Browse publications by year: 2024

  1. Wong YP, Tee KK, Chee HY
    Int Microbiol, 2024 Dec 02.
    PMID: 39623085 DOI: 10.1007/s10123-024-00621-8
    Rhinoviruses (RVs), particularly RV-C, frequently cause acute respiratory infections and asthma exacerbations. However, there is a lack of routine detection methods. Thus, this study aims to develop a rapid molecular and differential diagnostic detection method for RV-C using the reverse transcription (RT) loop-mediated isothermal amplification (LAMP) approach. The RT-LAMP assay targeting the 5'UTR region of RV-C genome was optimized by varying the reaction temperature, magnesium sulphate, betaine concentrations, and reaction time. Compared with conventional RT-PCR with a sensitivity of 106 copies of RNA, RT-LAMP demonstrated a significant increase in efficiency and sensitivity with a quantifiable viral load of at least 101 copies of RNA by gel electrophoresis and colour change, and 104 copies of RNA for end-point detection with a turbidimeter for 40 min. The assay is also specific without amplifying RV-A16 and RV-B72 genomic RNA. In the proof-of-concept assay using 30 clinical respiratory samples with known etiological agents, it detected all RV-C isolates, of which its accuracy was confirmed by sequencing. The newly developed RT-LAMP assay demonstrated good analytical sensitivity and specificity toward RV-C. The assay provides an alternative for improved RV-C diagnosis.
  2. Singh JG, Singh AK, Idris NHM, Kitchawengkul N, Jarujamrus P, Lee HL, et al.
    Discov Nano, 2024 Dec 02;19(1):196.
    PMID: 39623210 DOI: 10.1186/s11671-024-04117-2
    In the last decade, carbon quantum dots (CQDs), a novel class of carbon-based nanomaterials, have received increasing attention due to their distinct properties. Carbon Quantum Dots/Titanium Dioxide (CQDs/TiO2) Nanocomposites were reported as potent compounds against SARS-COV-2. In this manuscript, citric acid is the carbon precursor used to synthesize carbon quantum dots (CQDs). Using a green approach, the synthesized CQD fabricates the Carbon Quantum Dots/Titanium Dioxide (CQDs/TiO2) Nanocomposites. Synthesized composites were characterized by using a UV-visible spectrophotometer, Fourier-transformed infrared (FTIR) spectrometry, X-ray diffractometry (XRD), and Scanning electron microscopy with energy dispersive X-ray analysis (SEM-EDX). Methylene blue was used to check the Photocatalytic activity of synthesized (CQDs/TiO2) nanocomposites of different concentrations. Computational modeling of agglomerates of CQD and TiO2 nanoparticles with the formula TiO2…….. Ti253O506 demonstrated two stages of the nanocomposite formation, including the formation of agglomerates with the neutral and salt-like structures with the total gain in the Gibbs free energy - 38.397 kcal/mole. In silico, Molecular docking studies of citric acid were evaluated against SARS-COV-2 protein to understand their mechanism and key amino acid interactions along with standard drug remdesivir. The photocatalytic activity of CQDs/TiO2 showed extremely promising results. Based on this study, the proposed mechanism of action of these compounds is reported. A detailed investigation of CQDs/TiO2 against SARS-CoV-2 is needed, which is another part of the research in our next manuscript.
  3. Nisa ZU, Zeshan B, Ambreen A, Mustafa T
    BMC Infect Dis, 2024 Dec 02;24(1):1375.
    PMID: 39623309 DOI: 10.1186/s12879-024-10228-z
    BACKGROUND: We aimed to assess the plasma levels of ferritin, C-reactive protein (CRP), and adenosine deaminase (ADA) at baseline and their utility as biomarkers to monitor response to treatment in extrapulmonary tuberculosis (EPTB) patients.

    METHODS: Prospective measurements of ferritin, CRP, and ADA were done in unstimulated plasma samples of 92 EPTB (49 TB lymphadenitis and 43 TB pleuritis) patients registered for anti-TB treatment. Blood samples were taken at the start, 2, and 6 months of treatment, plasma levels of ferritin and CRP were measured by the enzyme-linked immunosorbent assay and ADA levels by kinetic chemistry method at each time point. Data was analyzed using SPSS version 22. Non-parametric tests were used for paired analysis and two groups' comparison. Spearman's rank test was used for correlation analysis. A Chi-square test was used for categorical variables. A p-value 

    MeSH terms: Adolescent; Adult; Aged; Female; Humans; Male; Middle Aged; Prospective Studies; Tuberculosis, Pleural/blood; Tuberculosis, Pleural/drug therapy; Treatment Outcome; Young Adult
  4. Rana S, Wahab NA, Shima Shahidan WN, Atif S, Fahim A
    J Ayub Med Coll Abbottabad, 2024;36(3):636-641.
    PMID: 39623849 DOI: 10.55519/JAMC-03-12344
    Inflammatory biomarkers are molecules that can offer vital information on the intricate chain of happenings and molecular processes underpinning the pathophysiology of any inflammatory disease. They can be measured in various biological samples such as blood, urine, or saliva, and are used as indicators of the presence and severity of inflammation. Measuring salivary inflammatory biomarkers is a non-invasive and relatively easy way to monitor inflammation, and it has been shown to be a useful tool in the diagnosis and management of various oral and systemic inflammatory diseases. Irisin is a novel anti-inflammatory protein and its implication and diagnostic role in inflammation have been widely studied; however, not much have been studied in oral inflammation per se. Irisin is predominantly downregulated in several inflammatory conditions, including obesity, type 2 diabetes, periodontitis, and cardiovascular diseases. This suggests that irisin may be involved in the inflammatory process, but more research is needed, especially of salivary irisin to understand its precise role. Overall, the role of irisin as an inflammatory biomarker is still an area of active research, and more studies are needed to determine its diagnostic and therapeutic potential. This review highlights the diagnostic and therapeutic potential of irisin in various systemic and oral inflammatory conditions.
    MeSH terms: Diabetes Mellitus, Type 2/blood; Diabetes Mellitus, Type 2/diagnosis; Humans; Obesity; Periodontitis/diagnosis; Saliva/chemistry
  5. Koh EJ, Chin ML, Ab Allah NA
    J Ayub Med Coll Abbottabad, 2024;36(3):657-660.
    PMID: 39623852 DOI: 10.55519/JAMC-03-12883
    Immune mediated necrotising myopathy (IMNM) is a rare autoimmune disease of the muscles belonging to the subset of the idiopathic inflammatory myopathies (IIM). This disease entity has classically been associated with myositis specific antibodies. The hallmark feature in clinching the diagnosis of IMNM would be a muscle biopsy showing muscle necrosis and regeneration in the absence of significant inflammatory infiltrates, interpreted in an appropriate clinical context. The term 'neuromyositis' was previously coined in the year 1893 to describe a concomitant polyneuropathy in patients with polymyositis or dermatomyositis. However, a combined polyneuropathy with IMNM has never been reported in previous literature. We describe a case of a 35-year-old gentleman who presented with a 5-day history of symmetrical bilateral lower limb pain and weakness. Despite a negative autoimmune work-up, his muscle biopsy was suggestive of IMNM. A nerve conduction study done had also revealed a superimposed non-length dependant axonal polyneuropathy. The patient had responded well to steroids and is now under remission. This case serves to highlight a rare entity of seronegative IMNM superimposed with an axonal polyneuropathy.
    MeSH terms: Adult; Humans; Male; Myositis/complications; Myositis/diagnosis; Necrosis
  6. Kumar P, Jayan J, Balaraman AK, Pandey S, Brar M, Mehta R, et al.
    New Microbes New Infect, 2024 Dec;62:101530.
    PMID: 39624228 DOI: 10.1016/j.nmni.2024.101530
  7. Ndabashinze R, Dey RC, Brar M, Sah S, Mehta R, Bushi G, et al.
    New Microbes New Infect, 2024 Dec;62:101527.
    PMID: 39624229 DOI: 10.1016/j.nmni.2024.101527
  8. Rezaei M, Salari N, Aznab M, Jasmi SV, Abdi A, Shohaimi S
    Heliyon, 2024 Nov 30;10(22):e40283.
    PMID: 39624272 DOI: 10.1016/j.heliyon.2024.e40283
    BACKGROUND: Patients with gastrointestinal cancers commonly experience acute and chronic pain. This study aimed to determine the effect of deep diaphragmatic breathing on acute and chronic pain in patients with metastatic gastrointestinal cancers.

    METHODS: This clinical trial was conducted in Kermanshah-Iran in 2022. The sample consisted of 44 patients hospitalized in the oncology ward, who were selected by convenient sampling and randomly allocated into case and control groups. The case group performed diaphragmatic deep breathing intervention for 10 days, twice a day, for 10 min. The subjects completed pain assessment tools before and after the intervention. Data were analyzed using SPSS Version 24.

    RESULTS: The study included participants with a Mean and Standard Deviation(SD) age of 53.95 ± 10.51 years. The case and control groups were similar in terms of demographic variables. The mean and sd acute pain score before the intervention was 3.50 ± 1.84 in the experimental group and 2.18 ± 1.65 in the control group (p = 0.01). However, after the intervention, the score decreased to 1.72 ± 1.07 in the experimental group and increased to 3.72 ± 1.95 in the control group (p = 0.001). The two groups did not differ significantly in terms of chronic pain before the intervention (p = 0.07). However, after the intervention, the score decreased in the experimental group and increased in the control group, with a significant difference (p = 0.01).

    CONCLUSION: The results of this study suggest that deep diaphragmatic breathing reduces pain in patients with gastrointestinal cancer. Including this method as a routine care program for cancer patients is recommended.

  9. Sharma T, Arora G, Ng CY, Jun HK
    Heliyon, 2024 Nov 30;10(22):e40341.
    PMID: 39624276 DOI: 10.1016/j.heliyon.2024.e40341
    Considering global climate change concerns, issues related to the energy crisis and technologies reliant on non-fossil renewable energy sources are in high demand. Solar energy emerges as one of the alternatives among all renewable energy resources due to its economic viability and environmental sustainability. There are various types of solar photovoltaic (PV) technologies available for commercial applications, such as organic solar cells, silicon-based solar cells, dye-sensitized solar cells, and perovskite solar cells. Notably, one of the drawbacks of PV devices is their inability to generate power at night or during cloudy days (i.e., low-light conditions). One solution to this problem is to provide backup to the PV devices, such as batteries or energy storage packs. Another less explored alternative backup is the application of long persistent luminescence (LPL) materials as a secondary light source or down shifter. LPL materials can provide an afterglow that can last for hours which can be harvested by PV devices for power generation under low-light conditions. This short overview article discusses the fundamental mechanisms of LPL materials and the feasibility and challenges of integrating LPL materials into PV, which is hoped can provide useful insights for future research directions.
  10. Juhari ML, Arifin K, Aiyub K, Ismail ZS
    Heliyon, 2024 Nov 30;10(22):e40396.
    PMID: 39624297 DOI: 10.1016/j.heliyon.2024.e40396
    Facility management is essential in modern office settings, as it helps to ensure the safety and health of employees. Issues related to facility management weaknesses, such as facility damage, structural issues, ventilation problems, and more, are subjects of ongoing concern. Therefore, safety and health practices are important aspects of facility management to ensure that the office is always safe and healthy for employees. Objectives: This research aims to address these concerns by developing a comprehensive model for safety and health practices in building, focusing on the physical environment, facility management, and worker perception. Methods: Data was gathered through random survey questionnaires distributed to government offices within the federal territory of Putrajaya, Malaysia. The survey covered 156 measurement items across 18 parameters, encompassing three dimensions: facility management, the physical environment, and office workers' perceptions. A total of 562 valid responses were collected. The data was analyzed using Structural Equation Modeling (SEM), which integrates path analysis and confirmatory factor analysis (CFA), through the IBM SPSS-AMOS software. Results: The results of this research indicate that the analysis of the model's goodness of fit proves that all variables within the model, whether independent or dependent are fitted and can be adapted. Consequently, the safety and health practices in building model was successfully developed. Conclusion: This model offers a valuable tool for facility management organizations, enabling them to systematically assess and maintain safe and healthy workplace conditions. It signifies a significant step forward in ensuring employee well-being in office buildings.
  11. George PM, Rennison-Jones C, Benvenuti G, Sifostratoudaki A, Ottink FA, Bou-Zeid W, et al.
    ERJ Open Res, 2024 Nov;10(6).
    PMID: 39624378 DOI: 10.1183/23120541.00570-2024
    BACKGROUND: In patients with idiopathic pulmonary fibrosis (IPF) there is a need to identify biomarkers that 1) are associated with increased risk of adverse outcome and 2) can be used to monitor treatment response or identify disease progression over time.

    METHODS: Two consecutive cohorts of patients with IPF were accessed from the Open Source Imaging Consortium database. Automated computed tomography (CT) biomarkers of disease severity incorporating fibrotic and pulmonary vascular features (the reticulovascular score and weighted reticulovascular score (WRVS)) were studied. Relationships between imaging biomarkers, lung function and survival were analysed.

    RESULTS: In separate test and validation cohorts, 168 and 176 patients with IPF respectively (median survival 2.6 years) were studied. A threshold of WRVS ≥15% at baseline CT was most strongly associated with transplant-free survival (HR 3.00, 95% CI 1.47-6.10, p=0.002) when adjusted for baseline forced vital capacity (FVC) and age. In patients with 12-month follow-up CT and lung function tests (n=89) an increase in 3% of WRVS (the minimal clinically important difference) was also significantly associated with reduced survival independent of FVC, and outperformed visual evaluation of progressive fibrosis.

    CONCLUSIONS: WRVS is an automated CT biomarker which can identify patients with IPF at increased risk of progression and is able to reliably capture disease progression over time.

  12. Zhu SR, Feng XJ, Zheng WK, Niu XR, Mo ST, Tang WZ
    Clin Interv Aging, 2024;19:1979-1995.
    PMID: 39624803 DOI: 10.2147/CIA.S487329
    BACKGROUND: The significant occurrence of oral frailty (OF) in older patients highlights the necessity of addressing this concern. OF is associated with complications and mortality in elderly patients. The objective of this study is to identify unfavorable outcomes in older patients with OF to increase awareness and mitigate risks.

    METHODS: This review employed specific MeSH terms to perform an extensive literature search on OF and its unfavorable outcomes across PubMed, Web of Science, Embase, Cochrane Library, Scopus, and CINAHL databases. The review included English-language papers published from inception to March 31, 2024, focusing on individuals aged 60 and above, adverse outcomes related to OF, and studies employing cross-sectional or cohort designs.

    RESULTS: The review comprised 28 articles: 20 cross-sectional and 8 prospective cohort studies. Among these articles, 10 were rated as "Good" and 18 as "Fair", reflecting the high quality of the literature. Next, 20 OF assessment tools were summarized, and the most frequently used methods for assessing OF were the methods of Tanaka et al and Oral Frailty Index-8 (OF-8). The most frequently unfavorable outcomes related to OF in the elderly were physical frailty, malnutrition, low dietary variety, social withdrawal, disability, and low gait speed.

    CONCLUSION: High levels of OF significantly increase the risk of adverse outcomes in older adults, including physical frailty, malnutrition, low dietary variety, social withdrawal, disability, and low gait speed. Effective risk stratification and management are essential to reduce these outcomes.

    MeSH terms: Aged; Humans; Geriatric Assessment*; Frail Elderly*; Malnutrition
  13. Salazar de Pablo G, Iniesta R, Bellato A, Caye A, Dobrosavljevic M, Parlatini V, et al.
    Mol Psychiatry, 2024 Dec;29(12):3865-3873.
    PMID: 38783054 DOI: 10.1038/s41380-024-02606-5
    There have been increasing efforts to develop prediction models supporting personalised detection, prediction, or treatment of ADHD. We overviewed the current status of prediction science in ADHD by: (1) systematically reviewing and appraising available prediction models; (2) quantitatively assessing factors impacting the performance of published models. We did a PRISMA/CHARMS/TRIPOD-compliant systematic review (PROSPERO: CRD42023387502), searching, until 20/12/2023, studies reporting internally and/or externally validated diagnostic/prognostic/treatment-response prediction models in ADHD. Using meta-regressions, we explored the impact of factors affecting the area under the curve (AUC) of the models. We assessed the study risk of bias with the Prediction Model Risk of Bias Assessment Tool (PROBAST). From 7764 identified records, 100 prediction models were included (88% diagnostic, 5% prognostic, and 7% treatment-response). Of these, 96% and 7% were internally and externally validated, respectively. None was implemented in clinical practice. Only 8% of the models were deemed at low risk of bias; 67% were considered at high risk of bias. Clinical, neuroimaging, and cognitive predictors were used in 35%, 31%, and 27% of the studies, respectively. The performance of ADHD prediction models was increased in those models including, compared to those models not including, clinical predictors (β = 6.54, p = 0.007). Type of validation, age range, type of model, number of predictors, study quality, and other type of predictors did not alter the AUC. Several prediction models have been developed to support the diagnosis of ADHD. However, efforts to predict outcomes or treatment response have been limited, and none of the available models is ready for implementation into clinical practice. The use of clinical predictors, which may be combined with other type of predictors, seems to improve the performance of the models. A new generation of research should address these gaps by conducting high quality, replicable, and externally validated models, followed by implementation research.
    MeSH terms: Attention Deficit Disorder with Hyperactivity*; Humans; Prognosis
  14. Huneke NTM, Amin J, Baldwin DS, Bellato A, Brandt V, Chamberlain SR, et al.
    Mol Psychiatry, 2024 Dec;29(12):3915-3925.
    PMID: 38914807 DOI: 10.1038/s41380-024-02638-x
    There is a growing literature exploring the placebo response within specific mental disorders, but no overarching quantitative synthesis of this research has analyzed evidence across mental disorders. We carried out an umbrella review of meta-analyses of randomized controlled trials (RCTs) of biological treatments (pharmacotherapy or neurostimulation) for mental disorders. We explored whether placebo effect size differs across distinct disorders, and the correlates of increased placebo effects. Based on a pre-registered protocol, we searched Medline, PsycInfo, EMBASE, and Web of Knowledge up to 23.10.2022 for systematic reviews and/or meta-analyses reporting placebo effect sizes in psychopharmacological or neurostimulation RCTs. Twenty meta-analyses, summarising 1,691 RCTs involving 261,730 patients, were included. Placebo effect size varied, and was large in alcohol use disorder (g = 0.90, 95% CI [0.70, 1.09]), depression (g = 1.10, 95% CI [1.06, 1.15]), restless legs syndrome (g = 1.41, 95% CI [1.25, 1.56]), and generalized anxiety disorder (d = 1.85, 95% CI [1.61, 2.09]). Placebo effect size was small-to-medium in obsessive-compulsive disorder (d = 0.32, 95% CI [0.22, 0.41]), primary insomnia (g = 0.35, 95% CI [0.28, 0.42]), and schizophrenia spectrum disorders (standardized mean change = 0.33, 95% CI [0.22, 0.44]). Correlates of larger placebo response in multiple mental disorders included later publication year (opposite finding for ADHD), younger age, more trial sites, larger sample size, increased baseline severity, and larger active treatment effect size. Most (18 of 20) meta-analyses were judged 'low' quality as per AMSTAR-2. Placebo effect sizes varied substantially across mental disorders. Future research should explore the sources of this variation. We identified important gaps in the literature, with no eligible systematic reviews/meta-analyses of placebo response in stress-related disorders, eating disorders, behavioural addictions, or bipolar mania.
    MeSH terms: Humans; Placebo Effect*; Randomized Controlled Trials as Topic
  15. Phang HC, Ng ZQ, Mohamad N, Chew YL, Balaraman A, Kee PE, et al.
    Drug Dev Ind Pharm, 2024 Sep;50(9):810-826.
    PMID: 39320267 DOI: 10.1080/03639045.2024.2409168
    BACKGROUND: Quetiapine fumarate (QTP) is commonly prescribed for schizophrenic patient, typically available in tablet or oral suspension form, presenting challenges such as administration difficulties, fear of choking and distaste for its bitter taste. Fast melt films (FMF) offer an alternative dosage form with a simple development process, ease of administration and rapid drug absorption and action onset.

    OBJECTIVE: This study aims to prepare FMF with different formulations using solvent casting methods and to compare the effects of different drying methods, including oven drying and freeze drying, on the properties of the films.

    METHODS: Various formulations were created by manipulating polymer types (starch, hydroxypropyl methylcellulose (HPMC) and guar gum) at different concentrations, along with fixed concentrations of QTP and other excipients. Characterization tests including surface morphology, weight, thickness, pH, tensile strength, elongation length, Young's modulus, folding endurance and disintegration time were conducted. The optimal FMF formulation was identified and further evaluated for moisture and drug content, dissolution behavior, accelerated stability, X-ray diffraction (XRD), and palatability.

    RESULTS: FMF containing 10 mg guar gum/film developed using oven drying emerged as the optimum choice, exhibiting desirable film appearance, ultra-thin thickness (0.453 ± 0.002 mm), appropriate pH for oral intake (pH 5.0), optimal moisture content of 11.810%, rapid disintegration (52.67 ± 1.53 s), high flexibility (folding endurance > 300 times) and lower Young's modulus (1.308 ± 0.214).

    CONCLUSION: Oven drying method has been proven to be favorable for developing FMF containing QTP, meeting all testing criteria and providing an alternative option for QTP prescription.

    MeSH terms: Chemistry, Pharmaceutical/methods; Desiccation/methods; Drug Compounding/methods; Mannans/chemistry; Polymers/chemistry; Solubility; Tensile Strength; Antipsychotic Agents/administration & dosage; Antipsychotic Agents/chemistry; X-Ray Diffraction/methods; Hypromellose Derivatives/chemistry
  16. Ho C, Mohd Yusof BN, Abdul Majid H, Daud ZAM
    Clin Nutr ESPEN, 2024 Dec;64:168-176.
    PMID: 39362334 DOI: 10.1016/j.clnesp.2024.09.022
    BACKGROUND: Gynecologic cancer (GC) patients often experience systemic inflammation, malnutrition, and compromised postoperative outcomes. This systematic review aims to comprehensively synthesize existing data regarding the impact of perioperative immunonutrition (IMN) intervention on GC patients.

    METHODS: The databases of CENTRAL, EMBASE, Web of Science, and the Cochrane Library were used to conduct a literature search, supplemented by internet search engines and manual searches. Publications released between January 2009 and October 2023 was identified, reviewed, and data extracted.

    RESULTS: The review encompasses six studies involving 712 patients, comprising two randomized controlled trials (RCTs), two prospective studies, and two retrospective cohort studies. Three studies prescribed IMN perioperatively; two pre-operatively and one post-operatively. Four out of six studies reported less post-operative infection and complications. Two studies reported shorter hospitalization using the IMN formula. One study reported a longer hospitalization with IMN supplementation. Overall survival showed no significant difference in the two studies. Four studies reported positive modulation of inflammatory markers and lymphocytes as outcomes, with IMN formulas.

    DISCUSSION AND CONCLUSION: Perioperative IMN emerge as a promising intervention, demonstrating notable benefits included shortened hospitalization as well and positive modulation of inflammatory markers.

    MeSH terms: Female; Humans; Inflammation; Randomized Controlled Trials as Topic; Malnutrition
  17. Tan JP, Clyde CW, Ng CC, Yeap SK, Yong CY
    J Environ Radioact, 2024 Dec;280:107530.
    PMID: 39378736 DOI: 10.1016/j.jenvrad.2024.107530
    The global production of radioactive wastes is expected to increase in the coming years as more countries have resorted to adopting nuclear power to decrease their reliance on fossil-fuel-generated energy. Discoveries of remediation methods that can remove radionuclides from radioactive wastes, including those discharged to the environment, are therefore vital to reduce risks-upon-exposure radionuclides posed to humans and wildlife. Among various remediation approaches available, microbe-mediated radionuclide remediation have limited reviews regarding their advances. This review provides an overview of the sources and existing classification of radioactive wastes, followed by a brief introduction to existing radionuclide remediation (physical, chemical, and electrochemical) approaches. Microbe-mediated radionuclide remediation (bacterial, myco-, and phycoremediation) is then extensively discussed. Bacterial remediation involves biological processes like bioreduction, biosorption, and bioprecipitation. Bioreduction involves the reduction of water-soluble, mobile radionuclides to water-insoluble, immobile lower oxidation states by ferric iron-reducing, sulfate-reducing, and certain extremophilic bacteria, and in situ remediation has become possible by adding electron donors to contaminated waters to enrich indigenous iron- and sulfate-reducing bacteria populations. In biosorption, radionuclides are associated with functional groups on the microbial cell surface, followed by getting reduced to immobilized forms or precipitated intracellularly or extracellularly. Myco- and phycoremediation often involve processes like biosorption and bioaccumulation, where the former is influenced by pH and cell concentration. A Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis on microbial remediation is also performed. It is suggested that two research directions: genetic engineering of radiation-resistant microorganisms and co-application of microbe-mediated remediation with other remediation methods could potentially result in the discovery of in situ or ex situ microbe-involving radioactive waste remediation applications with high practicability. Finally, a comparison between the strengths and weaknesses of each approach is provided.
    MeSH terms: Bacteria/metabolism; Biodegradation, Environmental*; Radioactive Waste*
  18. de Almeida Santos G, Englund ANB, Dalleywater EL, Røhr ÅK
    FEBS Open Bio, 2024 Dec;14(12):2038-2058.
    PMID: 39382070 DOI: 10.1002/2211-5463.13906
    Tyrosinases (TYRs) are type-3 copper proteins that are widely distributed in nature. They can hydroxylate and oxidize phenolic molecules and are mostly known for producing melanins that confer protection against photo induced damage. TYRs are also thought to play an important role in the 'latch mechanism', where high concentrations of phenolic compounds inhibit oxidative decomposition of organic biomass and subsequent CO2 release, especially relevant in wetland environments. In the present study, we describe two TYRs, HcTyr1 and HcTyr2, from halophilic bacterium Hahella sp. CCB MM4 previously isolated at Matang mangrove forest in Perak, Malaysia. The structure of HcTyr1 was determined by X-ray crystallography at a resolution of 1.9 Å and represents an uncharacterized group of prokaryotic TYRs as demonstrated by a sequence similarity network analysis. The genes encoding the enzymes were cloned, expressed, purified and thoroughly characterized by biochemical methods. HcTyr1 was able to self-cleave its lid-domain (LID) in a protease independent manner, whereas the LID of HcTyr2 was essential for activity and stability. Both enzymes showed variable activity in the presence of different metals, surfactants and NaCl, and were able to oxidize lignin constituents. The high salinity tolerance of HcTyr1 indicates that the enzyme can be an efficient catalyst in the habitat of the host.
    MeSH terms: Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/chemistry; Oxidation-Reduction*; Monophenol Monooxygenase/metabolism; Crystallography, X-Ray; Wetlands*
  19. Loke YH, Phang HC, Gobal G, Vijayaraj Kumar P, Kee PE, Widodo RT, et al.
    Drug Dev Ind Pharm, 2024 Oct;50(10):845-855.
    PMID: 39418138 DOI: 10.1080/03639045.2024.2417999
    INTRODUCTION: Fast melt tablets (FMTs) provide a convenient dosage form that rapidly dissolves on the tongue without the need for water. Cocoa butter serves as a suitable matrix system for FMTs formulation, facilitating rapid disintegration at body temperature.

    OBJECTIVES: This study aimed to formulate FMTs using cocoa butter as a base and investigate the effect of various disintegrants and superdisintegrants on their characteristics.

    METHODS: Cocoa butter-based FMTs were prepared via the fusion molding technique. Different disintegrants and superdisintegrants were added at varying concentrations and subjected to characterization. The optimal formulation was selected and incorporated with 10 mg memantine hydrochloride.

    RESULTS: The optimal FMT formulation consisted of 340 mg cocoa butter, 75 mg starch, and 75 mg crospovidone, exhibiting a hardness of 17.12 ± 0.31 N and a disintegration time of 32.67 ± 0.17 s. Furthermore, FMTs demonstrated a faster release profile compared to the commercially available product, Ebixa. SEM micrographs revealed homogenous blending of individual ingredients within the cocoa butter matrix and FT-IR analysis confirmed the chemical stability of memantine hydrochloride in the formulation. The dissolution profile of F17 suggested that the drug in FMTs released faster compared to Ebixia. Memantine hydrochloride achieved 98.07% of drug release in FMTs at 10 min. Moreover, the prepared FMTs exhibited stability for at least 6 months.

    CONCLUSION: The successful development of cocoa butter-based FMTs containing memantine hydrochloride highlights the potential of cocoa butter as viable alternative matrix-forming material for FMTs production. This innovative formulation offers patients a convenient alternative for medication administration.

    MeSH terms: Chemistry, Pharmaceutical/methods; Dietary Fats; Drug Stability; Hardness; Povidone/chemistry; Solubility; Starch/chemistry; Tablets*; Spectroscopy, Fourier Transform Infrared/methods; Drug Liberation
External Links