencing technologies have enabled comprehensive analyses. However, the high cost poses a challenge for widespread adoption. To address this issue, this study proposes a cost-effective approach for generating complete enterovirus genome sequences using the Oxford Nanopore MinION sequencer. This protocol not only facilitates the generation of accurate genome sequences for various enterovirus strains but also allows for the differentiation of co-infections from viral isolates. In addition, the method can generate polyprotein sequences as well as peptide sequences of the upstream ORF (uORF) whose expression can impact virus infection. Through the analysis of complete enterovirus genomes, this study successfully identified seven enterovirus A71 isolates obtained during the 2018 enterovirus outbreak in Malaysia and Taiwan as recombinants between enterovirus A71 and coxsackievirus A2. Furthermore, our study has made a significant discovery by establishing a strong correlation between uORF trees and the epidemics of EVA71. This finding highlights the potential of uORF sequences as valuable indicators for monitoring and understanding the spread of EVA71 infections. We also identified notable amino acid changes in the transmembrane domain of the uORF protein within a newly identified lineage. These findings provide crucial insights into the molecular characteristics and evolutionary dynamics of EVA71, offering valuable information for future research and intervention strategies. IMPORTANCE By employing a cost-effective approach for complete genome sequencing, the study has enabled the identification of novel enterovirus strains and shed light on the genetic exchange events during outbreaks. The success rate of genome sequencing and the scalability of the protocol demonstrate its practical utility for routine enterovirus surveillance. Moreover, the study's findings of recombinant strains of EVA71 and CVA2 contributing to epidemics in Malaysia and Taiwan emphasize the need for accurate detection and characterization of enteroviruses. The investigation of the whole genome and upstream ORF sequences has provided insights into the evolution and spread of enterovirus subgenogroups. These findings have important implications for the prevention, control, and surveillance of enteroviruses, ultimately contributing to the understanding and management of enterovirus-related illnesses.

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