Browse publications by year: 2024

  1. Jamaludin NI, Sahabuddin FNA, Hanafi H, Shaharudin S
    J Bodyw Mov Ther, 2024 Oct;40:1238-1242.
    PMID: 39593440 DOI: 10.1016/j.jbmt.2024.07.045
    BACKGROUND: Single leg squat (SLS) is an important motion commonly observed in sports. Excessive dynamic knee valgus (DKV) during SLS may increase non-contact knee injury risks particularly in physically active females. As muscular strength plays crucial role in controlling DKV, the present study aims to evaluate the correlation between hip strength and knee frontal plane angle during SLS among females with and without excessive DKV.

    METHODS: Thirty-four volunteers were allocated to the valgus (n = 17) and non-valgus (n = 17) groups. Their motions during SLS at 45° and 60° knee flexion were captured and analyzed using three-dimensional motion analysis system. Isokinetic hip strength was examined at 180°/s in flexion, extension, abduction, and adduction for both legs. Pearson's correlation test was computed to evaluate the relationship between hip strength and knee angle during SLS.

    FINDINGS: Non-dominant hip extensor strength (r = -0.56, p = 0.02) and dominant hip adductor strength (r = -0.51, p = 0.04) were significantly related to the knee frontal plane angle during 45° SLS among those without DKV. Meanwhile, those with DKV showed a significant relationship between the knee frontal plane angle for both legs and non-dominant hip abductor strength during 60° SLS.

    INTERPRETATION: Both groups demonstrated the relationship of hip strength on knee frontal plane angle during SLS, whereby increased hip strength may minimize excessive DKV. Those with excessive DKV should train to strengthen their hip abductor to reduce knee valgus particularly during deep squats.

    MeSH terms: Adult; Biomechanical Phenomena/physiology; Female; Hip/physiology; Hip Joint/physiology; Humans; Knee/physiology; Movement/physiology; Muscle, Skeletal/physiology; Young Adult
  2. Shetty S, Maiya GA, Rao Kg M, Vijayan S, George BM
    J Bodyw Mov Ther, 2024 Oct;40:1588-1604.
    PMID: 39593495 DOI: 10.1016/j.jbmt.2024.08.013
    OBJECTIVE: To systematically review, summarize and appraise evidence on the factors determining quality of life (QoL) after total knee arthroplasty (TKA) in individuals with knee osteoarthritis.

    METHODS: We searched six databases (PubMed, Scopus, Web of Science, CINAHL, EMBASE, and ProQuest) using appropriate search terms to identify the relevant literature published on the factors determining QoL following TKA. Two reviewers independently performed the study screening and study selection. A third reviewer was consulted in case of any disagreement. The methodological quality of the included studies was assessed using the Modified Downs and Black Index checklist. This review was registered in PROSPERO (CRD42022352887) and reported according to the PRISMA checklist.

    RESULTS: We identified a total of 8517 studies, 29 of which were included. Advanced age; female sex; increased body mass index (BMI); the presence of comorbidities such as diabetes; contralateral knee pain; poor preoperative status; psychological and pain-related factors such as the presence of pain catastrophizing; central sensitization; kinesiophobia; anxiety; depression; chronic pain; psychological distress; low level of optimism; and reduced patient satisfaction were used to determine post-TKA QoL scores. High BMI and depression were the most common factors evaluated in these studies. Overall, the methodological quality of the included studies varied from high to low.

    CONCLUSION: After TKA, the overall QoL score improved. However, there are a few physical, behavioral, and psychological factors that influence QoL. Identifying these factors could aid clinicians and health professionals in treating and rehabilitating patients by helping them improve patient prognosis after TKA.

    MeSH terms: Age Factors; Anxiety; Depression/psychology; Female; Humans; Male; Quality of Life*; Sex Factors; Body Mass Index; Patient Satisfaction; Catastrophization/psychology
  3. Noor KAM, Norsuddin NM, Karim MKA, Isa INC, Ulaganathan V
    Diagnostics (Basel), 2024 Nov 15;14(22).
    PMID: 39594234 DOI: 10.3390/diagnostics14222568
    BACKGROUND/OBJECTIVE: This study evaluates the mean glandular dose (MGD) in mammography screening for women aged 40-69 in Dubai, based on a retrospective analysis of a dose survey involving 2599 participants.

    METHODS: MGD was calculated using the Dance formula.

    RESULTS: The average MGD was 0.96 ± 0.39 mGy for mediolateral oblique (MLO) views and 0.81 ± 0.33 mGy for craniocaudal (CC) views. Weak inverse correlations were found between age and organ dose (OD) for both views, while a direct relationship was observed between breast thickness and entrance skin dose (ESD). In adjusted models, ESD was strongly associated with MGD (β = 1.04, 95% CI: 0.97, 1.09), while OD showed a moderate association (β = 0.44, 95% CI: 0.40, 0.49). Significant variations in ESD, OD, and MGD were noted across age groups and breast thicknesses.

    CONCLUSIONS: Lower MGD indicates reduced radiation exposure risk, while higher MGD in MLO views suggests improved imaging quality. Monitoring and optimizing MGD are essential for enhancing patient safety and screening efficacy.

  4. Rehman ZU, Ahmad Fauzi MF, Wan Ahmad WSHM, Abas FS, Cheah PL, Chiew SF, et al.
    Cancers (Basel), 2024 Nov 11;16(22).
    PMID: 39594748 DOI: 10.3390/cancers16223794
    Fluorescence in situ hybridization (FISH) is widely regarded as the gold standard for evaluating human epidermal growth factor receptor 2 (HER2) status in breast cancer; however, it poses challenges such as the need for specialized training and issues related to signal degradation from dye quenching. Silver-enhanced in situ hybridization (SISH) serves as an automated alternative, employing permanent staining suitable for bright-field microscopy. Determining HER2 status involves distinguishing between "Amplified" and "Non-Amplified" regions by assessing HER2 and centromere 17 (CEN17) signals in SISH-stained slides. This study is the first to leverage deep learning for classifying Normal, Amplified, and Non-Amplified regions within HER2-SISH whole slide images (WSIs), which are notably more complex to analyze compared to hematoxylin and eosin (H&E)-stained slides. Our proposed approach consists of a two-stage process: first, we evaluate deep-learning models on annotated image regions, and then we apply the most effective model to WSIs for regional identification and localization. Subsequently, pseudo-color maps representing each class are overlaid, and the WSIs are reconstructed with these mapped regions. Using a private dataset of HER2-SISH breast cancer slides digitized at 40× magnification, we achieved a patch-level classification accuracy of 99.9% and a generalization accuracy of 78.8% by applying transfer learning with a Vision Transformer (ViT) model. The robustness of the model was further evaluated through k-fold cross-validation, yielding an average performance accuracy of 98%, with metrics reported alongside 95% confidence intervals to ensure statistical reliability. This method shows significant promise for clinical applications, particularly in assessing HER2 expression status in HER2-SISH histopathology images. It provides an automated solution that can aid pathologists in efficiently identifying HER2-amplified regions, thus enhancing diagnostic outcomes for breast cancer treatment.
  5. Mansor NI, Balqis TN, Lani MN, Lye KL, Nor Muhammad NA, Ismail WIW, et al.
    Int J Mol Sci, 2024 Nov 06;25(22).
    PMID: 39595973 DOI: 10.3390/ijms252211904
    Despite significant improvements in the comprehension of neuro-regeneration, restoring nerve injury in humans continues to pose a substantial therapeutic difficulty. In the peripheral nervous system (PNS), the nerve regeneration process after injury relies on Schwann cells. These cells play a crucial role in regulating and releasing different extracellular matrix proteins, including laminin and fibronectin, which are essential for facilitating nerve regeneration. However, during regeneration, the nerve is required to regenerate for a long distance and, subsequently, loses its capacity to facilitate regeneration during this progression. Meanwhile, it has been noted that nerve regeneration has limited capabilities in the central nervous system (CNS) compared to in the PNS. The CNS contains factors that impede the regeneration of axons following injury to the axons. The presence of glial scar formation results from this unfavourable condition, where glial cells accumulate at the injury site, generating a physical and chemical barrier that hinders the regeneration of neurons. In contrast to humans, several species, such as axolotls, polychaetes, and planarians, possess the ability to regenerate their neural systems following amputation. This ability is based on the vast amount of pluripotent stem cells that have the remarkable capacity to differentiate and develop into any cell within their body. Although humans also possess these cells, their numbers are extremely limited. Examining the molecular pathways exhibited by these organisms has the potential to offer a foundational understanding of the human regeneration process. This review provides a concise overview of the molecular pathways involved in axolotl, polychaete, and planarian neuro-regeneration. It has the potential to offer a new perspective on therapeutic approaches for neuro-regeneration in humans.
    MeSH terms: Ambystoma mexicanum/physiology; Animals; Humans; Nerve Regeneration*; Signal Transduction
  6. Wong RSM, Ho Jang J, Wong LLL, Kim JS, Rojnuckarin P, Goh YT, et al.
    Int J Mol Sci, 2024 Nov 13;25(22).
    PMID: 39596227 DOI: 10.3390/ijms252212160
    Paroxysmal nocturnal hemoglobinuria (PNH) clones can be identified in a significant proportion of patients with aplastic anemia (AA). Screening for PNH clones at the time of an AA diagnosis is recommended by national and international guidelines. In this report, an expert panel of physicians discusses current best practices and provides recommendations for managing PNH in patients with AA in the Asia-Pacific region. Plasma/serum lactate dehydrogenase (LDH) levels and reticulocyte count should be measured with every blood test. PNH clone size should be monitored regularly by flow cytometry, with on-demand testing in the event of a rise in LDH level ± reticulocyte count or development of symptoms such as thrombosis. Monitoring for PNH clones can guide the choice of initial AA treatment, although flow cytometry has resource implications which may present a challenge in some Asia-Pacific countries. The treatment of patients with both PNH and AA depends on which condition predominates; following PNH treatment guidelines if hemolysis is the main symptom and AA treatment guidelines if bone marrow failure is severe (regardless of whether hemolysis is mild or moderate). The expert panel's recommendations on the monitoring and treatment of PNH in patients with AA are practical for healthcare systems in the Asia-Pacific region.
    MeSH terms: Asia/epidemiology; Flow Cytometry; Humans; L-Lactate Dehydrogenase/blood; Practice Guidelines as Topic; Reticulocyte Count; Consensus
  7. Elias MH, Abdul Hamid N, Maluin SM, Aris S, Kadiman S, Muhammad Hafidz K, et al.
    Int J Mol Sci, 2024 Nov 14;25(22).
    PMID: 39596303 DOI: 10.3390/ijms252212238
    The inability to accurately predict the occurrence of postoperative cognitive dysfunction (POCD) among open-heart surgery patients leads to concerning increases in POCD cases. Preoperative circulating biomarkers are important to identify as they are non-invasive and could provide an early prediction of POCD development, allowing for earlier and more strategized interventions. However, to date, no robust circulating biomarkers have proven effective for preoperative POCD prediction. This systematic review aims to synthesize current evidence on preoperative protein profiling among POCD patients following open-heart surgery. Thus, a thorough literature search employing PubMed, EBSCOhost, Scopus, and Science Direct was carried out. This combination of keywords was used as part of the search strategy: ("Postoperative cognitive decline" OR "Postoperative cognitive disorders" OR "Postoperative cognitive dysfunction" OR "Postoperative cognitive complications") AND ("Thoracic Surgery" OR "Cardiac Surgery" OR "Heart Surgery") AND ("Protein expression" OR proteomic OR "Protein profiling"). Eight hundred and twenty-nine studies were retrieved and only clinical studies reporting the circulating preoperative differentially expressed Proteins (DEPs) in the POCD patients were selected. Six studies were selected following the inclusion and exclusion criteria. Only one preoperative DEP and four immediate postoperative DEPs were extracted from the studies. All four proteins were selected for analysis using DAVID, STRING, and Cytoscape software. Due to the very low number of proteins, no clusters have been identified. This systematic review demonstrates the lack of POCD preoperative biomarkers for open-heart-surgery patients. Thus, it is suggested that more studies can be conducted to fill this gap.
    MeSH terms: Humans; Postoperative Complications/blood; Postoperative Complications/etiology; Proteomics/methods; Preoperative Period
  8. Lee JX, Tan YJ, Ismail NAS
    Int J Mol Sci, 2024 Nov 15;25(22).
    PMID: 39596340 DOI: 10.3390/ijms252212275
    NPHS1 and NPHS2 are kidney gene components that encode for nephrin and podocin, respectively. They play a role in the progression of congenital (CNS) and steroid-resistant (SRNS) nephrotic syndrome. Hence, this study aimed to determine the prevalence and renal outcomes of NPHS mutations among pediatric patients with CNS and SRNS. We also aimed to identify potential predictors of NPHS mutations in this patient cohort. Overall, this study included 33 studies involving 2123 patients screened for NPHS1, whereas 2889 patients from 40 studies were screened for NPHS2 mutations. The patients' mean age was 4.9 ± 1 years (ranging from birth to 18 years), and 56% of patients were male (n = 1281). Using the random-effects model, the pooled proportion of NPHS1 mutations among pediatric patients with CNS and SRNS was 0.15 (95% CI 0.09; 0.24, p < 0.001, I2 = 92.0%). The pooled proportion of NPHS2 mutations was slightly lower, at 0.11 (95% CI 0.08; 0.14, p < 0.001, I2 = 73.8%). Among the 18 studies that reported ESRF, the pooled proportion was 0.47 (95% CI 0.34; 0.61, p < 0.001, I2 = 75.4%). Our study showed that the NPHS1 (β = 1.16, p = 0.35) and NPHS2 (β = 5.49, p = 0.08) mutations did not predict ESRF in CNS and SRNS pediatric patients. Nevertheless, patients from the European continent who had the NPHS2 mutation had a significantly higher risk of developing ESRF (p < 0.05, β = 1.3, OR = 7.97, 95% CI 0.30; 2.30) compared to those who had the NPHS1 mutation. We recommend NPHS mutation screening for earlier diagnosis and to avoid unnecessary steroid treatments. More data are needed to better understand the impact of NPHS mutations among pediatric patients with CNS and SRNS.
    MeSH terms: Adolescent; Child; Child, Preschool; Female; Humans; Infant; Infant, Newborn; Male; Mutation*
  9. Abd Rashed A, Jamilan MA, Abdul Rahman S, Amin Nordin FD, Mohd Nawi MN
    Antibiotics (Basel), 2024 Nov 12;13(11).
    PMID: 39596767 DOI: 10.3390/antibiotics13111074
    BACKGROUND/OBJECTIVES: Microorganisms such as bacteria, viruses, and fungi are frequently the cause of infections. Antimicrobial agents, such as antibiotics, antivirals, and antifungals, are used to target and eliminate these infectious agents. On the other hand, inflammation is a natural response of the immune system to injury, infection, or irritation. Although herbal remedies have been used to treat these conditions for centuries and can be effective in certain situations, it is crucial to use them with caution. Not all herbal remedies are supported by scientific evidence, and their safety and efficacy can vary. Thus, we conducted this review to determine the potential health benefits of agarwood as an antimicrobial and anti-inflammatory agent.

    METHODS: Three databases (PubMed, Scopus, and Google Scholar) were used to search for original papers submitted between 2013 and 2023, using the Medical Subject Heading (MeSH) terms "agar-wood" crossed with the terms "antimicrobial" and/or "anti-inflammatory". Synonyms and relevant search terms were also searched.

    RESULTS: The most-studied agarwood for antimicrobial and anti-inflammatory agents is Aquilaria sinensis. Some studies have shown its potential application as a potent inhibitor of fungi, including Lasiodiplodia theobromae, Fusarium oxysporum, and Candida albicans. Moreover, it is capable of inhibiting Bacillus subtilis and Staphylococcus aureus activities. Several chromones detected in agarwood have been shown to inhibit NF-κB activation, LPS-induced NO production, and superoxide anion generation. In conclusion, more research is needed, particularly regarding future intervention studies, to enhance our knowledge and understanding of agarwood and its isolates.

    CONCLUSIONS: This review reveals that despite the absence of clinical trials, agarwood exhibits antimicrobial and anti-inflammatory properties.

  10. Basir A, Sulong AB, Muhamad N, Juri AZ, Jamadon NH, Foudzi FM, et al.
    Materials (Basel), 2024 Nov 13;17(22).
    PMID: 39597359 DOI: 10.3390/ma17225536
    Two-component micro-powder injection moulding (2C-μPIM) is a prospective approach for fabricating bi-material micro-components of stainless steel 316L (SS316L) and 3 mol% yttria-stabilised zirconia (3YSZ) at an appealing cost. However, the fundamental challenge lies in preventing the formation of large-scale cracks at the interface of two different materials during sintering. This study investigated how SS316L nanoparticles in bimodally configured SS316L powder that incorporated both nanoparticles and microparticles influenced the sintering of 2C-μPIM-processed miniature bi-materials made of bimodal SS316L and 3YSZ. In this study, feedstocks were developed by integrating monomodal (micro-sized) SS316L powder, three types of nano/micro-bimodal SS316L powders, and 3YSZ powder individually with palm stearin and low-density polyethylene binders. The results indicated that increasing the SS316L nanoparticle content to 45 vol.% caused a 19.5% increase in the critical powder loading in the bimodal SS316L powder as compared to that in the monomodal SS316L powder. The addition of SS316L nanoparticles increased the relative density and hardness of the sintered bi-materials, with the maximum values obtained being 96.8% and 1156.8 HV, respectively. Field emission scanning electron microscopy investigations revealed that adding 15 vol.% and 30 vol.% SS316L nanoparticle contents reduced interface cracks in bi-materials significantly, while 45 vol.% resulted in a crack-free interface.
  11. Low ZX, Kanauchi O, Tiong V, Sahimin N, Lani R, Tsuji R, et al.
    Microorganisms, 2024 Nov 13;12(11).
    PMID: 39597693 DOI: 10.3390/microorganisms12112304
    The growing risk of contracting viral infections due to high-density populations and ecological disruptions, such as climate change and increased population mobility, has highlighted the necessity for effective antiviral treatment and preventive measures against Dengue virus (DENV), Chikungunya virus (CHIKV), and Zika virus (ZIKV). Recently, there has been increasing attention on the use of probiotics as a potential antiviral option to reduce virus infections. The present study aimed to assess the immunomodulatory effects of heat-killed Lactococcus lactis strain plasma (LC-Plasma) on peripheral blood mononuclear cells (PBMCs) and its subsequent antiviral response against DENV, CHIKV, and ZIKV. To evaluate the immunomodulatory effects of LC-Plasma on PBMCs isolated from healthy individuals, PBMCs were cultured at a density of 2 × 105 cells/well and stimulated with 10 µg/mL of LC-Plasma. LC-plasma-stimulated PBMCs demonstrated elevated interferon-alpha (IFN-α) production and cluster of differentiation 86 (CD86) and human leukocyte antigen-DR isotype (HLA-DR) upregulation, potentially linked to plasmacytoid dendritic cell (pDC) activation. The replication of DENV, CHIKV, and ZIKV was dose-dependently inhibited when Huh-7 cells were stimulated with LC-Plasma-stimulated PBMC supernatant (LCP Sup). IFN-stimulated gene (ISG) expression, including IFN-stimulated gene 15 (ISG15), IFN-stimulated exonuclease gene 20 (ISG20), IFN-induced transmembrane protein 1 (IFITM-1), myxovirus resistance protein A (MxA), and radical S-adenosyl methionine domain-containing protein 2 (RSAD2), was significantly upregulated in LCP Sup-stimulated Huh-7 cells. Findings from this study indicate that LC-Plasma has the potential to induce IFN-α production, leading to an enhancement in the expression of ISGs and contributing to a broad-spectrum antiviral response. Thus, LC-Plasma may serve as a rational adjunctive treatment to ameliorate viral diseases, warranting future clinical trials.
  12. Fadzil MAM, Abu Seman N, Abd Rashed A
    Pharmaceuticals (Basel), 2024 Nov 18;17(11).
    PMID: 39598457 DOI: 10.3390/ph17111548
    INTRODUCTION: In 2019, 9.3% (463 million) of adults worldwide had diabetes, according to the International Diabetes Federation (IDF). By 2030, the number will rise to 10.2% (578 million) and 10.9% (700 million) by 2045 if effective prevention methods are not implemented. Agarwood is a pathological product and valuable plant due to its numerous medicinal properties, and it is used as an essential ingredient in medicine. Therefore, we conducted this review to determine agarwood's potential health benefit effect on type 2 diabetes.

    RESULTS AND DISCUSSION: Although no clinical trials were found, the evidence from in vitro and in vivo studies is promising. Agarwood has shown the ability to reduce the activity of α-glucosidase, α-amylase, and lipase, promote adiponectin secretion during adipogenesis, and reduce oxidative stress. Animal studies elucidated hypoglycaemic, antidyslipidemia, anti-obesity, and organ protective effects from agarwood.

    MATERIALS AND METHODS: Original articles were searched in three databases (PubMed, Scopus, and Cochrane Library) using the medical subject heading (MeSH) term "diabetes" crossed with the term "agarwood" from 2008 to 2024. Synonyms and relevant search terms were also searched.

    CONCLUSIONS: This effect underscores the need for further research and the potential for groundbreaking discoveries in the field of diabetes treatment.

  13. Tyubaeva PM, Varyan IA, Romanov RR, Merzlikin VA, Gruznova OA, Gruznov DV, et al.
    Polymers (Basel), 2024 Nov 20;16(22).
    PMID: 39599313 DOI: 10.3390/polym16223221
    This work is devoted to the creation of biocompatible fibrous materials with a high antimicrobial effect based on poly-3-hydroxybutyrate (PHB) and chlorophyll (Chl). The data obtained show the possibility of obtaining fibrous materials from PHB and Chl by electrospinning methods. The obtained electrospun matrices were investigated by the SEM, DSC and FTIR methods. Various key properties of the matrices were evaluated, including hydrophilicity and mechanical strength, as well as photodynamic and light-dependent antimicrobial effects against the conditionally pathogenic microorganism Staphylococcus aureus. The results demonstrate a significant improvement in electrospinning properties for a concentration of 0.5% Chl and a reduction in fiber formation defects, as well as an increase in the strength of nonwovens. It was found that the antimicrobial potential of Chl-PHB (with concentrations of Chl of 1.25 and 1.5%) is higher than that of Chl in free form. It was also determined that irradiation increases the inhibitory effect of Chl, both in free form and in the form of a complex with a polymer.
  14. Almassri HF, Abdul Kadir A, Srour M, Foo LH
    Nutrients, 2024 Nov 20;16(22).
    PMID: 39599746 DOI: 10.3390/nu16223960
    BACKGROUND: This study emphasizes the critical role of early nutritional interventions in addressing cancer-related malnutrition. It aimed to assess the effects of omega-3 fatty acids (ω3) and vitamin D3 (VitD) supplementation on the nutritional status of newly diagnosed women with breast cancer (BC) in the Gaza Strip, Palestine.

    METHOD: A total of 88 newly diagnosed women with BC were randomly assigned into four groups: (i) Omega-3 fatty acid (ω3) group; (ii) Vitamin D (VitD) group; (iii) ω3+VitD group; and (iv) the controls. The patients took two daily 300 mg ω3 capsules and/or one weekly 50,000 IU VitD tablet for nine weeks. Nutritional status of the participants was assessed by several measurement tools, namely, the Patient-Generated Subjective Global Assessment (PG-SGA)-derived scores, anthropometric measurements, blood albumin status and dietary intakes between the baseline and after 9 weeks post-intervention. The procedures of the present study were registered on ClinicalTrial.gov with the identifier NCT05331807.

    RESULTS: At the end of trial, there was a significant increase in the PG-SGA-derived nutritional risk scores (p < 0.01), body weight and body mass index (BMI) (both p < 0.05) among participants in ω3+VitD group compared to other groups. Additionally, there was a significant rise in blood albumin levels (p < 0.05), daily energy and protein intake in the ω3+VitD group (p < 0.05) compared to baseline.

    CONCLUSION: Participants with supplementation of daily ω3 and weekly VitD had improved nutritional status, assessed by the PG-SGA scores and anthropometric measures, blood albumin and dietary energy and protein intake among women with BC who were undergoing active treatment.

    MeSH terms: Adult; Breast Neoplasms*; Female; Humans; Middle Aged; Middle East; Nutritional Status*; Vitamin D/administration & dosage; Vitamin D/blood; Arabs; Dietary Supplements*
  15. El-Sayed SE, Abdelaziz NA, Alshahrani MY, El-Housseiny GS, Aboshanab KM
    Future Sci OA, 2024 Dec;10(1):2430452.
    PMID: 39600180 DOI: 10.1080/20565623.2024.2430452
    Alcaligenes faecalis is a Gram-negative, rod-shaped bacterium that is common in the environment and has been reported to have various bioactive metabolites of industrial potential applications, including antifungal, antibacterial, antimycobacterial, antiparasitic, anticancer, antioxidant activities. In this review, we highlighted and discussed the respective metabolites, pointing out their chemical diversities, purification, current challenges, and future directions. A. faecalis has an industrial role in biodegradation, biosurfactants, and different enzyme production. In this review, the up-to-date various Response Surface Methodology methods (RSM) that can be employed for statistical optimization of the bioactive secondary metabolites have been discussed and highlighted, pointing out the optimal use of each method, current challenges, and future directions.
  16. Ahmad NA, Mat Ludin AF, Vanoh D, Tohit NM, Manaf ZA, Mohd Noah SA, et al.
    Digit Health, 2024;10:20552076241297213.
    PMID: 39600385 DOI: 10.1177/20552076241297213
    BACKGROUND: Technology advancement along with the increase in the older adults' population leads to the creation of health applications. The combination of exercise, nutrition, and cognition should be studied carefully in improving older adults' health.

    OBJECTIVE: The purpose of this study is to develop a health application, WeFit contains these three components and to determine its content validity, acceptability, and usability.

    METHODOLOGY: This study is a design and development study involving three phases. The first phase is the need analysis involving a review on 16 mobile applications available in Google play and iTunes App store as well as a review of six articles for identifying the perception of older adults in using mobile applications. Second phase is mobile application development and content validity. The content validity was determined using the Content Validity Index for Individual Items (I-CVI). Phase 3 evaluated the acceptance of the WeFit mobile application among older adults and health practitioners.

    RESULTS: Phase 1 indicated that half of the applications reviewed (50.0%) had physical activity component and the other half (50.0%) had a cognitive component, and none on nutrition. No application is reported to have all three components. In Phase 2, WeFit health application containing the three components was developed where users can view exercise and food recommendations and play cognitive games. WeFit had an I-CVI value of 0.98. With respect to acceptability, majority of the study participants (93.3%) understood the WeFit's content and the graphics used were appropriate. The usability study found that the majority of the older adults were satisfied with the interface and content. All health practitioners (100%) agreed WeFit is easy to use and agreed that it can guide them in giving medical advices.

    CONCLUSION: WeFit mobile app has been successfully developed, validated, and tested for acceptance among the older adults and health practitioners.

  17. Feng H, Qin Y, Li Y, Li S, Zheng Y, Yan J, et al.
    Clin Cosmet Investig Dermatol, 2024;17:2595-2613.
    PMID: 39600528 DOI: 10.2147/CCID.S479632
    AIM: The application of bibliometric analysis gives insights into research trend and future perspectives. This paper aims to understand the current status of studies on traditional Chinese medicine (TCM) in the treatment of atopic dermatitis (AD), and to investigate the cooperation network, hotspots and trends in the field.

    METHODS: The original data were retrieved from the Web of Science Core Collection by searching for the topic "atopic dermatitis" and "traditional Chinese medicine" with the time span of 2004-2023. Microsoft Excel was used to create statistical tables and charts, whereas CiteSpace and VOSViewer were applied for knowledge mapping.

    RESULTS: In total, 252 publications were identified and downloaded. Current research on TCM treating AD is in the steady growth period. China has the largest number of publications, whereas the USA has the greatest international influence. Notably, the Arab Emirates, Malaysia, India and Bangladesh all have a high research impact but with a low amount of publications. Universities in Hong Kong China have the largest number of publications, but their interaction with other institutions is very poor. The top three most productive authors are all from Hong Kong, having a strong collaboration network with each other. The popular research topics in this field are "children" "cell" "activation" "nf kappa b" "inflammation" "asthma" "extract" "management" and "cytokine". According to the analysis of leading co-cited journals and authors, "J ETHNOPHARMACOL" has the largest amount of citations whilst "AM J CHINESE MED" the strongest academic influence. The largest number of co-cited authors comes from "Hon Karn-Lun Ellis", a well-known scholar from Hong Kong.

    CONCLUSION: TCM has been widely used in AD treatment, honored for its individualized therapeutic concepts of evidence-based treatment. The academic community must form a continuous and deep-going pattern to achieve higher international influence and a stronger research degree in this field.

  18. Tan HJ, Tan EZ
    World J Gastrointest Endosc, 2024 Nov 16;16(11):623-626.
    PMID: 39600554 DOI: 10.4253/wjge.v16.i11.623
    Atrophic gastritis and intestinal metaplasia may progress to gastric malignancy. Non-invasive serum biomarkers have been extensively studied and proven to be useful as a screening tool to stratify risk and identify patients for endoscopy to detect early gastric cancer. These non-invasive biomarkers have been endorsed and recommended by many international consensus guidelines. In this letter, we reviewed the literature and evidence supporting the use of serum biomarkers as a dynamic test to monitor the status of atrophic gastritis.
  19. Mohd Ishar NI, Mior Harun MH, Hanif A, Mustapha NA, Kusa R, Duda J
    Open Res Eur, 2024;4:191.
    PMID: 39600592 DOI: 10.12688/openreseurope.18248.1
    An unprecedented surge in digital payments has had a profound effect on the economic development of developing nations. This transition has enabled greater financial inclusion through the provision of banking services to populations that were previously marginalised. In addition, it has reduced reliance on physical currency, thereby improving security and transparency. This bibliometric analysis examines the research on digital payments in Malaysia and Poland, providing a comparative perspective on research patterns and contributions in these two countries. Furthermore, this study offers a comprehensive examination of the research environment in Malaysia and Poland, elucidating the distinct approaches, methodology, and areas of emphasis in each nation. These findings provide useful insights for those interested in championing digital payment initiatives, as well as contributing to a more robust and resilient digital payment framework. As digital payment ecosystems mature, their significance in altering the economic landscape of emerging countries is likely to become more evident in facilitating cross-border knowledge exchange and collaboration.
  20. Aziz A, Ganesan Nathan K, Kamarul T, Mobasheri A, Sharifi A
    Ther Adv Musculoskelet Dis, 2024;16:1759720X241299535.
    PMID: 39600593 DOI: 10.1177/1759720X241299535
    BACKGROUND: Osteoarthritis (OA) is a common degenerative joint disease that poses a significant global healthcare challenge due to its complexity and limited treatment options. Advances in metabolomics have provided insights into OA by identifying dysregulated metabolites and their connection to altered signaling pathways. However, a comprehensive understanding of these biomarkers in OA is still required.

    OBJECTIVES: This systematic review aims to identify metabolomics biomarkers associated with dysregulated signaling pathways in OA, using data from various biological samples, including in vitro models, animal studies, and human research.

    DESIGN: A systematic review was conducted following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

    DATA SOURCES AND METHODS: Data were gathered from literature published between August 2017 and May 2024, using databases such as "PubMed," "Scopus," "Web of Science," and "Google Scholar." Studies were selected based on keywords like "metabolomics," "osteoarthritis," "amino acids," "molecular markers," "biomarkers," "diagnostic markers," "inflammatory cytokines," "molecular signaling," and "signal transduction." The review focused on identifying key metabolites and their roles in OA-related pathways. Limitations include the potential exclusion of studies due to keyword selection and strict inclusion criteria.

    RESULTS: The meta-analysis identified dysregulated metabolites and associated pathways, highlighting a distinct set of related metabolites consistently altered across the studies analyzed. The dysregulated metabolites, including amino acids, lipids, and carbohydrates, were found to play critical roles in inflammation, oxidative stress, and energy metabolism in OA. Metabolites such as alanine, lysine, and proline were frequently linked to pathways involved in inflammation, cartilage degradation, and apoptosis. Key pathways, including nuclear factor kappa B, mitogen-activated protein kinase, Wnt/β-catenin, and mammalian target of rapamycin, were associated with changes in metabolite levels, particularly in proinflammatory lipids and energy-related compounds.

    CONCLUSION: This review reveals a complex interplay between dysregulated metabolites and signaling pathways in OA, offering potential biomarkers and therapeutic targets. Further research is needed to explore the molecular mechanisms driving these changes and their implications for OA treatment.

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