Browse publications by year: 2025

  1. Ding CH, Wahab AA, Mohamed N, Wong PF
    Trop Biomed, 2025 Mar 01;42(1):58-60.
    PMID: 40163405 DOI: 10.47665/tb.42.1.010
    Bacteremia due to Granulicatella adiacens has been rarely reported in the medical literature. A middleaged gentleman developed necrotizing fasciitis on his left second toe after stepping on a nail. A ray amputation was performed and ceftazidime-susceptible Pseudomonas aeruginosa was isolated from his bone culture. However, while receiving ceftazidime for the necrotizing fasciitis, his blood culture vial was positive for gram-positive cocci-shaped bacteria in short chains which grew as tiny non-lytic colonies on sheep blood agar only following extended incubation. There was no culture evidence of P. aeruginosa in the same blood specimen. The gram-positive organism was conclusively identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry as G. adiacens. The patient was treated with benzylpenicillin (to which the organism tested susceptible) for 14 days before he was discharged home.
    MeSH terms: Humans; Male; Middle Aged; Penicillin G/therapeutic use; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Fasciitis, Necrotizing/diagnosis; Fasciitis, Necrotizing/microbiology
  2. Du ST, Yang YW, Wu TF
    Trop Biomed, 2025 Mar 01;42(1):61-64.
    PMID: 40163406 DOI: 10.47665/tb.42.1.011
    To investigate the seroprevalence of Toxoplasma gondii infection in patients with malignant tumors, we aimed to provide insights into improving the prognosis and quality of life of these patients. A total of 721 patients with malignant tumors admitted to the Affiliated Hospital of Dali University from November 2020 to May 2022, along with 100 healthy volunteers from the hospital during the same period, were enrolled. Demographic data and serum samples were collected, and serum-specific IgG and IgM antibodies against T. gondii were detected using an enzyme-linked immunosorbent assay. Patients were considered positive for T. gondii infection if either IgG or IgM antibodies were detected. The seroprevalence of specific IgG and IgM antibodies was compared between patients with malignant tumors and healthy volunteers. The positivity rates for anti-T. gondii antibodies in patients with malignant tumors was significantly higher than that in healthy volunteers (31.2% vs. 13.0%; χ2 = 14.141, P < 0.01). Pairwise comparisons revealed that the positive rates of serum anti-T. gondii antibodies in patients with various types of malignant tumors were significantly higher than those in healthy volunteers (all P values < 0.01). There was no statistically significant difference in the average age and sex composition between patients with malignant tumors and healthy volunteers (P > 0.05). The results indicate a high seroprevalence rate of T. gondii infections among patients with malignant tumors, which was significantly higher than that among healthy volunteers.
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; China/epidemiology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Middle Aged; Seroepidemiologic Studies; Young Adult
  3. Pechdee P, Arunsan P, Boonsuya A, Thanchonnang C, Phinsiri S, Rattanapitoon NK, et al.
    Trop Biomed, 2025 Mar 01;42(1):65-75.
    PMID: 40163407 DOI: 10.47665/tb.42.1.012
    The human liver fluke, Opisthorchis viverrini, is a significant risk factor for cholangiocarcinoma (CCA) in Thailand. Praziquantel (PZQ) is the primary treatment for opisthorchiasis but is associated with adverse effects and the potential development of CCA amid chronic infection and reinfection, prompting the search for alternative treatments. Thunbergia laurifolia is widely used for detoxification from lead poisoning and other toxins. The bioactive compounds of T. laurifolia are categorized into sterols, phenolics, carotenoids, glycosides, and unclassified steroids. It is considered an herb that contains a variety of active compounds, which may demonstrate effectiveness in eliminating parasites. This study evaluated the anthelmintic efficacy of crude extracts of Thunbergia laurifolia on newly excysted juveniles (NEJs) and adult stages of O. viverrini.The experiments were involved treating O. viverrini NEJs and adult worms with various concentrations of the crude extracts (NEJs: 5, 10, 20, 40 mg/ml; adult worms: 20, 30, 40 mg/ml), with PZQ and RPMI-1640 medium serving as positive and negative controls, respectively. An assessment of relative motility (RM) and survival index (SI) was conducted, along with a morphological evaluation using scanning electron microscopy (SEM) and biochemical tests to evaluate the generation of reactive oxygen species (ROS) as a response to stress. Results showed that T. laurifolia crude extract reduced RM and SI of the O. viverrini NEJs and adult worms. SEM revealed minimal tegumental damage when compared to the positive control group. Elevated ROS levels were significantly higher, suggesting oxidative stress as a potential mechanism of action. These findings suggest that T. laurifolia possess promising anthelmintic properties against O. viverrini, meriting further research to isolate active compounds and elucidate their mechanisms. Such studies could lead to the development of novel herbal treatments for opisthorchiasis.
    MeSH terms: Animals; Thailand; Complex Mixtures/pharmacology
  4. Lv Y, Wang C, Bai L
    Trop Biomed, 2025 Mar 01;42(1):76-84.
    PMID: 40163408 DOI: 10.47665/tb.42.1.013
    Tuberculosis (TB), an infectious disease caused by the bacterium Mycobacterium tuberculosis (Mtb), is a major cause of morbidity and mortality worldwide. Annually, millions of new cases of tuberculosis are documented. Research in tuberculosis-immune has hitherto focused predominantly on the role of T cells in Mtb infection. Although there have been studies in progress which have supported the notion that B cells are crucial players in combating infectious diseases, the role of B cells in TB is still not fully understood. There is a paucity of in-depth analysis of various B lymphocyte subpopulations and the understanding of the immunophenotypic changes of the B-cell lineage during tuberculosis infection. Therefore, we aimed to investigate the changes in B lymphocyte subpopulations in mice infected with M.tuberculosis H37Ra. The percentage/frequency of peritoneal B cells (CD45R+), B1b cells (CD45RIntIgDCD5- CD11+), splenic B cells (CD45R+), and splenic marginal zone B (MZ B) cells (CD45R+CD23- CD21hi) decreased (P < 0.05), while the percentage of splenic follicular B (Fo B) cells (CD45R+CD23+ CD21int) and lymph node B cells (CD19+) increased at the 4th and 8th weeks (P < 0.05). It was suggested that H37Ra infection changed the distribution of B lymphocyte subpopulations. In addition, the percentage of CD69+B cells and memory B cells (CD45R+CD27+ IgD+/-) increased in the infected mice at different infection periods (P < 0.05), which suggested H37Ra infection promoted B cell activation and produced a large number of memory B cells. As a conclusion, H37Ra infection can affect the distribution of B lymphocyte subpopulations, with a concomitant down-regulation of MZ B cells, which perform innate immunity, and up-regulation of adaptive immune response cells (Fo B cells and lymph node B cells). Furthermore, it has been demonstrated that H37Ra infection can promote the immune response of B lymphocytes to tuberculosis, through the stimulation of the body to produce a large number of activated and memory B cells.
    MeSH terms: Animals; B-Lymphocytes/immunology; Female; Lymph Nodes/immunology; Mice, Inbred BALB C; Mice
  5. Goh SL, Yap KS, Neo ERK, Koo CW, Madhavan U, Suwandi NA, et al.
    Waste Manag, 2025 Mar 30;200:114760.
    PMID: 40163956 DOI: 10.1016/j.wasman.2025.114760
    Plastic waste has a significant environmental impact when mismanaged. The study quantifies the environmental impact of plastic waste management systems using consequential life cycle assessments in selected countries in Asia, including India, Indonesia, Malaysia, the Philippines, Thailand, and Vietnam. The scope covers from plastic waste generation to end-of-life treatments, including environmental impact avoided from not producing raw materials due to plastic waste utilization. Eleven environmental impacts were considered, including global warming potential (GWP100) and carcinogenic human toxicity (HTPc). India has the lowest net GWP100 (-0.648 kg CO2-eq) per kg of plastic waste while Indonesia has the highest (1.128 kg CO2-eq). Future scenario analyses showed that increasing recycling can reduce the GWP100 of plastic waste management systems by up to 611 % by 2030. Policymakers and investors can use these results to focus their efforts on waste management methods which reduce the impact of plastic waste on the environment and human health.
  6. Tan YH, Poong SW, Beardall J, Phang SM, Lim PE
    Mar Environ Res, 2025 Mar 21;208:107095.
    PMID: 40163968 DOI: 10.1016/j.marenvres.2025.107095
    Phytoplankton are cosmopolitan marine photosynthetic organisms that are vital to biogeochemical cycles and marine ecosystems. The current rise in atmospheric CO2 and surface ocean temperatures are poised to disrupt the ecological niches of phytoplankton. Picochlorophytes, a broad taxon of small green eukaryotic phytoplankton, have been shown to perform well under future rising oceanic CO2 and temperature scenarios. This study investigates the acclimation responses of cosmopolitan picochlorophytes from the Chlorella-lineage under high CO2 (1000 p.p.m.) and a rise of 4 °C (8 °C - polar picochlorophyte; 32 °C, tropical picochlorophyte). In order to determine how the future ocean warming and acidification might affect picochlorophytes, a polar strain of Chlorella and a tropical Parachlorella were selected, and their physiology and GCMS-based metabolomics were investigated. Growth rate and cellular dimensions (diameter, volume, and surface area) of Chlorella significantly increased in all environmental future scenarios compared to Parachlorella. Photosynthetic parameters of the picochlorophytes studied showed acclimation, with high temperature and high CO2 triggering the adaptation of Fv/Fm, NPQmax, and Ek of Chlorella and Parachlorella, respectively. High CO2 induced the most changes in the Chlorella metabolome, altering the levels of metabolites related to amino acids and their derivatives, glutathione production, carbohydrates, and photochemical quenching. Combined high CO2/temperature altered Parachlorella's metabolome, though with a small number of biomarkers detected. This study provided evidence to support the hypothesis that picochlorophytes could thrive in a more acidified and warmer ocean.
  7. Al Jaberi FM, Alzarzour R, Dewa A, Muhamad A, Zakaria F
    Behav Brain Res, 2025 Mar 29;486:115559.
    PMID: 40164316 DOI: 10.1016/j.bbr.2025.115559
    Obesity is a growing public health concern that significantly impacts cognitive functions, including memory. This research explores how a high-fat diet affects short-term memory, employing the novel object recognition (NOR) test and NMR-based metabolomics to elucidate metabolic alterations in the brain and adipose tissue. The zebrafish were divided into two groups: one receiving a standard diet (SD) and the other a high-fat diet (HFD). Body mass index (BMI) was assessed every two weeks for a period of eight weeks. The NOR test was used to determine the discrimination index (DI) for evaluating the short-term memory of the SD and HFD groups. NMR spectroscopy was employed to investigate the metabolites in brain and adipose tissues, and multivariate data analysis was conducted to discover significant metabolic alterations. The high-fat diet (HFD) resulted in a significant increase in body mass index (BMI) (p 
  8. Zhou X, Chen S, Zhou C, Jin Z, He H, Bai Y, et al.
    Int J Oral Sci, 2025 Apr 01;17(1):20.
    PMID: 40164594 DOI: 10.1038/s41368-025-00357-9
    The prevalence of Class III malocclusion varies among different countries and regions. The populations from Southeast Asian countries (Chinese and Malaysian) showed the highest prevalence rate of 15.8%, which can seriously affect oral function, facial appearance, and mental health. As anterior crossbite tends to worsen with growth, early orthodontic treatment can harness growth potential to normalize maxillofacial development or reduce skeletal malformation severity, thereby reducing the difficulty and shortening the treatment cycle of later-stage treatment. This is beneficial for the physical and mental growth of children. Therefore, early orthodontic treatment for Class III malocclusion is particularly important. Determining the optimal timing for early orthodontic treatment requires a comprehensive assessment of clinical manifestations, dental age, and skeletal age, and can lead to better results with less effort. Currently, standardized treatment guidelines for early orthodontic treatment of Class III malocclusion are lacking. This review provides a comprehensive summary of the etiology, clinical manifestations, classification, and early orthodontic techniques for Class III malocclusion, along with systematic discussions on selecting early treatment plans. The purpose of this expert consensus is to standardize clinical practices and improve the treatment outcomes of Class III malocclusion through early orthodontic treatment.
    MeSH terms: Child; Humans; Orthodontics, Corrective; Consensus
  9. Siraji MA, Karim F, Soto CJ, Haque S
    Sci Rep, 2025 Mar 31;15(1):11008.
    PMID: 40164706 DOI: 10.1038/s41598-025-90264-0
    The Big Five Inventory-2 (BFI-2) is a widely recognized tool for assessing personality traits across five domains and fifteen facets. However, its psychometric properties in non-Western cultures like Bangladesh remain unexplored. This study aimed to validate the Bangla BFI-2 (BFI-2-B) within a Bangladeshi community sample to provide a culturally adapted personality assessment tool. A cross-sectional survey was conducted on 1,095 participants, where 646 participants (59%; 358 female; Agemean = 24.25 years, SD = 4.47) passed all attention checks. Participants responded to a demographic questionnaire, Bangla Big Five-2 (BFI-2-B), and Bangla NEO Five-Factor Inventory (NEO FFI). The domain-level structural validity was analyzed using Exploratory Structural Equation Modeling (ESEM). A series of five different latent models were tested by Confirmatory Factor Analysis (CFA) for facet-level structural validity. Full Measurement invariance across gender, education level, and language were tested. The item quality was assessed using Item Response Theory analysis (IRT). Convergent and discriminant validity were assessed by correlating the BFI-2-B domains with the NEO-FFI. The BFI-2-B demonstrated high internal consistency across domains (> 0.70) and facets (> 0.60; except energy level, intellectual curiosity, and respectfulness). ESEM confirmed structural validity at the domain level (CFI & TLI = 0.96). CFA analysis revealed that at the facet level, a three-facet structure with an acquiescence factor yielded the most acceptable fit (CFI& TLI ≥ 0.95; RMSEA ≤ 0.06; SRMR ≤ 0.08). Full measurement invariance was established across gender and educational levels, but only weak invariance was found across languages, indicating linguistic challenges. The similar domains of BFI-2-B and NEO-FFI had strong correlations (r ≥ 0.59), and distinct domains exhibited low correlations, indicating strong convergent and discriminant validity. IRT analysis showed that most items had high to moderate discrimination. The BFI-2-B is a reliable and valid tool for assessing personality in the Bangladeshi context, with robust psychometric properties across domains and most facets. Addressing linguistic nuances and testing in more diverse samples can further enhance its cross-cultural applicability.The stage 1 protocol for this Registered Report was accepted in principle on 24/09/24. The protocol, as accepted by the journal, can be found at: 10.17605/OSF.IO/7DTQG.
    MeSH terms: Adolescent; Adult; Bangladesh; Cross-Sectional Studies; Factor Analysis, Statistical; Female; Humans; Language; Male; Middle Aged; Personality; Personality Assessment; Personality Inventory/standards; Surveys and Questionnaires; Reproducibility of Results; Young Adult
  10. Qin F, Zain AM, Zhou KQ, Zhuo DB
    Sci Rep, 2025 Mar 31;15(1):11012.
    PMID: 40164738 DOI: 10.1038/s41598-025-95406-y
    Weighted Fuzzy Production Rules (WFPRs) are vital for Clinical Decision Support Systems (CDSSs), significantly impacting diagnostic accuracy and bridging the gap between data-driven insights and actionable clinical decisions through knowledge engineering. This paper proposes an integrated approach combining the Dynamic Dimension Adjustment Harmony Search (DDA-HS) Algorithm and Back Propagation Neural Networks (BPNNs) to enhance WFPR extraction accuracy. DDA-HS dynamically adjusts search space dimensions through fitness evaluations, optimizing initial weights in BPNNs and leveraging an absorbing Markov chain to enhance transition probabilities, supporting exploration and avoiding local optima in high-dimensional spaces. Evaluated against existing optimization methods including Harmony Search (HS), Cuckoo Search (CS), Adaptive Global Optimal Harmony Search (AGOHS), and Harmony Search with Cuckoo Search (HSCS) Algorithms, DDA-HS achieves 74.48% accuracy for BPNN classification and 77.08% for WFPR classification on the PIMA dataset, representing improvements of 3.6% and 6.5%, respectively. WFPR extraction enhances BPNN interpretability by revealing feature influences on decision-making, improving both accuracy and transparency. The proposed method offers a robust framework for reliable and interpretable CDSSs in healthcare.
  11. Alias A, Ramli M, Deventhiran KV, Siddique MNI, Yahaya N, Heděnec P
    PMID: 40164890 DOI: 10.1007/s12223-025-01257-5
    Vermicomposting has been recognized as a sustainable solution for the managing of organic waste, primarily because of the bacterial communities that drive microbial decomposition. However, while the roles of abundant bacteria in composting processes are well-documented, the contributions of rare bacteria remain underexplored. In this study, we investigated the diversity and composition of abundant and rare bacterial communities in gut of Eudrilus eugeniae and resulting vermicompost, comparing okara as a single substrate compared to a combination of more substrates, such as kitchen waste or okara and kitchen waste. Amplicon sequencing revealed a total of 3085 operational taxonomic units (OTUs), comprising 188 abundant OTUs and 2127 rare OTUs. Significant differences in bacterial community composition were observed between vermicompost and the earthworm gut, particularly in the rare bacterial communities, with distinct abundances of Gemmatimonadota, Desulfobacteria, Myxococcota, Acidobacteria, and Firmicutes. Interestingly, no significant differences were found between treatments in the abundant bacterial communities, suggesting that okara alone can sustain a bacterial community comparable to mixed substrates. However, rare bacterial communities were more sensitive to substrate variation, with okara fostering distinct rare microbial populations in the vermicompost and earthworm gut. Our results indicate okara can support both abundant and rare bacteria, producing compost with similar physico-chemical properties to those derived from mixed substrates. The study highlights the importance of rare bacterial communities in vermicomposting and demonstrates the potential of okara as a valuable resource for sustainable waste management and soil improvement.
  12. Adamu S, Ali MB, Desa MNM, Neoh HM, Masri SN, Joseph N, et al.
    BMC Genomics, 2025 Mar 31;26(1):322.
    PMID: 40165075 DOI: 10.1186/s12864-025-11215-7
    The resistance of ESBLs-producing Kp to various groups of antibiotics commonly used against infections they caused had become a global threat and required urgent attention. This study assessed the extended spectrum beta-lactamases (ESBLs)-producing Klebsiella pneumoniae isolates in terms of their genomic resistance. An analytical profile index (API) 20E kit was used to confirm a total of 100 clinical isolates of ESBL Klebsiella pneumoniae. The disc diffusion method was used to perform the antimicrobial susceptibility testing (AST), which was followed by the phenotypic detection of ESBLs. Six profiled representative ESBL positive strains were subjected to whole genome sequencing (WGS), multilocus sequence typing (MLST), and phylogenetic tree construction using the sequence data. The study showed that 46(46%) of the 100 isolates were positive for ESBL production and antibiotic susceptibility testing revealed significant resistance to β-lactam antibiotics including monobactam especially ampicillin/sulbactam (40%), cephalosporin groups (cefuroxime, cefotaxime, and ceftriaxone) stood at 51%, 49% and 48% respectively and aztreonam with 49%. The WGS analysis of the representative strains revealed genes encoding resistance to aminoglycoside (StrA4, StrB1, aac(3')-IIa, aac(6')-1b, aac(6')1b-cr-1, aadA16, aph(3')-VIa and aadA15), trimethoprim (dfrA14 and dfrA27), sulphonamide (sul1_11, sul2_2 and sul2_3), quinolone (QnrB40-1, QnrB10, QnrS2, OqxA and OqxB), tetracycline (tet(A)_4), fosfomycin (fosA3, floR2 and fosA7), macrolid (mph(A)_1), rifampicin (ARR-3), β-lactam (blaCTX-M-15_23, blaCTX-M-55, blaSHV-1_22, blaSHV11_18, blaSHV-11, blaSHV-1_1.1, blaSHV-11_3, blaSHV-11_19, blaTEM-1_1, blaTEM-1_5, blaOXA-51_10, blaOXA-30_1, blaNDM-1, blaLEN6, blaLEN8 and blaLEN21 were detected. The MLST analysis revealed two novel sequence types of representative strains (2 with ST NF* and 12 with ST NF) and four other heterogeneous STs which include ST394, ST985, ST17 and ST11 while the phylogenetic tree of the strains showed closed clonal relationship and lineages with other reference isolates. In conclusion, the study's results showed a high prevalence of ESBL-producing Kp in the study area, and the representative strains' genomic contents demonstrated that ESBL-producing Kp in a clinical setting could serve as a reservoir for resistance genes and be the source of genetic transfer to other bacterial species. As a result, ongoing surveillance is required to monitor this endemic situation to prevent an epidemiological outbreak of K. pneumoniae- carrying ESBL.
    MeSH terms: Anti-Bacterial Agents/pharmacology; Hospitals; Humans; Klebsiella Infections/microbiology; Malaysia; Microbial Sensitivity Tests; Phylogeny*; Genome, Bacterial; Multilocus Sequence Typing
  13. Chan YY, Hairi NN, Choo WY, Seman Z, Omar MA, Khamal NR, et al.
    BMC Prim Care, 2025 Mar 31;26(1):92.
    PMID: 40165077 DOI: 10.1186/s12875-025-02793-2
    BACKGROUND: Intimate partner violence (IPV) is the most prevalent form of violence against women globally, leading to various adverse health consequences. Primary healthcare providers (PHCPs) are often the first point of contact for identifying and managing IPV. However, research on PHCPs' responses to IPV in low- and middle-income countries, including Malaysia, remains limited. This study aimed to determine the perceived preparedness to respond to IPV and its associated factors among PHCPs in Malaysia.

    METHODS: This cross-sectional study involved 1505 PHCPs selected through multistage stratified cluster random sampling from public primary healthcare clinics in Malaysia. Data were collected via self-administered online surveys using the validated Physician Readiness to Manage Intimate Partner Violence Survey (PREMIS)-Malay tool. Complex sample analysis of descriptive data, general linear model (GLM) and logistic regression were performed. The GLM was used to determine knowledge and opinion score variables predicting the mean perceived preparedness score, whereas multivariable logistic regression identified factors associated with a good level of perceived preparedness to manage IPV. A p-value ≤ 0.05 was considered statistically significant.

    RESULTS: Most Malaysian PHCPs (81.0%) had not received any IPV training. Only 29.1% of the PHCPs reported a good level of perceived preparedness, 12.2% had good perceived knowledge, and a mere 8.6% had good actual knowledge. The perceived and actual knowledge, workplace/self-efficacy, and staff constraints scores were positively associated with the preparedness score, whereas the victim understanding score was negatively associated with the preparedness score. Multivariable logistic regression analysis revealed that longer work experience (≥ 10 years) (AOR = 1.70, 95% CI: 1.28-2.26), prior IPV training (AOR = 1.68, 95% CI: 1.12-2.51), previous experience with IPV inquiry (AOR = 1.55, 95% CI: 1.10-2.19), good perceived knowledge (AOR = 15.21, 95% CI: 11.15-20.74), and good actual knowledge (AOR = 1.79, 95% CI: 1.10-2.94) were significantly associated with a good level of perceived preparedness.

    CONCLUSIONS: A high percentage of Malaysian PHCPs have not received IPV training, and only a small proportion felt they are prepared to manage IPV, with even fewer possessing adequate knowledge about IPV. These findings highlight the urgent need to prioritize IPV training programs at the primary care level to better equip PHCPs with the knowledge and skills necessary to manage IPV effectively.

    MeSH terms: Adult; Attitude of Health Personnel; Clinical Competence; Cross-Sectional Studies; Female; Health Personnel/psychology; Humans; Health Knowledge, Attitudes, Practice; Malaysia; Male; Middle Aged; Primary Health Care*; Surveys and Questionnaires
  14. Genitsarios I, Jhagra R, Warn C, Xavier JA
    BMC Med Educ, 2025 Mar 31;25(1):465.
    PMID: 40165199 DOI: 10.1186/s12909-025-06695-w
    BACKGROUND: The incorporation of simulation-based learning in healthcare education, particularly in radiotherapy, is necessary for enhancing training and professional competencies to serve patient safety and treatment accuracy. This study aimed to incorporate an innovative end-to-end case study methodology, utilizing an anthropomorphic head phantom, into an undergraduate radiotherapy program at a United Kingdom (UK) based university. The objective was to enhance students' practical learning and theoretical understanding in radiotherapy treatment planning, a field where precision and accuracy are paramount.

    METHODS: The study began with an exploratory literature review to identify key educational challenges and opportunities in radiotherapy treatment planning. A qualitative approach was employed, using a focus group methodology to gather in-depth insights from subject experts, including educational and clinical professionals involved in undergraduate radiotherapy teaching. The focus group discussions explored the integration of an anthropomorphic head phantom within a simulated, case study-based training framework. This innovative approach combined practical skills development with theoretical learning, promoting active engagement and mirroring real-world clinical scenarios.

    RESULTS: Focus group discussions showed favorability towards the end-to-end case study method in simulation-based learning. Participants emphasized evaluating plans through assessments and using supplementary tools like video guides and workbooks to enhance learning. Incorporating the anthropomorphic phantom marked a notable advancement, offering authentic training possibilities in radiotherapy undergraduate education.

    CONCLUSIONS: The study demonstrates the potential of integrating an end-to-end teaching concept in radiotherapy education. By providing a realistic and comprehensive training experience, the approach can further enhance student engagement and learning outcomes. While real-world testing is pending, this innovative methodology shows promise in shaping proficient future radiotherapy graduates, highlighting the need for continuous evolution in educational strategies to meet the demands of modern healthcare training.

    MeSH terms: Simulation Training; Clinical Competence; Curriculum; Great Britain; Humans; Focus Groups; Radiation Oncology/education; Phantoms, Imaging*
  15. Chan L, Yu EYT, Wan EYF, Wong SYS, Chao DVK, Ko WWK, et al.
    BMC Med, 2025 Mar 31;23(1):190.
    PMID: 40165254 DOI: 10.1186/s12916-025-04007-z
    BACKGROUND: Delayed diagnosis and treatment of type 2 diabetes increases diabetes-related complications, making the high prevalence of undiagnosed type 2 diabetes in Hong Kong an important concern. Point-of-care capillary HbA1c (POC-cHbA1c) testing holds promise as a comparably accurate, convenient, and timely alternative to venous HbA1c (vHbA1c) for type 2 diabetes screening, yet randomized trials are lacking. This study compared the effectiveness of a 2-step active opportunistic screening strategy using POC-cHbA1c versus usual practice employing vHbA1c and multiple clinic visits in detecting type 2 diabetes among at-risk primary care patients. The primary outcomes were to identify the difference in the proportion of type 2 diabetes detected between intervention (POC-cHbA1c) and control (vHbA1c) groups and the uptake rate of POC-cHbA1c versus vHbA1c testing among consenting participants.

    METHODS: A cluster randomized controlled trial was conducted in 8 General Out-Patient Clinics between June 2022 and January 2024 using 2-step active opportunistic screening. In step 1, risk factor count, 852 at-risk patients were identified through consecutive sampling during their primary care consultation by specific inclusion and exclusion criteria. In step 2, these at-risk patients then underwent POC-cHbA1c (intervention) or vHbA1c (control) testing. If preliminary HbA1c was ≥ 5.6%, a confirmatory oral glucose tolerance test was offered. Randomization occurred at the clinic level using a random allocation sequence generated by statistical software. Multilevel logistic regression analyses were employed to evaluate the effect of the intervention on the uptake rate, adjusting for patient characteristics and clinic clustering.

    RESULTS: POC-cHbA1c had a higher uptake rate than vHbA1c (76.0% vs 37.5%; OR = 7.06, 95% CI [2.47-20.18], p 

    MeSH terms: Adult; Aged; Female; Hong Kong/epidemiology; Humans; Male; Middle Aged; Primary Health Care
  16. Athirah DN, Asaruddin MR, Bhawani SA, Simbas AF, Jack KS
    BMC Res Notes, 2025 Mar 31;18(1):136.
    PMID: 40165289 DOI: 10.1186/s13104-024-07039-5
    OBJECTIVES: This study aimed to evaluate the effectiveness of the E' food grade preservative - potassium sorbate (E202) and sodium metabisulfite (E223) in an aqueous form, and impact of the timing of preservative addition on the shelf-life, sugar and reducing sugars levels, organic acids content and microbial growth of nipa palm sap.

    RESULTS: The pH, sugar and reducing sugars levels, organic acids content, total bacterial count (TBC) and sensory characteristics of the sample were determined. The results showed that the treated samples were able to slow down the pH and sucrose content from dropping during storage. Sample A had lactic acid at 0.93 ± 0.01 g/L and acetic acid at 0.05 ± 0.0.1 g/L, while Sample B had 0.97 ± 0.02 g/L and 0.07 ± 0.01 g/L, respectively, both lower than the control (1.10 ± 0.01 g/L and 0.09 ± 0.02 g/L). Sample A with TBC of 1.03 (± 0.08) x 105 CFU/mL was able to inhibit microbial growth better than Sample B, 1.14 (± 0.13) x 105 CFU/mL. Preservative application prior to tapping was shown to be more successful in preserving the quality and shelf-life of nipa palm sap. This approach is likely preventing early microbial activity and fermentation without the need of proper packaging, preserving the freshness and quality of the sap more effectively than adding the preservative after collection.

    MeSH terms: Food Microbiology/methods; Humans; Hydrogen-Ion Concentration; Arecaceae/microbiology; Arecaceae/chemistry; Food Storage/methods
  17. Debnath SC, Chaput DL, McMurtrie J, Bell AG, Temperton B, Mohan CV, et al.
    Environ Microbiome, 2025 Mar 31;20(1):38.
    PMID: 40165346 DOI: 10.1186/s40793-025-00687-5
    BACKGROUND: The pondwater microbiome is believed to play a key role in fish health, including shaping mucosal surface microbiomes that help to protect against disease. How different physiochemical features relating to season, geographical locations, as well as crop species shape the pond water microbiome in the finfish aquaculture system, is not well established. Pangasius (Pangasianodon hypophthalmus) and tilapia (Oreochromis niloticus) are two of the most widely farmed fish species and disease is a major impediment to the expansion of their production. We applied 16S and 18S rRNA metabarcoding to assess how pond physicochemistry and geographical location shape water microbiomes in pangasius and tilapia aquaculture earthen ponds in Bangladesh.

    RESULTS: Planctomycetota, Pseudomonadota and Actinomycetota were the dominant bacterial phyla while Stramenopiles and Alveolata were the dominant microeukaryotes (divisions) in the pangasius and tilapia ponds water. The relative abundance of Planctomycetota was higher in the pangasius ponds compared with tilapia ponds, and Actinomycetota, and Pseudomonadota were relatively higher in tilapia ponds. Tilapia pond water also exhibited a higher microbial diversity compared to that in pangasius ponds. The pondwater microbial diversity was at its lowest in winter (and/or in monsoon) and highest in the pre-monsoon period. The microbial community structures differed across the different seasons, geographical locations, culture systems, and crop species, with season and geographical locations showing the strongest effects. Of the water physicochemistry features assessed, temperature and pH were found to have a weak but significant effect on the water microbiome content for both pangasius and tilapia ponds. Pangasius and tilapia ponds shared over 46% of ASVs, and around 30% of ASVs were shared across the different study geographical locations.

    CONCLUSION: Our findings demonstrate that microbial communities in pangasius and tilapia aquaculture systems in Bangladesh are shaped by season, geographical location, crop species, as well as effects from water physicochemistry. Our results provide insights into the dynamic nature and environmental influences on water microbiomes that may be applied for use in pond management for improving aquaculture productivity and enhancement of overall fish health.

  18. Chua HB, Hussain RI, Shukor NA, Fam XI
    Front Oncol, 2025;15:1489833.
    PMID: 40165894 DOI: 10.3389/fonc.2025.1489833
    Retroperitoneal liposarcoma, especially dedifferentiated liposarcoma (DDL), is a rare tumor type primarily affecting middle-aged and older adults in the retroperitoneum or proximal extremities. This case report highlights an exceptionally large retroperitoneal DDL that had enveloped the entire right kidney and had adhered to nearby tissues. Diagnosing retroperitoneal liposarcoma is challenging due to its asymptomatic nature until it reaches a substantial size. Imaging, particularly contrast-enhanced computed tomography (CECT), play a vital role in diagnosis, staging, and preoperative planning. Surgical resection, with the goal of R0 resection, remains the cornerstone of treatment, albeit this can be challenging due to tumor location. First-line treatment for advanced DDL involves anthracycline-based therapy. Eribulin and pazopanib show promise in second-line treatment. Ongoing clinical trials suggest a shift towards multimodal therapy. This case report reports the largest retroperitoneal liposarcoma and underscores the complexity of managing retroperitoneal DDL.
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