Browse publications by year: 2024

  1. Salazar de Pablo G, Iniesta R, Bellato A, Caye A, Dobrosavljevic M, Parlatini V, et al.
    Mol Psychiatry, 2024 Dec;29(12):3865-3873.
    PMID: 38783054 DOI: 10.1038/s41380-024-02606-5
    There have been increasing efforts to develop prediction models supporting personalised detection, prediction, or treatment of ADHD. We overviewed the current status of prediction science in ADHD by: (1) systematically reviewing and appraising available prediction models; (2) quantitatively assessing factors impacting the performance of published models. We did a PRISMA/CHARMS/TRIPOD-compliant systematic review (PROSPERO: CRD42023387502), searching, until 20/12/2023, studies reporting internally and/or externally validated diagnostic/prognostic/treatment-response prediction models in ADHD. Using meta-regressions, we explored the impact of factors affecting the area under the curve (AUC) of the models. We assessed the study risk of bias with the Prediction Model Risk of Bias Assessment Tool (PROBAST). From 7764 identified records, 100 prediction models were included (88% diagnostic, 5% prognostic, and 7% treatment-response). Of these, 96% and 7% were internally and externally validated, respectively. None was implemented in clinical practice. Only 8% of the models were deemed at low risk of bias; 67% were considered at high risk of bias. Clinical, neuroimaging, and cognitive predictors were used in 35%, 31%, and 27% of the studies, respectively. The performance of ADHD prediction models was increased in those models including, compared to those models not including, clinical predictors (β = 6.54, p = 0.007). Type of validation, age range, type of model, number of predictors, study quality, and other type of predictors did not alter the AUC. Several prediction models have been developed to support the diagnosis of ADHD. However, efforts to predict outcomes or treatment response have been limited, and none of the available models is ready for implementation into clinical practice. The use of clinical predictors, which may be combined with other type of predictors, seems to improve the performance of the models. A new generation of research should address these gaps by conducting high quality, replicable, and externally validated models, followed by implementation research.
    MeSH terms: Attention Deficit Disorder with Hyperactivity*; Humans; Prognosis
  2. Huneke NTM, Amin J, Baldwin DS, Bellato A, Brandt V, Chamberlain SR, et al.
    Mol Psychiatry, 2024 Dec;29(12):3915-3925.
    PMID: 38914807 DOI: 10.1038/s41380-024-02638-x
    There is a growing literature exploring the placebo response within specific mental disorders, but no overarching quantitative synthesis of this research has analyzed evidence across mental disorders. We carried out an umbrella review of meta-analyses of randomized controlled trials (RCTs) of biological treatments (pharmacotherapy or neurostimulation) for mental disorders. We explored whether placebo effect size differs across distinct disorders, and the correlates of increased placebo effects. Based on a pre-registered protocol, we searched Medline, PsycInfo, EMBASE, and Web of Knowledge up to 23.10.2022 for systematic reviews and/or meta-analyses reporting placebo effect sizes in psychopharmacological or neurostimulation RCTs. Twenty meta-analyses, summarising 1,691 RCTs involving 261,730 patients, were included. Placebo effect size varied, and was large in alcohol use disorder (g = 0.90, 95% CI [0.70, 1.09]), depression (g = 1.10, 95% CI [1.06, 1.15]), restless legs syndrome (g = 1.41, 95% CI [1.25, 1.56]), and generalized anxiety disorder (d = 1.85, 95% CI [1.61, 2.09]). Placebo effect size was small-to-medium in obsessive-compulsive disorder (d = 0.32, 95% CI [0.22, 0.41]), primary insomnia (g = 0.35, 95% CI [0.28, 0.42]), and schizophrenia spectrum disorders (standardized mean change = 0.33, 95% CI [0.22, 0.44]). Correlates of larger placebo response in multiple mental disorders included later publication year (opposite finding for ADHD), younger age, more trial sites, larger sample size, increased baseline severity, and larger active treatment effect size. Most (18 of 20) meta-analyses were judged 'low' quality as per AMSTAR-2. Placebo effect sizes varied substantially across mental disorders. Future research should explore the sources of this variation. We identified important gaps in the literature, with no eligible systematic reviews/meta-analyses of placebo response in stress-related disorders, eating disorders, behavioural addictions, or bipolar mania.
    MeSH terms: Humans; Placebo Effect*; Randomized Controlled Trials as Topic
  3. Phang HC, Ng ZQ, Mohamad N, Chew YL, Balaraman A, Kee PE, et al.
    Drug Dev Ind Pharm, 2024 Sep;50(9):810-826.
    PMID: 39320267 DOI: 10.1080/03639045.2024.2409168
    BACKGROUND: Quetiapine fumarate (QTP) is commonly prescribed for schizophrenic patient, typically available in tablet or oral suspension form, presenting challenges such as administration difficulties, fear of choking and distaste for its bitter taste. Fast melt films (FMF) offer an alternative dosage form with a simple development process, ease of administration and rapid drug absorption and action onset.

    OBJECTIVE: This study aims to prepare FMF with different formulations using solvent casting methods and to compare the effects of different drying methods, including oven drying and freeze drying, on the properties of the films.

    METHODS: Various formulations were created by manipulating polymer types (starch, hydroxypropyl methylcellulose (HPMC) and guar gum) at different concentrations, along with fixed concentrations of QTP and other excipients. Characterization tests including surface morphology, weight, thickness, pH, tensile strength, elongation length, Young's modulus, folding endurance and disintegration time were conducted. The optimal FMF formulation was identified and further evaluated for moisture and drug content, dissolution behavior, accelerated stability, X-ray diffraction (XRD), and palatability.

    RESULTS: FMF containing 10 mg guar gum/film developed using oven drying emerged as the optimum choice, exhibiting desirable film appearance, ultra-thin thickness (0.453 ± 0.002 mm), appropriate pH for oral intake (pH 5.0), optimal moisture content of 11.810%, rapid disintegration (52.67 ± 1.53 s), high flexibility (folding endurance > 300 times) and lower Young's modulus (1.308 ± 0.214).

    CONCLUSION: Oven drying method has been proven to be favorable for developing FMF containing QTP, meeting all testing criteria and providing an alternative option for QTP prescription.

    MeSH terms: Chemistry, Pharmaceutical/methods; Desiccation/methods; Drug Compounding/methods; Mannans/chemistry; Polymers/chemistry; Solubility; Tensile Strength; Antipsychotic Agents/administration & dosage; Antipsychotic Agents/chemistry; X-Ray Diffraction/methods; Hypromellose Derivatives/chemistry
  4. Ho C, Mohd Yusof BN, Abdul Majid H, Daud ZAM
    Clin Nutr ESPEN, 2024 Dec;64:168-176.
    PMID: 39362334 DOI: 10.1016/j.clnesp.2024.09.022
    BACKGROUND: Gynecologic cancer (GC) patients often experience systemic inflammation, malnutrition, and compromised postoperative outcomes. This systematic review aims to comprehensively synthesize existing data regarding the impact of perioperative immunonutrition (IMN) intervention on GC patients.

    METHODS: The databases of CENTRAL, EMBASE, Web of Science, and the Cochrane Library were used to conduct a literature search, supplemented by internet search engines and manual searches. Publications released between January 2009 and October 2023 was identified, reviewed, and data extracted.

    RESULTS: The review encompasses six studies involving 712 patients, comprising two randomized controlled trials (RCTs), two prospective studies, and two retrospective cohort studies. Three studies prescribed IMN perioperatively; two pre-operatively and one post-operatively. Four out of six studies reported less post-operative infection and complications. Two studies reported shorter hospitalization using the IMN formula. One study reported a longer hospitalization with IMN supplementation. Overall survival showed no significant difference in the two studies. Four studies reported positive modulation of inflammatory markers and lymphocytes as outcomes, with IMN formulas.

    DISCUSSION AND CONCLUSION: Perioperative IMN emerge as a promising intervention, demonstrating notable benefits included shortened hospitalization as well and positive modulation of inflammatory markers.

    MeSH terms: Female; Humans; Inflammation; Randomized Controlled Trials as Topic; Malnutrition
  5. Tan JP, Clyde CW, Ng CC, Yeap SK, Yong CY
    J Environ Radioact, 2024 Dec;280:107530.
    PMID: 39378736 DOI: 10.1016/j.jenvrad.2024.107530
    The global production of radioactive wastes is expected to increase in the coming years as more countries have resorted to adopting nuclear power to decrease their reliance on fossil-fuel-generated energy. Discoveries of remediation methods that can remove radionuclides from radioactive wastes, including those discharged to the environment, are therefore vital to reduce risks-upon-exposure radionuclides posed to humans and wildlife. Among various remediation approaches available, microbe-mediated radionuclide remediation have limited reviews regarding their advances. This review provides an overview of the sources and existing classification of radioactive wastes, followed by a brief introduction to existing radionuclide remediation (physical, chemical, and electrochemical) approaches. Microbe-mediated radionuclide remediation (bacterial, myco-, and phycoremediation) is then extensively discussed. Bacterial remediation involves biological processes like bioreduction, biosorption, and bioprecipitation. Bioreduction involves the reduction of water-soluble, mobile radionuclides to water-insoluble, immobile lower oxidation states by ferric iron-reducing, sulfate-reducing, and certain extremophilic bacteria, and in situ remediation has become possible by adding electron donors to contaminated waters to enrich indigenous iron- and sulfate-reducing bacteria populations. In biosorption, radionuclides are associated with functional groups on the microbial cell surface, followed by getting reduced to immobilized forms or precipitated intracellularly or extracellularly. Myco- and phycoremediation often involve processes like biosorption and bioaccumulation, where the former is influenced by pH and cell concentration. A Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis on microbial remediation is also performed. It is suggested that two research directions: genetic engineering of radiation-resistant microorganisms and co-application of microbe-mediated remediation with other remediation methods could potentially result in the discovery of in situ or ex situ microbe-involving radioactive waste remediation applications with high practicability. Finally, a comparison between the strengths and weaknesses of each approach is provided.
    MeSH terms: Bacteria/metabolism; Biodegradation, Environmental*; Radioactive Waste*
  6. de Almeida Santos G, Englund ANB, Dalleywater EL, Røhr ÅK
    FEBS Open Bio, 2024 Dec;14(12):2038-2058.
    PMID: 39382070 DOI: 10.1002/2211-5463.13906
    Tyrosinases (TYRs) are type-3 copper proteins that are widely distributed in nature. They can hydroxylate and oxidize phenolic molecules and are mostly known for producing melanins that confer protection against photo induced damage. TYRs are also thought to play an important role in the 'latch mechanism', where high concentrations of phenolic compounds inhibit oxidative decomposition of organic biomass and subsequent CO2 release, especially relevant in wetland environments. In the present study, we describe two TYRs, HcTyr1 and HcTyr2, from halophilic bacterium Hahella sp. CCB MM4 previously isolated at Matang mangrove forest in Perak, Malaysia. The structure of HcTyr1 was determined by X-ray crystallography at a resolution of 1.9 Å and represents an uncharacterized group of prokaryotic TYRs as demonstrated by a sequence similarity network analysis. The genes encoding the enzymes were cloned, expressed, purified and thoroughly characterized by biochemical methods. HcTyr1 was able to self-cleave its lid-domain (LID) in a protease independent manner, whereas the LID of HcTyr2 was essential for activity and stability. Both enzymes showed variable activity in the presence of different metals, surfactants and NaCl, and were able to oxidize lignin constituents. The high salinity tolerance of HcTyr1 indicates that the enzyme can be an efficient catalyst in the habitat of the host.
    MeSH terms: Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Bacterial Proteins/chemistry; Oxidation-Reduction*; Monophenol Monooxygenase/metabolism; Crystallography, X-Ray; Wetlands*
  7. Loke YH, Phang HC, Gobal G, Vijayaraj Kumar P, Kee PE, Widodo RT, et al.
    Drug Dev Ind Pharm, 2024 Oct;50(10):845-855.
    PMID: 39418138 DOI: 10.1080/03639045.2024.2417999
    INTRODUCTION: Fast melt tablets (FMTs) provide a convenient dosage form that rapidly dissolves on the tongue without the need for water. Cocoa butter serves as a suitable matrix system for FMTs formulation, facilitating rapid disintegration at body temperature.

    OBJECTIVES: This study aimed to formulate FMTs using cocoa butter as a base and investigate the effect of various disintegrants and superdisintegrants on their characteristics.

    METHODS: Cocoa butter-based FMTs were prepared via the fusion molding technique. Different disintegrants and superdisintegrants were added at varying concentrations and subjected to characterization. The optimal formulation was selected and incorporated with 10 mg memantine hydrochloride.

    RESULTS: The optimal FMT formulation consisted of 340 mg cocoa butter, 75 mg starch, and 75 mg crospovidone, exhibiting a hardness of 17.12 ± 0.31 N and a disintegration time of 32.67 ± 0.17 s. Furthermore, FMTs demonstrated a faster release profile compared to the commercially available product, Ebixa. SEM micrographs revealed homogenous blending of individual ingredients within the cocoa butter matrix and FT-IR analysis confirmed the chemical stability of memantine hydrochloride in the formulation. The dissolution profile of F17 suggested that the drug in FMTs released faster compared to Ebixia. Memantine hydrochloride achieved 98.07% of drug release in FMTs at 10 min. Moreover, the prepared FMTs exhibited stability for at least 6 months.

    CONCLUSION: The successful development of cocoa butter-based FMTs containing memantine hydrochloride highlights the potential of cocoa butter as viable alternative matrix-forming material for FMTs production. This innovative formulation offers patients a convenient alternative for medication administration.

    MeSH terms: Chemistry, Pharmaceutical/methods; Dietary Fats; Drug Stability; Hardness; Povidone/chemistry; Solubility; Starch/chemistry; Tablets*; Spectroscopy, Fourier Transform Infrared/methods; Drug Liberation
  8. Bamouh Z, Tifrouin I, Elkarhat Z, Abid L, Fellahi S, Elharrak M
    Microb Pathog, 2024 Dec;197:107023.
    PMID: 39423917 DOI: 10.1016/j.micpath.2024.107023
    Contagious ecthyma (CE), also known as ORF is a highly contagious zoonotic viral skin disease that affects humans, sheep, goats and other domesticated and wild animals. As reported here-in, the objective of this study was to investigate a suspected outbreak of both sheeppox and ORF diseases in a sheep herd during the winter of 2020 in Northwest Morocco. The affected sheep showed nodules and proliferative scabby skin lesions around the mouth and hairless area of the body. Samples of skin crust were collected for virus identification and isolation. A virus was isolated in Vero cells, lamb testis and heart cells and the cytopathic effect was characterized by cells aggregation, ballooning, and detachment. Initially, the suspensions of skin crust were positive for sheeppox virus (SPPV) by PCR. Subsequent testing of the isolated virus from skin crust of affected animals indicated that the virus was SPPV-negative and ORFV-positive by PCR. Furthermore, nucleotide sequences of the B2L aligned with reference ORFV isolates for genetic analysis. Phylogenetic analyses results confirmed that the isolated virus was ORFV and that the virus was closely related to ORFV strains isolated in Sudan and Malaysia. In conclusion, this study is the first reported detection of ORFV in Morocco, and therefore, poses as an imminent threat to the health of humans, domestic and wild animals.
    MeSH terms: Animals; Cercopithecus aethiops; Morocco/epidemiology; Phylogeny*; Sheep; Skin/virology; Vero Cells
  9. Sahayarayan JJ, Thiyagarajan R, Prathiviraj R, Tn K, Rajan KS, Manivannan P, et al.
    Microb Pathog, 2024 Dec;197:107028.
    PMID: 39426637 DOI: 10.1016/j.micpath.2024.107028
    The 21st century has witnessed several clinical outcomes regarding AMR. One health concept has been foreseen as a standard global public health initiative in ensuring human, animal and environmental health. The present study explores critical Gram-negative ESKAPE pathogens encompassing Acinetobacter baumannii (ACB), Klebsiella pneumoniae (KPX) and Pseudomonas aeruginosa (PAE). A comparative genomic analysis approach was utilized for identifying novel and putative genes coercing global health consequences stressing the significance of the above iatrogenic and nosocomial pathogens. O findings reveal that Pseudomonas aeruginosaPAO1 (PAE) possesses the largest genome, measuring 62,64,404 base pairs, containing 14,342 protein-coding genes and an elevated count of ORFs, surpassing other organisms. Notably, P. aeruginosa PAO1 exhibits a comprehensive metabolic landscape with 355 pathways and 1659 metabolic reactions, encompassing 200 biosynthesis and 132 degradation pathways. Transferases are the predominant enzyme category across all three genomes, followed by oxidoreductases and hydrolases. The pivotal role of beta-lactamase in conferring resistance against antibiotics is also evident in all three microbes. This investigation underscores the PAE genome harbours genes and enzymes associated with heightened virulence in antibiotic resistance. The holistic review combined with comparative genomics underlines the significance of delving into the genomes of these antimicrobial-resistant organisms. In silico methodologies are increasingly stressed in aiding the successful accomplishment of the United Nations Sustainable Development Goal -3: Good Health and Well-being. The prominent findings establish Carbapenem resistance and evolutionary lineages of the MCR-1 gene conferring AMR landscapes for future research.
    MeSH terms: Bacterial Proteins/genetics; Bacterial Proteins/metabolism; beta-Lactamases/genetics; Humans; Microbial Sensitivity Tests; Virulence/genetics; Genomics; Drug Resistance, Bacterial/genetics
  10. Miranda L, Gillett S, Ames Y, Krockenberger M, Malik R
    Aust Vet J, 2024 Dec;102(12):638-645.
    PMID: 39428359 DOI: 10.1111/avj.13375
    Sporotrichosis is caused by species of the fungus Sporothrix that affect human and several animal species. The transmission of sporotrichosis is classically from an environmental source following penetrating injury. The alternative zoonotic route has gained attention due to the rapid and significant spread of cat-transmitted sporotrichosis in South America and Malaysia, resulting in several thousand human and feline cases in recent decades, especially in Brazil. In Australia, sporotrichosis is sporadic and mainly related to environmental sources. Although feline sporotrichosis is rare in Australia, zoonotic transmission occurred in two of five reported cases, including a recent case cluster, which is reported in this article. The investigation of such cases in Australia is important, as cats not only represent a source of infection living in close proximity with humans, but also, they may represent sentinels for the environmental presence of the fungus. To encourage the investigation and reporting of new veterinary cases, we have reviewed Australian sporotrichosis cases in humans and cats and report a new case cluster of feline sporotrichosis in Perth, WA, Australia, with zoonotic transmission. The index case was a kitten who developed severe facial sporotrichosis with prominent involvement of the nasal planum. Two human cases - one suspected and one confirmed - are also described in the owner, who was a veterinary nurse, and the attending veterinarian, respectively, probably from handling the kitten while not wearing gloves. Later, another mature cat in the same household also became infected. The affected cats were successfully treated using combination of itraconazole and terbinafine. To the best of our knowledge, this is only the second case of cat-transmitted sporotrichosis reported from Australia.
    MeSH terms: Animals; Cats; Female; Humans; Male; Sporothrix/isolation & purification; Western Australia/epidemiology; Itraconazole/therapeutic use
  11. Ashok Kumar SS, Bashir S, Pershaanaa M, Kamarulazam F, Kuppusamy AV, Badawi N, et al.
    Microb Pathog, 2024 Dec;197:107059.
    PMID: 39442812 DOI: 10.1016/j.micpath.2024.107059
    In 2020, the World Health Organization (WHO) declared a pandemic due to the emergence of the coronavirus disease (COVID-19) which had resulted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). At present, the emergence of many new variants and mutants were found to be more harmful compared to the previous strains. As a result, research scientists around the world had devoted significant efforts to understand the mechanism, causes and transmission due to COVID-19 along with the treatment to cure these diseases. However, despite achieving several findings, much more was unknown and yet to be explored. Hence, along with these developments, it is also extremely essential to design effective systems by incorporating smart materials to battle the COVID-19. Therefore, several approaches have been implemented to combat against COVID-19. Recently, the graphene-based materials have been explored for the current COVID-19 and future pandemics due to its superior physicochemical properties, providing efficient nanoplatforms for optical and electrochemical sensing and diagnostic applications with high sensitivity and selectivity. Moreover, based on the photothermal effects or reactive oxygen species formation, the carbon-based nanomaterials have shown its potentiality for targeted antiviral drug delivery and the inhibitory effects against pathogenic viruses. Therefore, this review article sheds light on the recent progress and the most promising strategies related to graphene and related materials and its applications for detection, decontamination, diagnosis, and protection against COVID-19. In addition, the key challenges and future directives are discussed in detail for fundamental design and development of technologies based on graphene-based materials along with the demand aspects of graphene-based products and lastly, our personal opinions on the appropriate approaches to improve these technologies respectively.
    MeSH terms: Humans; Drug Delivery Systems; Nanostructures*; Pandemics
  12. Tse HF, Chang HY, Colquhoun D, Kim JS, Poh KK, Kostner K, et al.
    Cardiol Ther, 2024 Dec;13(4):737-760.
    PMID: 39455535 DOI: 10.1007/s40119-024-00384-3
    INTRODUCTION: Real-world data are needed to understand the effectiveness of new therapeutic options for low-density lipoprotein cholesterol (LDL-C) reduction in Asia-Pacific clinical practice. Description of evolocumab use among adults with establisHed Atherosclerotic cardiovascuLar diseasE or hypercholesterolemia in ASia-Pacific region (HALES) was performed to better understand characteristics of and clinical decision-making for adults with established atherosclerotic cardiovascular disease/hypercholesterolemia after local evolocumab approval.

    METHODS: The HALES observational study, conducted at 33 sites (Hong Kong, Thailand, South Korea, Singapore, Taiwan, and Australia) comprised (1) chart review of patients who received evolocumab, a proprotein convertase subtilisin/kexin type 9 inhibitor (PCSK9i), and (2) physician/patient survey and one-time data collection of patients with high cardiovascular risk initiating evolocumab or initiating/continuing non-PCSK9i lipid-lowering therapy. Patients could only enroll in (1) or (2).

    RESULTS: Chart review included 724 very high-risk patients initiating evolocumab from regulatory approval to 2021. From median baseline LDL-C of 3.2 mmol/L (123.7 mg/dL), patients had a median percent change in LDL-C of - 60.8% at 1-6 months. Goal achievement increased from 7.9% to 69.8% for

  13. Hamid N, Junaid M, Salim NB, Manzoor R, Chuan OM
    Food Chem Toxicol, 2024 Dec;194:115074.
    PMID: 39461501 DOI: 10.1016/j.fct.2024.115074
    Perfluorooctanoic acid (PFOA) is a long-chain legacy congener of the per- and polyfluoroalkyl substances (PFAS) family, notorious as a "forever chemical" owing to its environmental persistence and toxic nature. Essential elements such as zinc (Zn) can cause toxic effects when they change their metal speciation and become bioavailable, such as zinc sulfate (ZnSO4). Combined toxicity assessment is a realistic approach and a challenging task to evaluate chemical interactions and associated risks. Therefore, the present study aims to elucidate the acute mixture toxicity (12-48 h) of PFOA and ZnSO4 in Daphnia magna at environment-relevant concentrations (ERCs, low dose: PFOA 10 μg/L ZnSO4 20 μg/L; high dose: PFOA 20 μg/L ZnSO4 50 μg/L) in terms of developmental impact, apoptosis induction, and interaction with major endogenous antioxidants. Our results showed that deformity rates significantly increased (p 
    MeSH terms: Animals; Antioxidants*; Molecular Docking Simulation*
  14. Lo TS, Kamarudin M, Sun MJ, Su TH
    Taiwan J Obstet Gynecol, 2024 Nov;63(6):826-835.
    PMID: 39481988 DOI: 10.1016/j.tjog.2024.07.016
    Mid urethral sling (MUS) surgery is a widely accepted and safe procedure performed for stress urinary incontinence (SUI) with excellent cure rate besides its minimal complications. There are various types of MUS which can be offered. In this review we collated published data on MUS surgery performed among Taiwanese women with SUI in search for the best techniques and its outcome. We reviewed 77 articles, searched using PubMed platform related to MUS in USI among Taiwanese women from 1998 to 2023.24 articles, total 2733 participants with at least 12 months follow up after MUS. Objective cure rate for trans-obturator tape (TOT), retropubic sling (TVT, tension vaginal tape), single incision sling (SIS) (Solyx) and SIS (MiniArc) are 80%-92%, 88%-94%, 87%-90% and 87%-91% respectively, while subjective cure is 60%-90% in TOT, 86% in SIS (Solyx) and almost 90% in SIS (MiniArc), Predictors for surgical failure analyzed in 5 papers of 1006 women. Identifiable risk includes low maximal urethral closure pressure, intrinsic sphincter deficiency, previous anti SUI or prolapse surgery, presence of neurogenic disease, constipation, decreased bladder sensation, age >65 years, high pad test, Diabetes, detrusor overactivity, post-menopausal, reduced postoperative urethral mobility and tape percentile. Subsequently we dwell into complications of each type of MUS. This review showed the evolution of MUS and its comparable therapeutic efficacy. However, with certain complication rates and predictors for failure. This will add value in preoperative counselling while taking into accounts patients' factors in choosing the appropriate types of MUS. Future research is needed on long term effectiveness and risk of future recurrence.
    MeSH terms: Female; Humans; Middle Aged; Risk Factors; Urologic Surgical Procedures/methods; Taiwan; Treatment Outcome; Suburethral Slings*
  15. Rengasamy N, Othman RY, Che HS, Harikrishna JA
    Plant Physiol Biochem, 2024 Dec;217:109252.
    PMID: 39500086 DOI: 10.1016/j.plaphy.2024.109252
    Stevia rebaudiana is a high-value crop due to the strong commercial demand for its metabolites (steviol glycosides, SG) as an organic low-caloric sweetener with up to 300 times the sweetness of conventional sugar. Two experiments were conducted in this study. In the first experiment, treatments with varying green (GR1 & GR2), UVA (UV1 & UV2) and treatments that had both (UVGR1, UVGR2) were used. In the second experiment, separate set of plants were grown under base red-blue (RB) and natural sunlight before being transferred to GR2, UV2, UVGR2, and monochromatic light treatments of blue, green and UVA, for 3 and 10 days before harvest. RB and sunlight were used as the control for artificial and natural light respectively. Plants grown under the UVGR1 had the highest dry leaf biomass accumulation of 4.75 g plant-1 (P 
    MeSH terms: Glucosides/metabolism; Sunlight; Ultraviolet Rays*; Plant Leaves/metabolism; Plant Leaves/radiation effects; Biomass*; Diterpenes, Kaurane/metabolism
  16. Sidek MY, Kamarul Zaman MF, Nik Rosmawati NH, Zamzurina AB
    Med J Malaysia, 2024 Nov;79(6):661-668.
    PMID: 39614782
    INTRODUCTION: Drug-resistant tuberculosis (DR-TB) poses a serious global health threat, leading to high morbidity and mortality rates. Malaysia has witnessed an increase in DRTB cases, necessitating research into trends and characteristics. This study aims to determine the prevalence and describe the characteristics and treatment outcomes of DR-TB cases in Malaysia from 2016 to 2020.

    MATERIALS AND METHODS: A retrospective record review was carried out, utilising secondary data obtained from the TB registry of Selangor and Wilayah Persekutuan Kuala Lumpur. All registered DR-TB cases between 2016 and 2020 that met the study criteria were analysed descriptively using SPSS software version 27.

    RESULTS: Of 443 cases of registered DR-TB over 5 years, 430 cases fulfilled the study criteria. The prevalence of DR-TB increased from 0.27 to 1.79 per 100,000 population between 2016 and 2020. The average age was 40.96 years, majority were males (70.7%), Malaysian (79.3%), with Malays comprising 50.2%. Most patients had up to secondary school education (51.9%), married (57.0%), employed (53.3%) and 34.9% were smokers. For clinical characteristics, 23.5% had diabetes, and 10.9% were HIVpositive. Retreatment cases accounted for half the total, and 83.9% had positive smear results. Minimal chest X-ray lesions were observed in 54.4% of cases. The majority (66.7%) received supervised treatment from healthcare providers after being diagnosed with DR-TB, and 37.4% had more than one anti-TB resistance. Favourable treatment outcomes were observed in 56.7% of cases, while 42.1% had unfavourable outcomes, mainly due to loss to follow-up (49.7%), death (42.6%) and treatment failure (7.7%).

    CONCLUSION: The rising cases of DR-TB call for comprehensive public health interventions and stakeholder commitment to reduce its occurrence and transmission. These findings provide valuable guidance for policymakers in strengthening DR-TB control and prevention strategies.

    MeSH terms: Adolescent; Adult; Aged; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Retrospective Studies; Prevalence; Treatment Outcome; Young Adult
  17. Wan Mohamed WMM, Adam SH, Zarkasi KA, Zulkepli SZ
    Med J Malaysia, 2024 Nov;79(6):669-676.
    PMID: 39614783
    INTRODUCTION: Noise-induced hearing loss (NIHL) is the second most common form of sensorineural hearing loss. It is one of the occupational health concerns worldwide with a prevalence rate of 16%. In Malaysia, there is an increasing trend of occupational NIHL prevalence encompassing agriculture, manufacturing, transportation, and construction sectors. The Malaysian Armed Forces (MAF) personnel, particularly the marine technicians of the Royal Malaysian Navy (RMN), have a heightened risk of developing NIHL due to prolonged exposure to hazardous noise levels onboard the military vessels. Previous studies involving MAF participants recorded a prevalence rate of approximately 22%. However, limited information is available regarding occupational NIHL among the RMN marine technicians. This study aimed to determine the prevalence of occupational NIHL and its associated factors among marine technicians working on the RMN vessels.

    MATERIALS AND METHODS: A cross-sectional study was conducted among 127 randomly selected participants among marine technicians working on RMN vessels stationed at the Lumut Naval Base, Perak, Malaysia. The research instruments were questionnaires that contained information about sociodemographic, socioeconomic, occupational characteristics, and lifestyle behaviours, followed by a pure tone audiometric (PTA) assessment. Diagnosis of NIHL was made when the hearing threshold was ≥25 dB at 3 kHz to 6 kHz, with a recovery at 8 kHz on PTA.

    RESULTS: The participants' median age was 32 years (interquartile range=27-37 years). The prevalence of occupational NIHL was 29.9% (95% CI=22.1-38.7). Factors associated with occupational NIHL on unadjusted regression analysis include age >30 years (OR=2.56, p=0.0185), middle household income (OR=2.76, p=0.0227), military rank especially the warrant officer (OR=7.12, p=0.0038), and length of service ≥15 years (OR=2.40, p=0.0246). After adjusting for ethnicity, smoking status, types of vessels, and participation in noise-related leisure activities, middle household income (OR=3.15, 95% CI=1.29- 7.87, p=0.0121) and warrant officer (OR=4.38, 95% CI=1.08- 20.52, p=0.0384) remained as significant predictors for occupational NIHL in this population.

    CONCLUSION: In this study, the marine technicians working on board the RMN vessels had a higher prevalence of occupational NIHL compared to the prevalence among other MAF personnel as well as the global data. In addition, the probabilities of having occupational NIHL were significantly higher for middle-income technicians and those who ranked as warrant officers. These findings highlight the need for routine audiometric assessment and adoption of hearing conservation initiatives for individuals at high risk within this occupational cohort.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Noise, Occupational/adverse effects; Occupational Diseases/etiology; Occupational Diseases/epidemiology; Risk Factors; Ships; Prevalence; Young Adult
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