Browse publications by year: 2024

  1. Mohd-Hafifi AB, Mohamed Nor NMI, Zakaria L, Mohd MH
    Sci Rep, 2024 Nov 29;14(1):29708.
    PMID: 39613784 DOI: 10.1038/s41598-024-78195-8
    Fusarium oxysporum is a cosmopolitan fungus, consisting of both pathogenic and non-pathogenic members and known to be the causative agent of several diseases on various host plants. In Malaysia, most studies have focused on pathogenic F. oxysporum isolates because of their implications for agricultural production, but less attention has been given to non-pathogenic isolates. The aim of this study was to determine the phylogenetic relationship, genetic diversity, pathogenicity and host range of F. oxysporum in Malaysia. A total of 133 isolates of F. oxysporum were isolated from symptomatic plants of Abelmoschus esculentus, Solanum melongena, Solanum tuberosum, Cucumis sativus, Solanum lycopersicum, Cucumis melo, Musa paradisiaca var. awak, Hymenocallis littoralis, Asparagus officinalis, and Sansevieria trifasciata and non-agricultural soils in Malaysia. Comparison of nucleotide sequences of translation elongation factor 1-alpha (tef1-α) and mitochondrial small subunit (mtSSU) showed that the isolates were 98-100% similar to F. oxysporum from GenBank, thus, confirming the fungal identity. Besides, Malaysian isolates of F. oxysporum exhibited polyphyletic evolutionary origin, wide host range, and genetically diverse by grouping into 20 VCGs and 17 IGS haplotypes. This finding is beneficial for the purpose of quarantine, monitoring and disease management in the agricultural settings in Malaysia.
    MeSH terms: Malaysia; Phylogeny*; Genetic Variation*; Host Specificity
  2. Zakaria MR, Vodovnik M, Zorec M, Liew KJ, Tokiman L, Chong CS
    Antonie Van Leeuwenhoek, 2024 Nov 29;118(1):38.
    PMID: 39613983 DOI: 10.1007/s10482-024-02045-w
    The underexplored halophilic genus Joostella within the Flavobacteriaceae family consists of only two species, both of which have received little attention for their potential biotechnological applications. In this study, we report the isolation and characterisation of a novel halophilic bacterium, strain CR20, using a genomic approach to investigate its biotechnological potential. Analysis of the 16S rRNA gene revealed that strain CR20 shares 97.5% and 96.2% sequence similarity with Joostella marina DSM 19592 T and Joostella atrarenae M1-2 T, respectively. Strain CR20 exhibited average nucleotide identity and digital DNA-DNA hybridisation values of 76.8-79.1% and 20.8-22.8%, respectively, with Joostella spp., which fall below the species delineation thresholds. Additionally, strain CR20 demonstrated average amino acid identity and percentage of conserved proteins values of 81.3-84.0% and 71.7-75.3%, respectively, with Joostella spp., above the genus delineation thresholds. Meanwhile, the average amino acid identity and percentage of conserved proteins values of strain CR20 against Galbibacter spp. are 73.9-80.0% and 61.3-72.3%, respectively, also above the genus delineation thresholds. These findings indicated strain CR20 has a close relationship with both genera. Chemotaxonomic analysis of strain CR20 identified predominant fatty acids, including iso-C17:0 3OH (25.3%), iso-C15:0 (14%), and C16:1 ω6c/C16:1 ω7c (12.2%). The assembled genome comprises 62 contigs, with a size of approximately 3,168,727 bp and a G + C content of 35.1%. Among 2,804 predicted genes, 2,559 were classified into 25 COG functional groups. A total of 68 genes with potential industrial applications were identified, including 1 β-mannanase, 2 β-xylosidases, 1 polysaccharide deacetylase, 4 other hemicellulases, 6 β-glucosidases, 25 proteases, and 29 phosphate-solubilising enzymes. Hydrolytic assays confirmed that strain CR20 produces these enzymes extracellularly. These findings highlight strain CR20 has potential for industrial applications.
    MeSH terms: Base Composition; Biotechnology*; Fatty Acids/metabolism; Phylogeny*; Bacterial Typing Techniques; Genome, Bacterial*; Sequence Analysis, DNA
  3. Mishra S, Bhat D, Venkatesh MP
    Orphanet J Rare Dis, 2024 Nov 29;19(1):446.
    PMID: 39614301 DOI: 10.1186/s13023-024-03377-6
    BACKGROUND: Rare disease (RD) management and orphan drug development in India face various hurdles regarding the implementation and adoption of comprehensive policies, lack of dedicated regulatory frameworks, and absence of epidemiological data. Current rare disease policy focuses more on strengthening the diagnostics and lacks a proper comprehensive treatment framework to ensure favorable clinical outcomes. Indian patients are largely excluded from global orphan drug clinical trials. This further alienates patients from access to rare disease treatment and available treatments come at high cost. This review-based study assesses the landscape of health policies and programs in India through a review of literature and guidelines, to identify strategic opportunities and recommendations for enhancing the overall care and support for the Rare Disease (RD) patient population and improving the orphan drug research ecosystem in India.

    DISCUSSION: The absence of specific regulations, shortage of healthcare resources, budget constraints, competing health priorities, lack of patient data, and insufficient research incentives discourage orphan drug development and global clinical trial inclusion, resulting in treatment inaccessibility and high costs. The Indian Government introduced the National Policy for Treatment of Rare Diseases (NPRD) to address these challenges. Several initiatives have been introduced to attract stakeholders with government-funded research, grants, incentives, and accelerated regulatory approvals of novel therapies that can ensure timely prevention and treatment of rare diseases. The National RD Registry by the Indian Council of Medical Research (ICMR) aims to provide prevalence data. Innovative approaches are required to improve rare disease management and promote orphan drug research. This will ensure the accessibility and affordability of life-saving therapeutics for India's rare disease patients.

    CONCLUSION: An integrated RD management and orphan drug research framework focusing on robust data management, patient-oriented policies to improve the treatment landscape, flexible regulations, strengthening rare disease registry with clinical and diagnostic data, and a favorable research ecosystem to promote indigenous research catering to the Indian population, will improve the treatment landscape and orphan drug research and development in India. This will ensure timely availability of therapeutics at affordable prices.

    MeSH terms: Health Policy*; Humans; India; Orphan Drug Production*
  4. Adnan MAA, Sahril N, Abd Razak MA, Shamsuddin N, Hasim Hashim MH, Abdul Mutalip MH
    J Health Popul Nutr, 2024 Nov 29;43(1):200.
    PMID: 39614324 DOI: 10.1186/s41043-024-00689-y
    BACKGROUND: Inadequate knowledge of human immunodeficiency virus (HIV) is underscored as a key factor contributing to the HIV epidemic. Nonetheless, current data on HIV knowledge in Malaysia are still scarce. Therefore, this study aimed to assess the prevalence of inadequate HIV knowledge and its associated factors among the general Malaysian population.

    METHOD: The study utilized data from the National Health and Morbidity Survey (NHMS) 2020, a nationwide cross-sectional survey employing a two-stage stratified random sampling technique to ensure national representativeness. HIV knowledge was assessed using the UNGASS indicators questionnaire, which comprises five questions on HIV prevention and transmission. The data were collected using the computer assisted telephone interviewing (CATI) method. Respondents who did not correctly answer all five questions were considered to have inadequate knowledge about HIV. Descriptive analysis and complex sample logistic regression were performed using SPSS version 28.0.

    RESULTS: The survey identified 5,561 eligible respondents, leading to the participation of 3,187 individuals in the second phase of the study, which resulted in a response rate of 57.3%. Our study revealed an overall prevalence of inadequate HIV knowledge at 77.4%, with adolescents aged 13-19 exhibiting the highest prevalence at 86.1%. Multiple logistic regression analysis indicated that respondents with no formal education (aOR 4.34, 95% CI: 0.65, 29.08) were over four times more likely to lack HIV knowledge. Additionally, respondents with only secondary education had an increased risk of 1.79 times. Individuals residing in rural areas were significantly more likely to have inadequate HIV-related knowledge. Furthermore, respondents who worked as unpaid workers, homemakers, or caregivers (aOR 1.71, 95% CI: 1.05, 2.82) showed a higher likelihood of lacking HIV knowledge.

    CONCLUSION: Three out of four individuals in the general Malaysian population were found to lack sufficient knowledge about HIV. This underscores the need for targeted interventions in HIV education, particularly in rural areas and among populations with lower educational attainment. Additionally, digital platforms and youth-focused campaigns could be especially effective for reaching adolescents. Policymakers must prioritize inclusive, accessible HIV prevention strategies to address these gaps and reduce transmission rates.

    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Female; Health Surveys*; Humans; Health Knowledge, Attitudes, Practice*; Malaysia/epidemiology; Male; Middle Aged; Rural Population/statistics & numerical data; Prevalence; Young Adult
  5. Ashhar Z, Ahmad Fadzil MF, Hassan H, Othman MF, Md Hassan MB, Chun Vui VY, et al.
    Curr Med Imaging, 2024;20:e15734056270935.
    PMID: 38874043 DOI: 10.2174/0115734056270935231113035620
    Skeletal-related events due to bone metastases can be prevented by early diagnosis using radiological or nuclear imaging techniques. Nuclear medicine techniques such as Single Photon Emission Computed Tomography (SPECT) and Positron Emission Tomography (PET) have been used for diagnostic imaging of bone for decades. Although it is widely recognized that conventional diagnostic imaging techniques such as Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) have high sensitivity, low cost and wide availability, the specificity of both techniques is rather low compared to nuclear medicine techniques. Nuclear medicine techniques, on the other hand, have improved specificity when introduced as a hybrid imaging modality, as they can combine physiological and anatomical information. Two main radiopharmaceuticals are used in nuclear medicine: [99mTc]-methyl diphosphonate ([99mTc]Tc-MDP) from the generator and [18F]sodium fluoride ([18F]NaF) from the cyclotron. The former is used in SPECT imaging, while the latter is used in PET imaging. However, recent studies show that the role of radiolabeled bisphosphonates with gallium-68 (68Ga) and fluorine-18 (18F) may have a potential role in the future. This review, therefore, presents and discusses the brief method for producing current and future potential radiopharmaceuticals for bone metastases.
    MeSH terms: Diphosphonates*; Gallium Radioisotopes; Humans; Technetium Tc 99m Medronate; Radiopharmaceuticals*
  6. Zhang H, Targher G, Byrne CD, Kim SU, Wong VW, Valenti L, et al.
    Hepatol Int, 2024 Aug;18(4):1178-1201.
    PMID: 38878111 DOI: 10.1007/s12072-024-10702-5
    BACKGROUND: With the implementation of the 11th edition of the International Classification of Diseases (ICD-11) and the publication of the metabolic dysfunction-associated fatty liver disease (MAFLD) nomenclature in 2020, it is important to establish consensus for the coding of MAFLD in ICD-11. This will inform subsequent revisions of ICD-11.

    METHODS: Using the Qualtrics XM and WJX platforms, questionnaires were sent online to MAFLD-ICD-11 coding collaborators, authors of papers, and relevant association members.

    RESULTS: A total of 890 international experts in various fields from 61 countries responded to the survey. We also achieved full coverage of provincial-level administrative regions in China. 77.1% of respondents agreed that MAFLD should be represented in ICD-11 by updating NAFLD, with no significant regional differences (77.3% in Asia and 76.6% in non-Asia, p = 0.819). Over 80% of respondents agreed or somewhat agreed with the need to assign specific codes for progressive stages of MAFLD (i.e. steatohepatitis) (92.2%), MAFLD combined with comorbidities (84.1%), or MAFLD subtypes (i.e., lean, overweight/obese, and diabetic) (86.1%).

    CONCLUSIONS: This global survey by a collaborative panel of clinical, coding, health management and policy experts, indicates agreement that MAFLD should be coded in ICD-11. The data serves as a foundation for corresponding adjustments in the ICD-11 revision.

    MeSH terms: Humans; Surveys and Questionnaires; Global Health; International Classification of Diseases*
  7. Hassan R, Mutalib AA, Shang CY, Sachdev NS, Rahman FA, Ling ESL
    J Interv Card Electrophysiol, 2024 Nov;67(8):1735-1743.
    PMID: 39110272 DOI: 10.1007/s10840-024-01884-x
    BACKGROUND: While studies comparing the effectiveness of remifentanil and dexmedetomidine are prevalent in other nations, using remifentanil alone is uncommon in Malaysia. This research aims to evaluate the effectiveness of sedation with remifentanil or dexmedetomidine infusion in monitored anesthesia care for electrophysiology procedures.

    METHODS: This study is a single-center, single-blinded, prospective randomized clinical study. One hundred twenty patients were randomized into two groups (remifentanil vs dexmedetomidine). Demographic characteristics and clinical outcomes, including level of sedation, vital signs, and patient satisfaction were monitored and recorded.

    RESULTS: Group R showed a higher mean observer's assessment of alertness/sedation score (3.9 ± 0.7 vs 3.6 ± 0.8; p = 0.008), mean arterial pressure (92.0 ± 12.0 vs 83.0 ± 13.0 mmHg; p 

    MeSH terms: Adult; Female; Humans; Infusions, Intravenous; Male; Middle Aged; Piperidines/administration & dosage; Prospective Studies; Single-Blind Method; Treatment Outcome; Patient Satisfaction; Electrophysiologic Techniques, Cardiac
  8. Jothee S, Ahmad NAB, Zainun KA, Byard RW
    Acta Paediatr, 2024 Nov 29.
    PMID: 39611530 DOI: 10.1111/apa.17525
  9. Hasan S, Chew KS
    Asian Pac J Cancer Prev, 2024 Nov 01;25(11):3781-3788.
    PMID: 39611900 DOI: 10.31557/APJCP.2024.25.11.3781
    BACKGROUND: The diagnosis and treatment of breast cancer are very distressing for young women and can result in a lot of unmet psychosocial needs and a lower quality of life.

    OBJECTIVE: Although unmet psychosocial needs in young women with breast cancer can negatively impact their quality of life, these needs have not been systematically reviewed. The primary objective of this scoping review was to identify the categories of unmet psychosocial needs among young women with breast cancer. The secondary objective was to examine the relationship between these unmet psychosocial needs and their quality of life.

    METHODS: Five electronic databases (MEDLINE from the National Library of Medicine, Cumulative Index of Nursing and Allied Health Literature (CINAHL), SCOPUS, Web of Science WOS, and Google Scholar), as well as reference lists of relevant literature were systematically searched to identify the relevant literature following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement for reporting systematic reviews.

    RESULTS: From an initial yield of 2505 articles, 30 articles were included and analyzed. The results identified 13 main domains of unmet psychosocial needs among young women with breast cancer: information needs, psychological needs, physical/symptoms needs, fear of recurrence and spreading, identity-related needs, social needs, sexual needs, social support, financial support, practical needs, spiritual needs, communication-related issues with health care providers, and coping needs. Data were extracted and summarized in a narrative synthesis.

    CONCLUSION: Ongoing assessment of informational needs and a clear understanding of the relationship between unmet psychosocial needs and quality of life are essential for healthcare providers to develop robust support systems for young women with breast cancer.

    MeSH terms: Adult; Female; Health Services Needs and Demand; Humans; Quality of Life*; Social Support*; Needs Assessment
  10. Rahim S, Isa MH, Shams S, Jeludin M, Abdul Rahman EK, Hj Md Juani R, et al.
    Environ Sci Pollut Res Int, 2024 Dec;31(58):66029-66043.
    PMID: 39612092 DOI: 10.1007/s11356-024-35580-0
    A landfill biocover is essential for addressing environmental concerns, especially in waste management, as it plays a crucial role in mitigating the release of methane gas. This study investigates the geotechnical characteristics of soil amended with organic wastes for landfill biocover applications. Various organic waste amendments, viz., rice husk, crushed coconut coir, and compost, were examined at different percentages (0%, 25%, 50%, and 75%) compared with conventional landfill cover material, i.e. natural clay, as biocovers. Laboratory experiments analysed geotechnical characteristics, including organic content, Atterberg limit, compaction, consolidation, and desiccation cracking. The study revealed that organic waste amendment significantly impacted the geotechnical characteristics of landfill biocover, enhancing organic content and porosity and reducing permeability and desiccation susceptibility. Soils amended with organic content support methanotrophic bacteria growth and reduce methane emissions in landfills. The most promising biocovers were identified as 75CR (crushed coconut coir/wastewater sludge/clay in percentage ratio of 70:5:25), followed by 75CT (compost/wastewater sludge/clay in percentage ratio of 70:5:25), and 25RH (rice husk/wastewater sludge/clay in percentage ratio of 20:5:75). Biocovers offer sustainable landfill alternatives, underscoring the need to understand their geotechnical characteristics for successful installation in landfills.
    MeSH terms: Methane/analysis; Refuse Disposal; Waste Management/methods; Waste Disposal Facilities*
  11. Berohan ND, Alias H, Ishak S, Mohammed Nawi A, Azman N, Sohaimi D, et al.
    Arch Gynecol Obstet, 2024 Oct;310(4):1959-1965.
    PMID: 39110209 DOI: 10.1007/s00404-024-07661-5
    PURPOSE: There is no standardized best method on monitoring of patients with gestational diabetes on diet modification in the country. This study aims to investigate the optimum method of self-monitoring blood glucose.

    METHODS: This is a randomized clinical trial in a single tertiary centre involving patients with gestational diabetes mellitus (GDM) diagnosed based on NICE guideline on diet modification. The patients are randomized in 1:1 ratio to 4 or 7 points self-monitoring blood glucose. The monitoring was required to be done monthly with ultrasound for fetal growth. Blood was taken at recruitment for measurement of serum HbA1c and fructosamine.

    RESULTS: A total of 200 patients were recruited. There were significantly more Malay patients in the 7 points group (88.9% vs 78.2%, p = 0.033). Multiparous patients were significantly more in the 4 points group (82.2% vs 68.7%, p = 0.033). Both groups were similar in clinical characteristics. There was no statistical difference in the neonatal outcome particularly fetal macrosomia and admission to neonatal intensive care unit.

    CONCLUSIONS: In patients with GDM on diet modification, self-blood glucose monitoring using either 4 or 7 points resulted in similar maternal and perinatal outcomes. The research was registered under ClinicalTrials.gov (NCT04101396) on 17/9/2019 ( https://register.

    CLINICALTRIALS: gov/prs/app/action/SelectProtocol?sid=S00098EN&selectaction=Edit&uid=U0004RD4&ts=2&cx=-qlk1w2 ).

    MeSH terms: Adult; Blood Glucose/analysis; Blood Glucose/metabolism; Female; Fetal Macrosomia/prevention & control; Humans; Infant, Newborn; Malaysia; Pregnancy; Blood Glucose Self-Monitoring*; Fructosamine/blood; Tertiary Care Centers
  12. Kc B, Alrasheedy AA, Mohamed Ibrahim MI, Paudyal V, Christopher CM, Shrestha S, et al.
    Pain Manag, 2024 Sep;14(9):519-529.
    PMID: 39439259 DOI: 10.1080/17581869.2024.2411930
    Aim: To examine the range of services pharmacists provide and their impact on patient outcomes, harm reduction, and appropriate opioid use.Methods: Six databases were searched (MEDLINE, EMBASE, Scopus, PsycINFO, CENTRAL and Cochrane Methodology Register) from inception to March 2023. The protocol was registered in PROSPERO (CRD42023401895).Results: Twenty-nine studies identified five key areas of pharmacist interventions in opioid management-naloxone programs and opioid de-escalation, patient and primary healthcare providers' education and motivational interview, prescription monitoring and opioid risk screening, clinical pharmacy interventions (pharmacotherapy, medication review, prescribing, adherence monitoring), and collaborative healthcare approaches to promote optimal opioid use. Outcomes assessment indicated harm reduction, improved safety, increased non-opioid analgesic use, decreased opioid consumption, and enhanced pain management.Conclusion: This review underscores pharmacists' vital role in tackling opioid misuse, overuse and abuse, providing a foundation for evidence-based policies to minimize harm and promote optimal opioid use.
    MeSH terms: Humans; Pharmacists*; Professional Role; Pain Management/methods; Prescription Drug Misuse/prevention & control; Prescription Drug Misuse/statistics & numerical data
  13. Jongsoowiwatwong N, Flaherty GT, Pisutsan P, Matsee W, Kusolsuk T, Silachamroon U, et al.
    Travel Med Infect Dis, 2024;62:102780.
    PMID: 39447943 DOI: 10.1016/j.tmaid.2024.102780
    BACKGROUND: Last-minute travelers (LMTs) present significant challenges for travel health services and are considered vulnerable due to their lack of health preparation. However, there is a lack of data to support this assumption.

    METHODS: The proportion of LMTs was investigated through a cross-sectional study involving all Thai travelers who visited the Thai Travel Clinic before their departure abroad. A prospective study was conducted by enrolling the travelers after the consultation, utilizing two online questionnaires. The first aimed to gather demographic data and categorize participants as either LMTs (if their departure date was ≤14 days) or non-LMTs, while the second assessed travel-related illnesses either upon their return or at the one-month point if their trip exceeded a month.

    RESULTS: A quarter (25.5 %) of 310 Thai travelers abroad were classified as LMTs. Both LMTs and non-LMTs showed similar gender distributions with mean ages of 35.8 and 35.7 years old, respectively, but LMTs were more likely to travel for tourism, travel in groups, visit countries within Asia and plan shorter stays abroad. Follow-up studies were conducted from July 2023 to February 2024. 452 departed respondents consisted of 150 LMTs and 302 non-LMTs. Although overall health problems were insignificantly higher in LMTs (32.0 % vs 22.0 %, AOR = 1.469, p = 0.107), gastrointestinal and neurological symptoms (primarily headache and dizziness) were significantly more common among LMTs.

    CONCLUSIONS: LMTs represent a significant portion of Thai travelers, posing challenges for travel health specialists in Thailand. Intervention and education efforts may be necessary to address this issue.

    MeSH terms: Adult; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Morbidity; Prospective Studies; Surveys and Questionnaires; Thailand/epidemiology; Time Factors; Young Adult; Travel Medicine/statistics & numerical data
  14. Solangi NH, Karri RR, Mubarak NM, Mazari SA, Sharma BP
    Nanoscale, 2024 Nov 28;16(46):21216-21263.
    PMID: 39470605 DOI: 10.1039/d4nr03008g
    Essential biosensor use has become increasingly important in drug discovery and recognition, biomedicine, food safety, security, and environmental research. It directly contributed to the development of specialized, reliable diagnostic instruments known as biosensors, which use biological sensing components. Traditional biosensors have poor performance, so scientists need to develop advanced biosensors with promising selectivity, sensitivity, stability, and reusability. These are all parameter modifications associated with the characteristics of the sensing material. Carbon nanotubes (CNTs) and MXenes are promising as targeted sensing agents in advanced functional materials because of their promising chemical and physical properties and limited toxic effects. Based on available data and sensing performance, MXene is better for biosensing applications than CNTs. Because of their large specific surface area (SSA), superior electrical conductivity, and adaptable surface chemistry that facilitates simple functionalization and robust interactions with biomolecules, MXenes are typically regarded as the superior option for biosensors. Additionally, because of their hydrophilic nature, they are more suited to biological settings, which increases their sensitivity and efficacy in identifying biological targets. MXenes are more suitable for biosensing applications due to their versatility and compatibility with aquatic environments, even if CNTs have demonstrated stability and muscular mechanical strength. However, MXenes offer better thermal stability, which is crucial for applications in diverse temperature environments. This study reviews and compares the biosensing capabilities, synthesis methods, unique properties, and toxicity of CNTs and MXenes. Both nanomaterials effectively detect various pollutants in food, biological substances, and human bodies, making them invaluable in environmental monitoring and medical diagnostics. In conclusion, CNTs work better for biosensors that must be strong, flexible, and long-lasting under different conditions. MXenes, on the other hand, work better when chemical flexibility and compatibility with wet environments are essential.
    MeSH terms: Humans; Biosensing Techniques*
  15. Obaidur Rab S, Altalbawy FMA, Chandra M, Ariffin IA, Kaur P, Rathore G, et al.
    Pathol Res Pract, 2024 Dec;264:155679.
    PMID: 39500198 DOI: 10.1016/j.prp.2024.155679
    Lung malignancies are among the most prevalent and foremost causes of tumor-related deaths. Despite significant advancements in the understanding and management of lung cancer, resistance to traditional treatments remains a significant challenge. Understanding and targeting tumor microenvironment (TME) have attracted interest in the recent decade for eliminating various solid tumors. The lung TME has a crucial position in tumor expansion and therapy failure, driving it an engaging target for novel medicinal interventions. Plant-derived products offer a promising avenue for targeting TME due to their diverse chemical structures and biological activities. However, their clinical use is hindered by insufficient bioavailability and also possible systemic toxicity. The use of nanoparticles as delivery vehicles for natural products can overcome these challenges and enhance their therapeutic efficacy. This review article explores the potential of plant-derived products as medicinal agents for targeting lung TME. We provide an outline of the present knowledge of lung TME and explain the mechanisms by which plant-derived products can modulate key components of this microenvironment. The promising impacts and properties of nanoparticles for the delivery of these derivatives into lung tumors will also be discussed. We also review the preclinical and clinical findings for supporting the usefulness of these agents in targeting lung TME. Additionally, we highlight the challenges and forthcoming trends in the development of plant-derived products as targeted therapies for lung cancer, with a particular focus on combination therapies.
    MeSH terms: Animals; Antineoplastic Agents, Phytogenic/pharmacology; Antineoplastic Agents, Phytogenic/therapeutic use; Humans; Nanoparticles*
  16. Zhang Q, Lee K, Qian P, Mansor Z, Ismail I, Guo Y, et al.
    J Adv Nurs, 2024 Nov 28.
    PMID: 39607180 DOI: 10.1111/jan.16541
    AIMS: To investigate the prevalence of rapid response team delays, survival distribution of admission to rapid response team delay and its prognostic factors.

    DESIGN: A retrospective single-centre study.

    METHODS: Data on rapid response team activations from 1 January 2018 to 31 December 2022 were retrieved from electronic medical records at a tertiary hospital in Hangzhou, China. All patients who met the eligibility criteria were included. Multivariable Cox regression analysis was conducted to analyse the data.

    RESULTS: Out of 636 patients included, 18.4% (117) experienced a delay, with a median (interquartile range) of 8.5 (12) days from admission to rapid response team activation. Six significant prognostic factors were found to be associated with the higher hazard ratio of rapid response team delay, including call time (05:01 PM and 7:59 AM), emergency admission, a higher Modified Early Warning Score, an admission diagnosis of infection, a comorbidity of respiratory failure/Acute Respiratory Distress Syndrome and the absence of lung infection.

    CONCLUSION: The prevalence of rapid response team delays was lower, and the days from admission to rapid response team delay was longer than in previous studies. Healthcare providers are suggested to prioritise the care of high-risk patient groups and provide proactive monitoring to ensure timely identification and management.

    IMPLICATIONS FOR PATIENT CARE: Implementing artificial intelligence in continuous monitoring systems for high-risk patients is recommended. The findings help nurses anticipate potential delays in rapid response team activation, enabling better preparedness.

    IMPACT: The study highlights the prevalence of rapid response team delays, timing from admission to rapid response team activation and six prognostic factors influencing delays. It could shape patient care and inform future research. Hospital administrators should review staffing, especially during night shifts, to minimise delays. Further qualitative research is needed to explore why nurses may delay rapid response team activation.

    REPORTING METHOD: The STROBE checklist was adhered to when reporting this study. 'No patient or public contribution'.

  17. Lim WY, Lau EV, Ramakrishnan N
    Anal Chem, 2024 Dec 10;96(49):19213-19219.
    PMID: 39607411 DOI: 10.1021/acs.analchem.4c05466
    We report a Technical Note on detecting nanoplastics in water samples through electrophoresis and quartz crystal microbalance (QCM) instrumentation. We conducted electrophoresis experiments by immersing a QCM in a sample of ultrapure water containing polyethylene (PE) nanoplastics. It was interesting to observe that nanoplastics were attracted toward the QCM and adhered to one side of the QCM electrode. The attached particles introduced mass loading to the QCM and were characterized by a decrease in resonance frequency of the crystal. Furthermore, when a small region around the center of electrode was alone exposed for direct contact in water and the rest of the electrode was masked using photoresist, the nanoplastics were concentrated only in the exposed electrode region, significantly enhancing detection sensitivity. To further investigate the applicability for real-life water samples, we experimented with the technique with readily available bottled drinking water and mineral water, where we spiked these water samples with nanoplastics. It was observed that the resonance frequency shifts were significantly larger for samples with nanoplastics compared to samples without nanoplastics. In addition, Raman spectroscopy and microscopy imaging were used to further confirm the presence and locations of nanoplastics on the electrode surface. This study highlights the combination of electrophoresis and QCM effectiveness in detecting nanoplastics across different water types and their potential for broader applications in environmental monitoring.
  18. Sheeran N, Jones L, Corbin B, Melville C
    Aust J Prim Health, 2024 Nov;30.
    PMID: 39607817 DOI: 10.1071/PY24100
    Background Abortion care is typically undertaken by doctors; however, alternate models, including nurse-led care, are increasingly seen as viable alternatives. However, attitudes towards the leadership of alternate models can be a barrier to change. We explored the acceptability of different models of abortion care, and whether attitudes differed by health profession for those working in sexual and reproductive health. Methods Our mixed method survey explored how doctors, nurses/midwives and those working in administrative roles in primary care in Australia felt about three models of abortion care: doctor-led, nurse-led and self-administered. ANOVAs compared favourability ratings and attitude strength across groups, and qualitative data exploring how they felt about each model was thematically analysed using Leximancer. Results Attitudes towards doctor-led and nurse-led models of care were overwhelmingly positive. However, doctors perceived doctor-led care more favourably than other professionals, and felt it provides a more holistic, safer experience, that opportunistically facilitated discussions about other sexual and reproductive health matters. Self-administered care was perceived unfavourably by ~60% of participants, and was associated with significant safety concerns. Conclusions Most health professionals working in sexual and reproductive health care perceive that nurse-led models of care are viable and acceptable, although doctors feel there are additional benefits to the current model. Self-administered abortion is overwhelmingly perceived as unsafe. Nurse-led care models could increase access to safe abortion in Australia, and are perceived favourably by those working in sexual and reproductive health care.
    MeSH terms: Adult; Attitude of Health Personnel*; Australia; Female; Health Personnel/psychology; Humans; Male; Middle Aged; Physicians/psychology; Pregnancy; Surveys and Questionnaires; Reproductive Health Services; Reproductive Health*
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