Browse publications by year: 2025

  1. Daood U, Ilyas MS, Bashir S, Yousuf N, Rashid M, Kaur K, et al.
    Int Dent J, 2025 Apr;75(2):1277-1291.
    PMID: 39322516 DOI: 10.1016/j.identj.2024.09.012
    AIMS AND OBJECTIVES: To examine if a novel antimicrobial silane K21 can alter macrophage polarisation and affect fibroblast proliferation by deciphering the molecular pathways for programmed healing using a combined in vitro and in vivo (animal) burn model.

    MATERIALS AND METHODS: An injectable silane-based antimicrobial aimed to modulate macrophage polarisation was manufactured. Experimental analysis included colorimetric cell migration assays on gingival fibroblasts, macrophage phagocytosis characterisation, immunofluorescence staining, triacylglycerol accumulation within macrophages by LCMS, cellular metabolic/proliferation assays, macrophage exposure quantification with morphology assessment using FE-SEM, Raman spectral analysis, RNA isolation for relative gene expression and animal study model to morphometrically and microscopically analyse partial thickness burn wound healing under QAS/K21.

    RESULTS: M1 and M2 polarisation both appeared exaggerated under QAS/K21 treatment. The wounds treated with K21 had depicted accelerated healing as compared to control (P < .05) in dorsal skin of rabbits. Relative gene expression results demonstrate reduced cytokine and anti-inflammatory response under the influence of K21. While M1 expression, TG accumulation, and associated characterisations demonstrate the programmed inflammatory potential of K21.

    CONCLUSION: the antimicrobial and reparative efficacy of K21 silane aids in programmed inflammation for enhanced tissue healing and repair.

    MeSH terms: Animals; Cell Movement/drug effects; Disease Models, Animal; Humans; Inflammation; Phagocytosis/drug effects; Rabbits; Cell Proliferation/drug effects
  2. Nezhad NG, Buhari SB, Eskandari A, Albayati SH, Omotayo OF, Leow TC
    3 Biotech, 2025 Apr;15(4):93.
    PMID: 40124131 DOI: 10.1007/s13205-025-04258-w
    The current in silico investigation aimed to increase the thermostability of IsPETase for more efficient PET degradation. N-Truncated and S209H mutants were designed to improve the thermostability of IsPETase. The deletion of the first seven N-terminal residues in PETase (N-truncated mutant) disrupts structural integrity, as Arg5, a crucial residue, maintains stability by forming a hydrogen bond network with Pro2, Thr48, Lys230, and Arg238. This links the N-terminal to the C-terminal, while its absence increases RMSF values in this region. The S209H mutation, located in the catalytic loop of IsPETase, enhances thermostability by introducing a new hydrophobic interaction with residue W130. MD simulations at 353.15 K have demonstrated this improvement, showing reduced structural flexibility and compactness in the S209H mutant compared to the WT. Specifically, the overall RMSD, Cα RMSD, SASA, and Rg values decreased from 3.36249 ± 0.853 Å, 1.321843 ± 0.0953 Å, 10,057.73 ± 135.11 Å2, and 17.09687 ± 1.387 Å in the WT to 3.184878 ± 0.786 Å, 0.969998 ± 0.119 Å, 9894.527 ± 118.53 Å2, and 16.962 ± 1.265 Å in the S209H mutant, respectively. Molecular docking revealed binding energies of -4.9 kcal/mol for WT, -5.1 kcal/mol for the S209H mutant, and -4.8 kcal/mol for the N-truncated mutant. MM-PBSA analysis using YASARA showed that the S209H mutation increased binding energy from -17.3606 kJ/mol (WT) to -7.82077 kJ/mol, enhancing binding affinity, while the N-truncated mutant reduced binding energy to -23.5032 kJ/mol, lowering binding affinity. In conclusion, this study has demonstrated that the S209H mutation enhanced the thermostability and the PET affinity of IsPETase by introducing the hydrophobic interactions. The N-truncated mutant reduced both thermostability and PET affinity, highlighting the critical role of the N-terminal region in maintaining the stability and activity of IsPETase.
  3. Eziwar Dyari HR, Muhammad Rasib MA, Abu Bakar J, Suriyanti SNP
    Data Brief, 2025 Apr;59:111401.
    PMID: 40124284 DOI: 10.1016/j.dib.2025.111401
    Environmental DNA (eDNA) is a very useful method for studying the biodiversity as it obtains genetic materials from environmental samples such as soil, water and air. This highly sensitive detection could attain information despite the scarcity of population densities. This non-invasive approach is feasible particularly in impact assessment of marine development. Hybrid Ocean Thermal Energy Conversion (H-OTEC) is a renewable energy technology that generates electricity by using sea water temperature differentials. While the H-OTEC is currently in its pre-commissioning state, it is crucial to provide a baseline data on the marine biota in this site. This study targets the 18s rRNA genes of the marine metazoans at the H-OTEC plant site at Port Dickson, Negeri Sembilan, Malaysia. Ten litres of seawater were collected from the site and immediately filtered through a 0.2 µm cellulose membrane filter. The eDNA extraction was carried out using cetyltrimethylammonium bromide (CTAB) protocol and sent for next generation sequencing. High-throughput sequencing using the Illumina platform produced 113,980 filtered reads, corresponding to 73 genera, 64 families, and 6 phyla of marine metazoans in the area under examination. The highest relative abundance belongs to the phylum Arthropoda (85%), family Paracalanidae (45%) and genus Bestiolina (38%). The diversity indices indicate a great deal of biodiversity in the ecosystem.
  4. Nain RA, Thomas DC, Makajil JD, Fauziningtyas R
    Data Brief, 2025 Apr;59:111416.
    PMID: 40124290 DOI: 10.1016/j.dib.2025.111416
    This dataset contains information on knowledge retention and learning satisfaction in the context of patient-nurse safety education. The data was collected through structured questionnaires administered to participants before and after implementing a safety-focused learning module. The dataset includes demographic variables, pre- and post-assessment scores measuring knowledge retention, and feedback on participants' satisfaction with the learning experience. Data were compiled to facilitate the identification of patterns, relationships, and associations between knowledge acquisition and satisfaction with educational interventions. The dataset is presented in a tabular format to enable further analysis and application in educational program evaluations or other related fields. The reuse potential includes informing curriculum development, identifying effective teaching strategies, and benchmarking learning outcomes.
  5. Vo QV, Hibino Y, Ho HC, Le TTT, Seah YG
    Zookeys, 2025;1231:311-329.
    PMID: 40124315 DOI: 10.3897/zookeys.1231.137323
    Two new extremely elongate snake eel of the genus Ophichthus are described based on specimens collected from Vietnamese waters. Ophichthuscuulongensis Vo, Hibino & Ho, sp. nov. is distinguished from its congeners by having the dorsal-fin origin slightly behind the pectoral-fin tip, mean vertebral formula 14-63-202, range 12-17/60-64/199-207; teeth on jaws biserial to triserial; dorsal body dark brown, ventral body pale, anal fin initially white but changing to darker towards its tip. Ophichthusnguyenorum Vo, Hibino & Ho, sp. nov. is distinguished by having a snout rather pointed but the occipital convex (duck-shaped); body with numerous longitudinal wrinkles, weak on posterior abdomen; dorsal-fin origin slightly behind the pectoral-fin tip; one row of teeth on the maxilla anteriorly but increasing posteriorly; two rows on the lower jaw; all teeth small; body dark, usually including abdomen; dorsal fin darker with dark margin; anal fin initially pale but changing to darker towards tip; mean vertebral formula: 15-62-192, range 13-17/61-64/190-196. Descriptions of two related species, O.macrochir (Bleeker, 1852) and O.rutidoderma (Bleeker, 1852), are provided with updated morphological data.
  6. J J, Haw SC, Palanichamy N, Ng KW, Aneja M, Taiyab A
    MethodsX, 2025 Jun;14:103247.
    PMID: 40124330 DOI: 10.1016/j.mex.2025.103247
    In this work, the CT scans images of lung cancer patients are analysed to diagnose the disease at its early stage. The images are pre-processed using a series of steps such as the Gabor filter, contours to label the region of interest (ROI), increasing the sharpening and cropping of the image. Data augmentation is employed on the pre-processed images using two proposed architectures, namely (1) Convolutional Neural Network (CNN) and (2) Enhanced Integrated model for Lung Tumor Identification (EIM-LTI).•In this study, comparisons are made on non-pre-processed data, Haar and Gabor filters in CNN and the EIM-LTI models. The performance of the CNN and EIM-LTI models is evaluated through metrics such as precision, sensitivity, F1-score, specificity, training and validation accuracy.•The EIM-LTI model's training accuracy is 2.67 % higher than CNN, while its validation accuracy is 2.7 % higher. Additionally, the EIM-LTI model's validation loss is 0.0333 higher than CNN's.•In this study, a comparative analysis of model accuracies for lung cancer detection is performed. Cross-validation with 5 folds achieves an accuracy of 98.27 %, and the model was evaluated on unseen data and resulted in 92 % accuracy.
  7. Teoh RJJ, Yogesvaran R, Cheng FFY, Muji R
    Oman J Ophthalmol, 2025;18(1):28-32.
    PMID: 40124440 DOI: 10.4103/ojo.ojo_223_23
    BACKGROUND: The aim of the study was to assess the midterm efficacy and safety of the iStent glaucoma device in patients with open-angle glaucoma (OAG).

    MATERIALS AND METHODS: We conducted a retrospective case series of all patients implanted with iStent inject® in OAG. Primary outcomes included intraocular pressure (IOP) and the reduction of antiglaucoma medications. Safety outcomes comprised adverse complications, additional surgeries, and postoperative best-corrected visual acuity (BCVA).

    RESULTS: Eight eyes from eight patients were included in this series. The mean age was 56.3 ± 17.5 years. Six eyes had primary OAG and two eyes had secondary OAG. The majority of eyes (75%) had mild-to-moderate glaucoma. Five eyes underwent combined surgery, whereas the remaining three eyes underwent a standalone procedure. The mean baseline IOP was 22.9 ± 8.2 mmHg, and the mean IOP at 2 years after the procedure was 13.6 ± 2.8 mmHg. There was a 44.5% (10.2 ± 2.5 mmHg; P = 0.002) IOP reduction over 2 years. There was also a reduction in medication burden from a baseline mean of 3.4 ± 0.7 to 2.0 ± 1.2 (P = 0.17). There was no documented intraoperative adverse complication, whereas one patient required trabeculectomy post iStent for IOP control.

    CONCLUSION: iStent inject implantation safely reduced IOP and medication burden up to 24 months postoperative.

  8. Wang T, Liu Y, Wang X, Amri S, Kamalden TF, Gao Z, et al.
    PeerJ, 2025;13:e19110.
    PMID: 40124607 DOI: 10.7717/peerj.19110
    BACKGROUND: Low-load blood flow restriction (LL-BFR) training has been shown to enhance muscle strength, power, and speed, but its effectiveness compared to traditional high-load resistance (HLR) training remains unclear. This meta-analysis aimed to compare the effects of LL-BFR and HLR training on muscle strength, power, and speed.

    METHODOLOGY: Studies were identified by searching the SCOPUS, SPORTDiscus, PubMed, Web of Science, and CNKI databases up to May 13, 2024, using the following inclusion criteria: (a) healthy population; (b) comparison of LL-BFR vs HLR training; (c) pre- and post-training assessment of muscle strength (dynamic, isometric, and isokinetic), muscle power, jump, or speed performance; (d) PEDro scale score ≥4. The methodological quality of the included studies was assessed using the PEDro tool and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach, with meta-analyses conducted using the R program.

    RESULTS: A total of 41 studies, involving 853 subjects, were included in the meta-analysis. Based on the PEDro scores and GRADE assessment, the overall quality of the included studies was assessed as moderate. LL-BFR training showed a slightly smaller effect on maximal strength compared to HLR training (ES = -0.19, 95% CI [-0.31 to -0.06], p < 0.01). There were no significant differences between LL-BFR and HLR training for muscle power (ES = -0.04, 95% CI [-0.33 to 0.24], p > 0.05), jump performance (ES = -0.08, 95% CI [-0.30 to 0.15], p > 0.05), and speed (ES = -0.28, 95% CI [-0.71 to 0.15], p > 0.05). Additionally, individual characteristics (i.e., age, gender, and training status) and training parameters (i.e., training duration, frequency, cuff pressure, and cuff width) did not significantly moderate the training effect.

    CONCLUSIONS: LL-BFR training showed slightly less improvement in maximal strength compared to HLR training but demonstrated comparable effects on muscle power, jump performance, and speed in healthy individuals in healthy individuals. These findings suggest that LL-BFR may be a practical and effective alternative for individuals seeking performance improvements with lower training loads.

    MeSH terms: Humans; Regional Blood Flow/physiology; Muscle, Skeletal/blood supply; Muscle, Skeletal/physiology; Healthy Volunteers
  9. Choong Yoke Lin C
    Cureus, 2025 Feb;17(2):e79210.
    PMID: 40125192 DOI: 10.7759/cureus.79210
    Fingertip amputations are complex injuries that may involve the loss of soft tissue, pulp, nailbed, tendon, and bone. Many surgical techniques have been described over the years to address these fingertip defects. The choice and method of defect coverage depends on the amount of soft tissue and bone loss. We present a case series of eight fingertip amputations that were treated with oblique triangular flap coverage. Our patients had a minimum of two months follow-up and were assessed at one year postoperatively. The favorable results observed in this series have reinforced our confidence in the reliability of this treatment technique for fingertip amputations in our Malaysian population.
  10. Chua RHB, Loo GH, Hl N, Muthkumaran G, Ritza Kosai N
    Cureus, 2025 Feb;17(2):e79331.
    PMID: 40125210 DOI: 10.7759/cureus.79331
    Failed back surgery syndrome (FBSS) poses a significant challenge in chronic pain management, particularly in patients with obesity, where excess weight exacerbates spinal strain and pain. Bariatric surgery has been explored as a potential adjunctive treatment for pain relief by reducing mechanical stress and systemic inflammation. We report the case of a 42-year-old woman with a history of FBSS, chronic back pain, narcolepsy, rheumatoid arthritis, and obesity, who underwent laparoscopic sleeve gastrectomy as part of a multidisciplinary pain management strategy. Despite having a spinal cord stimulator for pain control, she remained dependent on opioids, gabapentin, and amitriptyline. Following an uneventful surgery, postoperative pain was effectively managed with a ketamine infusion, leading to significant pain score reduction. The patient was discharged with an optimized pain regimen and demonstrated early improvements in mobility and overall well-being. This report highlights the potential role of bariatric surgery in managing chronic pain in patients with FBSS and obesity. It underscores the importance of a multidisciplinary approach, preoperative planning, and tailored postoperative pain management in optimizing outcomes. Further research is warranted to evaluate the long-term impact of bariatric surgery on chronic pain management and functional recovery in this patient population.
  11. Li Y, Soh KL, Jing X, Wei L, Saidi HI, Soh KG
    J Multidiscip Healthc, 2025;18:1655-1678.
    PMID: 40125304 DOI: 10.2147/JMDH.S505135
    PURPOSE: This study aims to conduct a bibliometric analysis of research literature on psychological interventions for stroke survivors published from 2000 to 2024, focusing on psychological resilience and psychological well-being, utilizing VOSviewer and CiteSpace.

    PATIENTS AND METHODS: Literature data was sourced from the Web of Science Core Collection database (WoSCC). A total of 373 relevant articles published between January 1, 2000, and September 30, 2024, were included. CiteSpace and VOSviewer analyzed the literature from perspectives including authorship, country of origin, institutions, journals, references, and keywords.

    RESULTS: Annual publication output has steadily increased, reaching a peak in 2023 (55 articles). Vranceanu Ana-Maria emerged as the most productive author with eight publications. The United States led in research output (98 articles) and centrality (0.32), with Harvard University being the leading institution (24 articles). "Disability and Rehabilitation" was the most productive journal (12 articles), while "Stroke" was the most co-cited journal (241 times). Recent research trends emphasized meta-analysis (strength=3.6), assessment tool validation (strength=3.49), and acceptance-based interventions (strength=2.89), mainly focusing on psychological resilience cultivation and well-being promotion.

    CONCLUSION: This bibliometric analysis reveals the increasing scholarly interest in psychological interventions for stroke survivors, particularly in resilience and psychological well-being research. The field has evolved from focusing on disease-related factors to systematic intervention research, with a growing emphasis on methodological standardization and individualized interventions. These findings provide significant theoretical and practical implications for improving psychological health services for stroke survivors. Future research should strengthen high-quality empirical studies, refine assessment tools, and innovate intervention strategies to better address the complex psychological needs of stroke survivors and enhance their resilience and psychological well-being, ultimately improving rehabilitation outcomes and quality of life for stroke survivors.

  12. Ruedas-Torres I, Findlay-Wilson S, Kennedy E, Dowall S, Salguero FJ
    Front Vet Sci, 2025;12:1518358.
    PMID: 40125323 DOI: 10.3389/fvets.2025.1518358
    Nipah virus (NiV) is recognized as one of the key pathogens with pandemic potential. We have recently established a NiV hamster model, which reproduces a highly similar disease to that observed in human cases, including respiratory and neurological signs and lesions. The aims of this study were to describe the microscopic lesions observed in the golden Syrian hamster model after intranasal (IN) and intraperitoneal (IP) inoculation with different doses of the Malaysian strain of NiV; to describe in depth the cell composition of the pulmonary and the brain lesions and the expression of proinflammatory cytokines in-situ using a combination of histopathological techniques including immunohistochemistry (IHC) and in-situ hybridisation (ISH) via RNAscope technique. We also developed a multiplex IHC which will allow us to study the interaction of the virus with cell populations in the lung and brain in future studies. For this, we selected 28 lung and brain formalin-fixed paraffin-embedded (FFPE) samples from previous experiments performed by our research group. Histopathology revealed severe pulmonary broncho-interstitial pneumonia, mainly in animals inoculated via the IN route, accompanied by a strong acute inflammatory response (Iba1+ cells) and high levels of NiV RNA. Upregulation of proinflammatory cytokines (IL-6 and TNF) was also observed by ISH RNAscope technique in these animals. Neurological lesions, consisting of perivascular cuffing and meningitis, were observed mainly in animals inoculated via IP route. IHC results showed astrocytosis (GFAP+) and microgliosis (Iba1+) in the brain of these animals, together with mild levels of IL6 and TNF mRNA. These results have helped us to characterize the host-pathogen interaction in the golden Syrian hamster animal model of NiV infection that is being currently used in preclinical testing of antiviral and vaccine strategies. Techniques used in this study could be applied to the development and application of golden Syrian hamster models of other infections by henipaviruses, including Hendra virus (HeV), and other high consequence priority pathogens.
  13. Itsukushima R, Adnan MS, Tomiyama Y, Kano Y, Otsu K, Zanorin MF
    Biodivers Data J, 2025;13:e148173.
    PMID: 40125406 DOI: 10.3897/BDJ.13.e148173
    BACKGROUND: Urbanisation leads to the degradation of ecosystems through various factors, such as the deterioration of water quality, changes in water and material cycles and the degradation of biological habitats. Amongst these, aquatic organisms are particularly affected by the loss of habitats due to river canalisation and the impacts of invasive species. It has been widely reported that, in regions where invasive species have been introduced and native species have declined, homogenisation of fish populations occurs, resulting in a significant reduction in biodiversity. This loss of diversity disrupts the ecosystem's stability and resilience, further compounding the negative effects of urbanisation on aquatic environments. However, the impact of urbanisation on fish populations varies depending on the local ecosystem and the degree of urbanisation, necessitating the examination of ecosystem changes induced by urbanisation in each specific region. The Peninsula Malaysia, which is the focus of this study, is a global hotspot for freshwater biodiversity. However, the effects of urbanisation on fish populations in this region have been scarcely studied. The Masai River Basin, which is the subject of this investigation, is located in the Iskandar Development Region, an area undergoing rapid urbanisation. Understanding the consequences of urbanisation on the fish populations and broader ecosystems in this region is critical for providing information for future conservation and management strategies.

    NEW INFORMATION: A fish survey was conducted at 19 sites in the Masai River Basin, which is an urbanised watershed, focusing on river channels that have been straightened or converted into concrete-lined waterways. Additionally, fish surveys were conducted at eight sites in non-urbanised areas for comparison. The survey resulted in the collection of nine orders, 15 families, 28 genera, 32 species and a total of 3,007 individuals. In the urbanised sites, the proportion of native species in the total catch was extremely low, averaging only 10.4% across all sites, with invasive species making up the majority of the individuals captured. This indicates the significant shift in species composition due to urbanisation and the dominance of non-native species in these environments. On the other hand, in the non-urbanised areas, the proportion of native species was high at 88.7%, highlighting the significant impact of urbanisation on the invasion of non-native species. Particularly in the downstream areas of the urbanised watershed, species such as Poeciliasphenops, Mayaherosurophthalmus and Poeciliareticulata were frequently captured. In contrast, at sites in the upstream areas where forested habitats remained intact, native species listed on the IUCN Red List, such as Parambassissiamensis and Clariasbatrachus, were captured. The study revealed that urbanisation and development in the targeted watershed are progressing rapidly, underscoring the urgent need for the conservation and restoration of habitats for these native species.

  14. Brutus JP, Ahmad AA, Apard T, Tchiloemba B, Chang MC, Lalonde DH
    Plast Reconstr Surg Glob Open, 2025 Mar;13(3):e6568.
    PMID: 40125471 DOI: 10.1097/GOX.0000000000006568
    BACKGROUND: Minimally painful tumescent local anesthesia ensures patients feel only the first needle insertion, with no further pain. This technique includes real-time patient feedback, where they report each pain event during injection.

    METHODS: This prospective study involved 154 consecutive patients undergoing wide-awake local anesthesia no tourniquet surgery at 3 hand surgery centers (January-April 2024). Patients objectively scored pain events during injection and rated pain intensity (0-10 Likert scale), intraoperative pain, anxiety, and overall experience.

    RESULTS: During local anesthesia injection, 61 (40%) patients reported no pain, 92 (59.7%) reported 1 pain event, and 1 (0.7%) patient reported 2 events. Among the 93 patients who felt pain, 90 reported only mild discomfort (1-2 of 10), whereas 3 reported moderate pain (3-5 of 10). Anxiety levels during anesthesia and surgery were 3 of 10 or less for 147 (95.5%) patients.

    CONCLUSIONS: Real-time patient feedback improved surgeons' ability to administer tumescent local anesthesia with minimal pain. As a result, most patients experienced no pain or only 1 minor event during local anesthesia injection for wide-awake local anesthesia no tourniquet surgery.

  15. Abid SK, Sulaiman N, Samman B, Al-Wathinani AM, Goniewicz K
    Disaster Med Public Health Prep, 2025 Mar 24;19:e69.
    PMID: 40125665 DOI: 10.1017/dmp.2025.68
    OBJECTIVE: Disaster management strategies often emphasize technical and structural solutions, overlooking the sociocultural factors that shape community resilience and disaster response. In Malaysia, a multiethnic and multireligious country frequently affected by floods and monsoon storms, cultural beliefs, social networks, and traditional practices play a pivotal role in shaping disaster preparedness and recovery. This study examines how religious beliefs, community cohesion, gender roles, and traditional knowledge influence disaster management in Malaysia.

    METHODS: A qualitative research approach was employed, utilizing semi-structured interviews with 15 stakeholders from diverse ethnic, religious, and social backgrounds. Participants, represented various religious groups and geographic areas. Their roles included local leaders, government officials, NGO workers, and community members, providing insights into how sociocultural factors influence disaster response and policy.

    RESULTS: Religious beliefs serve as both a source of resilience and a potential barrier, shaping community attitudes toward disaster preparedness. Community cohesion, particularly through gotong-royong (mutual aid), plays a crucial role in mobilizing resources and support, though it often excludes marginalized groups. Gender roles significantly influence disaster response, with women taking on caregiving responsibilities yet remaining underrepresented in decision-making processes. Traditional knowledge remains valuable, particularly in rural communities, but faces challenges as younger generations increasingly rely on modern technologies.

    CONCLUSIONS: This study highlights the need for culturally sensitive, gender-inclusive, and community-driven disaster management policies in Malaysia.Integrating sociocultural dimensions into formal frameworks can foster more adaptive and inclusive strategies. Enhancing community participation and gender inclusivity will be key to improving disaster resilience in Malaysia.

    MeSH terms: Adult; Disaster Planning/methods; Female; Humans; Interviews as Topic/methods; Malaysia/ethnology; Male; Middle Aged; Qualitative Research*
  16. Villalobos ME, Vivanti G, Jayanath S, Carpenter K, Shen MD, Shic F, et al.
    Autism Res, 2025 Mar 24.
    PMID: 40125848 DOI: 10.1002/aur.70028
    The International Society for Autism Research (INSAR) was established in 2001 with the purpose of providing researchers in the field of autism a specific venue to enhance the quality of multidisciplinary research and elevate the field among the sciences. The autism field and size of the society has now expanded greatly to include over 5000 members, 29% of whom identify as early career researchers. In 2016, a survey was conducted with these early career researchers to identify existing supports and areas of need necessary for a successful and sustained autism research career. Results clearly identified three areas of need: (1) networking opportunities with "change makers," including funding agencies, journal editors, and senior autism researchers; (2) ensuring early career researchers in the autism research field were diverse and representative of the world; and (3) support from senior researchers to help early career researchers secure their first independent grant and move through promotion. The INSAR Early Career Committee (ECC) was established and developed three initiatives to address the aforementioned gaps: Research Rapid Rounds, the Global Representatives Initiative Pilot (GRIP), and the Mentoring Initiative. The ECC has successfully connected over 34 early career researchers and 40 students/trainees to mentors in 19 unique countries through networking programs and events and has established representatives in seven different countries outside the US and UK. These initiatives are a step towards supporting early career academics in the autism field and bring together autism researchers from around the world to share their work and create professional collaborations to forge our field forward.
  17. Sun Y, Puspanathan P, Lim T, Lin D
    Cancer Biol Med, 2025 Mar 24.
    PMID: 40126094 DOI: 10.20892/j.issn.2095-3941.2024.0386
    Advances in the identification of molecular biomarkers and the development of targeted therapies have enhanced the prognosis of patients with advanced gastric cancer. Several established biomarkers have been widely integrated into routine clinical diagnostics of gastric cancer to guide personalized treatment. Human epidermal growth factor receptor 2 (HER2) was the first molecular biomarker to be used in gastric cancer with trastuzumab being the first approved targeted therapy for HER2-positive gastric cancer. Programmed death-ligand 1 positivity and microsatellite instability can guide the use of immunotherapies, such as pembrolizumab and nivolumab. More recently, zolbetuximab has been approved for patients with claudin 18.2-positive diseases in some countries. More targeted therapies, including savolitinib for MET-positive patients, are currently under clinical investigation. However, the clinical application of these diagnostic approaches could be hampered by many existing challenges, including invasive and costly sampling methods, variability in immunohistochemistry interpretation, high costs and long turnaround times for next-generation sequencing, the absence of standardized and clinically validated diagnostic cut-off values for some biomarkers, and tumor heterogeneity. Novel testing and analysis techniques, such as artificial intelligence-assisted image analysis and multiplex immunohistochemistry, and emerging therapeutic strategies, including combination therapies that integrate immune checkpoint inhibitors with targeted therapies, offer potential solutions to some of these challenges. This article reviews recent progress in gastric cancer testing, outlines current challenges, and explores future directions for biomarker testing and targeted therapy for gastric cancer.
  18. Awomuti A, Yu Z, Adesina O, Samuel OW, Mumbi AW, Yin D
    SAR QSAR Environ Res, 2025 Mar 24.
    PMID: 40126364 DOI: 10.1080/1062936X.2025.2478123
    Peroxisome proliferator-activated receptor gamma (PPARγ), a critical nuclear receptor, plays a pivotal role in regulating metabolic and inflammatory processes. However, various environmental contaminants can disrupt PPARγ function, leading to adverse health effects. This study introduces a novel approach to predict the inhibitory activity (IC50 values) of 140 chemical compounds across 13 categories, including pesticides, organochlorines, dioxins, detergents, flame retardants, and preservatives, on PPARγ. The predictive model, based on the light-gradient boosting machine (LightGBM) algorithm, was trained on a dataset of 1804 molecules showed r2 values of 0.82 and 0.59, Mean Absolute Error (MAE) of 0.38 and 0.58, and Root Mean Square Error (RMSE) of 0.54 and 0.76 for the training and test sets, respectively. This study provides novel insights into the interactions between emerging contaminants and PPARγ, highlighting the potential hazards and risks these chemicals may pose to public health and the environment. The ability to predict PPARγ inhibition by these hazardous contaminants demonstrates the value of this approach in guiding enhanced environmental toxicology research and risk assessment.
  19. Albaharna H, Alrasheed A, AlQahtani A, Psaltis AJ, Javer A, Meco C, et al.
    PMID: 40126454 DOI: 10.1002/alr.23572
    BACKGROUND: Advancements in endoscopic sinus and skull base surgery created a need for standardized terminology to describe sphenoid sinus surgery. Although classification systems exist for other sinuses, one for endoscopic sphenoid sinus surgery is lacking. Developing such a system would standardize procedure descriptions and promote a common language among surgeons. This study aimed to develop a new classification system for endoscopic sphenoid surgery.

    METHODS: Consensus on a novel endoscopic sphenoid surgery classification system by running the Delphi procedure with 16 rhinology experts from around the world.

    RESULTS: Four Delphi rounds were required to reach a consensus on all stages of the classification. The average percentage of agreement on the stages of classification progressively increased from 70.83% in the first round to 87.68% in the last round. The rejection rates continuously decreased from 8.81% in the first round to 4.44% in the last round. The classification system was developed as follows: stage 1, presphenoid surgery; stage 2A, partial sphenoidotomy; stage 2B, complete sphenoidotomy; stage 2C, transpterygoid sphenoidotomy; stage 3A, Rostral sphenoidectomy; and stage 3B, extended sphenoid drill-out.

    CONCLUSIONS: This novel endoscopic sphenoid surgery classification system facilitates the description of different sphenoid sinus procedures, providing surgeons with better opportunities for discussion and communication.

  20. Tan SH, Yap YY, Tan SK, Wong CK
    JMIR Aging, 2025 Mar 24;8:e60936.
    PMID: 40126531 DOI: 10.2196/60936
    BACKGROUND: The aging population and the accompanying rise in chronic diseases have intensified the need to study the adoption of telehealth services. However, the success of telehealth services depends not only on their ease and usefulness but also on addressing broader concerns. Despite being a substantial user group in traditional health services, older adults may encounter barriers to adopting telehealth services. Increasing the adoption of telehealth among the older adult population is crucial for enhancing their access to care and managing the challenges of aging effectively.

    OBJECTIVE: We aimed to explore factors influencing the adoption of telehealth services among older adults in Malaysia, going beyond the conventional framework by incorporating transition cost and subjective well-being as additional constructs.

    METHODS: A cross-sectional survey was conducted among 119 adults aged ≥60 years in Malaysia, using 39 survey items adapted from existing studies. Data analysis was performed using partial least squares structural equation modeling, with both the measurement model and structural model being evaluated. To determine the predictive relevance of the model, PLSpredict was applied. In addition, importance-performance map analysis was conducted to further expand on the structural model results by assessing the performance of each variable.

    RESULTS: Of the 119 participants, 52 (43.7%) were women and 67 (56.3%) were men. The study found that subjective well-being (β=0.448; P

    MeSH terms: Aged; Aged, 80 and over; Cross-Sectional Studies; Female; Humans; Malaysia; Male; Middle Aged; Patient Acceptance of Health Care/psychology; Patient Acceptance of Health Care/statistics & numerical data; Surveys and Questionnaires
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